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| 1 | Preparation of rabbit anti-rat LRRN3 polyclonal antibody and study of its expression显示文摘BACKGROUND:Studies have shown that the LRRN3,a member of the Neuron Leucine-Rich Repeat superfamily,could be related to neural development,differentiation,information transmission,and other functions,but most studies have focused on nucleic acid levels and few have reported on LRRN3 protein levels.OBJECTIVE:To prepare rabbit anti-rat LRRN3 polyclonal antibody and to observe protein tissue expression profiles.DESIGN,TIME AND SETTING:In vitro,molecular,biological experiments were performed from October 2007 to April 2009 in Laboratory of Neurobiology at Xiangya School of Medicine,Central South University.MATERIALS:Immunization antigen,namely rat Mal-LRRN3C-His recombinant protein,was provided by the Laboratory of Neurobiology at Xiangya School of Medicine,Central South University.METHODS:Rat Mal-LRRN3C-His recombinant protein was used to immunize male,New Zealand rabbits,and rabbit anti-rat LRRN3 polyclonal antibody was prepared.MAIN OUTCOME MEASURES:Antibody purification was conducted using Protein A affinity chromatography,and the LRRN3 anti-serum titer was identified using enzyme-linked immunosorbent assay.Immunohistochemical techniques and Western blot preliminary tests were used to determine LRRN3 protein expression profiles in adult rats.RESULTS:A highly purified rabbit anti-rat LRRN3 polyclonal antibody was obtained.Western Blot results from rat brain total protein revealed a band at 79 kD,which was consistent with the size of LRRN3.Immunohistochemistry results showed that protein was mainly expressed in the central nervous system,and no significant positive signals were observed in other tissues.Positive cells included neurons of cerebral cortex and hippocampal dentate gyrus granule cell layer,and cerebellar Purkinje cells.There was no positive expression in glial cells.CONCLUSION:Rabbit anti-rat LRRN3 polyclonal antibody was successfully prepared at a high purity from the prokaryotic-expressed Mal-LRRN3C-His recombinant protein,which served as an antigen.Rat LRRN3 protein was primarily expressed in cerebral cortex neurons,hippocampal dentate gyrus granule cell layer neurons,and cerebellar Purkinje cells. | Jing Yang Yanjin Wang Yan Cai Zhiying Yang Ganqiu Wu Jianguo Wen Xuegang Luo He Huang | 2010 | Neural Regeneration Research2010，5，13： | 3 |
| 2 | Myocardin-related transcription factor A is up-regulated by 17β-estradiol and promotes migration of MCF-7 breast cancer cells via transactivation of MYL9 and CYR61显示文摘证据的许多线建议了雌激素不仅在乳癌的开始和增长,而且在癌症转移起重要作用。然而,后者事件的机械学的基础糟糕被理解。另外,最近的研究建议了那 myocardin 相关的抄写因素 A (MRTF -- 一) 可能与癌症有关转移。然而报告是矛盾的它的角色肯定尚待糊涂。在现在的学习,我们证明过多的 17-estradiol 能支持 MCF-7 乳癌房间和起来调整的迁居 MRTF 的表示 -- 一,肌浆球蛋白规章的轻链 9 (MYL9 ) ,并且充满半胱氨酸的 angiogenic inducer 61 (CYR61 ) 。MRTF 的 Overexpression -- A 显著地在 MYL9 和 CYR61 基因上通过它的 transactivation 效果支持了 MCF-7 房间的移植,当时 RNA 调停干扰 MRTF 击倒 -- A 强烈禁止了目标基因的抄写和表示并且减少了 MCF-7 房间的移植能力。这些结果提供了支持 MRTF 的支持转移的功能的新奇证据 -- 一,并且暗示了那 MRTF -- A 可能是一个开关让雌激素小径在乳癌把它的支持增长的角色变成刺激迁居的角色。 | Chunling Zhang | 2013 | Acta Biochimica et Biophysica Sinica2013，45，11： | 3 |
