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| 1 | Effects of lysedEnterococcus faecalis FK-23 on experimental allergic rhinitis in a murine model显示文摘In the current study,we sought to investigate whether lysed Enterococcus faecalis FK-23(LFK),a heat-killed probiotic preparation,attenuated eosinophil influx into the upper airway and had immunomodulatory activity in a murine allergic rhinitis model.Eighteen BALB/c mice were divided into three groups;the ovalbumin(OVA)-sensitized/challenged group,which received saline orally for 6 weeks(OVA group),the OVA-sensitized/challenged group,which received LFK orally for 6 weeks(LFK-fed group),and the non-sensitized group,which received saline for 6 weeks(saline control group).Nasal rubbing and sneezing were monitored during the study.After the final challenge,interleukin(IL)-4,interferon(IFN)-γ,and OVA-specific IgE levels in the sera and splenocyte culture supernatants were determined,eosinophilic infiltrate into the upper airway was quantified,and splenic CD4+CD25+ regulatory T cells(Tregs) were examined by flow cytometry.We found that nasal rubbing was significantly reduced in LFK-fed mice compared to the OVA group on d 27 and 35,and sneezing was significantly inhibited by LFK administration for 35 d.LFK-fed mice had significantly less eosinophil influx into the nasal mucosa than the OVA group.There were no significant differences between the LFK-fed group and OVA group in the serum and splenocyte culture supernatant levels of IL-4,IFN-γ,and OVA-specific IgE.Interestingly,the LFK-fed mice had a significantly greater percentage of splenic CD4+CD25+ Tregs than OVA group.Our results indicate that oral administration of LFK may alleviate nasal symptoms,reduce nasal eosinophilia,and increase the percentage of CD4+CD25+ Tregs in experimental allergic rhinitis. | Luping Zhu Takashi Shimada Ruoxi Chen Meiping Lu Qingzhao Zhang Wenmin Lu Min Yin Tadao Enomoto Lei Cheng | 2012 | The Journal of Biomedical Research2012，26，3： | 11 |
| 2 | Expression profiling-based clustering of healthy subjects recapitulates classifications defined by clinical observation in Chinese medicine显示文摘Differences between healthy subjects and associated disease risks are of substantial interest in clinical medicine. Based on clinical presentations, Traditional Chinese Medicine(TCM) classifies healthy people into nine constitutions: Balanced, Qi, Yang or Yin deficiency, Phlegm-dampness, Damp-heat, Blood stasis,Qi stagnation, and Inherited special constitutions. In particular, Yang and Yin deficiency constitutions exhibit cold and heat aversion, respectively. However, the intrinsic molecular characteristics of unbalanced phenotypes remain unclear. To determine whether gene expression-based clustering can recapitulate TCM-based classification, peripheral blood mononuclear cells(PBMCs) were collected from Chinese Han individuals with Yang/Yin deficiency(n ? 12 each) and Balanced(n ? 8) constitutions, and global gene expression profiles were determined using the Affymetrix HG-U133 A Plus 2.0 array. Notably,we found that gene expression-based classifications reflected distinct TCM-based subtypes. Consistent with the clinical observation that subjects with Yang deficiency tend toward obesity, series-clustering analysis detected several key lipid metabolic genes(diacylglycerol acyltransferase(DGAT2), acyl-Co A synthetase(ACSL1), and ATP-binding cassette subfamily A member 1(ABCA1)) to be down-and upregulated in Yin and Yang deficiency constitutions, respectively. Our findings suggest that Yin/Yang deficiency and Balanced constitutions are unique entities in their m RNA expression profiles. Moreover,the distinct physical and clinical characteristics of each unbalanced constitution can be explained, in part,by specific gene expression signatures. | Ruoxi Yu Dan Liu Yin Yang Yuanyuan Han Lingru Li Luyu Zheng Ji wang Yan Zhang Yingshuai Li Qian-Fei Wang Qi wang | 2017 | Journal of Genetics and Genomics2017，44，4： | 9 |
