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| 1 | Knockdown of OsHox33, a member of the class III homeodomain-leucine zipper gene family, accelerates leaf senescence in rice显示文摘The class III homeodomain-leucine zipper(HD-Zip III)gene family plays important roles in plant growth and development,including regulation of apical embryo patterning,embryonic shoot meristem formation,leaf polarity,vascular development,and meristem function,with a particularly crucial function in leaf development.Although HD-Zip III members are highly conserved in land plants,previous studies,such as genetic analyses based on multiple mutants in Arabidopsis and other plants,suggest that various HD-Zip III family genes have evolved with distinct functions and pleiotropic effects on plant growth and development.In this study,we analyzed a HD-Zip III member,OsHox33,and demonstrated that it plays an important role in age-dependent leaf senescence in rice.We constructed two specific RNAi vectors derived from the 5′-end region and 3′-UTR of OsHox33 to knockdown its expression.Transgenic plants harboring either RNAi construct displayed similar phenotypes of precocious leaf senescence symptoms,suggesting that knockdown of OsHox33 accelerates leaf senescence in rice.pOsHox33::GUS fusion expression and RT-PCR revealed that OsHox33 is highly expressed in young organs,especially in young meristems such as shoot apical meristems,intercalary meristems,and young callus.In addition,real-time PCR indicated that OsHox33 was more highly expressed in young leaves than in old leaves.To further investigate OsHox33 function,we analyzed chloroplast ultrastructure in different-aged leaves of RNAi plants,and found that OsHox33 knockdown accelerated chloroplast degradation,which is consistent with RNAi phenotypes.Finally,real-time PCR studies showed that OsHox33 can regulate the expression of GS1 and GS2,two senescence-associated genes.Taken together,the work presented here provides new insights into the function of HD-Zip III members in plants. | LUAN WeiJiang SHEN Ao JIN ZhiPing SONG SuSheng LI ZhengLong SHA AiHua | 2013 | Science China(Life Sciences)2013，56，12： | 13 |
| 2 | Analysis of DNA methylation variation in wheat genetic background after alien chromatin introduction based on methylation-sensitive amplification polymorphism显示文摘During the process of alien germplasm introduced into wheat genome by chromosome engineering, extensive genetic variations of genome structure and gene expression in recipient could be induced. In this study, we performed GISH (genome in situ hybridization) and AFLP (amplified fragment length po-lymorphism) on wheat-rye chromosome translocation lines and their parents to detect the identity in genomic structure of different translocation lines. The results showed that the genome primary struc-ture variations were not obviously detected in different translocation lines except the same 1RS chromosome translocation. Methylation sensitive amplification polymorphism (MSAP) analyses on genomic DNA showed that the ratios of fully-methylated sites were significantly increased in translo-cation lines (CN12, 20.15%; CN17, 20.91%; CN18, 22.42%), but the ratios of hemimethylated sites were significantly lowered (CN12, 21.41%; CN17, 23.43%; CN18, 22.42%), whereas 16.37% were fully-me-thylated and 25.44% were hemimethylated in case of their wheat parent. Twenty-nine classes of me-thylation patterns were identified in a comparative assay of cytosine methylation patterns between wheat-rye translocation lines and their wheat parent, including 13 hypermethylation patterns (33.74%), 9 demethylation patterns (22.76%) and 7 uncertain patterns (4.07%). In further sequence analysis, the alterations of methylation pattern affected both repetitive DNA sequences, such as retrotransposons and tandem repetitive sequences, and low-copy DNA. | ZHANG Yong LIU ZhaoHui LIU Cheng YANG ZuJun DENG KeJun PENG JinHua ZHOU JianPing LI GuangRong TANG ZongXiang REN ZhengLong | 2008 | Chinese Science Bulletin2008，53，1： | 8 |
