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| 1 | Structural and biochemical mechanism responsible for the stay-green phenotype in common wheat显示文摘In order to increase the supply of assimilated carbon to grain, a new stay-green wheat cultivar, Chuannong17 (CN17), with delayed leaf senescence, carrying wheat-rye 1RS.1BL translo- cated chromosome was developed. CN17 exhibited distinct differences in net photosynthetic rate (Pn), chlorophyll (Chl) content, malondialdehyde (MDA) content, activity of both superoxide dismutase (SOD) and catalase (CAT) during the grain filling stage, and flag leaf senescence compared with the control. The new cultivar maintained longer and higher photosyn- thetic competence compared with the control, and this aspect correlated with the difference in chloro-plast development. Moreover, the stay-green pheno-type of CN17 was also observed under natural growth conditions. Consequently, the coordination of the physiological, biochemical, and structural aspects in the stay-green cultivar produced higher seed weights and per-plant yield compared with the control cultivar. | LUO Peigao REN Zhenglong WU Xianhua ZHANG Huaiyu ZHANG Huaiqiong FENG Juan | 2006 | Chinese Science Bulletin2006，51，21： | 9 |
| 2 | Analysis of DNA methylation variation in wheat genetic background after alien chromatin introduction based on methylation-sensitive amplification polymorphism显示文摘During the process of alien germplasm introduced into wheat genome by chromosome engineering, extensive genetic variations of genome structure and gene expression in recipient could be induced. In this study, we performed GISH (genome in situ hybridization) and AFLP (amplified fragment length po-lymorphism) on wheat-rye chromosome translocation lines and their parents to detect the identity in genomic structure of different translocation lines. The results showed that the genome primary struc-ture variations were not obviously detected in different translocation lines except the same 1RS chromosome translocation. Methylation sensitive amplification polymorphism (MSAP) analyses on genomic DNA showed that the ratios of fully-methylated sites were significantly increased in translo-cation lines (CN12, 20.15%; CN17, 20.91%; CN18, 22.42%), but the ratios of hemimethylated sites were significantly lowered (CN12, 21.41%; CN17, 23.43%; CN18, 22.42%), whereas 16.37% were fully-me-thylated and 25.44% were hemimethylated in case of their wheat parent. Twenty-nine classes of me-thylation patterns were identified in a comparative assay of cytosine methylation patterns between wheat-rye translocation lines and their wheat parent, including 13 hypermethylation patterns (33.74%), 9 demethylation patterns (22.76%) and 7 uncertain patterns (4.07%). In further sequence analysis, the alterations of methylation pattern affected both repetitive DNA sequences, such as retrotransposons and tandem repetitive sequences, and low-copy DNA. | ZHANG Yong LIU ZhaoHui LIU Cheng YANG ZuJun DENG KeJun PENG JinHua ZHOU JianPing LI GuangRong TANG ZongXiang REN ZhengLong | 2008 | Chinese Science Bulletin2008，53，1： | 8 |
| 3 | Haplotype variation of Green Revolution gene Rht-D1 during wheat domestication and improvement显示文摘Green Revolution made a substantial contribution to wheat yields worldwide in the 1960 s and 1970 s. It is of great importance to analyze the haplotype variation of Rht-D1, the Green Revolution gene, during wheat(Triticum aestivum L.)domestication and breeding to understand its evolution and function in wheat breeding history. In this study, the Rht-D1 and its flanking regions were sequenced and single nucleotide polymorphisms were detected based on a panel of 45 accessions of Aegilops tauschii, 51 accessions of landraces and 80 accessions of commercial varieties. Genetic diversity in the wild accessions was much higher than that in the varieties and higher than that reported previously. Seven haplotypes(Hapl I to Hapl VII) of Rht-D1 were identified and their evolutionary relationships were proposed. In addition to the well-known Green Revolution allele Rht-D1b, Hapl VII(an allele Rht-D1k) was identified in early breeding varieties, which reduced plant height by 16%. The results suggested that Rht-D1k had been used in breeding before the Green Revolution and made a great contribution to wheat production worldwide.Based on the breeding history and molecular evidence, we proposed that the wheat Green Revolution in China and International Maize and Wheat Improvement Center(CIMMYT)occurred independently. | Chihong Zhang Lifeng Gao Jiaqiang Sun Jizeng Jia Zhenglong Ren | 2014 | Journal of Integrative Plant Biology2014，56，8： | 5 |