| 3 | Transcriptional landscape of rice roots at the single-cell resolution显示文摘There are two main types of root systems in flowering plants,namely taproot systems of dicots and fibrous root systems found in monocots.Despite this fundamental split,our current knowledge of cellular and molecular mechanism driving root development is mainly based on studies of the dicot model Arabidopsis.However,the world major crops are monocots and little is known about the transcriptional programs underlying cell-type specification in this clade.Here,we report the transcriptomes of more than 20000 single cells derived from root tips of two agronomically important rice cultivars.Using combined computational and experimental analyses we were able to robustly identify most of the major cell types and define novel cell-type-specific marker genes for both cultivars.Importantly,we found divergent cell types associated with specific regulatory programs,including phytohormone biosynthesis,signaling,and response,which were well conserved between the two rice cultivars.In addition,we detected substantial differences between the cell-type transcript profiles of Arabidopsis and rice.These species-specific features emphasize the importance of analyzing tissues across diverse model species,including rice.Taken together,our study provides insight into the transcriptomic landscape of major cell types of rice root tip at single-cell resolution and opens new avenues to study cell-type specification,function,and evolution in plants. | Qing Liu Zhe Liang Dan Feng Sanjie Jiang Yifan Wang Zhuoying Du Ruoxi Li Guihua Hu Pingxian Zhang Yanfei Ma Jan ULohmann Xiaofeng Gu | 2021 | Molecular Plant2021，14，3： | 9 |
| 4 | Allele-defined genome reveals biallelic differentiation during cassava evolution显示文摘Dear Editor,Generation of heterozygous genomes by hybridization between or within species can help maintain plant diversity and serve as a potential source of new species(Baek et al.,2018).Moreover,genomic heterozygosity is associated with genomic coadaptation,developmental stability,and heterosis.Accurate definition of alleles in haplotypes is necessary to precisely characterize allelic variation controlling agriculturally important traits(Shi et al.,2019).Currently,most released genomes have mosaic assembly of haplotypes due to random selection or collapse of alleles during genome assembly(Shi et al.,2019),which masked allelic variation and functional differentiation of divergent alleles in heterozygous species. | Wei Hu Changmian Ji Haitao shi Zhe Liang Zehong Ding Jianqiu Ye Wenjun Ou Gang Zhou Weiwei Tie Yan Yan Jinghao Yang Xiaoying Yang Yunxie Wei Zhiqiang Jin Jianghui Xie Ming Peng Wenquan Wang Anping Guo Biyu Xu Jianchun Guo Songbi Chen Liming Ma Mingcheng Wang Yang Zhou Xiaolong Li Ruoxi Li Siyi Guo Xinhui Xiao Zhongqing Wan Feifei An Jie Zhang Qingyun Leng Yin Li Ray Ming Kaimian Li | 2021 | Molecular Plant2021，14，6： | 8 |
| 5 | Rapid breeding of pink-fruited tomato hybrids using the CRISPR/Cas9 system显示文摘As one of the most important vegetables,tomato (Solanum lycopersicum) is extensively produced and consumed worldwide and substantially contributes to human nutrition and health (The Tomato Genome Consortium,2012).Although red tomatoes are the most common,pink tomatoes are more popular in Asia,particularly in China and Japan,because of their better taste (Ballester et al.,2010;Zhu et al.,2018).Compared with red tomatoes,pink tomatoes fail to accumulate the yellow-colored flavonoid pigment,naringenin chalcone (NarCh),in their peels,resulting in a colorless peel phenotype (Adato et al,2009;Ballester et al.,2010). | Tianxia Yang Lei Deng Wei Zhao Ruoxi Zhang Hongling Jiang Zhibiao Ye Chang-Bao Li Chuanyou Li | 2019 | Journal of Genetics and Genomics2019，46，10： | 8 |
