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| 1 | Role of the Arabidopsis thafiana NAC transcription factors ANAC019 and ANAC055 in regulating jasmonic acid-signaled defense responses显示文摘Jasmonic 酸(JA ) 是对草食动物攻击,病原体感染并且机械伤害调整植物防卫回答的重要植物激素。在这份报告,我们提供了生物化学、基因的证据证明 Arabidopsis thaliana NAC 家庭蛋白质 ANAC019 和 ANAC055 可能作为抄写使活跃之物工作调整辩护基因的导致 JA 的表示。在 JA 发信号的二 NAC 基因的角色与 anac019 anac055 被检验两倍异种并且与转基因的植物 overexpressing ANAC019 或 ANAC055。两倍变异的植物显示出的 anac019 anac055 稀释了导致 JA 的植物的存储 PROTEIN1 (VSP1 ) 和 LIPOXYGENASE2 (LOX2 ) 表示,而二 NAC 基因显示出的转基因的植物 overexpressing 提高了导致 JA 的 VSP1 和 LOX2 表示。二 NAC 基因的 导致JA 的表示取决于 COI1 和 AtMYC2 的功能和发现 ANAC019 的那 overexpression 部分救了 atmyc2-2 异种的JA相关的显型,带了我们到二 NAC 蛋白质 AtMYC2 下游地扮演调整 发信号JA 的防卫回答的一个假设。证实这个想法的进一步的证据来自观察到 necrotrophic 真菌的两倍异种高显示出的 anac019 anac055 的反应类似到 atmyc2-2 异种的。 | Qingyun Bu Hongling Jiang Chang-Bao Li Qingzhe Zhai Jie Zhang Xiaoyan Wu Jiaqiang Sun Qi Xie Chuanyou Li | 2008 | Cell Research2008，18，7： | 60 |
| 2 | Energy balance closure at ChinaFLUX sites显示文摘Network of eddy covariance observation is measuring long-term carbon and water fluxes in contrasting ecosystems and climates. As one important reference of independently evaluating scalar flux estimates from eddy covariance, energy balance closure is used widely in study of carbon and water fluxes. Energy balance closure in ChinaFLUX was evaluated by statistical regression of turbulent energy fluxes (sensible and latent heat) against available energy (net radiation, soil heat flux, canopy heat storage) and the energy balance ratio (EBR) and the frequency distribution of relative errors of energy balance (δ). The trends of diurnal and seasonal variation of energy balance in ChinaFLUX were analyzed. The results indicated that the imbalance was prevalent in all observation sites, but there were little differences among sites because of the properties variation of sites. The imbalance was greater during nocturnal periods than daytime and closure was improved with friction velocity intensifying. Generally the results suggested that estimates of the scalar turbulent fluxes of sensible and latent heat were underestimated and/or that available energy was overestimated. Finally, we discussed certain factors that are contributed to the imbalance of energy, such as systematic errors associated with the sampling mismatch, systematic instrument bias, neglected energy sinks, low and high frequency loss of turbulent fluxes and advection of heat and water vapor. | LI Zhengquan, YU Guirui, WEN Xuefa, ZHANG Leiming, REN Chuanyou & FU Yuling Chinese Ecosystem Research Network Synthesis Research Center, Institute of Geographic Sciences and Natural Resources Research, Chinese Academy of Sciences, Beijing 100101, China Graduate School of the Chinese Academy of Sciences, Beijing 100039, China | 2005 | Science China Earth Sciences2005，48，z1： | 50 |
