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| 1 | Characterization of microRNAs in serum: a novel class of biomarkers for diagnosis of cancer and other diseases显示文摘在各种各样的纸巾的 microRNAs (miRNAs ) 的 Dysregulated 表示与许多疾病被联系了,包括癌症。这里,我们证明 miRNAs 在象老鼠,老鼠,牛的胎儿,小牛,和马那样的人和另外的动物的浆液和血浆是在场的。在浆液的 miRNAs 的层次在一样的种类的个人之中稳定、可再现、一致。采用 Solexa,我们定序健康中国题目的所有浆液 miRNAs 并且分别地在男、女的题目发现超过 100 和 91 浆液 miRNAs。我们也为肺癌症, colorectal 癌症,和糖尿病识别了浆液 miRNAs 的特定的表示模式,提供证据那浆液 miRNAs 为各种各样的疾病包含指纹。癌症特定的浆液 miRNAs 由 Solexa 获得了的二个非小的房间肺进一步在 75 个健康施主和 152 个癌症病人的独立审判被验证,用量的反向的抄写聚合酶链反应试金。通过这些分析,我们断定浆液 miRNAs 能为各种各样的癌症和另外的疾病的察觉用作潜在的 biomarkers。 | Xi Chen Yi Ba Lijia Ma Xing Cai Yuan Yin Kehui Wang Jig ang Guo Yujing Zhang Jiangning Chen Xing Guo Qibin Li Xiaoying Li Wenjing Wang Yan Zhang Jin Wang Xueyuan Jiang Yang Xiang Chen Xu Pingping Zheng Juanbin Zhang Ruiqiang Li Hongjie Zhang Xiaobin Shang Ting Gong Guang Ning Jun Wang Ke Zen Junfeng Zhang Chen-Yu Zhang | 2008 | Cell Research2008，18，10： | 975 |
| 2 | A rapid advice guideline for the diagnosis and treatment of 2019 novel coronavirus(2019-nCoV) infected pneumonia(standard version)显示文摘In December 2019, a new type viral pneumonia cases occurred in Wuhan, Hubei Province;and then named '2019 novel coronavirus(2019-nCoV)' by the World Health Organization(WHO) on 12 January 2020. For it is a never been experienced respiratory disease before and with infection ability widely and quickly, it attracted the world’s attention but without treatment and control manual. For the request from frontline clinicians and public health professionals of 2019-nCoV infected pneumonia management, an evidence-based guideline urgently needs to be developed. Therefore, we drafted this guideline according to the rapid advice guidelines methodology and general rules of WHO guideline development;we also added the first-hand management data of Zhongnan Hospital of Wuhan University. This guideline includes the guideline methodology, epidemiological characteristics, disease screening and population prevention, diagnosis, treatment and control(including traditional Chinese Medicine), nosocomial infection prevention and control, and disease nursing of the 2019-nCoV. Moreover, we also provide a whole process of a successful treatment case of the severe 2019-nCoV infected pneumonia and experience and lessons of hospital rescue for 2019-nCoV infections. This rapid advice guideline is suitable for the first frontline doctors and nurses, managers of hospitals and healthcare sections, community residents, public health persons, relevant researchers, and all person who are interested in the 2019-nCoV. | Ying-Hui Jin Lin Cai Zhen-Shun Cheng Hong Cheng Tong Deng Yi-Pin Fan Cheng Fang Di Huang Lu-Qi Huang Qiao Huang Yong Han Bo Hu Fen Hu Bing-Hui Li Yi-Rong Li Ke Liang Li-Kai Lin Li-Sha Luo Jing Ma Lin-Lu Ma Zhi-Yong Peng Yun-Bao Pan Zhen-Yu Pan Xue-Qun Ren Hui-Min Sun Ying Wang Yun-Yun Wang Hong Weng Chao-Jie Wei Dong-Fang Wu Jian Xia Yong Xiong Hai-Bo Xu Xiao-Mei Yao Yu-Feng Yuan Tai-Sheng Ye Xiao-Chun Zhang Ying-Wen Zhang Yin-Gao Zhang Hua-Min Zhang Yan Zhao Ming-Juan Zhao Hao Zi Xian-Tao Zeng Yong-Yan Wang Xing-Huan Wang 无 | 2020 | Military Medical Research2020，7，1： | 161 |
