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| 1 | JWA,a novel microtubule-associated protein,regulates homeostasis of intracellular amino acids in PC12 cells显示文摘Our previous studies demonstrated that JWA, a novel retinoic acids responsive and cytoskeleton related gene, is associated with cell differentiation and apoptosis. In the present study, to elucidate if the JWA is a novel kind of microtubule-associated proteins (MAPs) and functionally link to microtubule, we first successfully identified JWA from the physically purified MAPs complex of rat brain tissues. The results of co-immunoprecipitation, gene trans-fection and immunofluorescence microscopy assays from HBE and NIH3T3 cells provide strong evidence for a linkage between JWA and b-tubulin. In general, JWA is stably binding to b-tubulin whenever microtubule is polymerized or not, and it may be critical to the mitosis process. In addi-tion, by use of the antisense oligonucleotides technique, we also showed that JWA is a negative modulator on intracellu-lar amino acids in PC12 cells. Further analysis indicated that JWA selectively regulates both taurine, an inhibitory amino acid, and glutamate, an excitatory amino acid. In conclusion, JWA is not only structurally associated, but also a novel functional MAP. | LIAiqun LIAiping MAOWenqe CHENHairong HUANGShu QIHong YEJian ZHANGZhengdong WANGXinru SUNFei ZOUChangping ZHOUJianwei | 2003 | Chinese Science Bulletin2003，48，17： | 13 |
| 2 | JWA protein binds to α-tubulin in PC12 cells显示文摘Our previous study elucidated that JWA protein was a newly identified microtubule-associated protein (MAP), which combined to and co-localized with α-tubulin. In the present study, we designed a series of experiments to explore if any interactions between JWA protein and α-tubulin existed and how JWA protein would functionally link to α-tubulin, especially in cell mitosis. Results of coimmnnoprecipitation, gene transfection and immnnoflnores-cence microscopy from PC12 and HEK293 cells provided strong evidence for a linkage between JWA protein and α-tubulin. Our data showed that JWA protein bound to α-tubulin stably no matter whether α-tubulin was polymerized or not. In addition, by using antisense oligonncleotides, cell cycle blocking agents and hypothermia disposal techniques, we also found the interaction between JWA protein and α-tubulin. The further analysis using flow cytometry and confocal microscopy showed that both proteins co-existed in PC12 cells and were independent on the cell cycle. In conclusion, JWA protein is a newly identified microtnbnle-associated protein, binds to α-tubulin, and probably plays an important role in regulation of microtnbnlar stability. | CHENHairong LIAiqun LIAiping ZHOUJianwei | 2004 | Chinese Science Bulletin2004，49，5： | 6 |
| 3 | ResearchonBuildingMethodsofPartsandComponentsLibrary显示文摘 | LiuXuemei LiAiping Zhu Wenbo | 2006 | JournalofEngineeringGraphics2006，27，1： | 1 |
| 4 | Facing the TSPsolution of the hy-brid ant colony algorithm 显示文摘 | ZhangHong LiAiPing LiuXueMei | 2009 | Computer engineering2009，35，8： | 1 |
| 5 | Absorption-sublimation Model for a Freeze Dryer显示文摘 | Litchfield R T Liaips A I | 1979 | Chemical Engineering Science1979，34，9： | 1 |
| 6 | Facing the TSP solution of the hybrid ant colony algorithm显示文摘 | ZhangHong LiAiPing LiuXueMei | | 0，，08： | 1 |
| 7 | Cartotid setenosis: factors affecting symomatology 显示文摘 | Liaips CD Kakisis JD Kostakis AG | 2001 | Stroke2001，32，12： | 1 |
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