| 3 | Lithium chloride ameliorates learning and memory ability and inhibits glycogen synthase kinase-3 beta activity in a mouse model of fragile X syndrome显示文摘In the present study,Fmr1 knockout mice (KO mice) were used as the model for fragile X syndrome.The results of step-through and step-down tests demonstrated that Fmr1 KO mice had shorter latencies and more error counts,indicating a learning and memory disorder.After treatment with 30,60,90,120,or 200 mg/kg lithium chloride,the learning and memory abilities of the Fmr1 KO mice were significantly ameliorated,in particular,the 200 mg/kg lithium chloride treatment had the most significant effect.Western blot analysis showed that lithium chloride significantly enhanced the expression of phosphorylated glycogen synthase kinase 3 beta,an inactive form of glycogen synthase kinase 3 beta,in the cerebral cortex and hippocampus of the Fmr1 KO mice.These results indicated that lithium chloride improved learning and memory in the Fmr1 KO mice,possibly by inhibiting glycogen synthase kinase 3 beta activity. | Shengqiang Chen Xuegang Luo Quan Yang Weiwen Sun Kaiyi Cao Xi Chen Yueling Huang Lijun Dai Yonghong Yi | 2011 | Neural Regeneration Research2011，6，31： | 3 |
| 4 | Sorption of Pb(II) from aqueous solution by konjac glucomannan beads显示文摘Konjac glucomannan beads have been investigated as metal biosorbent for Pb(II) from aqueous solu-tions. The effect of contact time, solution pH, initial metal concentration, and desorption were studied in batch experiments at 20℃±2℃. Maximum mental sorption was found to occur at initial pH 4.0―5.5. Kinetic studies revealed that the initial uptake was rapid and equilibrium was established in 3 h and that the data followed the prseudo-second order reaction. The equilibrium sorption data at initial pH 4.0 were described by the Langmuir and Freundlich isotherm models; however, Langmuir isotherm model has been found to provide the best correlation. The highest value of Langmuir maximum uptake (qmax) was found to be 105.71 mg·g-1. Similar Freundlich empirical constant (KF) was obtained to be 1.98 for lead. Adsorption-complexation may be involved in the sorption process of lead. Desorption experi- ments showed evidence that after two contacts neither HCl nor EDTA solutions were able to desorb lead from the konjac glucomannan beads, but the desorbtion efficacy of HCl solution was higher than EDTA solution. The results obtained show that konjac glucomannan beads may be used for the treat-ment of wastewater contaminated with lead. | LONG XiaoYan LUO XueGang WANG Yang LI Zheng | 2009 | Science China(Technological Sciences)2009，52，1： | 3 |
| 5 | Histone acetyltransferase p300 promotes MKLl-mediated transactivation of catechol-O-methyltransferase gene显示文摘以前的研究表明了 histone acetyltransferase p300 被招募到某些心脏、光滑的肌肉的倡导者特定的基因到提高 myocardin 的 transactivation 活动,它是在心血管的区别和开发的一个主人管理者。这里,我们发现编码 catechol-O-methyltransferase (COMT ) 的基因,催化雌激素的变换的重要的新陈代谢的酶,也是 myocardin 相关的抄写因素(MRTF ) 的目标基因。Megakaryoblastic 白血病 1 (MKL1,也命名的 MRTF -- 一) 并且 p300 能 synergistically 扩充 COMT 基因的表示,增加雌激素的新陈代谢的率,并且因此减少雌激素刺激的 MCF-7 乳癌房间的增长。 | Zhipeng Liu Xuegang Luo Lei Liu Wenwen Zhao Shu Guo Yu Guo Nan Wang Hongpeng He XinghuaLiao Wenjian Ma Hao Zhou Tongcun Zhang | 2013 | Acta Biochimica et Biophysica Sinica2013，45，12： | 1 |