| 3 | The Chinese Society of Clinical Oncology(CSCO):Clinical guidelines for the diagnosis and treatment of gastric cancer,2021显示文摘There exist differences in the epidemiological characteristics,clinicopathological features,tumor biological characteristics,treatment patterns,and drug selections between gastric cancer patients from the Eastern and Western countries.The Chinese Society of Clinical Oncology(CSCO)has organized a panel of senior experts specializing in all sub-specialties of gastric cancer to compile a clinical guideline for the diagnosis and treatment of gastric cancer since 2016 and renews it annually.Taking into account regional differences,giving full consideration to the accessibility of diagnosis and treatment resources,these experts have conducted expert consensus judgment on relevant evidence and made various grades of recommendations for the clinical diagnosis and treatment of gastric cancer to reflect the value of cancer treatment and meeting health economic indexes in China.The 2021 CSCO Clinical Practice Guidelines for Gastric Cancer covers the diagnosis,treatment,follow-up,and screening of gastric cancer.Based on the 2020 version of the CSCO Chinese Gastric Cancer guidelines,this updated guideline integrates the results ofmajor clinical studies from China and overseas for the past year,focused on the inclusion of research data from the Chinese population for more personalized and clinically relevant recommendations.For the comprehensive treatment of non-metastatic gastric cancer,attentions were paid to neoadjuvant treatment.The value of perioperative chemotherapy is gradually becoming clearer and its recommendation level has been updated.For the comprehensive treatment of metastatic gastric cancer,recommendations for immunotherapy were included,and immune checkpoint inhibitors fromthird-line to the first-line of treatment for different patient groups with detailed notes are provided. | Feng-Hua Wang Xiao-Tian Zhang Yuan-Fang Li Lei Tang Xiu-Juan Qu Jie-Er Ying Jun Zhang Ling-Yu Sun Rong-Bo Lin Hong Qiu Chang Wang Miao-Zhen Qiu Mu-Yan Cai QiWu Hao Liu Wen-Long Guan Ai-Ping Zhou Yu-Jing Zhang Tian-Shu Liu Feng Bi Xiang-Lin Yuan Sheng-Xiang Rao Yan Xin Wei-Qi Sheng Hui-Mian Xu Guo-Xin Li Jia-Fu Ji Zhi-Wei Zhou Han Liang Yan-Qiao Zhang Jing Jin Lin Shen Jin Li Rui-Hua Xu | 2021 | Cancer Communications2021，41，8： | 97 |
| 4 | Relocation of the mainshock and aftershock sequences of M_S7.0 Sichuan Lushan earthquake显示文摘The mainshock of April 20,2013 Sichuan Lushan MS7.0 earthquake was relocated using a 3-D velocity model.Double difference algorithm was applied to relocate aftershock sequences of Lushan earthquake.The locations of 2405 aftershocks were determined.The location errors in E-W,N-S and U-D direction were 0.30,0.29 and 0.59 km on average,respectively.The location of the mainshock is 102.983°E,30.291°N and the focal depth is 17.6 km.The relocation results show that the aftershocks spread approximately 35 km in length and 16 km in width.The dominant distribution of the focal depth ranges from 10 to 20 km.A few earthquakes occurred in the shallow crust.Focal depth profiles show fault planes dip to the northwest,manifested itself as a listric thrust fault.The dip angle is steep in the shallow crust and gentle in the deep crust.Although the epicenters of aftershocks distributed mainly along both sides of the Shuangshi-Dachuan fault,the seismogenic fault may be a blind thrust fault on the eastern side of the Shuangshi-Dachuan fault.Earthquake relocation results reveal that there is a southeastward tilt aftershock belt intersecting with the seismogenic fault with y-shape.We speculate it is a back thrust fault that often appears in a thrust fault system.Lushan earthquake triggered the seismic activity of the back thrust fault. | FANG LiHua WU JianPing WANG WeiLai Lü ZuoYong WANG ChangZai YANG Ting CAI Yan | 2013 | Chinese Science Bulletin2013，58，28： | 74 |