| 3 | Lushan M_S7.0 earthquake:A blind reserve-fault event显示文摘In the epicenter of the Lushan MS7.0 earthquake there are several imbricate active reverse faults lying from northwest to southeast,namely the Gengda-Longdong,Yanjing-Wulong,Shuangshi-Dachuan and Dayi faults.Emergency field investigations have indicated that no apparent earthquake surface rupture zones were located along these active faults or their adjacent areas.Only brittle compressive ruptures in the cement-covered pavements can be seen in Shuangshi,Taiping,Longxing and Longmen Townships,and these ruptures show that a local crustal shortening occurred in the region during the earthquake.Combining spatial distribution of the relocated aftershocks and focal mechanism solutions,it is inferred that the Lushan earthquake is classified as a typical blind reverse-fault earthquake,and it is advised that the relevant departments should pay great attention to other historically un-ruptured segments along the Longmenshan thrust belt and throughout its adjacent areas. | XU XiWei WEN XueZe HAN ZhuJun CHEN GuiHua LI ChuanYou ZHENG WenJun ZHNAG ShiMin REN ZhiQun XU Chong TAN XiBin WEI ZhanYu WANG MingMing REN JunJie HE ZhongTai LIANG MingJian | 2013 | Chinese Science Bulletin2013，58，28： | 35 |
| 4 | Late Cenozoic deformation subsequence in northeastern margin of Tibet --Detrital AFT records from Linxia Basin显示文摘Two events of Tibet uplifting are revealed by detrital apatite fission track (AFT) age data from Linxia Basin. They occurred at about 14 and 5.4-8.0 MaBP respectively. We interpret the first one to be related to the uplifting of the northern Tibet, which might have resulted from convectively removing the thickened lower lithosphere. The second one is a result of Laji Mountain uplifting. Numerous studies of the Tibetan Plateau suggest that the onset time of the deformation in the northeastern margin of Tibetan Plateau and the time of Tibet attaining to its present elevation is about 8 MaBP. They are approximately coincident with the uplift of Lajishan Mountain. It suggests that the northeastern margin of Tibet propagated northeastwardly to its present site in about 8 MaBP for accommodating the sustained convergence between India-Eurasia plate and for keeping its high elevation. The active block pattern dominating the strong earthquake distribution of Chinese continent probably formed at about 8.0-5.4 MaBP. | ZHENG Dewen (郑德文) ZHANG Peizhen (张培震) WAN Jinglin (万景林) LI Chuanyou (李传友) CAO Jixiu (曹继秀) | 2003 | Science China Earth Sciences2003，46，z2： | 25 |
| 5 | RGF1 INSENSITIVE 1 to 5, a group of LRR receptor-like kinases, are essential for the perception of root meristem growth factor I in Arabidopsis thaliana显示文摘RGF1,分泌的肽荷尔蒙,戏在 Arabidopsis 在根分裂组织开发给角色调音。以前的研究显示功能的 RGF1 需要是在由 tyrosylprotein sulfotransferase 的酷氨酸残余的 sulfated 并且 RGF1 主要经由二个下游的抄写因素调整根分裂组织活动,过多 1 (PLT1 ) 并且 PLT2。然而,细胞外的 RGF1 怎么被一个植物房间察觉,是不清楚的。用基因途径,我们发现了充满白氨酸的重复的 clade 像受体的 kinases,指定了为 RGF1 感觉迟钝 1 (RGI1 ) 到 RGI5,用作 RGF1 的受体。二独立 rgi1 rgi2 rgi3 rgi4 rgi5 五倍的异种与分裂组织的一种小尺寸显示一致的短主要的根显型。四倍的异种显示出的 rgi1 rgi2 rgi3 rgi4 到 RGF1 的显著地减少的敏感,和五倍的异种对 RGF1 完全感觉迟钝。PLT1 和 PLT2 的表示在五倍的异种是几乎无法发现的。一个 RGI2 倡导者极大地在五倍的异种驾驶的 PLT2 的宫外的表示救了它的根分裂组织缺点。RGI 之一, RGI1,随后详细生物化学地被分析。在 vitro 点弄污和下拉,分析显示 RGI1 能身体上与 RGF1 交往。RGF1 的外长的申请能快速并且同时导致 RGI1 的 phosphorylation 和 ubiquitination,显示 RGI1 能察觉并且 transduce RGF1 肽信号。然而,激活的 RGI1 多半很快被翻。这些结果表明那 RGI,充当 RGF1 的受体,在在 Arabidopsis thaliana 的 RGF1-PLT-mediated 根分裂组织开发的戏必需品角色。 | Yang Ou Xiaoting Lu Quaner Zi Qingqing Xun Jingjie Zhang Yujun Wu Hongyong Shi Zhuoyun Wei Baolin Zhao Xiaoyue Zhang Kai He Xiaoping Gou Chuanyou Li Jia Li | 2016 | Cell Research2016，26，6： | 18 |
