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| 1 | Engineered targeting tLyp-1 exosomes as gene therapy vectors for efficient delivery of siRNA into lung cancer cells显示文摘Natural exosomes can express specific proteins and carbohydratemolecules on the surface and hence have demonstrated the great potentials for gene therapy of cancer.However,the use of natural exosomes is restricted by their low transfection efficiency.Here,we report a novel targeting tLyp-1 exosome by gene recombinant engineering for delivery of siRNA to cancer and cancer stem cells.To reach such a purpose,the engineered tLyp-1-lamp2b plasmids were constructed and amplified in Escherichia coli.The tLyp-1-lamp2b plasmids were further used to transfect HEK293T tool cells and the targeting tLyp-1 exosomes were isolated from secretion of the transfected HEK293T cells.Afterwards,the artificially synthesized siRNA was encapsulated into targeting tLyp-1 exosomes by electroporation technology.Finally,the targeting siRNA tLyp-1 exosomes were used to transfect cancer or cancer stem cells.Results showed that the engineered targeting tLyp-1 exosomes had a nanosized structure(approximately 100 nm)and high transfection efficiency into lung cancer and cancer stem cells.The function verifications demonstrated that the targeting siRNA tLyp-1 exosomes were able to knock-down the target gene of cancer cells and to reduce the stemness of cancer stem cells.In conclusion,the targeting tLyp-1 exosomes are successfully engineered,and can be used for gene therapy with a high transfection efficiency.Therefore,the engineered targeting tLyp-1 exosomes offer a promising gene delivery platform for future cancer therapy. | Jing Bai Jialun Duan Rui Liu Yafei Du Qian Luo Yinuo Cui Zhanbo Su Jiarui Xu Ying Xie Wanliang Lu | 2020 | Asian Journal of Pharmaceutical Sciences2020，15，4： | 5 |
| 2 | CO removal by two-stage methanation for polymer electrolyte fuel cell显示文摘为了移开公司完成,降低公司内容在在为聚合物电解质的 reformer 的 CO 移动步的 10 ppm 下面燃料房间(PEFC ) 合作产生系统,在各种各样的条件下面的公司优先的 methanation 在这篇论文被学习。结果证明与催化剂的一种单个类型,到达两公司移动深度和公司 2 变换比率是困难的低于 5% 。因此,使用二种催化剂的一个二阶段的 methanation 过程在这研究被建议,也就是说有相对低的活动的一个种催化剂和为在更高的温度的第一个阶段的高选择,并且有为在更低的温度的第二个阶段的相对高的活动和高选择的另一种催化剂。试验性的结果证明在第一个阶段公司,内容从 1% 被减少到低于 0.1% 在 250 300 ° C,并且在第二个阶段到在在 150 185 ° C 的 10 ppm 下面。公司 2 变换被作为不到 5% 。同时,在公司移动深度上入口公司内容和 GHSV 影响也在这篇论文被讨论。 | Zhiyuan Li Wanliang Mi Juan Gong Zhenlong Lu Lihao Xu Qingquan Su | 2008 | Journal of Natural Gas Chemistry2008，17，4： | 5 |
| 3 | Separation of injectable salidroside by column chromatography of macroporous resins for treating myocardial ischemia显示文摘The objective of the present study is to develop a method for large-scale separating and purifying salidroside from rhodiola kirilowii roots and for preparing injectable medicinal ingredient.Crude extract of salidroside was prepared by water-ethanol system,and purified by column chromatography of macroporous resins.Static adsorption and desorption studies were performed on six kinds of macroporous resins,and SP825 resin was chosen,followed by optimizing process parameters.The optimum sample volume,feed concentration,ratio of diameter to height,and feeding flow rate were 1.5 bed volumes(BV),15 mg/mL,1:10 and 1 BV/h,respectively.Dynamic desorption was performed consecutively with 8 BV of distilled water,3 BV of 5% ethanol and 8 BV of 10% ethanol at a flow rate of 2 BV/h.After three cycles in separating 3.5 tons of rhodiola kirilowii roots,salidroside purity was increased from 3.4% in the crude extract to 93.6% in purified salidroside product.This study provides a novel method to separate salidroside for injectable use. | JU RuiJun HUANG RenJie ZHOU Jia LI RuoJing ZHOU Peng ZHANG ZaoHua XIANG FeiJun XU DongJin LIU WeiXiang MA XingTian ZHANG Qiang LU WanLiang | 2012 | Science China Chemistry2012，55，7： | 2 |
