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| 1 | Can acupuncture therapy reduce preoperative anxiety?A systematic review and meta-analysis显示文摘Background:Acupuncture therapy has shown promise for effectively relieving preoperative anxiety.Nevertheless,previous findings from randomized controlled trials(RCTs)are inconsistent and must be examined in detail.Objective:This study systematically evaluates the efficacy and safety of acupuncture therapy for preoperative anxiety as well as the quality of evidence supporting this application.Search strategy:The China National Knowledge Infrastructure Database,Wanfang Data Journal Database,Chinese Biomedical Literature Database,Chongqing VIP,Embase,Pub Med and Cochrane Library Databases were queried from their inception to 19,February 2020,using keywords such as“acupuncture therapy,”“preoperative”and“anxioty.”Manual searches expanded the search breadth and included conference abstracts and other reference lists.Inclusion criteria:RCTs were included in the current study if they contained a comparison between a group of anxiety patients that received acupuncture therapy and a control group that received sham acupuncture.Data extraction and analysis:Literature was reviewed,and various articles were selected using the Note Express 3.2.0 software.Two researchers independently screened and extracted data and evaluated the risk of bias in the included studies.The Rev Man 5.3 software was used for data aggregation and the Grades of Recommendation,Assessment,Development and Evaluation(GRADE)assessment was used to evaluate the quality of the study outcomes.Results:Twelve studies were included in the review,containing a total of 916 patients.Meta-analysis showed that,compared with the control group,patients who received acupuncture therapy had reduced State-Trait Anxiety Inventory Scale(STAI-S)score(mean difference[MD]=–9.07,95%confidence interval[CI][–13.19 to–4.96],P<0.0001)and Visual Analogue Scale(VAS)score(MD=–1.37,95%CI[–2.29 to–0.45],P=0.003).However,for the Hamilton Anxiety Scale(HAMA)score,there was no difference between the two groups(MD=–3.98,95%CI[–12.89 to 4.92],P=0.38).Further,the GRADE assessment demonstrated that the STAI-S was of moderate quality,the VAS of low quality and the HAMA of very low quality.Conclusion:Acupuncture therapy may be able to decrease anxiety in preoperative patients,but the results need to be further verified due to the small sample sizes and the low quality of evidence to date. | Qiu-yu Tong Ran Liu Kun Zhang Yuan G ao Guang-wei Cui Wei-dong Shen | 2021 | Journal of Integrative Medicine2021，19，1： | 15 |
| 2 | Knockdown of OsHox33, a member of the class III homeodomain-leucine zipper gene family, accelerates leaf senescence in rice显示文摘The class III homeodomain-leucine zipper(HD-Zip III)gene family plays important roles in plant growth and development,including regulation of apical embryo patterning,embryonic shoot meristem formation,leaf polarity,vascular development,and meristem function,with a particularly crucial function in leaf development.Although HD-Zip III members are highly conserved in land plants,previous studies,such as genetic analyses based on multiple mutants in Arabidopsis and other plants,suggest that various HD-Zip III family genes have evolved with distinct functions and pleiotropic effects on plant growth and development.In this study,we analyzed a HD-Zip III member,OsHox33,and demonstrated that it plays an important role in age-dependent leaf senescence in rice.We constructed two specific RNAi vectors derived from the 5′-end region and 3′-UTR of OsHox33 to knockdown its expression.Transgenic plants harboring either RNAi construct displayed similar phenotypes of precocious leaf senescence symptoms,suggesting that knockdown of OsHox33 accelerates leaf senescence in rice.pOsHox33::GUS fusion expression and RT-PCR revealed that OsHox33 is highly expressed in young organs,especially in young meristems such as shoot