| 5 | Transmission Dynamics of an Outbreak of the COVID-19 Delta Variant B.1.617.2——Guangdong Province,China,May-June 2021显示文摘On May 21,2021,a local case of coronavirus disease 2019(COVID-19)was confirmed in a 75-year-old woman(experienced onset of symptoms on May 18)in Liwan District,Guangzhou City,Guangdong Province,China.The number of infections has increased in the following 10 days and led to 5 generations of transmission. | Meng Zhang Jianpeng Xiao Aiping Deng Yingtao Zhang Yali Zhuang Ting Hu Jiansen Li Hongwei Tu Bosheng Li Yan Zhou Jun Yuan Lei Luo Zimian Liang Youzhi Huang Guoqiang Ye Mingwei Cai Gongli Li Bo Yang Bin Xu Ximing Huang Yazun Cui Dongsheng Ren Yanping Zhang Min Kang Yan Li | 2021 | China CDC weekly2021，3，27： | 51 |
| 6 | New insights of T cells in the pathogenesis of psoriasis显示文摘干癣是 hyperproliferative keratinocytes 和 T 房间,树枝状的房间,巨噬细胞和 neutrophils 的渗入描绘的最普通的调停免疫者的长期的、煽动性的皮肤病之一。尽管干癣的致病充分没被理解,有宽大的证据,建议在皮肤的有免疫力的房间的 dysregulation,特别地 T 房间,在干癣开发起一个关键作用。在这评论,我们主要集中于病原的 T 房间并且讨论这些 T 房间怎么被激活并且在疾病致病包含了。最新识别的 ‘ professional’IL-17-producing 真皮的 γ δ在干癣的 T 房间和他们的潜在的角色将也被包括。最后,我们将简短为干癣处理在 T 房间和它的相关指向 cytokine 的治疗上总结最近的进步。 | Yihua Cai Chris Fleming Jun Yan | 2012 | Cellular & Molecular Immunology2012，9，4： | 49 |
| 7 | Effect of subsoil tillage depth on nutrient accumulation, root distribution, and grain yield in spring maize显示文摘A four-year field experiment was conducted to investigate the effect of subsoiling depth on root morphology, nitrogen(N), phosphorus(P), and potassium(K) uptake, and grain yield of spring maize. The results indicated that subsoil tillage promoted root development,increased nutrient accumulation, and increased yield. Compared with conventional soil management(CK), root length, root surface area, and root dry weight at 0–80 cm soil depth under subsoil tillage to 30 cm(T1) and subsoil tillage to 50 cm(T2) were significantly increased, especially the proportions of roots in deeper soil. Root length, surface area, and dry weight differed significantly among three treatments in the order of T2 > T1 > CK at the12-leaf and early filling stages. The range of variation of root diameter in different soil layers in T2 treatment was the smallest, suggesting that roots were more likely to grow downwards with deeper subsoil tillage in soil. The accumulation of N, P, and K in subsoil tillage treatment was significantly increased, but the proportions of kernel and straw were different. In a comparison of T1 with T2, the grain accumulated more N and P, while K accumulation in kernel and straw varied in different years. Grain yield and biomass were increased by 12.8% and 14.6% on average in subsoil tillage treatments compared to conventional soil treatment. Although no significant differences between different subsoil tillage depths were observed for nutrient accumulation and grain yield, lodging resistance of plants was significantly improved in subsoil tillage to 50 cm, a characteristic that favors a high and stable yield under extreme environments. | Hongguang Cai Wei Ma Xiuzhi Zhang Jieqing Ping Xiaogong Yan Jianzhao Liu Jingchao Yuan Lichun Wang Jun Ren | 2014 | The Crop Journal2014，2，5： | 42 |
| 8 | Deep structure beneath the southwestern section of the Longmenshan fault zone and seimogenetic context of the 4.20 Lushan M_S7.0 earthquake显示文摘Magnetotelluric measurements were carried out along two profiles across the middle and southwestern sections of the Longmenshan fault zone(LMSf)from 2009 to 2011,after the 2008 Wenchuan MW7.9 earthquake.The former profile crosses the Wenchuan event epicenter and the latter one crosses 2013 Lushan MS7.0 event epicenter.The data were analyzed using advanced processing techniques,including phase tensor and two-dimensional inversion methods,in order to obtain reliable 2-D profiles of the electrical structure in the vicinity of the two earthquakes.A comparison of