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| 1 | Screening Helicobacter pylori genes induced during infection of mouse stomachs显示文摘AIM:To investigate the effect of in vivo environment on gene expression in Helicobacter pylori(H.pylori) as it relates to its survival in the host.METHODS:In vivo expression technology(IVET) systems are used to identify microbial virulence genes.We modified the IVET-transcriptional fusion vector,pIVET8,which uses antibiotic resistance as the basis for selection of candidate genes in host tissues to develop two unique IVET-promoter-screening vectors,pIVET11 and pIVET12.Our novel IVET systems were developed by the fusion of random Sau3A DNA fragments of H.pylori and a tandem-reporter system of chloramphenicol acetyltransferase and beta-galactosidase.Additionally,each vector contains a kanamycin resistance gene.We used a mouse macrophage cell line,RAW 264.7 and mice,as selective media to identify specific genes that H.pylori expresses in vivo.Gene expression studies were conducted by infecting RAW 264.7 cells with H.pylori.This was followed by real time polymerase chain reaction(PCR) analysis to determine the relative expression levels of in vivo induced genes.RESULTS:In this study,we have identified 31 in vivo induced(ivi) genes in the initial screens.These 31 genes belong to several functional gene families,including several well-known virulence factors that are expressed by the bacterium in infected mouse stomachs.Virulence factors,vacA and cagA,were found in this screen and are known to play important roles in H.pylori infection,colonization and pathogenesis.Their detection validates the efficacy of these screening systems.Some of the identified ivi genes have already been implicated to play an important role in the pathogenesis of H.pylori and other bacterial pathogens such as Escherichia coli and Vibrio cholerae.Transcription profiles of allivi genes were confirmed by real time PCR analysis of H.pylori RNA isolated from H.pylori infected RAW 264.7 macrophages.We compared the expression profile of H.pylori and RAW 264.7 coculture with that of H.pylori only.Some genes such as cag A,vac A,lpx C,mur I,tlp C,trx B,sod B,tnp B,pgi,rbf A and inf B showed a 2-20 fold upregulation.Statistically significant upregulation was obtained for all the above mentioned genes(P < 0.05).tlp C,cag A,vac A,sod B,rbf A,inf B,tnp B,lpx C and mur I were also significantly upregulated(P < 0.01).These data suggest a strong correlation between results obtained in vitro in the macrophage cell line and in the intact animal.CONCLUSION:The positive identification of these genes demonstrates that our IVET systems are powerful tools for studying H.pylori gene expression in the host environment. | Aparna Singh Nathaniel Hodgson Ming Yan Jungsoo Joo Lei Gu Hong Sang Emmalena Gregory-Bryson William G Wood Yisheng Ni Kimberly Smith Sharon H Jackson William G Coleman | 2012 | World Journal of Gastroenterology2012，18，32： | 5 |
| 2 | 鱼、蛋、牛奶、坚果、蔬菜和水果摄入量大,以及低盐膳食模式与高血压的患病率和控制率相关显示文摘食物对高血压患病率和控制率的影响尚不清晰。本文旨在中国人群中调查鱼、蛋、牛奶、坚果、蔬菜和水果摄入量大以及低盐膳食模式是否与高血压有关。方法:从2012年9月到2014年12月共招募15303名受试者进行病例对照研究,分为高血压组(n=1604)和正常血压组(n=13660),其中高血压组又分为血压控制亚组(n=397)和血压未控制亚组(n=1207)。收集过去1 年里每周平均鱼、蛋、牛奶、坚果、蔬菜、水果和盐摄入量数据。鱼、蛋、牛奶、坚果、蔬菜、水果和盐的平均摄入量分别为117、150、35、20、2100、300和58g/周。 | Yu G Fu H Huang W Zhang N Deng D Li G Lei H | 2019 | 中华高血压杂志2019，27，5： | 2 |