| 3 | Remotely excited Raman optical activity using chiral plasmon propagation in Ag nanowires显示文摘We experimentally investigated remotely excited Raman optical activity(ROA)using propagating surface plasmons in chiral Ag nanowires.Using chiral fmoc-glycyl-glycine-OH(FGGO)molecules,we first studied the local surface plasmon-enhanced ROA.We found that the Raman intensity can be excited by left-and right-circularly polarized lights and that the circular intensity difference(CID)can be significantly enhanced.Second,by selecting vibrational modes with large Raman and ROA intensities that are not influenced by chemical enhancements,we studied remotely excited ROA imaging and the CID of FGGO molecules by propagating a plasmonic waveguide using Ag chiral nanostructures.When laser light was radiated on one of the Ag terminals,the measured CID of the FGG at the other terminal showed little change compared to the local excited CID.Meanwhile,when the laser light was radiated on the Ag nanowires(not on the terminals)and was coupled to the nearby nanoantenna,the CID of the ROA could be manipulated by altering the coupling angle between the Ag nanowires.To directly demonstrate the propagation of ROA along the nanowire and its remote detection,we also measured the remotely excited ROA spectra.Our experimental method has the potential to remotely determine the chirality of molecular structures and the absolute configuration or conformation of a chiral live cell. | Mengtao Sun Zhenglong Zhang Peijie Wang Qiang Li Fengcai Ma Hongxing Xu | 2013 | Light(Science & Applications)2013，2，1： | 7 |
| 4 | Effects of Yishengukang decoction on expression of bone-specific alkaline phosphatase,carboxyterminal propeptide of typeⅠprocollagen,and carboxyterminal cross-linked telepeptide of typeⅠcollagen in malignant tumor patients with bone metastasis显示文摘OBJECTIVE: To investigate the effect of Yishengukang decoction on the expression of the metabolic bone markers, bone-specific alkaline phosphatase(BAP), carboxyterminal propeptide of type Ⅰ procollagen(PICP), and carboxyterminal cross-linked telepeptide of type Ⅰ collagen(ICTP), in cancer patients with bone metastasis.METHODS: Patients(n = 180) were divided into three groups:(a) bone metastasis patients treated with Yishengukang and pamidronate disodium injection(treatment group, n = 60);(b) bone metastasis patients treated with pamidronate disodium injection alone(control group, n = 60);(c) cancer patients without metastatic bone lesion(non-bone metastasis group, n = 60). Serum levels of the metabolic markers BAP, PICP, and ICTP were detected by enzyme-linked immunosorbent assay pre- and post-therapy.RESULTS: A significant decrease in serum BAP level was observed in the treatment group compared with the control group. However there were no significant differences in serum levels of PICP and ICTP before or after treatment compared with the control group.CONCLUSION: Yishengukang decoction combined with pamidronate disodium injection reduced serum BAP level to a greater extent that pamidronate disodium injection alone. Furthermore, the combined therapy was more beneficial in regulating imbalanced bone metabolism after bone metastasis,and may represent the molecular mechanism underpinning the effects of Yishengukang decoction. | Yin Yukun Feng Li Zhou Lei Li Jie Gao Yin Wang Ningjun Yu Jianhua Jiang Zhenglong He Shengqi Lu Dianrong Wang Fang Du Yanlin | 2017 | Journal of Traditional Chinese Medicine2017，37，1： | 6 |
| 5 | The alternative of CubeSat-based advanced infrared and microwave sounders for high impact weather forecasting显示文摘近年来,小卫星技术的发展给传统气象卫星提供了经济有效的替补方案。本文针对可能出现的传统气象卫星的缺失情形,借助快速的区域观测系统模拟试验,探索了利用小卫星填补空缺的可能性。研究表明,单个小卫星,无论是近红外高光谱还是微波探测仪,都能够对局地强风暴天气的预报有所改进。然而,为了填补传统卫星缺失带来的影响,需要发射三颗甚至更多的小卫星来增加观测的覆盖面,以弥补小卫星精度略低、通道略少的缺陷。 | LI Zhenglong LI Jun SCHMIT Timothy J. WANG Pei LIM Agnes LI Jinlong NAGLE Fredrick W. BAI Wenguang OTKIN Jason A. ATLAS Robert HOFFMAN Ross N. BOUKABARA Sid-Ahmed ZHU Tong BLACKWELL William J. PAGANO Thomas S. | 2019 | Atmospheric and Oceanic Science Letters2019，12，2： | 3 |
| 6 | CHARACTERISTICS OF DROPLET TRANSFER IN CO_2 LASER-MIG HYBRID WELDING WITH SHORTCIRCUITING MODE显示文摘有 4.5 公里厚度的 LF6 铝合金盘子在这个实验被焊接。焊接被使用 CO_2 激光执行 -- 米格式飞机同等在扁平的位置的轴的混合焊接。试验性的结果显示常规米格式飞机弧的固有的微滴转移周期时间在激光的电路 theshort 模式由于在 CO_2 激光横梁和米格式飞机弧之间的相互作用被改变 -- 焊接的米格式飞机混血儿。因为到电极和基础材料的 CO_2 激光的预热行动,米格式飞机弧的微滴转移频率在混合焊接进程被增加。当激光力量被增加到某个度时,微滴转移频率由于导致激光的锁眼的效果被减少。而且,通过分析 MIGwelding 电流和弧电压,波形和微滴的特征在微滴转移的频率上在混合焊接过程,激光精力的效果和在激光横梁和弧之间的行动点转,焊接外观在细节被调查。 | LEI Zhenglong CHEN Yanbin LI Liqun WU Lin | 2006 | Chinese Journal of Mechanical Engineering2006，19，2： | 3 |