the two profiles indicates both similarities and differences in the deep crustal structure of the LMSf.West of the southwestern section,a crustal high conductivity layer(HCL)is present at about 10 km depth below the Songpan-Garzêblock;this is about 10 km shallower than that under the middle section of the LMSf.A high resistivity body(HRB)is observed beneath the southwestern section,extending from the near surface to the top of upper mantle.It has a smaller size than the HRB observed below the middle section.In the middle section,there is a local area of decreased resistivity within the HRB but there is absence of this area.The 2013 Lushan earthquake occurred close to the eastern boundary of HRB and the Shuangshi-Dachuan fault,of which the seismogenic context has both common and different features in comparison with the 2008 Wenchuan event.On a large scale,the 2013 Lushan earthquake is associated with the HCL and deformation in the crust including HCL of the eastern Tibetan Plateau.In order to assess seismic risk,it is important to consider both the stress state and the detailed crustal structure in different parts of the LMSf. | ZHAN Yan ZHAO GuoZe UNSWORTH Martyn WANG LiFeng CHEN XiaoBin LI Tao XIAO QiBin WANG JiJun TANG Ji CAI JunTao WANG YanZhao | 2013 | Chinese Science Bulletin2013，58，28： | 39 |
| 9 | Does Helicobacter pylori infection play a role in iron deficiency anemia? A meta-analysis显示文摘AIM:To perform a meta-analysis of observational studies and randomized controlled trials(RCTs)on the association between Helicobacter pylori(H.pylori)and iron deficiency anemia(IDA).METHODS:A defined search strategy was used to search Medline,Embase,the Cochrane Library,Clinical Trials,Cochrane Central Register of Controlled Trials,Premedline and Healthstar.Odds ratio(OR)was used to evaluate observational epidemiology studies,and weighted mean difference(WMD)was used to demonstrate the difference between control and intervention groups.RESULTS:Fifteen observational studies and 5 RCTs were identified and used for calculation.The pooled OR for observational studies was 2.22(95%CI:1.52-3.24,P<0.0001).The WMD for hemoglobin(HB) was 4.06 g/L(95%CI:-2.57-10.69,P=0.01),and the WMD for serum ferritin(SF)was 9.47μg/L(95%CI:-0.50-19.43,P<0.0001).Results were heterogeneous for all comparisons.CONCLUSION:This meta-analysis on observational studies suggests an association between H.pylori and IDA.In RCTs,eradication of H.pylori can improve HB and SF levels but not significantly. | Qu, Xin-Hua Huang, Xiao-Lu Xiong, Ping Zhu, Cui-Ying Huang, You-Liang Lu, Lun-Gen Sun, Xu Rong, Lan Zhong, Liang Sun, Da-Yu Lin, Hai Cai, Ming-Ci Chen, Zhi-Wei Hu, Bing Wu, Lian-Ming Jiang, Yi-Bin Yan, Wei-Li | 2010 | World Journal of Gastroenterology2010，16，7： | 34 |
| 10 | A Positioning System based on Communication Satellites and the Chinese Area Positioning System(CAPS)显示文摘The Chinese Area Positioning System(CAPS) is a positioning system based on satellite communication that is fundamentally different from the 3'G'(GPS,GLONASS and GALILEO) systems.The latter use special-purpose navigation satellites to broadcast navi-gation information generated on-board to users,while the CAPS transfers ground-generated navigation information to users via the communication satellite.In order to achieve accurate Positioning,Velocity and Time(PVT),the CAPS employs the following strategies to over-come the three main obstacles caused by using the communication satellite:(a) by real-time following-up frequency stabilization to achieve stable frequency;(b) by using a single carrier in the transponder with 36 MHz band-width to gain sufficient power;(c) by incorporating