| 6 | A Transcriptional Network Promotes Anthocyanin Biosynthesis in Tomato Flesh显示文摘Dietary anthocyanins are important health-promoting antioxidants that make a major contribution to the quality of fruits. It is intriguing that most tomato cultivars do not produce anthocyanins in fruit. However, the purple tomato variety Indigo Rose, which has the dominant Aft locus combined with the recessive atv locus from wild tomato species, exhibits light-dependent anthocyanin accumulation in the fruit skin. Here, we report that Aft encodes a functional anthocyanin activator named SlAN2-like, while atv encodes a nonfunctional version of the anthocyanin repressor SlMYBATV. The expression of SlAN2-like is responsive to light, and the functional SlAN2-like can activate the expression of both anthocyanin biosynthetic genes and their regulatory genes, suggesting that SlAN2-like acts as a master regulator in the activation of anthocyanin biosynthesis. We further showed that cultivated tomatoes contain nonfunctional alleles of SlAN2-like and therefore fail to produce anthocyanins. Consistently, expression of a functional SlAN2-like gene driven by the fruit-specific promoter in a tomato cultivar led to the activation of the entire anthocyanin biosynthesis pathway and high-level accumulation of anthocyanins in both the peel and flesh. Taken together, our study exemplifies that efficient engineering of complex metabolic pathways could be achieved through tissue-specific expression of master transcriptional regulators. | Chuanlong Sun Lei Deng Minmin Du Jiuhai Zhao Qian Chen Tingting Huang Hongling Jiang Chang-Bao Li Chuanyou Li | 2020 | Molecular Plant2020，13，1： | 17 |
| 7 | Seasonal patterns and environmental control of ecosystem respiration in subtropical and temperate forests in China显示文摘Continuous measurement of carbon dioxide exchange using the eddy covariance (EC) technique was made at two ChinaFLUX forest sites including the young subtropical Pinus plantation (Qianyanzhou) and old temperate broad-leaved Korean pine mixed forest (Changbai Mountains) as part of the ChinaFLUX network. Seasonal patterns and environmental control of ecosystem respiration in the subtropical and temperate forests were evaluated by the often-used multiplicative model and Q10 model as a function of temperature and soil water content. The resuits suggested that ( i ) temperature was found to be a dominant factor in the ecosystem respiration, and most of the temporal variability of ecosystem respiration was explained by temperature. However, in the drought-stressed ecosystem, soil water content controlled the temporal variability of ecosystem respiration other than temperature effects, and soil water content became a dominat factor when severe drought affected the ecosystem respiration; (ii) the regression models analysis revealed that in the drier soil, ecosystem respiration was more sensitive to soil moisture than was expressed by the often-used multiplicative model. It was possible to accurately estimate the seasonal variation of ecosystem respiration based on the Q10 model; and (iii)annual ecosystem respiration derived from the often-used multiplicative model was 1209 g C m-2and 1303 g C m-2, and was consistently a little higher than the Q10 model estimates of 1197 g C m-2 and 1268 g C m-2 for Qianyanzhou and Changbai Mountains, respectively. | YU Guirui, WEN Xuefa, LI Qingkang, ZHANG Leiming, REN Chuanyou, LIU Yunfen & GUAN Dexin Chinese Ecosystem Research Network Synthesis Research Center, Institute of Geographic Sciences and Natural Resources Research, Chinese Academy of Sciences, Beijing 100101, China Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang 110016, China Graduate School of the Chinese Academy of Sciences, Beijing 100039, China | 2005 | Science China Earth Sciences2005，48，z1： | 13 |