| 3 | Synthesis-Structure-Performance Relationship of Cocondensed Phenol-Urea-Formaldehyde Resins by MALD1-ToF andl3CNMR显示文摘 | Du G B Lei H Pizzi A | 2008 | JAppIPolymSci2008，110，2： | 1 |
| 4 | N-acetylcysteine suppresses retinal detachment in an experimen-tal model of proliferative vitreoretinopathy显示文摘 | Lei H Velez G Cui J | 2010 | Am J Pathol2010，177，1： | 1 |
| 5 | Performance of long rectangular barrettes in granitic saprolites 显示文摘 | Ng C W W Lei G H | 2003 | Journal of Geotechnical and Geoenvironmental Engineering America Society of Civil Engineers2003，129，8： | 1 |
| 6 | Non-invasive quantitative assessment of oxidative metabolism in quadriceps muscles by near infrared spec- troscopy 显示文摘 | Ding H Wang G Lei W | 2001 | Br J Sports Med2001，35，6： | 1 |
| 7 | Wear and corrosion resistance of A1 ion implanted AZ31 magnesium alloy 显示文摘 | LEI M K LI P YANG H G | 2007 | Surface & Coat ings Technology2007，201，： | 1 |
| 8 | Mathematical heat transfer model research for the improvement of continuous casting slab temperature显示文摘 | Wang H Li G Lei Y | 2005 | ISIJ Int2005，45，9： | 1 |
| 9 | Cross-linked soy-based wood ad- hesives for plywood 显示文摘 | Lei H Wu Z Du G | 2014 | hernational Journal of Adhesion & Ad- hesives2014，50，： | 1 |
| 10 | An analytical solution for consolidation with vertical drains under multi-romp loading显示文摘 | LEI G H ZHENG Q NG C W W | 2015 | G6otechnique2015，65，7： | 1 |
| 11 | Discussion on consolidation test and some relative problems显示文摘 | SHI J Y WEN Y X LEI G H | 2004 | Journal of Hohai University:Natural Science2004，32，2： | 1 |
| 12 | The growth of MgO buffer layers on sapphire for the epitaxy of BaTiO3 optical thin film 显示文摘 | LISONI J G SIEGERT M LEI C H | 2001 | Thin Solid Films2001，389，12： | 1 |
| 13 | The Ag-BiOBrx composite photocatalyst;preparation,character-ization and their novel pollutants removal property显示文摘 | Lei Y Q Wang G H Guo P R | 2013 | Applied Surface Science2013，279，： | 1 |
| 14 | 查看详情显示文摘 | Yan L W Duan X R Ding X T Dong J Q Yang Q W Liu Y Zou X L Liu D Q Xuan W M Chen L Y Rao J Song X M Huang Y Mao W C Wang Q M Li Q Cao Z Li B Cao J Y Lei G J Zhang J H Li X D Chen W Cheng J Cui C H Cui Z Y Deng Z C Dong Y B Feng B B Gao Q D Han X Y Hong W Y | | 0，，： | 1 |
| 15 | Low-temperature NH3-SCR of NO by lanthanum manganite perovskites: Effect of A-/B-site substitution and TiO2/CeO2 support显示文摘 | ZHANG R D YANG W LUO N LI P LEI Z G CHEN B H | 2014 | Appl Catal B: Environ2014，146，： | 1 |
| 16 | An approximate ana- lytical solution for calculating ground surface settle- ments due to diaphragm walling 显示文摘 | Lei G H Sun H S Ng C W W | 2014 | Computers and Geotechnics2014，61，： | 1 |
| 17 | 显示文摘 | Lei Y Z Zhao G H Liu M C | 2009 | Phys Chem C2009，113，19： | 1 |
| 18 | The interpretation of pendulum-type inclinometer readings显示文摘 | LEI G H AI Y B SHI J Y | 2006 | Canadian Geotechnical Journal2006，43，2： | 1 |
| 19 | Influence of de electric current on the hardness of thermally aged Cu-Cr-Zr alloy 显示文摘 | Wang Z J Zhong Y B Cao G H Wang C Wang J Ren W L Lei Z S Ben Z M | 2009 | Journal of Alloys and Compounds2009，479，： | 1 |
| 20 | Cloning and phylogenetic a- nalysis of the NSP1 - 5 genes of giant panda mtavirus strain CH - 1 isolated in China显示文摘 | LEI Y YAN Q G ZHANG Z H | 2011 | Afr J Microbiol Res2011，5，31： | 1 |
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