apical meristems,intercalary meristems,and young callus.In addition,real-time PCR indicated that OsHox33 was more highly expressed in young leaves than in old leaves.To further investigate OsHox33 function,we analyzed chloroplast ultrastructure in different-aged leaves of RNAi plants,and found that OsHox33 knockdown accelerated chloroplast degradation,which is consistent with RNAi phenotypes.Finally,real-time PCR studies showed that OsHox33 can regulate the expression of GS1 and GS2,two senescence-associated genes.Taken together,the work presented here provides new insights into the function of HD-Zip III members in plants. | LUAN WeiJiang SHEN Ao JIN ZhiPing SONG SuSheng LI ZhengLong SHA AiHua | 2013 | Science China(Life Sciences)2013，56，12： | 13 |
| 3 | Evaluation of biodiesel from Xanthoceras sorbifolia Bunge seed kernel oil from 13 areas in China显示文摘Oil content from seed kernels of Xanthoceras sorbifolia from 13 areas in China was analyzed by gas chromatography–mass spectrometry to determine oil characteristics and biodiesel properties. The seeds had a high kernel percentage (53.67%± 7.51), oil content (52.21%± 4.01), and biodiesel yield (99.77%± 0.21). Among the fatty acids in the oil were high percentages of linoleic acid (41.66 ± 2.26)% and oleic acid (28.44%± 2.03). Most of the fuel properties complied well with the ASTM D6751-10, EN 14214-08, and GB/T 20828-2014 standards. The 13 sampling areas were grouped into four clusters based on different kernel percentage, oil content, biodiesel yield, and fatty acid composition. The results showed that the quality of kernel oils from seeds from Ar Horqin Banner was the best, although kernel oils from seeds in all 13 areas were suitable for biodiesel production. This study provides a basis for selecting optimal sites to harvest seeds from X. sorbifolia. | Zhan Shen Kaiquan Zhang Yan Ao Luyi Ma Jie Duan | 2019 | Journal of Forestry Research2019，30，3： | 7 |
| 4 | Extractive distillation: Advances in conceptual design, solvent selection,and separation strategies显示文摘Extractive distillation(ED) is one of the most promising approaches for the separation of the azeotropic or closeboiling mixtures in the chemical industry. The purpose of this paper is to provide a broad overview of the recent development of key aspects in the ED process involving conceptual design, solvent selection, and separation strategies. To obtain the minimum entrainer feed flow rate and reflux ratio for the ED process, the conceptual design of azeotropic mixture separation based on a topological analysis via thermodynamic feasibility insights involving residue curve maps, univolatility lines, and unidistribution curves is presented. The method is applicable to arbitrary multicomponent mixtures and allows direct screening of design alternatives. The determination of a suitable solvent is one of the key steps to ensure an effective and economical ED process. Candidate entrainers can be obtained from heuristics or literature studies while computer aided molecular design(CAMD) has superiority in efficiency and reliability. To achieve optimized extractive distillation systems, a brief review of evaluation method for both entrainer design and selection through CAMD is presented. Extractive distillation can be operated either in continuous extractive distillation(CED) or batch extractive distillation(BED), and both modes have been well-studied depending on the advantages in flexibility and low capital costs. To improve the energy efficiency, several configurations and technological alternatives can be used for both CED and BED depending on strategies and main azeotropic feeds. The challenge and chance of the further ED development involving screening the best potential solvents and exploring the energy-intensive separation strategies are discussed aiming at promoting the industrial application of this environmentally friendly separation technique. | Shirui Sun Liping Lü Ao Yang Shun'an Wei Weifeng Shen | 2019 | Chinese Journal of Chemical Engineering2019，27，6： | 6 |
