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| 1 | 间接荧光抗体法快速诊断海兰褐蛋鸡J亚群禽白血病的研究显示文摘某地区海兰褐蛋鸡发生肿瘤性传染病 ,经临床症状和病理学检查 ,在肝、脾、肾、卵巢、输卵管、肺、骨髓、腺胃、肠道等可见胞浆内充满球形嗜酸性颗粒的骨髓瘤细胞 ,呈灶状或弥漫性的分布。该变化是禽骨髓细胞瘤病病理学的特征性变化 ,具有证病意义。经用特异性抗 J亚群白血病病毒囊膜蛋白 gp85的单克隆抗体的免疫组化方法 ,在病料组织切片上检出病毒抗原 ,从而确诊为蛋鸡 J亚群禽白血病。本研究是尝试用间接荧光抗体法来快速诊断该病。采用特异性抗 J亚群禽白血病病毒囊膜蛋白 gp85单克隆抗体对组织触片作间接荧光抗体试验 ,检测 J亚群禽白血病病毒抗原。在骨髓、食管、肌肉、输卵管、肾都出现了范围广而且很明亮的荧光 ;肝、肺、脾的荧光较为明亮 ;心脏的荧光较弱 ,但清晰可见。表明在病料组织中存在特异性病毒抗原。 | 徐镔蕊 董卫星 何召庆 冯小宇 余春明 张丽 任艳丽 L F LEE Mao-xiang LI | 2002 | 中国兽医杂志2002，38，9： | 49 |
| 2 | 柑桔黄龙病的鉴定和柯赫氏定理(英文)显示文摘黄龙(梢)病被认为是世界柑橘生产上的毁灭性病害已超过一个世纪,但该病害的病原学至今还没有清晰地建立起来。根据16S rRNA基因序列分析,一组被称为'Candidatus Liberibacter species'的难培养细菌被认为与黄龙病相关。然而,要确定'Ca.Liberibacter spp.'是黄龙病病原的柯赫氏定理并没有真正完成。令我们担忧的是,近年来有些文献频频指出'Ca.Liberibacter spp.'是黄龙病的病原,其实该细菌与柑橘寄主的直接病理反应还没有完全确定。我们建议,在黄龙病病原学清楚之前,文献报道在这方面需要有准确的阐述。 | Chen J Deng X Civerolo E L Lee R F Jones J B Zhou C Hartung J S Manjunath K L Brlansky R H | 2011 | 植物病理学报2011，41，2： | 10 |
| 3 | Increased catabolism and decreased unsaturation of ganglioside in patients with inflammatory bowel disease显示文摘AIM: To investigate whether accelerated catabolism of ganglioside and decreased ganglioside content contribute to the etiology of pro-inflammatory intestinal disease. METHODS: Intestinal mucosa from terminal ileum or colon was obtained from patients with ulcerative colitis or inflammatory Crohn's disease(n = 11) undergoing bowel resection and compared to control samples of normal intestine from patients with benign colon polyps(n = 6) and colorectal cancer(n = 12) in this observational case-control study. Gangliosides and phospholipids of intestinal mucosa were characterized by class and ceramide or fatty acid composition using liquid chromatography triple-quad mass spectrometry. Content and composition of ganglioside classes GM1, GM3, GD3, GD1 a, GT1 and GT3 were compared among subject groups. Content and composition of phospholipid classes phosphatidylcholine(PC) and phosphatidylethanolamine were compared among subject groups. Unsaturation index of individual ganglioside and phospholipid classes was computed and compared among subject groups. Ganglioside catabolism enzymes beta-hexosaminidase A(HEXA) and sialidase-3(NEU3) were measured in intestinal mucosa using western blot and compared among subject groups. RESULTS: Relative GM3 ganglioside content was 2-fold higher(P < 0.05) in intestine from patients with inflammatory bowel disease(IBD) compared to control intestine. The quantity of GM3 and ratio of GM3/GD3 was also higher in IBD intestine than control tissue(P < 0.05). Control intestine exhibited 3-fold higher(P < 0.01) relative GD1 a ganglioside content than IBD intestine. GD3 and GD1 a species of ganglioside containing three unsaturated bonds were present in control intestine, but were