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| 1 | The complete reference genome for grapevine (Vitis vinifera L.) genetics and breeding显示文摘Grapevine is one of the most economically important crops worldwide.However,the previous versions of the grapevine reference genome tipically consist of thousands of fragments with missing centromeres and telomeres,limiting the accessibility of the repetitive sequences,the centromeric and telomeric regions,and the study of inheritance of important agronomic traits in these regions.Here,we assembled a telomere-to-telomere(T2T)gap-free reference genome for the cultivar PN40024 using PacBio HiFi long reads.The T2T reference genome(PN_T2T)is 69 Mb longer with 9018 more genes identified than the 12X.v0 version.We annotated 67%repetitive sequences,19 centromeres and 36 telomeres,and incorporated gene annotations of previous versions into the PN_T2T assembly.We detected a total of 377 gene clusters,which showed associations with complex traits,such as aroma and disease resistance.Even though PN40024 derives from nine generations of selfing,we still found nine genomic hotspots of heterozygous sites associated with biological processes,such as the oxidation–reduction process and protein phosphorylation.The fully annotated complete reference genome therefore constitutes an important resource for grapevine genetic studies and breeding programs. | Xiaoya Shi Shuo Cao Xu Wang Siyang Huang Yue Wang Zhongjie Liu Wenwen Liu Xiangpeng Leng Yanling Peng Nan Wang Yiwen Wang Zhiyao Ma Xiaodong Xu Fan Zhang Hui Xue Haixia Zhong Yi Wang Kekun Zhang Amandine Velt Komlan Avia Daniela Holtgräwe Jérôme Grimplet JoséTomás Matus Doreen Ware Xinyu Wu Haibo Wang Chonghuai Liu Yuling Fang Camille Rustenholz Zongming Cheng Hua Xiao Yongfeng Zhou | 2023 | Horticulture Research2023，10，5： | 3 |
| 2 | A mesoporous silica supported Hg^2+ chemodosimeter显示文摘 | Leng B Jiang J Tian H | 2010 | Aiche Journal2010，56，11： | 1 |
| 3 | Heteropolyanion basedionic liquids:reaction-induced self-separation catalystsfor esterification显示文摘 | Leng Y Wang J Zhu D R | 2009 | Angew Chem Int Ed2009，48，1： | 1 |
| 4 | Study of the zinc electroplating process using electrochemical noise technique 显示文摘 | ZHANG Z LENG W H CAI Q Y CAO F H ZHANG J Q | 2005 | Journal of Electroanalytical Chemistry2005，578，： | 1 |
| 5 | Construction of bivariate nonseparable compactly supported orthogonal wavelets显示文摘 | LENG J S HUANG T Z CATTANI C | 2013 | Math Problems in Engineering2013，2013，1： | 1 |
| 6 | The apoptotic effect and associated signalling of HSP90 inhibitor 17-DMAG in hepatocellular carcinoma cells显示文摘 | Leng AM Liu T Yang J | 2012 | Cell Biol Int2012，36，10： | 1 |
| 7 | Cyclooxygenase-2 promotes hepatocelluar carcinoma cell growth throush Akt activation:evidence for Akt inhibition in celecoxib-induced apoptosis显示文摘 | Han C Demetris AJ | 2003 | Hepatology2003，38，3： | 1 |
| 8 | Macrophage migration inhibitory factor stimulates AMP-activated protein kinase in the ischaemic heart显示文摘 | Li J Leng L | 2008 | Nature2008，451，7178： | 1 |
| 9 | Geometrical nonlinear aeroelastic stability analysis of a composite high-aspect-ratio wing显示文摘 | Xie C C Leng J Z Yang C | 2008 | Shock and Vibration2008，15，32： | 1 |
| 10 | Cyclooxygenase-2 promotes hepatocellular carcinoma cell growth through Akt activation:evidence for Akt inhibition in celecoxib-induced apoptosis显示文摘 | Leng J Han C Demetris AJ | 2003 | Hepatology2003，38，3： | 1 |
| 11 | 显示文摘 | Leng Y J Chan S H Khor K A | 2004 | International Journal of Hydrogen Energy2004，，29： | 1 |
| 12 | The microstructure and mechanical properities of TiN and TiO2/TiN duplex films synthesized synthesized by plasma immersion ion implantlon and deposition on artificial valve 显示文摘 | Leng Y X Chen J Y Yang P | 2006 | Surface & Coating Technology2006，201，： | 1 |
| 13 | Macrophage migration inhibitory factor stimulates AMP-activated protein kinase in the ischaemic heart 显示文摘 | Miller E J Li J Leng L | 2008 | Nature2008，451，7178： | 1 |
| 14 | Heteropolyanion-based i- onic hybrid solid:A green bulk-type catalyst for hydroxyl- ation of benzene with hydrogen peroxide 显示文摘 | Leng Y Wang J Zhu D R | 2011 | Chem Eng J2011，173，2： | 1 |
| 15 | A method for constructing trivariate nonseparable compactly supported orthogonal wavelets显示文摘 | LENG J S HUANG T Z FU Y D | 2009 | Inter J Comput Math2009，86，7： | 1 |
| 16 | TLR3signaling in macrophages is indispensable for the protective immunity of invariant natural killer T cells against enterovirus 71infection显示文摘 | Zhu K Yang J Luo K Yang C Zhang N Xu R Chen J Jin M Xu B Guo N Wang J Chen Z Cui Y Zhao H Wang Y Deng C Bai L Ge B Qin C F Shen H Yang C F Leng Q | 2015 | PLoS Pathog2015，11，10： | 1 |
| 17 | Reductive debromination of polybrominated diphenyl ethers in anaerobic sediment and a biomimetic system显示文摘 | TOKARZ Ⅲ J A AHN M Y LENG J | 2008 | Environmental Science & Technology2008，42，4： | 1 |
| 18 | The cyclooxygenase-2 inhibitor celecoxib blocks phosphorylation of Akt and induces apoptosis in human cholangiocarcinoma cells 显示文摘 | Wu T Leng J Han C | 2004 | Mol Cancer Ther2004，3，3： | 1 |
| 19 | ErbB2 is necessary for induction of carcinoma cell invasion by ErbB family receptor tyrosine kinases 显示文摘 | Spencer KS Graus-Porta D Leng J | 2000 | J Cell Biol2000，148，： | 1 |
| 20 | Speckle Suppression in Dig- ital Holographic Imaging with Random Phases and Differ- ent Wavelengths显示文摘 | LENG J SANG X YAN B | 2014 | Optical Engineering2014，53，03： | 1 |
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