| 5 | Amyloid beta-peptide worsens cognitive impairment following cerebral ischemia-reperfusion injury显示文摘Amyloid β-peptide,a major component of senile plaques in Alzheimer’s disease,has been implicated in neuronal cell death and cognitive impairment.Recently,studies have shown that the pathogenesis of cerebral ischemia is closely linked with Alzheimer’s disease.In this study,a rat model of global cerebral ischemia-reperfusion injury was established via occlusion of four arteries;meanwhile,fibrillar amyloidβ-peptide was injected into the rat lateral ventricle.The Morris water maze test and histological staining revealed that administration of amyloidβ-peptide could further aggravate impairments to learning and memory and neuronal cell death in the hippocampus of rats subjected to cerebral ischemia-reperfusion injury.Western blot showed that phosphorylation of tau protein and the activity of glycogen synthase kinase 3βwere significantly stronger in cerebral ischemia-reperfusion injury rats subjected to amyloid β-peptide administration than those undergoing cerebral ischemia-reperfusion or amyloidβ-peptide administration alone.Conversely,the activity of protein phosphatase 2A was remarkably reduced in rats with cerebral ischemia-reperfusion injury following amyloidβ-peptide administration.These findings suggest that amyloidβ-peptide can potentiate tau phosphorylation induced by cerebral ischemia-reperfusion and thereby aggravate cognitive impairment. | Bo Song Qiang Ao Ying Niu Qin Shen Huancong Zuo Xiufang Zhang Yandao Gong | 2013 | Neural Regeneration Research2013，8，26： | 5 |
| 6 | A genetic diversity assessment of starch quality traits in rice landraces from the Taihu basin,China显示文摘There are nearly 1 000 rice landrace varieties in the Taihu basin, China. To assess the genetic diversity of the rice, 24 intragenic molecular markers(representing 17 starch synthesis-related genes) were investigated in 115 Taihu basin rice landraces and 87 improved cultivars simultaneously. The results show that the average genetic diversity and polymorphism information content values of the landraces were higher than those of improved cultivars. In total, 41 and 39 allele combinations(of the 17 genes) were derived from the landraces and improved cultivars, respectively; only two identical allele combinations were found bet ween the two rice variety sources. Cluster analysis, based on the molecular markers, revealed that the rice varieties could be subdivided into five groups and, within these, the japonica improved rice and japonica landrace rice varieties were in two separate groups. According to the quality reference criteria to classify the rice into grades, some of the landraces were found to perform we ll, in terms of starch quality. For example, according to NY /T595-2002 criteria from the Ministry of Agriculture of China, 25 and 33 landraces reached grade 1, in terms of their apparent amylose content and gel consistency. Th e varieties that had outstanding quality could be used as breeding materials for rice quality breeding programs in the future. Our study is useful for future applications, such as genetic diversity studies, the protection of rice variety and improvment of rice quality in breeding programs. | AO Yan XU Yong CUI Xiao-fen WANG An TENG Fei SHEN Li-qun LIU Qiao-quan | 2016 | Journal of Integrative Agriculture2016，15，3： | 5 |