| 6 | A Positive Correlation between Elevated Altitude and Frequency of Mutant Alleles at the EPAS1 and HBB Loci in Chinese Indigenous Dogs显示文摘Hypoxia represents one of the most extreme environmental conditions for both human beings and animals living at high altitudes(Zhao et al.,2009).Over the past few years,great attention has been focused on the genetic bases of adaption to high-altitude environments(Bigham et al.,2010;Simonson et al.,2010).The domestic dog(Canis familiaris)is the first animal that | Ruoxi Fan Fei Liu Hong Wu Shifang Wu Chunling Zhu Yan Li Guodong Wang Yaping Zhang | 2015 | Journal of Genetics and Genomics2015，42，4： | 4 |
| 7 | Effect of Beam Current and Diameter on Electron Probe Microanalysis of Carbonate Minerals显示文摘The effect of operating conditions on the time-dependent X-ray intensity variation is of great importance for the optimal EPMA conditions for accurate determinations of various elements in carbonate minerals. Beam diameters of 0, 1, 2, 5, 10, 15, and 20 μm, and beam currents of 3, 5, 10, 20,and 50 nA were tested. Ca, Mg, Zn, and Sr were found to be more sensitive to electron beam irradiation as compared to other elements, and small currents and large beam diameters minimized the timedependent X-ray intensity variations. We determined the optimal EPMA operating conditions for elements in carbonate: 10 μm and 5 nA for calcite;10 μm and 10 nA for dolomite;5 μm and 10 nA or 10 μm and 20 nA for strontianite;and 20 nA and 5 μm for other carbonate. Elements sensitive to electron beam irradiation should be determined first. In addition, silicate minerals are preferred as standards rather than carbonate minerals. | Xing Zhang Shuiyuan Yang He Zhao Shaoyong Jiang Ruoxi Zhang Jing Xie | 2019 | Journal of Earth Science2019，30，4： | 3 |
| 8 | Consistency between traditional Chinese medicine constitution-based classification and genetic classification显示文摘Background:We studied the consistency between two classification systems for categorizing patients:traditional Chinese medicine(TCM)constitution-based methods,versus genetic clustering.Genetic classification in constitutional identification was also evaluated.Methods:A TCM physician evaluated the constitution of each patient,according to four examinations(inspection,auscultation-olfaction,interrogation,and palpation).Those who met the criteria for Yang-deficient,Yin-deficient,and balanced constitutions were enrolled in the study.Peripheral blood samples were obtained from the participants,and peripheral blood mononuclear cells were separated from the samples within 2 hours.Total RNA extraction from the white blood cells was performed;and an Affymetrix HG-U133 Plus2.0 array was used to determine the peripheral blood gene expression profiles.The samples were classified using a support vector machine genetic classifier,and the“leave-one-out”method was used for validation.Results:The global gene expression profiles of 32 samples were grouped into three categories,and the samples in each of the gene categories corresponded with the three constitution categories.The three constitution types were distinguished using the genetic classifier with 165 genes.The accuracy of the prediction classification was greater than 95%using mathematical method.Conclusions:Participants with Yin-deficient,Yang-deficient,and balanced constitutions have varying physical characteristics and gene expression patterns.Additionally,the results from TCM constitution classification matched those obtained by genetic classification.Finally,our preliminary gene classifier distinguishes among Yin-deficient,Yang-deficient,and balanced constitutions,and provides a methodological basis for identifying the different constitutions. | Ruoxi Yu Xiaohong Zhao Lingru Li Cheng Ni Yin Yang Yuanyuan Han Ji Wang Yan Zhang Qi Wang | 2015 | Journal of Traditional Chinese Medical Sciences2015，2，4： | 2 |