Decommissioned Geostationary Orbit communication satellite(DGEO),barometric pressure and Inclined Geostationary Orbit communication satellite(IGSO) to achieve the 3-D posi-tioning.Furthermore,the abundant transponders available on DGEO can be used to realize the large capacity of communication as well as the integrated navigation and communication.With the communication functions incorporated,five new functions appear in the CAPS:(1) combination of navigation and communication;(2) combination of navigation and high accu-racy orbit measurement;(3) combination of navigation message and wide/local area differen-tial processing;(4) combination of the switching of satellites,frequencies and codes;and(5) combination of the navigation message and the barometric altimetry.The CAPS is thereby labelled a PVT5C system of high accuracy.In order to validate the working principle and the performance of the CAPS,a trial system was established in the course of two years at a cost of about 20 million dollars.The trial constellation consists of two GEO satellites located at E87.5° and E110.5°,two DGEOs located at E130° and E142°,as well as barometric altimetry as a virtual satellite.Static and dynamic performance tests were completed for the Eastern,the Western,the Northern,the Southern and the Middle regions of China.The evaluation results are as follows:(1) land static test,plane accuracy range:C/A code,15~25m;P code,5~10 meters;altitude accuracy range,1~3 m;(2) land dynamic test,plane accuracy range,C/A code,15~25m;P code,8~10m;(3) velocity accuracy,C/A code,0.13~0.3ms-1,P code,0.15~0.17ms-1;(4) timing accuracy,C/A code,160ns,P code,13 ns;(5) timing compared accuracy of Two Way Satellite Time and Frequency Transfer(TWSTFT),average accuracy,0.068ns;(6) random error of the satellite ranging,10.7 mm;(7) orbit determination accuracy,better than 2 m.The above stated random error is 1σ error.At present,this system is used as a preliminary operational system and a complete system with 3 GEO,3 DGEO and 3 IGSO is being established. | Guo-Xiang Ai Hu-Li Shi Hai-Tao Wu Yi-Hua Yan Yu-Jing Bian Yong-Hui Hu Zhi-Gang Li Ji Guo Xian-De Cai | 2008 | Chinese Journal of Astronomy and Astrophysics2008，8，6： | 33 |
| 11 | Clinicopathologic significance of HER-2/neu protein expression and gene amplification in gastric carcinoma显示文摘AIM:To study the HER-2/neu protein expression and gene amplification in gastric carcinoma and their relation.METHODS:One hundred and forty-five formalin-fixed and paraffin-embedded tumor tissue samples from Chinese gastric carcinoma patients were studied with immunohistochemistry(IHC)and fluorescence in situ hybridization(FISH)methods.Clinicopathologic data about all patients were collected.RESULTS:The levels of HER-2 3+,HER-2 2+and HER21+were measurable in 6.9%,8.3%and 17.2%of the samples,respectively.No HER-2 was stained in 67.6% of the samples.FISH showed that HER-2 gene was amplified in 18 samples,10 HER-2 3+samples,5 HER-2 2+samples,and 3 HER-2 1+samples with IHC staining.HER-2 status was not correlated with the sex and age of patients,and tumor size,location or differentiation,but with the depth of invasion,TNM stage,lymph node and distant metastasis as well as histopathological classification of gastric cancer(P<0.05).CONCLUSION:All samples with IHC as HER-2 expression should be analyzed with FISH.Detection of HER-2 gene amplification can assess the malignant biological behaviors and prognosis of gastric cancer. | Shi-Yan Yan Ying Hu Jian-Gao Fan Guo-Quan Tao Yong-Ming Lu Xu Cai Bao-Hua Yu Yi-Qun Du | 2011 | World Journal of Gastroenterology2011，17，11： | 34 |