| 7 | Myofiber necroptosis promotes muscle stem cell proliferation via releasing Tenascin-C during regeneration显示文摘Necroptosis,a form of programmed cell death,is characterized by the loss of membrane integrity and release of intracellular contents,the execution of which depends on the membrane-disrupting activity of the Mixed Lineage Kinase Domain-Like protein(MLKL)upon its phosphorylation.Here we found myofibers committed MLKL-dependent necroptosis after muscle injury.Either pharmacological inhibition of the necroptosis upstream kinase Receptor Interacting Protein Kinases 1(RIPK1)or genetic ablation of MLKL expression in myofibers led to significant muscle regeneration defects.By releasing factors into the muscle stem cell(MuSC)microenvironment,necroptotic myofibers facilitated muscle regeneration.Tenascin-C(TNC),released by necroptotic myofibers,was found to be critical for MuSC proliferation.The temporary expression of TNC in myofibers is tightly controlled by necroptosis;the extracellular release of TNC depends on necroptotic membrane rupture.TNC directly activated EGF receptor(EGFR)signaling pathway in MuSCs through its N-terminus assembly domain together with the EGF-like domain.These findings indicate that necroptosis plays a key role in promoting MuSC proliferation to facilitate muscle regeneration. | Shen'ao Zhou Wei Zhang Gaihong Cai Yingzhe Ding Caixia Wei Sheng Li Yu Yang Jie Qin Dan Liu Hao Zhang Xiexiang Shao Jianhua Wang Hongye Wang Wenjun Yang Huating Wang She Chen Ping Hu Liming Sun | 2020 | Cell Research2020，30，12： | 4 |
| 8 | Umbilical Cord Blood Lead Levels in Shanghai, China显示文摘This study was designed to determine the cord blood lead (BPb) levels of babies born in one urban area of Shanghai, and to preliminarily identify the demographic, social environment and prenatal factors which have an effect on the cord BPb concentrations. From August to November 1993, umbilical cord blood samples were obtained from 605 live newborns in the Yangpu Maternal and Child Hospital. 257 samples were excluded from measurement because of clotting. In 348 cord samples, the geometric mean of cord BPb levels was 9. 2μg/dl, with a 95 % confidence interval of the mean 8. 86-9. 54 (μg/dl). 142 babies (40. 8 % ) had cord BPb levels of 10μg/dl or greater. As a result of this high percentage of newborns with BPb levels equal to or greater than 10 μg/dl, we estimate that each year in the Shanghal City about 60,000 newborns are at risk for developing neuropsychological deficiencies caused by maternal lead exposure during pregnancy. To investigate the factors affecting cord blood levels, the subjects with levels greater than the 70th percentile (10. 7μg/dl) (n = 104) and less than the 3oth percentile (7. 4μg/dl) (n = 104) were selected to compare the demographic, environ ment and prenatal medical history. Increased BPb levels at birth were associated with maternal passive smoking, a family member being occupationally exposed to lead, proximity to major traffic way, household coal combustion, neighborhood coal combustion, low level of meternal occupations, and the increasing occurrence of having the high lead foodstuff pidan (preserved duck egg) during pregnancy. We conclude that prenatal lead exposure has become an impor tant health issue for young children in | SHEN XIAO-MING YAN CHONG-HUAI GUO DI WU SHENG-MEI LI REN-QIU HUANG HONG AO LI-MING ZHOU JIAN-DE HONG ZHAO-YI XU JI-DE JIN XING-MING AND TANG JUN-MING (Division of Childhood Lead Poisoning Prevention, Shanghai Institute for Pediatric Rese | 1997 | Biomedical and Environmental Sciences1997，10，1： | 4 |
| 9 | Reverse Genetic Analysis of Transcription Factor Os Hox9, a Member of Homeobox Family, in Rice显示文摘Homeobox transcription factors participate in the growth and development of plants by regulating cell differentiation, morphogenesis and environmental signal response. To reveal the functions of these transcription factors in rice, we constructed the RNAi vectors of Os Hox9, a member of homeobox family, and analyzed the function of Os Hox9 using reverse genetics. The plant height and tillering number of RNAi transgenic plants decreased compared with those of wild-type plants. Reverse transcription-polymerase chain reaction analysis showed that Os Hox9 expression reduced in the transgenic plants with phenotypic variance, whereas that in the transgenic plants without phenotypic variance was similar to that in the wild-type plants. This result suggests that the phenotypes of the transgenic plants were caused by RNAi effects. The tissue-specificity of Os Hox9 expression indicated that it was expressed in different organs, with high expression in stem apical meristem and young panicles. Subcellular location of Os Hox9 demonstrated that it was localized on the cell membrane. | AI Li-ping SHEN Ao GAO Zhi-chao LI Zheng-long SUN Qiong-lin LI Ying-ying LUAN Wei-jiang | 2014 | Rice science2014，21，6： | 4 |