| 8 | Efficient generation of pink-fruited tomatoes using CRISPR/Cas9 system显示文摘Tomato(Solanum lycopersicum)is the leading vegetable crop worldwide and an essential component of a healthy diet(Lin et al.,2014;Du et al.,2017).Fruit color is regarded as one of the most important commercial traits in tomato(The Tomato Genome Consortium,2012).Consumers in different regions have different color preferences.For example,European and | Lei Deng Hang Wang Chuanlong Sun Qian Li Hongling Jiang Minmin Du Chang-Bao Li Chuanyou Li | 2018 | Journal of Genetics and Genomics2018，45，1： | 13 |
| 9 | The Arabidopsis P450 protein CYP82C2 modulates jasmonateinduced root growth inhibition, defense gene expression and indole glucosinolate biosynthesis显示文摘Jasmonic 酸(JA ) 是对草食动物和一些微生物引起的病原体调整大量植物防卫回答的一个丰满的导出酸的发信号分子。分子的基因研究证实了 JA 也在植物开发的几个方面执行一个关键角色。这里,我们描述 Arabidopsis 变异的 jasmonic acid-hypersensitive1-1 (jah1-1 ) 的描述,它在 JA 回答的几个方面是有缺点的。尽管异种在根生长抑制展出增加的敏感到 JA,它显示出 JA 可诱导的防卫基因和减少的抵抗的减少的表示到 necrotrophic 真菌 Botrytis cinerea。基因克隆研究显示这些缺点被一个变化在细胞色素 P450 蛋白质 CYP82C2 引起。我们提供证明的证据到 B 的 jah1-1 异种的损害抵抗。cinerea 被导致 JA 的防卫基因的减少的表示伴随并且减少了导致 JA 的吲哚 glucosinolates (IG ) 的累积。相反地,提高的抵抗到 B。在 CYP82C2-overexpressing 植物的 cinerea 被导致 JA 的防卫基因的增加的表示伴随并且提高了导致 JA 的 IG 的层次。我们证明 CYP82C2 影响 IG biosynthetic 先锋的导致 JA 的累积色氨酸(Trp ) ,然而并非导致 JA 的 IAA 或导致病原体的 camalexin。一起,我们 CYP82C2 可以在导出 Trp 的第二等的代谢物的新陈代谢扮演在下面的一个假设在 JA 在哪个铺平调节的结果支持被提高。jah1-1 异种应该因此在向理解位于调停 JA 的微分回答的复杂性下面的机制的未来研究是重要的,它是重要的让植物使他们的生长适应千变万化环境。 | Fang Liu Hongling Jiang Songqing Ye Wen-Ping Chen Wenxing Liang Yingxiu Xu Bo Sun Jiaqiang Sun Qiaomei Wang Jerry D Cohen Chuanyou Li | 2010 | Cell Research2010，20，5： | 10 |
| 10 | The Arabidopsis homologs of CCR4-associated factor 1 show mRNA deadenylation activity and play a role in plant defence responses显示文摘在真核细胞的房间的送信人 RNA (mRNA ) 周转以弄短开始 poly 在 3 鈥 ? 结束的(A) 尾巴,一个过程叫了 deadenylation。在酵母, deadenylation 反应被 CCR4 和联系 CCR4 的因素主要调停 1 (CAF1 ) ,描述得好的蛋白质建筑群的二个部件 CCR4 不说出。我们这里报导那 AtCAF1a 和 AtCAF1b,酵母 CAF1 基因的通常认为的 Arabidopsis 相当或相同的事物,部分面对咖啡因或在高温度补充酵母 caf1 异种的生长缺点。AtCAF1a 和 AtCAF1b 的表示被多重压力相关的荷尔蒙和刺激导致。在 vitro 的 AtCAF1a 和在预言的活跃地点的点变化破坏这项活动。破坏 AtCAF1a 或 AtCAF1b 的表示的 T-DNA 插入异种在压力相关的 mRNAs 的 deadenylation 是有缺点的,显示二 AtCAF1 蛋白质涉及在 vivo 的调整 mRNA deadenylation。有趣地, AtCAF1a 和 AtCAF1b 表演的单个、双的异种减少了表示致病相关( PR )基因 PR1 和 PR2 并且更产生 Pseudomonas syringae pv 西红柿 DC3000 ( Pst DC3000 )感染,而转基因的植物过去表示的 AtCAF1a 表演提高了 PR1 和 PR2 的表示并且增加了抵抗到一样的病原体。我们的结果在到病原体感染的调整 mRNA deadenylation 和防卫回答建议 AtCAF1 蛋白质的角色。 | Wenxing Liang Changbao Li Fang Liu Hongling Jiang Shuyu Li Jiaqiang Sun Xiaoyan Wu Chuanyou Li | 2009 | Cell Research2009，19，3： | 8 |