| 12 | Astragalus polysaccharides can regulate cytokine and P-glycoprotein expression in H22 tumor-bearing mice显示文摘AIM:To investigate the adjunct anticancer effect of Astragalus polysaccharides in H22 tumor-bearing mice.METHODS:To establish a solid tumor model,5.0 × 10 6 /mL H22 hepatoma cells were inoculated subcutaneously into the right armpit region of Kunming mice(6-12 wk old,18-22 g).When the tumors reached a size of 100 mm 3,the animals were treated as indicated,and the mice were randomly assigned to seven groups(n = 10 each).After ten days of treatment,blood samples were collected from mouse eyes,and serum was harvested by centrifugation.Mice were sacrificed,and the whole body,tumor,spleen and thymus were weighed immediately.The rate of tumor inhibition and organ indexes were calculated.The expression levels of serum cytokines,P-glycoprotein(P-GP) and multidrug resistance(MDR) 1 mRNA in tumor tissues were detected using enzyme-linked immunosorbent assay,Western blotting,and quantitative myeloid-derived suppressor cells reverse transcription-polymerase chain reaction,respectively.RESULTS:The tumor inhibition rates in the treatment groups of Adriamycin(ADM) + Astragalus polysaccharides(APS)(50 mg/kg),ADM + APS(100 mg/kg),and ADM + APS(200 mg/kg) were significantly higher than in the ADM group(72.88% vs 60.36%,P = 0.013;73.40% vs 60.36%,P = 0.010;77.57% vs 60.36%,P = 0.001).The spleen indexes of the above groups were also significantly higher than in the ADM group(0.65 ± 0.22 vs 0.39 ± 0.17,P = 0.023;0.62 ± 0.34 vs 0.39 ± 0.17,P = 0.022;0.67 ± 0.20 vs 0.39 ± 0.17,P = 0.012),and the thymus indexes of the ADM + APS(100 mg/kg) and ADM + APS(200 mg/kg) groups were significantly higher than in the ADM group(0.20 ± 0.06 vs 0.13 ± 0.04,P = 0.029;0.47 ± 0.12 vs 0.13 ± 0.04,P = 0.000).APS was found to exert a synergistic antitumor effect with ADM and to alleviate the decrease in the sizes of the spleen and thymus induced by AMD.The expression of interleukin-1α(IL-1α),IL-2,IL-6,and tumor necrosis factor-α(TNF-α) was significantly higher in the ADM + APS(50 mg/kg),ADM + APS(100 mg/kg) and ADM + APS(200 mg/kg) groups than in the ADM group;and IL-10 was significantly lower in the above groups than in the ADM group.APS could increase IL-1α,IL-2,IL-6,and TNF-α expression and decrease IL-10 levels.Compared with the ADM group,APS treatment at a dose of 50-200 mg/kg could downregulate MDR1 mRNA expression in a dose-dependent manner(0.48 ± 0.13 vs 4.26 ± 1.51,P = 0.000;0.36 ± 0.03 vs 4.26 ± 1.51,P = 0.000;0.21 ± 0.04 vs 4.26 ± 1.51,P = 0.000).The expression level of P-GP was significantly lower in the ADM + APS(200 mg/kg) group than in the ADM group(137.35 ± 9.20 mg/kg vs 282.19 ± 20.54 mg/kg,P = 0.023).CONCLUSION:APS exerts a synergistic anti-tumor effect with ADM in H22 tumor-bearing mice.This may be related to its ability to enhance the expression of IL1α,IL-2,IL-6,and TNF-α,decrease IL-10,and downregulate MDR1 mRNA and P-GP expression levels. | Qing-E Tian Huan-De Li Miao Yan Hua-Lin Cai Qin-You Tan Wen-Yuan Zhang | 2012 | World Journal of Gastroenterology2012，18，47： | 30 |
| 13 | Ultrasonically activated scalpel versus monopolar electrocautery shovel in laparoscopic total mesorectal excision for rectal cancer显示文摘AIM: To investigate the feasibility and safety of monopolar electrocautery shovel (ES) in laparoscopic total mesorectal excision (TME) with anal sphincter preservation for rectal cancer in order to reduce the cost of the laparoscopic operation, and to compare ES with the ultrasonically activated scalpel (US). METHODS: Forty patients with rectal cancer, who underwent laparoscopic TME with anal sphincter preservation from June 2005 to June 2007, were randomly divided into ultrasonic