| 10 | Effects of ketamine on mouse hippocampal inflammatory cytokines IL-6,IL-1β and TNF-α levels in acute and chronic administration models显示文摘OBJECTIVE Ketamine is an injectable anesthetic and recreational drug of abuse commonly used worldwide. Many experimental studies have shown that ketamine can impair cognitive function and induce psychotic states. Neuroinflammation has been suggested to play an important role in neurodegeneration. Meanwhile,ketamine has been showed to modulate the levels of inflammatory cytokines.Therefore,we sought to investigate whether the effects of ketamine on the central nervous system is associated with the inflammatory cytokines. METHODS We established acute(single or multiple intraperitoneal injection) and chronic(six months daily intraperitoneal injection) ketamine administration models in C57BL/6 mice,evaluated the spatial recognition memory and emotional response by applying the Y maze test and open field test. We analyzed the changes of inflammatory cytokines interleukin-6(IL-6),interleukin-1β(IL-1β) and tumor necrosis factor-α(TNF-α) levels in mouse hippocampus,employing Western blot,quantitative reverse transcriptase-polymerase chain reaction(qR T-PCR) and immunohistochemistry. RESULTS Ketamine induced spatial recognition memory deficit,reduced anxiety-like behaviors in mice after chronic administration,and it was dose-dependent. Moreover,we found that ketamine could increase the levels of mouse hippocampal inflammatory cytokines IL-6 and IL-1β after single,multiple and long-term administration in a dose-dependent manner. However,the level of TNF-α expressed differently in mouse hippocampus under different conditions. Single administration of ketamine increased the level of TNF-α,whereas multiple and long-term administration decreased it significantly. We considered that TNF-α might exist bi-directional regulatory pathway,which was associated with the dose and duration of ketamine administration. CONCLUSION Our results suggest that the alterations of inflammatory cytokines IL-6,IL-1β and TNF-α levels may be involved in the neurotoxicity of ketamine. | LI Yan-ning SHEN Rui-peng WEN Ge-hua DING Run-tao DU Ao ZHOU Ji-chuan DONG Zhi-bin REN Xing-hua YAO Hui ZHAO Rui ZHANG Guo-hua LU Yan WU Xu | 2017 | 中国药理学与毒理学杂志2017，31，5： | 3 |
| 11 | Tribological Behavior of TiAl-Multilayer Graphene-Ag Composites at Different Temperatures and Sliding Speeds显示文摘Tribological behavior of TiAl-multilayer graphene-Ag composites(TMACs) prepared by spark plasma sintering against a Si_3N_4 ball was investigated on a ball-on-disk high-temperature tribometer at different test temperatures and sliding speeds in this study.The test results showed that TMACs had the lower friction coefficient and less wear rate at450 ℃-0.25 m/s,which was attributed to the formation of the high-strength and intact tribofilms on worn surface.At450 ℃-0.25 m/s,during the sliding process,multilayer graphene(MLG) was ground out to form the high-strength skeletons on the worn surface of TMACs.Ag was migrated from the worn surface and combined with the MLG skeleton to form the high-strength and intact tribofilms.The high-strength and intact tribofilms were beneficial to lowering the friction coefficient for the lubricating effect of Ag and decreasing wear rate for the enhancing effect of MLG skeleton. | Jia-Liang Zou Xiao-Liang Shi Qiao Shen Kang Yang Yu-Chun Huang Ao Zhang Yu-Fu Wang Qiao-Xin Zhang | 2017 | Acta Metallurgica Sinica(English Letters)2017，30，3： | 3 |