| 9 | Estimation of the age of human semen stains by attenuated total reflection Fourier transform infrared spectroscopy:a preliminary study显示文摘Semen stain is one of the most important biological evidence at sexual crime scenes.Age estimation of human semen stains plays an important role in forensic work,and it is rarely studied due to lack of well-established methods.In this study,the technique called attenuated total reflection Fourier transform infrared spectroscopy(ATR-FTIR)coupled with advanced chemometric methods was employed to determine the age of semen stains on three different substrates:glass slides,tissues and fabric made of regenerated cellulose fibres up to 6 d.Partial least squares regression(PLSR)was used in conjunction with spectral analysis for age estimation,and the results generated high R2 values(cross-validation:0.81,external validation:0.74)but a narrow margin of error for root mean square error(RMSE)(RMSE of cross-validation:0.77 d,RMSE of prediction:1.02 d).Additionally,our results indicated the robustness of PLSR model was not weaken by the influence of different substrates in this study.Our results indicate that ATR-FTIR,combined with chemometric methods,shows great potential as a convenient and efficient tool for age estimation of semen stains.Moreover,the method could be applied to routine forensic investigations in the future. | Shuai Zha Xin Wei Ruoxi Fang Qi Wang Hancheng Lin Kai Zhang Haohui Zhang Ruina Liu Zhouru Li Ping Huang Zhenyuan Wang | 2020 | Forensic Sciences Research2020，5，2： | 2 |
| 10 | The STING 1 network regulates autophagy and cell death显示文摘Cell death and immune response are at the core of life.In past decades,the endoplasmic reticulum(ER)protein STING!(also known as STING or TMEM173)was found to play a fundamental role in the production of type I interferons(IFNs)and pro-inflammatory cytokines in response to DNA derived from invading microbial pathogens or damaged hosts by activating multiple transcription factors.In addition to this well-known function in infection,inflammation,and immunity,emerging evidence suggests that the STING 1-dependent signaling network is implicated in health and disease by regulating autophagic degradation or various cell death modalities(e.g.,apoptosis,necroptosis,pyroptosis,ferroptosis,mitotic cell death,and immunogenic cell death[ICD]).Here,we outline the latest advances in our understanding of the regulating mechanisms and signaling pathways of STING1 in autophagy and cell death,which may shed light on new targets for therapeutic interventions. | Ruoxi Zhang Rui Kang Daolin Tang | 2021 | Signal Transduction and Targeted Therapy2021，6，7： | 2 |
| 11 | Defect of SLC38A3 promotes epithelial-mesenchymal transition and predicts poor prognosis in esophageal squamous cell carcinoma显示文摘Objective: Solute carrier family 38(SLC38 s) transporters play important roles in amino acid transportation and signaling transduction. However, their genetic alterations and biological roles in tumors are still largely unclear.This study aimed to elucidate the genetic signatures of SLC38 s transporters and their implications in esophageal squamous cell carcinoma(ESCC).Methods: Analyses on somatic mutation and copy number alterations(CNAs) of SLC38 A3 were performed as described. Immunohistochemistry(IHC) assay and Western blot assay were used to detect the protein expression level. MTS assay, colony formation assay, transwell assay and wound healing assay were used to explore the malignant phenotypes of ESCC cells. Immunofluorescence assay was used to verify the colocalization of two indicated proteins and immunopreciptation assay was performed to confirm the interaction of proteins.Results: Our findings revealed that SLC38 s family was significantly disrupted in ESCC, with high frequent CNAs and few somatic mutations. SLC38 A3 was the most frequent loss gene among them and was linked to poor survival and lymph node metastasis. The expression of SLC38 A3 was lower in tumor tissues compared to that in normal tissues, which was also significantly associated with worse clinical outcome. Further experiments revealed that depletion of SLC38 A3 could promote EMT in ESCC cell lines, and the interaction of SLC38 A3 and SETDB1 might lead to the reduced transcription of Snail. Pharmacogenomic analyses demonstrated that fifteen inhibitors were showed significantly correlated with SLC38 A3 expression.Conclusions: Our investigations have provided insights that SLC38 A3 could act as a suppressor in EMT pathway and serve as a prognostic factor and predictor of differential drug sensitivities in ESCC. | Rui Liu Ruoxi Hong Yan Wang Ying Gong Danna Yeerken Di Yang Jinting Li Jiawen Fan Jie Chen Weimin Zhang Qimin Zhan | 2020 | Chinese Journal of Cancer Research2020，32，5： | 1 |
| 12 | Microstructures and thermochromic characteristics of low-cost vanadium–tungsten co-sputtered thin films显示文摘 | Sheng Zhou Yi Li Huiqun Zhu Ruoxi Sun Yuming Zhang Yize Huang Liu Li Yujian Shen Qiuxin Zhen Guoxiang Tong Baoying Fang | 2011 | Surface & Coatings Technology (-)2011，，11： | 1 |
| 13 | Urotensin Ⅱ receptor antagonist reduces hepatic resistance and portal pressure through enhanced eNOS-dependent HSC vasodilatation in CCI4-induced cirrhotic rats显示文摘Increased serum urotensin Ⅱ(UⅡ) levels in human cirrhotic populations have been recently shown,but the long-term effects of UⅡ receptor antagonist on the cirrhosis have not been investigated.To investigate the therapeutic effects of urotensin Ⅱ receptor (UT) antagonist palosuran on rats with carbon tetrachloride (CCI4)- induced cirrhosis,the hepatic and systemic hemodynamics,liver fibrosis,the metalloproteinase-13 (MMP-13)/ tissue inhibitor of metalloproteinase-1 (TIMP-1) ratio,hepatic Rho-kinase activity,and the endothelial nitric oxide synthase (eNOS) activity are measured in CCI4-cirrhotic rats treated with palosuran or vehicle for 4 weeks.Primary hepatic stellate cells (HSCs) are used to investigate the changes in UⅡ/UT expression and the in vitro effect of palosuran.Compared with the vehicle-treated cirrhotic rats,treatment with palosuran can reduce the portal pressure (PP),decrease the risk of liver fibrosis and the level of a smooth muscle actin,collagen-l (COL-I),and transforming growth factor β expression.However,treatment with palosuran can increase MMP-13/TIMP-1,pvasodilator- stimulated phosphoprotein (p-VASP),and p-eNOS expression.Moreover,in vitro UⅡ/UT mRNA expression increases during HSC activation.MMP-13/TIMP-1,COL-I,and p-VASP are inhibited after palosuran treatment Our data indicate that long-term administration of palosuran can decrease PP in cirrhosis,which results from decreased hepatic fibrosis and enhanced eNOS-dependent HSC vasodilatation. | Ruoxi Zhang Jing Chen Diangang Liu Yu Wang | 2019 | Frontiers of Medicine2019，13，3： | 1 |
| 14 | 美国一般人群尿镉水平与心电图改变的关系显示文摘[目的]观察美国一般人群的尿镉水平与心电图改变的关系,了解镉暴露对心电图的影响。[方法]利用美国第三次国家健康与营养调查(NHANESⅢ)中进行心电图测量,年龄≥40岁的6 889名参与者作为研究对象。采用标准十二导联心电图分析系统记录心电图参数;采用原子吸收光谱法测定尿镉含量。按性别分层,运用多元线性回归分析尿镉水平与心电图改变的关系。[结果]尿镉平均水平为(0.7±0.01)μg/g肌酐,其中男性人群平均(0.6±0.02)μg/g肌酐;女性(0.8±0.02)μg/g肌酐;在男性群体,尿镉水平四分位最高与最低等分相比,多因素调整的心率、PRrr间期、QTrr间期和QRS间期变化及其95%CI分别为1.72(0.05~3.4)次/分,-3.87(-7.3^-0.45)ms,-0.39(-4.09~3.22)ms和-2.64(-4.96^-0.33)ms。在女性群体,尿镉水平最高与最低等分相比,上述相应的心电图参数变化及其95%CI分别为-0.19(-1.72~1.33)次/分,-0.09(-4.13~3.94)ms,1.59(-1.05~4.23)ms和-1.11(-2.68~0.47)ms。[结论]尿镉水平与美国男性一般人群的心电图改变存在一定的关联。 | 黎小飞 Eliseo GUALLAR Ruoxi LIN Yiyi ZHANG 钟秋安 | 2015 | 环境与职业医学2015，32，3： | 1 |