| 7 | Value-added Impact of Geostationary Hyperspectral Infrared Sounders on Local Severe Storm Forecasts——via a Quick Regional OSSE显示文摘Accurate atmospheric temperature and moisture information with high temporal/spatial resolutions are two of the key parameters needed in regional numerical weather prediction(NWP) models to reliably predict high-impact weather events such as local severe storms(LSSs). High spectral resolution or hyperspectral infrared(HIR) sounders from geostationary orbit(GEO) provide an unprecedented source of near time-continuous, three-dimensional information on the dynamic and thermodynamic atmospheric fields—an important benefit for nowcasting and NWP-based forecasting. In order to demonstrate the value of GEO HIR sounder radiances on LSS forecasts, a quick regional OSSE(Observing System Simulation Experiment)framework has been developed, including high-resolution nature run generation, synthetic observation simulation and validation, and impact study on LSS forecasts. Results show that, on top of the existing LEO(low earth orbit) sounders, a GEO HIR sounder may provide value-added impact [a reduction of 3.56% in normalized root-mean-square difference(RMSD)] on LSS forecasts due to large spatial coverage and high temporal resolution, even though the data are assimilated every 6 h with a thinning of 60 km. Additionally, more frequent assimilations and smaller thinning distances allow more observations to be assimilated, and may further increase the positive impact from a GEO HIR sounder. On the other hand, with denser and more frequent observations assimilated, it becomes more difficult to handle the spatial error correlation in observations and gravity waves due to the limitations of current assimilation and forecast systems(such as a static background error covariance). The peak reduction of 4.6% in normalized RMSD is found when observations are assimilated every 3 h with a thinning distance of 30 km. | Zhenglong LI Jun LI Pei WANG Agnes LIM Jinlong LI Timothy J.SCHMIT Robert ATLAS Sid-Ahmed BOUKABARA Ross N.HOFFMAN | 2018 | Advances in Atmospheric Sciences2018，35，10： | 3 |
| 8 | Application of computational fluid dynamics simulation for submarine oil spill显示文摘Computational fluid dynamics(CFD) codes are being increasingly used in the simulation of submarine oil spills.This study focuses on the process of oil spills, from damaged submarine pipes, to the sea surface, using numerical models. The underwater oil spill model is developed, and a description of the governing equations is proposed,along with modifications required for the particalization of the control volume. Available experimental data were introduced to evaluate the validity of the CFD predictions, the results of which proved to be in good agreement with the experimental data. The effects of oil leak rate, leak diameter, current velocity, and oil density are investigated, by the validated CFD model, to estimate the undersea leakage time, the lateral migration distance,and surface diffusion range when the oil reaches the sea surface. Results indicate that the leakage time and lateral migration distance increase with decreasing leak rates and leak diameter, and increase with increasing current velocity and oil density. On the other hand, a large leak diameter, high density, high leak rate, or fast currents result in a greater surface diffusion range. The findings and analysis presented here will provide practical predictions of oil spills, and guidance for emergency rescues. | YANG Zhenglong YU Jianxing LI Zhigan CHEN Haicheng JIANG Meirong CHEN Xi | 2018 | Acta Oceanologica Sinica2018，37，11： | 3 |