| 12 | SOMCL-085, a novel multi-targeted FGFR inhibitor, displays potent anticancer activity in FGFR-addicted human cancer models显示文摘异常成纤维细胞生长因素受体(FGFR ) 激活越过恶意的一个多样的系列被发现,特别缺乏有效治疗的那些。SOMCL-085 是一个新奇 FGFR 主导的多目标 kinase 禁止者。这里,我们在 vitro 并且在 vivo 探索了 SOMCL-085 的指向 FGFR anticancer 活动。在屏蔽的 20 酷氨酸 kinases 的一块面板之中, SOMCL-085 potently 分别地禁止了 FGFR1, FGFR2 和 FGFR3 kinase 活动,与 1.8, 1.9 和 6.9 nmol/L 的 IC 50 价值。这混合物同时禁止了 angiogenesis kinases VEGFR 和 PDGFR,但是没有另外的 12 酷氨酸 kinases 上的明显的禁止的效果。在有测试的 FGFR 激活的不同机制的 3 根代表性的人的癌症房间线, SOMCL-085 (20-500 nmol/L ) dose-dependently 禁止了 FGFR1-3 phosphorylation 和他们的关键下游的受动器 PLC 和英皇家空军之阶级最低之兵的 phosphorylation。在 7 根 FGFR 异常人的癌症房间线,不管 FGFR 在激活上的机械学的复杂性, SOMCL-085 potently 由在 G 1/S 阶段逮捕房间禁止了驾驶 FGFR 的房间增长。在 放大FGFR1 的肺癌症房间线 H1581 异种皮移植老鼠和 放大FGFR2 的胃的癌症房间衬里 SNU16 异种皮移植老鼠, SOMCL-085 的口头的管理( 25 ,吗 50 mg ????????????????????????????????漠敬湡汯捩??儠?????畱湩硯污湩?? | Xi-fei JIANG Yang DAI Xia PENG Yan-yan SHEN Yi SU Man-man WEI Wei-ren LIU Zhen-bin DING Ao ZHANG Ying-hong SHI Jing AI | 2018 | Acta Pharmacologica Sinica2018，39，2： | 3 |
| 13 | Energy-efficient recovery of tetrahydrofuran and ethyl acetate by triple-column extractive distillation:entrainer design and process optimization显示文摘An energy-efficient triple-column extractive distillation process is developed for recovering tetrahydrofuran and ethyl acetate from industrial effluent.The process development follows a rigorous hierarchical design procedure that involves entrainer design,thermodynamic analysis,process design and optimization,and heat integration.The computer-aided molecular design method is firstly used to find promising entrainer candidates and the best one is determined via rigorous thermodynamic analysis.Subsequently,the direct and indirect triple-column extractive distillation processes are proposed in the conceptual design step.These two extractive distillation processes are then optimized by employing an improved genetic algorithm.Finally,heat integration is performed to further reduce the process energy consumption.The results indicate that the indirect extractive distillation process with heat integration shows the highest performance in terms of the process economics. | Ao Yang Yang Su Tao Shi Jingzheng Ren Weifeng Shen Teng Zhou | 2022 | Frontiers of Chemical Science and Engineering2022，16，2： | 3 |
| 14 | Relationship between deep-sea fish distributions and oceanic conditions of the East China Sea显示文摘Relationshipbetweendeep-seafishdistributionsandoceanicconditionsoftheEastChinaSea￥ShenJin'ao(ReceivedNovember17,1993;accepted... | Shen Jin’ao ( Department of Fishery Engineering, Shanghai Fisheries University, 334 Jungong Road, Shanghai 200090, China) | 1994 | Acta Oceanologica Sinica1994，13，4： | 3 |
| 15 | Effect of Planting Density,Fertilizer Level,and Number of Seedlings per Hole on Yield of Coix Lachryrma-jobi L.显示文摘In order to study high yield cultivation technology for Coix Lachryrma-jobi L.,and discuss the effect of planting density,fertilizer level,and number of seedlings per hole on yield of Coix Lachryrma-jobi L.,this paper took domesticated variety Xingren Small White Shell of Coix Lachryrma-jobi L. as an example. Using L9( 34) orthogonal design,it selected optimum combination of planting density,fertilizer level,and number of seedlings per hole to guide planting of Coix Lachryrma-jobi L.. Experiment indicated that the optimum planting density( spacing in the rows and spacing between rows) of Coix Lachryrma-jobi L. is 40 cm x 80 cm; the optimum fertilizer level is pure nitrogen 15 kg,pure phosphorus 15 kg and pure potassium 15 kg per mu; the optimum number of seedlings per hole is 5 seedlings per hole. Through variance analysis,it indicated that planting density exerts significance effect on the yield of Coix Lachryrma-jobi L. | Zhiqin SONG Maohong AO Gang SHEN Pengfei LIU | 2015 | Asian Agricultural Research2015，7，8： | 3 |