| 6 | Expression of endothelial nitric oxide synthase in the vascular wall during arteriogenesis 显示文摘 | Wei - jun Cai 1 Elisabeth Kocsis 2 Xuegang Luo | 2004 | Molecular and Cellular Biochemistry2004，264，： | 1 |
| 7 | Effect of degree of acetylation on thermoplastic and melt rheological properties of acetylated konjac glucomannan显示文摘 | Xiaoyan Lin Qiang Wu Xuegang Luo Feng Liu Xiaoqing Luo Pan He | 2010 | Carbohydrate Polymers2010，，1： | 1 |
| 8 | Preparation and characterization of hollow glass microsphere reinforced poly (butylenes succinate) composites 显示文摘 | Jiwei Li Xuegang Luo | 2013 | Materials and Design2013，，46： | 1 |
| 9 | Adsorption of tannin from aqueous solution by deacetylated konjac glucomannan显示文摘 | Feng Liu Xuegang Luo Xiaoyan Lin | 2010 | Journal of Hazardous Materials2010，，1： | 1 |
| 10 | Mechanical and thermalperformance of distillers grains filled poly (butylene succinate)composites 显示文摘 | Luo Xuegang Li Jiwei Feng Juan | 2014 | Materials & Design2014，57，19： | 1 |
| 11 | An effective method of DNA extraction for bioleaching bacteria from acid mine drainage显示文摘 | Leping Zeng Jufang Huang Yanfei Zhang Guanzhou Qiu Jianbin Tong Dan Chen Jin Zhou Xuegang Luo | 2008 | Applied Microbiology and Biotechnology2008，，5： | 1 |
| 12 | A sorbent of carboxymethyl cellulose loaded with zirconium for the removal of fluoride from aqueous solution显示文摘 | Jing Wang Xiaoyan Lin Xuegang Luo Yufeng Long | 2014 | Chemical Engineering Journal2014，，： | 1 |
| 13 | Fixed-bed column study for Cu2+ removal from solution using expan- ding rice husk显示文摘 | Luo Xuegang Deng Zaifang Lin Xiaoyan | 2011 | J Hazard Mater2011，187，1： | 1 |
| 14 | Comparative study on the blends of PBS / thermoplastic starch prepared from waxy and normal corn starches 显示文摘 | Li Jiwei Luo Xuegang Lin Xiaoyan | 2013 | Starch2013，65，： | 1 |
| 15 | A sorbent of carboxymethyl cellulose loaded with zirconium for the removal of fluoride from aqueous solution显示文摘 | Wang Jing Lin Xiaoyan Luo Xuegang | 2014 | Chemical Engineering Journal2014，，252： | 1 |
| 16 | Evaluation of distillers grains as fillers for low density polyethylene: Mechanical, rheological and thermal characterization 显示文摘 | Xuegang Luo Jiwei Li Juan Feng Songzhi Xie Xiaoyan Lin | 2013 | Composites Science and Technology2013，，89： | 1 |
| 17 | A modi- fied lignin adsorbent for the removal of 2, 4, 6-trinitrotoluene 显示文摘 | Zhang Jianping Lin Xiaoyan Luo Xuegang | 2011 | ChemEngJ2011，168，3： | 1 |
| 18 | Removal of aniline using lignin grafted acrylic acid from aqueous solution显示文摘 | Lin Xiaoyan Zhang Jianping Luo Xuegang | 2011 | Chemical Engineering Journal2011，172，23： | 1 |
| 19 | Histone methyltransferase SMYD3 promotes MRTF-Amediated transactivation of MYL9 and migration of MCF-7 breast cancer cells显示文摘 | Luo Xuegang Zhang Chunling Zhao Wenwen | 2014 | Cancer Letters2014，344，1： | 1 |
| 20 | Removal of Aniline using lignin grafted acrylic acid from aqueous solution显示文摘 | Lin Xiaoyan Zhang Jianping Luo Xuegang | 2011 | Chem Eng J2011，172，2： | 1 |
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