| 15 | Empirical Research on Housing Condition of Rural Migrant Workers-A Case Study of Nanning in China 显示文摘 | Xiekui Zhang Jing Zhang Ruoxi Liu | 2011 | Applied Economics Business and Development--Communications in Computer and Information tScience2011，208，： | 1 |
| 16 | A novel aluminumdoped carbon nanotubes sensor for carbon monoxide显示文摘 | Ruoxi Wang Dongju Zhang Wenqi Sun | 2007 | Journal of Molecular Structure Theochem2007，，806： | 1 |
| 17 | Method for the extraction of circulating nucleic acids based on MOF reveals cell-free RNA signatures in liver cancer显示文摘Cell-free RNA(cf RNA) allows assessment of health, status, and phenotype of a variety of human organs and is a potential biomarker to non-invasively diagnose numerous diseases. Nevertheless, there is a lack of highly efficient and bias-free cf RNA isolation technologies due to the low abundance and instability of cf RNA.Here, we developed a reproducible and high-efficiency isolation technology for different types of cell-free nucleic acids(containing cf RNA and viral RNA) in serum/plasma based on the inclusion of nucleic acids by metal-organic framework(MOF) materials, which greatly improved the isolation efficiency and was able to preserve RNA integrity compared with the most widely used research kit method. Importantly, the quality of cf RNA extracted by the MOF method is about 10-fold that of the kit method, and the MOF method isolates more than three times as many different RNA types as the kit method. The whole transcriptome mapping characteristics of cf RNA in serum from patients with liver cancer was described and a cf RNA signature with six cf RNAs was identified to diagnose liver cancer with high diagnostic efficiency(area under curve = 0.905 in the independent validation cohort) using this MOF method. Thus, this new MOF isolation technique will advance the field of liquid biopsy, with the potential to diagnose liver cancer. | Yuqing Sun Haixin Yu Shaoqing Han Ruoxi Ran Ying Yang Yongling Tang Yuhao Wang Wenhao Zhang Heng Tang Boqiao Fu Boshi Fu Xiaocheng Weng Song-Mei Liu Hexiang Deng Shuang Peng Xiang Zhou | 2024 | National Science Review2024，11，1： | 0 |
| 18 | Experimental Method of Cosmetics Human Efficacy Evaluation-Whitening显示文摘This paper briefly introduces the human body evaluation test method of cosmetics whitening efficacy test,the measurement method of skin melanin content,skin chromaticity and the instruments used,so as to provide ideas for scientific and rigorous test scheme design and scientific basis for whitening efficacy claim. | Zhang Hongyan Wang Peng Guo Ruoxi Zhang Han Zhang Lei | 2022 | China Detergent & Cosmetics2022，7，1： | 0 |
| 19 | Experimental Method of Cosmetics Human Efficacy Evaluation——Moisturizing显示文摘This paper briefly introduces the human body evaluation test method of cosmetics moisturizing efficacy test,according to stratum corneum moisture content test,skin moisturizing capacity test and other test methods and the instruments used are briefly introduced,so as to provide ideas for scientific and rigorous experimental scheme design and scientific basis for the claim of moisturizing effect. | Guo Ruoxi Wang Peng Zhang Hongyan Zhang Lei | 2021 | China Detergent & Cosmetics2021，6，3： | 0 |
| 20 | Experimental Method Of Cosmetics Human Efficacy Evaluation-Delaying Skin Aging Efficacy显示文摘This paper briefly introduce the test method for human evaluation of cosmetics to delay skin aging,and provide reference for the test of relevant indexes.By formulating a reasonable test plan and choosing appropriate equipment to test the improvement of the skin aging characteristics before and after the product is used,it can provide a scientific basis for claiming the effect of delaying skin aging. | Wang Peng Zhang Hongyan Sha Ou Guo Ruoxi Zhang Han Zhang Lei | 2021 | China Detergent & Cosmetics2021，6，4： | 0 |
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