| 4 | Pharmacokineties, toxicity of nasal cilia and immunomodulating effects in Sprague-Dawley rats following intranasal delivery of thymopentin with or without absorption enhancers显示文摘 | Wang Jing Lu Wanliang Liang Gongwen | 2006 | Peptides2006，27，4： | 1 |
| 5 | Con- trolled delivery of recombinant hirudin based on thermo- sensitive Pluronic F127 hydrogel for subcutaneous ad- ministration: in vitro and in vivo characterization 显示文摘 | LIU Yu LU Wanliang WANG Jiancheng | 2007 | Journal of Controlled Release2007，117，3： | 1 |
| 6 | Targeted core-shell nanoparticles for precise CTCF gene insert in treatment of metastatic breast cancer显示文摘Clustered regularly interspaced short palindromic repeats(CRISPR)technology emerges a remarkable potential for cure of refractory cancer like metastatic breast cancer.However,how to efficiently deliver the CRISPR system with non-viral carrier remains a major issue to be solved.Here,we report a kind of targeted core-shell nanoparticles(NPs)carrying dual plasmids(pHR-pCas9)for precise CCCTC-binding factor(CTCF)gene insert to circumvent metastatic breast cancer.The targeted core-shell NPs carrying pHR-pCas9 can accomplishγGTP-mediated cellular uptake and endosomal escape,facilitate the precise insert and stable expression of CTCF gene,inhibit the migration,metastasis,and colonization of metastatic breast cancer cells.Besides,the finding further reveals that the inhibitory mechanism of metastasis could be associated with up-regulating CTCF protein,followed by down-regulating stomatin(STOM)protein.The study offers a universal nanostrategy enabling the robust non-viral delivery of gene-editing system for treatment of severe illness. | Jialun Duan Chunjie Bao Ying Xie Haitao Guo Yixuan Liu Jianwei Li Rui Liu Peishan Li Jing Bai Yan Yan Limin Mu Xueqi Li Guiling Wang Wanliang Lu | 2022 | Bioactive Materials2022，7，5： | 1 |
| 7 | Dual targeting dauno rubicin liposomes improve the therapeutic efficacy of brain glio ma in animals 显示文摘 | Ying Xue Wen He Lu Wanliang | 2009 | J Control Release2009，141，2： | 1 |
| 8 | Switchable nanoparticles complexing cisplatin for circumventing glutathione depletion in breast cancer chemotherapy显示文摘Cisplatin is broad-spectrum chemotherapeutic agent that has been widely used for the treatment of a variety of malignant tumors including breast cancer.However,the cisplatin chemoresistance,which derives from the inactivation by glutathione(GSH)depletion,remains a scientific issue to solve.Here,we report a novel type of smart disulfide switchable nanoparticles complexing cisplatin(switch NPs-cisplatin)that is rationally designed,and engineered by synthesizing a hyaluronic acid disulfide bonded polyaspartic acid(HA-ss-Pasp)and complexing cisplatin.The results showed that the switch NPs-cisplatin had a nanoscale of particle size(150 nm),higher drug encapsulation efficiency(>90%),and suitable drug release profile.They demonstrated evident pH responsiveness and GSH responsiveness,and targeting effect in the resistant breast cancer cells.Furthermore,they were able to block the cisplatin depletion by GSH in the resistant cancer cells,thereby circumventing the chemoresistance.Consequently,switch NPs-cisplatin displayed a remarkable killing effect in the resistant breast cancer cells in vitro,and in the resistant breast cancer-bearing mice.In conclusion,switch NPs-cisplatin could be used as a smart formulation of cisplatin for overcoming the chemoresistance of breast cancer.The present study also offers a universal drug delivery carrier platform for highly efficient but low systemic toxic chemotherapy. | Ming Chen Ying Xie Qian Luo Jiarui Xu Yuxin Ren Rui Liu Huihui Zhao Yuling Chen Hexuan Feng Yafei Du Jianwei Li Guiling Wang Wanliang Lu | 2023 | Chinese Chemical Letters2023，34，5： | 0 |
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