| 4 | Genes for resistance to stripe rust on chromosome 2B and their application in wheat breeding显示文摘Stripe rust,caused by Puccinia striiformis f.sp.tritici,is one of the most damaging diseases of wheat worldwide.Growing resistant cultivars is the most economic and environmental friendly way to control the disease.There are many resistance genes to stripe rust located on wheat chromosome 2B.Here,we propose a strategy to construct the recombinant wheat chromosome 2B with multiple resistances to stripe rust by making crosses between wheat lines or cultivars carrying Yr genes and using marker-assisted selection,based on the reported information about resistance spectrum,chromosomal location,and linked markers of the genes.Pyramiding the resistance genes on 2B would afford a valuable strategy to control the disease by cultivating varieties with durable resistance.The possibility,efficiency,and prospect of the suggested strategy are reviewed in the paper. | Peigao Luo Xueyun Hu Huaiyu Zhang Zhenglong Ren | 2009 | Progress in Natural Science:Materials International2009，19，1： | 3 |
| 5 | eDNA, genomic sequence and overexpression of crystallin alpha- B Gene (CRYAB) of the Giant Panda显示文摘 | Hou Yiling Hou Wanru Ren Zhenglong | 2008 | Int J Biol Sci2008，4，6： | 1 |
| 6 | Investigation on the electronically excited state properties of multiwalled carbon nanotube (MDDA) in solution显示文摘Sub-microsecond time-resolved absorption spectroscopy has been used to study the electronically excited state behavior of soluble multiwalled carbon nanotube (MWNTsCON((CH2)9- CH3)2, denoted as MDDA) in chloroform, toluene and cyclohexane. Following pulsed photo-excitation of MDDA at 355 nm, three major spectral components are clearly identified with the help of global analysis carried out over 7 representative kinetics curves from 450 to 700 nm. The solvent depend-ence of decay associated difference spectra (DADS) and the corresponding lifetimes help to assign these transient species to singlet state (S1), triplet state (T1) and charge-separated state (CS), re-spectively. Preliminary discussion had been made to explore the involved photophysical and electron transfer processes. | FENG Juan YANG Zujun REN Zhenglong | 2006 | Science China Chemistry2006，49，2： | 1 |
| 7 | Preparation, characterization and release of methyl viologen from a novel nanoparticle delivery system with double shells of silica and PLGA显示文摘The use of nanotechnology in drug delivery is a rapidly expanding field. Biodegradable or nontoxic nanomaterials have the most promising application potentials in nanomedicine. Herein, we report a novel core-shell nanoparticle with double shell coatings (silica and poly(D,L-lactide-co-glycolide) (PLGA)) with the total shell thickness of (8.7 ± 1.3) nm. The outer shell of PLGA is biodegradable and used for controlled and sustained release, and the inner shell of silica is mesoporous for the preservation of the chemical radiation therapeutic of methyl viologen (MV), an oxidant that produces reactive oxygen species during cancer radiation therapy. The dissolution time course data and transmission electron microscopy images showed that the novel nanoparticles (Au@SiO2&PLGA) have been successfully prepared, and silica and PLGA coated well the gold (Au) template surfaces. Nano- capsules (MV@SiO2&PLGA) were obtained after the gold templates were dissolved using sodium cyanide. The sustained release property was characterized through detecting fluorescence quenching time course of fluorescent isothiocyanate after mixing with MV@SiO2&PLGA nanocapsules that encapsulate MV molecules. The sustained release of MV molecules could be extended to approximately four weeks. This novel delivery system has high potential in future application for the delivery of therapeutic drugs, particularly for the treatment of cancer by radiation therapy. | YANG Hong MIYOSHI Hirokazu LOU ChangChun REN ZhengLong LIU YiYao | 2010 | Chinese Science Bulletin2010，55，3： | 1 |
| 8 | Plant regeneration from leaves of sweet and sour cherry cultivars显示文摘 | Tang Haoru Ren Zhenglong Reustle G | 2002 | Scientia Horticulturae2002，93，： | 1 |
| 9 | RETRACTED ARTICLE: Analysis of DNA methylation polymorphism in a set of stable chromosome translocation lines显示文摘 | Shuangrong Li Yong Zhang Kejun Deng Jinhua Peng Xuelian Zeng Huaiqiong Zhang Zhenglong Ren | 2013 | Molecular Breeding2013，，1： | 1 |
| 10 | PLGA nanoparticle encapsulated paclitaxol for sustained release and its anticancer effect for HeLa cells in vitro显示文摘Poly (D,L-lactide-co-glycolide) (PLGA) is a biodegradable and biocompatible polymer material for drug deliver system. The aim of this study is to synthesize drug-loaded | Hong Yang, Kai Li, Zhonghua Liu, Zhenglong Ren, Yiyao Liu , Department of Biophysics, School of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu, Sichuan 610054, P.R.China | 2009 | 生物物理学报2009，25，S1： | 0 |
| 11 | Adjustment time of the first baroclinic Rossby wave in the global oceans显示文摘According to the freely linear Rossby wave theory, global 1°×1° climatology of Rossby deformation radius and phase speed are studied under the flat bottom fluid approximation. Geographical variations in the contours of the phase speed are very similar to those of the Rossby radius of formation, which are mainly affected by stratification, rotation, water depth, topography, etc. The basin-crossing time of the first baroclinic Rossby wave is obtained and analyzed in the global ocean basins. The results are useful to learn the importance of Rossby wave and the adiabatic adjustment of large-scale ocean circulation. | FAN Haimei LI Bingrui XU Ren ZHANG Zhenglong LI Yihong | 2006 | Acta Oceanologica Sinica2006，25，6： | 0 |
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