| 11 | Rapid breeding of pink-fruited tomato hybrids using the CRISPR/Cas9 system显示文摘As one of the most important vegetables,tomato (Solanum lycopersicum) is extensively produced and consumed worldwide and substantially contributes to human nutrition and health (The Tomato Genome Consortium,2012).Although red tomatoes are the most common,pink tomatoes are more popular in Asia,particularly in China and Japan,because of their better taste (Ballester et al.,2010;Zhu et al.,2018).Compared with red tomatoes,pink tomatoes fail to accumulate the yellow-colored flavonoid pigment,naringenin chalcone (NarCh),in their peels,resulting in a colorless peel phenotype (Adato et al,2009;Ballester et al.,2010). | Tianxia Yang Lei Deng Wei Zhao Ruoxi Zhang Hongling Jiang Zhibiao Ye Chang-Bao Li Chuanyou Li | 2019 | Journal of Genetics and Genomics2019，46，10： | 8 |
| 12 | Rapid and efficient method for isolating plant high molecular weight(HMW)DNA with high purity显示文摘PREPARATION of HMW DNA (Megabase-size) is the basis for construction of genomic library with large DNA inserts such as bacterial artificial chromosome (BAC) and yeast artificial chromosome (YAC), and for long-range physical mapping. It can also be used for the macro-study of repeat sequences. Since HMW DNA during preparation is inclined to be sheared physically and digested by internal nucleases, it is very difficult to prepare the HMW DNA. Initially, plant HMW DNA was prepared by embedding protoplasts in the low melting-point (LMP) agarose; however, it had several disadvantages: (ⅰ) Culture of protoplasts was time-consuming, costly and tedious. ( ⅱ ) It was only used successfully for limited | Fang Qiu Jianmin Fu Weiwu Xie Chuanyou Li Bin Wang | 1999 | Chinese Science Bulletin1999，44，4： | 4 |
| 13 | Oryza sativa mediator subunit OsMED25 interacts with OsBZR1 to regulate brassinosteroid signaling and plant architecture in rice显示文摘Brassinosteroids(BRs)are plant-specific steroid hormones which regulate plant growth,development,and adaptation.Transcriptional regulation plays key roles in plant hormone signaling.A mediator can serve as a bridge between gene-specific transcription factors and the RNA polymerase machinery,functioning as an essential component in regulating the transcriptional process.However,whether a mediator is involved in BR signaling is unknown.Here,we discovered that Oryza sativa mediator subunit 25(Os MED25)is an important regulator of rice BR signaling.Phenotypic analyses showed that the Os MED25-RNAi and osmed25 mutant presented erect leaves,as observed in BR-deficient mutants.In addition,the Os MED25-RNAi and osmed25 mutant exhibited decreased BR sensitivity.Genetic analysis indicated that Os MED25-RNAi could suppress the enhanced BR signaling phenotype of Osbzr1-D.Further biochemical analysis showed that Os MED25 interacts with Os BZR1 in vivo,and Os MED25 is enriched on the promoter of Os BZR1 target genes.RNA sequencing analysis indicated that Os MED25 affects the expression of approximately 45%of Os BZR1-regulated genes and mainly functions as a corepressor of Os BZR1.Together,these findings revealed that Os MED25 regulates rice BR signaling by interacting with Os BZR1 and modulating the expression of Os BZR1 target genes,thus expanding our understanding of the roles of mediators in plant hormone signaling. | Yuekun Ren Xiaojie Tian Shuyu Li Enyang Mei Mingliang He Jiaqi Tang Min Xu Xiufeng Li Zhenyu Wang Chuanyou Li Qingyun Bu | 2020 | Journal of Integrative Plant Biology2020，62，6： | 3 |