| 16 | Exhausted local lactate accumulation via injectable nanozyme-functionalized hydrogel microsphere for inflammation relief and tissue regeneration显示文摘Local lactate accumulation greatly hinders tissue repair and regeneration under ischemic condition.Herein,an injectable microsphere(MS@MCL)for local lactate exhaustion was constructed by grafting manganese dioxide(MnO_(2))-lactate oxidase(LOX)composite nanozyme on microfluidic hyaluronic acid methacrylate(HAMA)microspheres via chemical bonds,achieving a long-term oxygen-promoted lactate exhaustion effect and a long half-life in vivo.The uniform and porous microspheres synthesized by microfluidic technology is beneficial to in situ injection therapy and improving encapsulation efficiency.Furthermore,chemical grafting into HAMA microspheres through amide reactions promoted local enzymatic concentration and activity enhancement.It was showed that the MS@MCL eliminated oxidative and inflammatory stress and promoted extracellular matrix metabolism and cell survival when co-cultured with nucleus pulposus cells(NPCs)in vitro.In the rat degenerative intervertebral disc model caused by lactate injection,MS@MCL showed a long-term therapeutic effect in reducing intervertebral height narrowing and preventing extracellular matrix(ECM)degradation as well as inflammatory damage in vivo.Altogether,this study confirms that this nanozyme-functionalized injectable MS@MCL effectively improves the regenerative and reparative effect in ischemic tissues by disposing of enriched lactate in local microenvironment. | Jieliang Shen Ao Chen Zhengwei Cai Zhijie Chen Ruichao Cao Zongchao Liu Yuling Li Jie Hao | 2022 | Bioactive Materials2022，7，6： | 2 |
| 17 | Combining 53BP1 with BRCA1 as a biomarker to predict the sensitivity of poly(ADP-ribose) polymerase (PARP) inhibitors显示文摘别对治疗在有 BRCA1 缺乏的癌症的病人的一半上作出回应与 poly (自动数据处理核糖) 聚合酶(PARP ) 禁止者。在这研究,我们报导 53BP1 和 BRCA1 的联合可以用作 PARP 禁止者敏感的 biomarker。基于四个相应再结合修理(HR ) 的 mRNA 层次基因和 PARP 禁止者敏感,我们选择了 BRCA1 缺乏的 MDA-MB-436 房间进行 RNA 干扰。减少 53BP1,然而并非另外的三 HR 基因的表示,被发现降低 simmiparib 敏感。另外,我们产生了 53BP1 //BRCA1/ 由抄写的同种细胞的变体像使活跃之物的受动器核酸酶(TALEN ) 技术并且发现那弄空的 53BP1 随他们的 IC 50 价值的 36.7 褶层增加损害了 PARP 禁止者敏感。与它 PARP 禁止者敏感上的效果一致, 53BP1 损失减轻了房间周期拘捕和 apoptosis 并且部分恢复了 HR 功能。重要地, 53BP1 弄空戏剧性地减少了 PARP 禁止者的能力在 vivo 压制肿瘤生长。simmiparib 的抑制率为 BRCA1 缺乏的 MDA-MB-436 异种皮移植是 74.16% ,但是仅仅 7.79% 为 53BP1/BRCA1-deficient 异种皮移植。在双缺乏的房间的重新表示的 53BP1 恢复了 PARP 禁止者敏感和 HR 管理者的层次。认为至少 10%BRCA1 缺乏的胸和卵巢的癌症减少了 53BP1 的表示,用有象为耐心的选择的 biomarker 的 BRCA1 的 53BP1 的联合应该减少与 PARP 禁止者经历无益的治疗的病人的数字。 | Zhong-min YANG Xue-mei LIAO Yi CHEN Yan-yan SHEN Xin-ying YANG Yi SU Yi-ming SUN Ying-lei GAO Jian DING Ao ZHANG Jin-xue HE Ze-hong MIAO | 2017 | Acta Pharmacologica Sinica2017，38，7： | 2 |
| 18 | A time domain correction method for Doppler-distortion signal from acoustic moving source显示文摘Doppler effect widely exists in the signal from the moving acoustic source.In order to solve such problems as frequency shift and frequency band expansion,a time domain correction method is presented in this paper.First,the discrete time vector for interpolation and the amplitude restoration formula is derived based on the moving relationship and the Morse acoustic theory,then the amplitude weights are corrected and the distortion signal is interpolated.Every point of the discrete signal is operated separately in time domain.Compared with the existing frequency domain methods,this method does not need to know the characteristic frequency beforehand and would not be influenced by the blending of the frequency band.Hence,this method can be employed to correct multiple frequency signals and it is also a simple and effective Doppler effect reduction method. | LIU Fang HE Qingbo SHEN Changqing HU Fei ZHANG Ao KONG Fanrang | 2014 | Chinese Journal of Acoustics2014，33，1： | 2 |