not detected in IBD intestine. The relative content of PC containing more than two unsaturated bonds was 30% lower in IBD intestine than control intestine(P < 0.05). The relative content of HEXA in IBD intestine was increased 1.7-fold(P < 0.05) and NEU3 was increased 8.3-fold(P < 0.01) compared to normal intestine. Intestinal mucosa in IBD is characterized by increased GM3 content, decreased GD1 a, and a reduction in polyunsaturated fatty acid constituents in GD3, GD1 a and PC.CONCLUSION: This study suggests a new paradigm by proposing that IBD occurs as a consequence of increased metabolism of specific gangliosides. | John J Miklavcic Glen K Shoemaker Vera C Mazurak M Tom Clandinin Tasha DL Hart Kareena L Schnabl Gordon M Lees Bodil MK Larsen Oliver F Bathe Alan BR Thomson M Tom Clandinin | 2015 | World Journal of Gastroenterology2015，21，35： | 3 |
| 4 | 用萤光抗体试验筛选能表达鸡马立克病病毒pp38基因的重组昆虫杆状病毒显示文摘用抗鸡马立克病病毒(MDV)的38Kd磷蛋白的单克隆抗体作萤光抗体试验,直接从用可转染性野生型杆状病毒AcMNPV株DNA与含MDVpp38基因的重组转基因质粒载体DNA共同转染的Sf 9单层细胞培养中筛选到能表达pp38基因产物的重组杆状病毒感染斑,并从保存于琼脂醣凝胶上的复印斑中收复并进一步克隆了该基因重组病毒.该法易于实施,即使未能熟练掌握根据杆状病毒多角体来区别野生型杆状病毒斑与基因重组病毒斑的技术,也可顺利地筛选到能表达目的基因的重组合病毒。 | 崔治中 Lee L F | 1992 | 江苏农学院学报1992，13，1： | 2 |
| 5 | Glial fibrillary acidic protein: GFAP-thirty-one years (1969-2000)显示文摘 | Eng L F Ghirnikar R S Lee Y L | 2000 | Neurochem Res2000，25，910： | 2 |
| 6 | 卒中介入治疗培训指南:国际多学会共识文件显示文摘1背景
缺血性卒中是全球人口死亡和残疾的首要原因。很多急性大血管闭塞(emergent large vesselocclusion,ELVO)患者都会遗留长期残疾。事实上,这些颅内大动脉闭塞经常会导致大面积脑损伤,进而造成患者死亡或严重致残。 | Lavine SD Cockroft K Hoh B Bambakidis N Khalessi AA Woo H Riina H Siddiqui A Hirsch JA Chong W Rice H Wenderoth J Mitchell P Coulthard A Signh TJ Phatorous C Khangure M Klurfan P ter Brugge K Iancu D Gunnarsson T Pongpech S Rodesch G Soderman M Taylor A Krings T Orbach D Picard L Suh DC Zheng HQ Jansen O Muto M Szikora I Pierot L Brouwer P Gralla J Renowden S Andersson T Fiehler J Turjman F White P Januel AC Spelle L Kulcsar Z Chapot R Biondi A Dima S Taschner C Szajner M Krajina A Sakai N Matsumaru Y Yoshknura S Ezura M Fujinaka T Iihara K Ishii A Higashi T Hirohata M Hyodo A Ito Y Kawanishi M Kiyosue H Kobayashi E Kobayashi S Kuwayama N Matsumoto Y Miyachi S Murayama Y Nagata I Nakahara I Nemoto S Niimi Y Oishi H Satomi J Satow T Sugiu K Tanaka M Terada T Yamagami H Diaz O Lylyk P Jayaraman MV Patsalides A Gandhi CD Lee SK Abruzzo T Albani B Ansari SA Arthur AS Baxter BW Bulsara KR Chen M Almandoz JE Fraser JF Heck DV Hetts SW Hussain MS Klucznik RP Leslie-Mawzi TM Mack WJ McTaggart RA Meyers PM Mocco J Prestigiacomo CA Pride GL Rasmussen PA Starke RM Sunenshine PJ Tarr RW Frei DF Pabo M Nogueira RG Zaidat OO Jovin T Linfante I Yavagal D Liebeskind D Novakovic R Pongpech S 许岩 孙瑞 郭芮兵 | 2017 | 国际脑血管病杂志2017，25，5： | 2 |