| 14 | Association between interferon gamma receptor 1-56C/T gene polymorphism and tuberculosis susceptibility: a meta-analysis显示文摘 | Wang Wei Ren Weicong Zhang Xuxia Liu Yi Li Chuanyou | 2014 | Chinese Medical Journal2014，，21： | 3 |
| 15 | An automated approach for global identification of sRNA-encoding regions in RNA-Seq data from Mycobacterium tuberculosis显示文摘用 RNA-Seq 技术的细菌的 transcriptomes 深定序使识别小非编码的 RNA 可能,响应改变环境调整基因表示的 RNA 分子,在在初核质的 ever-increasingrange 的染色体宽的规模上。然而,为在这些大 datasetsis 识别 sRNA 候选人缺乏的一个简单、可靠的自动化方法。在从 Mycobacterium 肺结核 H37Rv 的一种指数的阶段文化产生 transcriptome 以后,这里,我们为 sRNA 的染色体宽的鉴定开发了并且验证一个自动化方法包含候选人的 regionswithin RNA-Seq 数据集基于特征的分析读范围地图。我们在 intergenic 区域识别了 192 个新奇编码 candidatesRNA 区域 ? 并且 664 个 RNA 抄本从区域 antisense 抄录了(作为) 打开 readingframes (ORF ) ,它忍受 asRNAs 的特征,并且验证了这些中的 28 个由 northernblotting 的新奇编码 sRNA 区域。我们的工作不仅在 RNA-Seq 数据为候选人 sRNA-encodingregions 的染色体宽的鉴定提供了一个简单自动化方法,而且也揭开了在 M 的许多新奇候选人编码 sRNA 区域。肺结核,增强在细菌的基因表示的控制是更多的建筑群比的看法以前期望了。 | Ming Wang Joy Fleming Zihui Li Chuanyou Li Hongtai Zhang Yunxin Xue Maoshan Chen Zongde Zhang Xian-En zhang Lijun Bi | 2016 | Acta Biochimica et Biophysica Sinica2016，48，6： | 2 |
| 16 | Rice seed identification by computerized AFLP-DNA fingerprinting显示文摘Wedevelopedacomputerizedseedidentificationsystem.FifteenricevarietiesthatwerewidelyusedinChinawereanalyzedbyAFLPfingerprinting.12primerpairswerescreened.Inordertosimplifytheprocedureandcutdownthecostinseedidentification,theleastnumberofprimerpairsforpracticalseedidentifi cationshouldbeselected. | CHEN Yihua JIA Jianhang LI Chuanyou WANG Bin WENG Manli Inst of Genetics,Chinese Academy of Sci,Beijing 100101,China | 2000 | Chinese Rice Research Newsletter2000，8，1： | 2 |
| 17 | Toward understanding the molecular mechanisms governing plant hormone actions:A briefintroduction to the Major Research Program “Molecular mechanisms of plant hormone actions” funded by the National Natural Science Foundation of China (NSFC)显示文摘Decades of physiological and molecular studies have demonstrated that plants rely on a diverse set of small molecule hormones to regulate every aspect of their biological processes including development,growth and adaptation. | LI ChuanYou LI JiaYang | 2010 | Chinese Science Bulletin2010，55，21： | 2 |
| 18 | WRKY33-mediated indolic glucosinolate metabolic pathway confers resistance against Alternaria brassicicola in Arabidopsis and Brassica crops显示文摘The tryptophan(Trp)-derived plant secondary metabolites,including camalexin,4-hydroxyindole-3-carbonylnitrile,and indolic glucosinolate(IGS),show broad-spectrum antifungal activity.However,the distinct regulations of these metabolic pathways among different plant species in response to fungus infection are rarely studied.In this study,our results revealed that WRKY33 directly regulates IGS biosynthesis,notably the production of 4-methoxyindole-3-ylmethyl glucosinolate(4MI3G),conferring resistance to Alternaria brassicicola,an important pathogen which causes black spot in Brassica crops.WRKY33 directly activates the expression of CYP81F2,IGMT1,and IGMT2 to drive sidechain modification of indole-3-ylmethyl glucosinolate(I3G)to 4MI3G,in both Arabidopsis and Chinese kale(Brassica oleracea var.alboglabra Bailey).However,Chinese kale showed a more severe symptom than Arabidopsis when infected by Alternaria brassicicola.Comparative analyses of the origin and evolution of Trp metabolism indicate that the