| 19 | Exosomes secreted from cardiomyocytes suppress the sensitivity of tumor ferroptosis in ischemic heart failure显示文摘Heart failure(HF)patients in general have a higher risk of developing cancer.Several animal studies have indicated that cardiac remodeling and HF remarkably accelerate tumor progression,highlighting a cause-and-effect relationship between these two disease entities.Targeting ferroptosis,a prevailing form of non-apoptotic cell death,has been considered a promising therapeutic strategy for human cancers.Exosomes critically contribute to proximal and distant organ-organ communications and play crucial roles in regulating diseases in a paracrine manner.However,whether exosomes control the sensitivity of cancer to ferroptosis via regulating the cardiomyocyte-tumor cell crosstalk in ischemic HF has not yet been explored.Here,we demonstrate that myocardial infarction(MI)decreased the sensitivity of cancer cells to the canonical ferroptosis activator erastin or imidazole ketone erastin in a mouse model of xenograft tumor.Post-MI plasma exosomes potently blunted the sensitivity of tumor cells to ferroptosis inducers both in vitro in mouse Lewis lung carcinoma cell line LLC and osteosarcoma cell line K7M2 and in vivo with xenograft tumorigenesis model.The expression of miR-22-3p in cardiomyocytes and plasma-exosomes was significantly upregulated in the failing hearts of mice with chronic MI and of HF patients as well.Incubation of tumor cells with the exosomes isolated from post-MI mouse plasma or overexpression of miR-22-3p alone abrogated erastin-induced ferroptotic cell death in vitro.Cardiomyocyte-enriched miR-22-3p was packaged in exosomes and transferred into tumor cells.Inhibition of cardiomyocyte-specific miR-22-3p by AAV9 sponge increased the sensitivity of cancer cells to ferroptosis.ACSL4,a pro-ferroptotic gene,was experimentally established as a target of miR-22-3p in tumor cells.Taken together,our findings uncovered for the first time that MI suppresses erastin-induced ferroptosis through releasing miR-22-3p-enriched exosomes derived from cardiomyocytes.Therefore,targeting exosome-mediated cardiomyocyte/tumor pathological communication may offer a novel approach for the ferroptosis-based antitumor therapy. | Ye Yuan Zhongting Mei Zhezhe Qu Guanghui Li Shuting Yu Yingqi Liu Kuiwu Liu Zhihua Shen Jiaying Pu Yanquan Wang Changhao Wang Zhiyong Sun Qian Liu Xiaochen Pang Ao Wang Zijing Ren Tong Wang Ying Liu Jinhuan Hong Jiajie Xie Xin Li Zhonghua Wang Weijie Du Baofeng Yang | 2023 | Signal Transduction and Targeted Therapy2023，8，4： | 2 |
| 20 | Phosphorylation of tau protein over time in rats subjected to transient brain ischemia显示文摘Transient brain ischemia has been shown to induce hyperphosphorylation of the microtubule-associated protein tau.To further determine the mechanisms underlying these processes,we investigated the interaction between tau,glycogen synthase kinase(GSK)-3βand protein phosphatase 2A.The results confirmed that tau protein was dephosphorylated during brain ischemia;in addition,the activity of GSK-3βwas increased and the activity of protein phosphatase2A was decreased.After reperfusion,tau protein was hyperphosphorylated,the activity of GSK-3βwas decreased and the activity of protein phosphatase 2A remained low.Importantly,the interaction of tau with GSK-3βand protein phosphatase 2A was altered during ischemia and reperfusion Lithium chloride could affect tau phosphorylation by regulating the interaction of tau with GSK-3βand protein phosphatase 2A,and improve learning and memory ability of rats after transient brain ischemia.The present study demonstrated that it was the interaction of tau with GSK-3βand protein phosphatase 2A,rather than their individual activities,that dominates the phosphorylation of tau in transient brain ischemia.Hyperphosphorylated tau protein may play an important role in the evolution of brain injury in ischemic stroke.The neuroprotective effects of lithium chloride partly depend on the inhibition of tau phosphorylation during transient brain ischemia. | Bo Song Qiang Ao Zhen Wang Weiqiang Liu Ying Niu Qin Shen Huancong Zuo Xiufang Zhang Yandao Gong | 2013 | Neural Regeneration Research2013，8，34： | 2 |
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