| 9 | Ensemble Retrieval of Atmospheric Temperature Profiles from AIRS显示文摘Satellite-based observations provide great opportunities for improving weather forecasting. Physical retrieval of atmospheric profiles from satellite observations is sensitive to the uncertainty of the first guess and other factors. In order to improve the accuracy of the physical retrieval, an ensemble methodology was developed with an emphasis on perturbing the first guess. In the methodology, a normal probability density function(PDF) is used to select the optimal profile from the ensemble retrievals. The ensemble retrieval algorithm contains four steps:(1) regression retrieval for original first guess;(2) perturbation of the original first guess to generate new first guesses(ensemble first guesses);(3) using the ensemble first guesses and nonlinear iterative physical retrieval to generate ensemble physical results; and(4) the final optimal profile is selected from the ensemble physical results by using PDF. Temperature eigenvectors(EVs) were used to generate the perturbation and generate the ensemble first guess. Compared with the regular temperature profile retrievals from the Atmospheric InfraRed Sounder(AIRS), the ensemble retrievals RMSE of temperature profiles selected by the PDF was reduced between150 and 320 hPa and below 400 hPa, with a maximum improvement of 0.3 K at 400 hPa. The bias was also reduced in many layers, with a maximum improvement of 0.69 K at 460 hPa. The combined optimal(CombOpt) profile and a mean optimal(MeanOpt) profile of all ensemble physical results were improved below 150 hPa. The MeanOpt profile was better than the CombOpt profile, and was regarded as the final optimal(FinOpt) profile. This study lays the foundation for improving temperature retrievals from hyper-spectral infrared radiance measurements. | ZHANG Jie Zhenglong LI Jun LI Jinglong LI | 2014 | Advances in Atmospheric Sciences2014，31，3： | 2 |
| 10 | A Lipid Droplet-Associated GFP Reporter-Based Screen Identifies New Fat Storage Regulators in C.elegans显示文摘Fat storage disorders including obesity are pandemic human health problems. As a genetically amenable model organism, Caenorhabditis elegans has often been used to explore the molecular mechanisms of fat storage regulation. Dye staining of fixed animals and stimulated Raman scattering(SRS) microscopy methods have been used successfully to study fat storage, but a genetic screening system that takes full advantage of C. elegans transparency to perform live imaging of fluorescent protein reporters has not yet been reported.Here, we investigated the tissue-specific expression of the GFP fusion of Perilipin 1(PLIN1), a Drosophila lipid droplet-associated protein, in C. elegans. Our results indicate that PLIN1::GFP labels lipid droplets and can be used as a fat storage indicator in live worms. Through an RNAi screen, we further identified several previously uncharacterized new fat storage regulators. | Zhenglong Liu Xia Li Qinlan Ge Mei Ding Xun Huang | 2014 | Journal of Genetics and Genomics2014，41，5： | 2 |
| 11 | Identification of critical nodes for cascade faults of grids based on electrical PageRank显示文摘In this paper,the electrical PageRank method is proposed to identify the critical nodes in a power grid considering cascading faults as well as directional weighting.This method can rapidly and accurately focus on the critical nodes in the power system.First,the proposed method simulates the scenario in a grid after a node is attacked by cascading faults.The load loss of the grid is calculated.Second,the electrical PageRank algorithm is proposed.The nodal importance of a grid is determined by considering cascading faults as well as directional weights.The electrical PageRank values of the system nodes are obtained based on the proposed electrical PageRank algorithm and ranked to identify the critical nodes in a grid.Finally,the effectiveness of the proposed method is verified using the IEEE39 node system.The proposed method is highly effective in preventing the occurrence of cascading faults in power systems. | Qingyu Su Cong Chen Zhenglong Sun Jian Li | 2021 | Global Energy Interconnection2021，4，6： | 2 |
| 12 | Added-value of GEO-hyperspectral Infrared Radiances for Local Severe Storm Forecasts Using the Hybrid OSSE Method显示文摘High spectral resolution(or hyperspectral)infrared(IR)sounders onboard low earth orbiting satellites provide high vertical resolution atmospheric information for numerical weather prediction(NWP)models.In contrast,imagers on geostationary(GEO)satellites provide high temporal and spatial resolution which are important for monitoring the moisture associated with severe weather systems,such as rapidly developing local severe storms(LSS).A hyperspectral IR sounder onboard a geostationary satellite would provide four-dimensional atmospheric temperature,moisture,and