loss of camalexin biosynthesis in Brassica crops during evolution might attenuate the resistance of crops to Alternaria brassicicola.As a result,the IGS metabolic pathway mediated by WRKY33 becomes essential for Chinese kale to deter Alternaria brassicicola.Our results highlight the differential regulation of Trp-derived camalexin and IGS biosynthetic pathways in plant immunity between Arabidopsis and Brassica crops. | Han Tao Huiying Miao Lili Chen Mengyu Wang Chuchu Xia Wei Zeng Bo Sun Fen Zhang Shuqun Zhang Chuanyou Li Qiaomei Wang | 2022 | Journal of Integrative Plant Biology2022，64，5： | 2 |
| 19 | Regulation of jasmonate signaling by reversible acetylation of TOPLESS in Arabidopsis显示文摘The plant hormone jasmonate(JA)regulates plant immunity and adaptive growth by orchestrating a genome-wide transcriptional program.Key regulators of JA-responsive gene expression include the master transcription factor MYc2,which is repressed by the conserved Groucho/Tup1-like corepressor TOPLESS(TPL)in the resting state.However,the mechanisms underlying TPL-mediated transcriptional repression of MYc2 activity and hormone-dependent switching between repression and de-repression remainenigmatic.Here,we report the regulation of TPLactivity and JAsignaling byreversibleacetylation of TPL.We found that the histone acetyltransferase GCN5 could mediate TPL acetylation,which enhances its interaction with the NOVEL-INTERACTOR-OF-JAZ(NINJA)adaptor and promotes its recruitment to MYc2 target promoters,facilitating transcriptional repression.Conversely,TPL deacetylation by the histonedeacetylase HDA6 weakens TPL-NINJA interaction and inhibitsTPL recruitmentto MYC2 target promoters,facilitating transcriptional activation.In the resting state,the opposing activities of GCN5 and HDA6 maintain TPL acetylation homeostasis,promoting transcriptional repression activity of TPL.In response to JA elicitation,HDA6 expression is transiently induced,resulted in decreased TPL acetylation and repressor activity,thereby transcriptional activation of MYC2 target genes.Thus,the GCN5-TPL-HDA6 module main tains the homeostasis of acetylated TPL,thereby determining the transcriptional state of JA-responsive genes.Our findings uncovered a mechanism by which the TPL corepressor activity in JA signaling is activelytuned inarapidandreversiblemanner. | Chunpeng An Lei Deng Huawei Zhai Yanrong You Fangming Wu Qingzhe Zhai Alain Goossens Chuanyou Li | 2022 | Molecular Plant2022，15，8： | 2 |
| 20 | Construction and characterization of a bacterial artificial chromosome library of rice 5460F显示文摘Thermo-sensitive genie male sterile (TGMS) rice has a number of desirable characteristics for hybrid rice production. Many studies have demonstrated that the sterility of TGMS rice is controlled by a single recessive gene. It has been mapped for the first time on chromosome 8 and named tms1. Several AFLP markers which tightly linked to the tms1 gene have been identified recently. In order to develop a detailed physical map of the tms1 gene-encompassing region and finally clone the tms1 gene, a bacterial artificial chromosome (BAC) | Jianmin Fu Xueping Qu Chuanyou Li Jianhang Jia Demin Jin Qian Wang Rencui Yang Bin Wang | 1999 | Chinese Science Bulletin1999，44，17： | 2 |
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