scalpel group and monopolar ES group, prospectively. White blood cells (WBC) were measured before and after operation, operative time, blood loss, pelvic volume of drainage, time of anal exhaust, visual analogue scales (VAS) and surgery-related complications were recorded. RESULTS: All the operations were successful; no one was converted to open procedure. No significant differences were observed in terms of preoperative and postoperative d 1 and d 3 WBC counts (P = 0.493, P = 0.375, P = 0.559), operation time (P = 0.235), blood loss (P = 0.296), anal exhaust time (P = 0.431), pelvicdrainage volume and VAS in postoperative d 1 (P = 0.431, P = 0.426) and d 3 (P = 0.844, P = 0.617) between ES group and US group. The occurrence of surgery-related complications such as anastomotic leakage and wound infection was the same in the two groups. CONCLUSION: ES is a safe and feasible tool as same as US used in laparoscopic TME with anal sphincter preservation for rectal cancer on the basis of the skillful laparoscopic technique and the complete understanding of laparoscopic pelvic anatomy. Application of ES can not only reduce the operation costs but also benef it the popularization of laparoscopic operation for rectal cancer patients. | Bao-Jun Zhou, Wei-Qing Song, Qing-Hui Yan, Jian-Hui Cai, Feng-An Wang, Jin Liu, Guo-Jian Zhang, Guo-Qiang Duan, Zhan-Xue Zhang, Department of Gastrointestinal Surgery, The Second Hospital of Hebei Medical University, Shijiazhuang 050000, Hebei Province, China Author contributions: Zhou BJ, Song WQ, Yan QH, Cai JH, Wang FA, Liu J, Zhang GJ, Duan GQ and Zhang ZX contributed equally to this work Zhou BJ, Song WQ, Yan QH, Cai JH and Wang FA designed the research Zhou BJ, Liu J and Zhang GJ performed the research Duan GQ and Zhang ZX analyzed the data and Zhou BJ, Song WQ and Yan QH wrote the paper. | 2008 | World Journal of Gastroenterology2008，14，25： | 25 |
| 14 | NRS2002 assesses nutritional status of leukemia patients undergoing hematopoietic stem cell transplantation显示文摘Objective:To discuss whether nutritional risk screening 2002(NRS2002) is appropriate for nutritional risk screening for leukemia patients before and after hematopoietic stem cell transplantation(HSCT),and whether there are risk differences in other conditions,such as age,gender and matching degree;to find the methods and indicators of nutritional risk screening for these patients before and after HSCT,in order to give timely intervention to guarantee the successful completion of the entire transplantation process.Methods:Nutritional risk of 99 leukemia patients was screened with NRS2002 before and after HSCT.The χ2 test was applied to compare the risk differences between groups such as age,gender and matching degree,while the differences of other enumeration data,such as recent(1-3 months) weight loss,reduced food intake within one week and BMI,were compared by continuity correction.Results:Of the 99 leukemia patients,22 cases(22.2%) had nutritional risk before HSCT,while all patients had nutritional risk after HSCT;there is no significant difference in nutritional risk between male and female,and patients of less than 30 years old,not-full matched,recent(1-3 months) weight loss,reduced food intake within a week or BMI <18.5 were more likely to have nutritional risk;and 77 cases(77.8%) had weight loss,among which 49 patients(63.6%) had more than 5% weight loss within one month.Conclusions:This study showed that leukemia patients should receive the nutritional risk screening conventionally before and after HSCT,and NRS2002 was only appropriate for nutritional risk screening before HSCT.More attention should be paid to the patients less than 30 years old or not-full matched.Weight change was one of the important nutritional indicators for patients after HSCT. | Peng Liu Zhao-Feng Zhang Jing-Jing Cai Bo-Shi Wang Xia Yan | 2012 | Chinese Journal of Cancer Research2012，24，4： | 25 |