wind profiles that have both high vertical resolution and high temporal/spatial resolutions.In this work,the added-value from a GEO-hyperspectral IR sounder is studied and discussed using a hybrid Observing System Simulation Experiment(OSSE)method.A hybrid OSSE is distinctively different from the traditional OSSE in that,(a)only future sensors are simulated from the nature run and(b)the forecasts can be evaluated using real observations.This avoids simulating the complicated observation characteristics of the current systems(but not the new proposed system)and allows the impact to be assessed against real observations.The Cross-track Infrared Sounder(CrIS)full spectral resolution(FSR)is assumed to be onboard a GEO for the impact studies,and the GEO CrIS radiances are simulated from the ECMWF Reanalysis v5(ERA5)with the hyperspectral IR all-sky radiative transfer model(HIRTM).The simulated GEO CrIS radiances are validated and the hybrid OSSE system is verified before the impact assessment.Two LSS cases from 2018 and 2019 are selected to evaluate the value-added impacts from the GEO CrIS-FSR data.The impact studies show improved atmospheric temperature,moisture,and precipitation forecasts,along with some improvements in the wind forecasts.An added-value,consisting of an overall 5%Root Mean Square Error(RMSE)reduction,was found when a GEO CrIS-FSR is used in replacement of LEO ones indicat-ing the potential for applications of data from a GEO hyperspectral IR sounder to improve local severe storm forecasts. | Pei WANG Zhenglong LI Jun LI Timothy JSCHMIT | 2021 | Advances in Atmospheric Sciences2021，38，8： | 1 |
| 13 | Experimental Research on Machining Performance of Electrode Materials in Dry EDM 显示文摘 | Liqing Li Zhenglong Wang Yongfeng Guo | 2006 | Materials Science Form2006，，： | 1 |
| 14 | Electricity generation using a baffled microbial fuel cellconvenient for stacking显示文摘 | Li Zhenglong Yao Lu Kong Lingcai | 2008 | Bioresource Technology2008，99，6： | 1 |
| 15 | Experimental study on welding characteristics of CO2 laser TIG hybrid welding process显示文摘 | CHEN Yanbin LEI Zhenglong LI Liqun | 2006 | Science and Technology of Welding and Joining2006，11，4： | 1 |
| 16 | Origin of crude oils in the Jinhu Depression of North Jiangsu-South Yellow Sea Basin, eastern China显示文摘 | Xiongqi Pang Maowen Li Sumei Li Zhijun Jin Zhenglong Xu Anding Chen | 2003 | Organic Geochemistry2003，，4： | 1 |
| 17 | Molecular cloning, functional analysis and localization of a novel gene encoding polygalacturonase-inhibiting protein in Chorispora bungeana显示文摘 | Cuixia Di Ming Li Feng Long Muqun Bai Yajie liu Xiaolin Zheng Shijian Xu Yun Xiang Zhenglong Sun Lizhe An | 2009 | Planta2009，，1： | 1 |
| 18 | Experimental study up-on welding characteristics of CO2 laser-TIG hybrid welding process显示文摘 | Chen Yanbin Lei Zhenglong Li Liqun | 2006 | Science and Technology of Welding and Joining2006，11，4： | 1 |
| 19 | High-fidelity structured illumination microscopy by point-spread-function engineering显示文摘Structured illumination microscopy(SIM)has become a widely used tool for insight into biomedical challenges due to its rapid,long-term,and super-resolution(SR)imaging.However,artifacts that often appear in SIM images have long brought into question its fidelity,and might cause misinterpretation of biological structures.We present HiFi-SIM,a high-fidelity SIM reconstruction algorithm,by engineering the effective point spread function(PSF)into an ideal form.HiFi-SIM can effectively reduce commonly seen artifacts without loss of fine structures and improve the axial sectioning for samples with strong background.In particular,HiFi-SIM is not sensitive to the commonly used PSF and reconstruction parameters;hence,it lowers the requirements for dedicated PSF calibration and complicated parameter adjustment,thus promoting SIM as a daily imaging tool. | Gang Wen Simin Li Unbo Wang Xiaohu Chen Zhenglong Sun Yong Liang Xin Jin Yifan Xing Yaming jiu Yuguo Tang Hui Li | 2021 | Light(Science & Applications)2021，10，5： | 1 |
| 20 | Comparison between current and future environmental satellite imagers on cloud classification using MODIS显示文摘 | Li Zhenglong Li Jun MENZEL W P | 2007 | Remote Sensing Environment2007，108，3： | 1 |
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