| 7 | 端粒和端粒酶与肿瘤关系的研究进展显示文摘端粒是末端染色体 ,维持染色体的稳定。端粒酶是染色体末端转移酶 ,是合成端粒DNA的酶 ,对端粒的结构起着稳定的作用。端粒、端粒酶的研究始于 2 0世纪初期 ,但直到近几年科研人员才发现他们与肿瘤有着密切的关系。在人类端粒酶的研究过程中发现端粒酶在约 85 %以上的恶性肿瘤中呈阳性 ,而动物肿瘤与端粒酶的研究还是空白。文章主要介绍端粒、端粒酶的结构功能及它们与肿瘤关系的研究进展 ,并讨论世界上首例蛋鸡 J亚群禽白血病的自然发病的病例的端粒酶 TERT表达。作者曾用辣根过氧化物酶标记的链酶卵白素进行免疫组化检测 ,结果在病鸡的心、肝、脾、肺、肾、气管、十二指肠、睾丸、骨髓、脑、胸腺、法氏囊等组织中发现 TERT表达呈阳性 ,说明在蛋鸡 J亚群禽白血病发生时端粒酶活性升高 ,提示端粒酶参与了该病的发生发展机制 。 | 徐镔蕊 冯小宇 董卫星 余春明 李宁 李博 Lee L F LI Mao-xiang | 2003 | 动物医学进展2003，24，3： | 2 |
| 8 | Inhibitory effect of luteolin on hepatocyte growth factor/ scatter factor-induced HepG2 cell invasion involving both MAPK/ERKs and PI3K-Akt pathways 显示文摘 | LEE W J WU L F CHEN W K | 2006 | Chem Biol Interact2006，160，2： | 1 |
| 9 | Preparation and Physical Prop- erties of Hollow Glass Microspheres Reinforced Epoxy Matrix Resins显示文摘 | Park S J Jin F L Lee C | 2005 | Materials Science and Engineering A2005，402，2： | 1 |
| 10 | Tape-Arabidopsis Sandwich - a simpler Arabidopsis protoplast isolation method 显示文摘 | Wu F H Shen S C Lee L Y | 2009 | Plant Methods2009，5，16： | 1 |
| 11 | A prospective randomized controlled trial on ureteral stenting after ureteroscopic holmium laser lithotripsy显示文摘 | CHEUNG M C LEE F LEUNG Y L | 2003 | J Urol2003，169，4： | 1 |
| 12 | Immobilization of Wholecell penicillin G acylase by entrapping within polymethacrylamide beads显示文摘 | Hsiau L T Lee W C Wang F S | 1997 | Appl Biochem Biotechnol1997，62，： | 1 |
| 13 | Estimation of Spatial Autoregressive Panel Data Model with Fixed Effects 显示文摘 | Lee L F Yu J | 2010 | Journal of Econometrics2010，154，2： | 1 |
| 14 | Virtual Community Informatics: A Review and Research Agenda显示文摘 | Lee F S L Vogel D Limayem M | 2003 | Journal of Information Technology and Applica- tion2003，5，1： | 1 |
| 15 | Plastic-damage model for cyclic loading of concrete structures 显示文摘 | Lee J Gregory L F | 1998 | Journal of Engineer- ingMechanics1998，124，4： | 1 |
| 16 | Infectious bill atrophy syndrome caused by parvovirus in a co-outbreak with duck viral hepatitis in duckings in Taiwan 显示文摘 | Lu Y S Lin D F Lee Y L | 1993 | Avian Diseases1993，37，： | 1 |
| 17 | Semiparametric estimation of nonlinear error-in-variable models with validation study显示文摘 | Sepanski J H Lee L F | 1995 | J Nonparametr Statist 41995，4，4： | 1 |
| 18 | The measurement and sources of technical inefficiency in the indonesian weaving industry 显示文摘 | Pitt M and Lee L F | 1981 | Journal of Development Economics1981，9，1： | 1 |
| 19 | Penetration failure of spectra polyethylene fiber-reinforced ballistic-grade composites显示文摘 | Walsh T F Lee B L Song J W | 1998 | Key Engineering Materials1998，，141143： | 1 |
| 20 | Factors affecting risk of symptomatic temporal lobe necrosis:significance of fractional dose and treatment time显示文摘 | Lee A W KWong D L Leung S F | 2002 | Int J Radiat Oneol Biol Phys2002，53，1： | 1 |
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