| 15 | Comparison of unipedicular and bipedicular percutaneouskyphoplasty for treating osteoporotic vertebral compression fractures:a meta-analysis显示文摘 | Li Lian-hua Sun Tian-sheng Liu Zhi Zhang Jian-zheng Zhang Yan Cai Yan-hong Wang Hao | 2013 | Chinese Medical Journal2013，，20： | 26 |
| 16 | Basic Soil Productivity of Spring Maize in Black Soil Under Long-Term Fertilization Based on DSSAT Model显示文摘Increasing basic farmland soil productivity has significance in reducing fertilizer application and maintaining high yield of crops. In this study, we defined that the basic soil productivity(BSP) is the production capacity of a farmland soil with its own physical and chemical properties for a specific crop season under local environment and field management. Based on 22-yr(1990-2011) long-term experimental data on black soil(Typic hapludoll) in Gongzhuling, Jilin Province, Northeast China, the decision support system for an agro-technology transfer(DSSAT)-CERES-Maize model was applied to simulate the yield by BSP of spring maize(Zea mays L.) to examine the effects of long-term fertilization on changes of BSP and explore the mechanisms of BSP increasing. Five treatments were examined:(1) no-fertilization control(control);(2) chemical nitrogen, phosphorus, and potassium(NPK);(3) NPK plus farmyard manure(NPKM);(4) 1.5 time of NPKM(1.5NPKM) and(5) NPK plus straw(NPKS). Results showed that after 22-yr fertilization, the yield by BSP of spring maize significantly increased 78.0, 101.2, and 69.4% under the NPKM, 1.5NPKM and NPKS, respectively, compared to the initial value(in 1992), but not significant under NPK(26.9% increase) and the control(8.9% decrease). The contribution percentage of BSP showed a significant rising trend(P<0.05) under 1.5NPKM. The average contribution percentage of BSP among fertilizations ranged from 74.4 to 84.7%, and ranked as 1.5NPKM>NPKM>NPK≈NPKS, indicating that organic manure combined with chemical fertilizers(1.5NPKM and NPKM) could more effectively increase BSP compared with the inorganic fertilizer application alone(NPK) in the black soil. This study showed that soil organic matter(SOM) was the key factor among various fertility factors that could affect BSP in the black soil, and total N, total P and/or available P also played important role in BSP increasing. Compared with the chemical fertilization, a balanced chemical plus manure or straw fertilization(NPKM or NPKS) not only increased the concentrations of soil nutrient, but also improved the soil physical properties, and structure and diversity of soil microbial population, resulting in an iincrease of BSP. We recommend that a balanced chemical plus manure or straw fertilization(NPKM or NPKS) should be the fertilization practices to enhance spring maize yield and improve BSP in the black soil of Northeast China. | ZHA Yan WU Xue-ping HE Xin-hua ZHANG Hui-min GONG Fu-fei CAI Dian-xiong ZHU Ping GAO Hong-jun | 2014 | Journal of Integrative Agriculture2014，13，3： | 25 |
| 17 | Generation of haploid embryonic stem cells from Macaca fascicularis monkey parthenotes显示文摘在从经由单性生殖和男性单性生殖的老鼠的胚胎的干细胞(haESCs ) 启用了在修改基因的动物的哺乳动物的房间和代的基因屏蔽的 haploid 的推导的最近的成功。然而, haESCs 是否能从首领被导出,仍然保持未知。这里,我们从 Macaca fascicularis 猴子的单性生殖的胚囊报导 haESCs 的推导。这些房间,作为 PG-haESCs 称为,是 pluripotent 并且能在 vitro 或在 vivo 区分到三胚胎的细菌层的房间。有趣地,一根猴子 PG-haESC 线(MPH1 ) 的 haploidy 与另一个(MPH2 ) 的相比是更稳定的,作为没有排序的激活荧光的房间(FACS ) ,由 haploid 房间的存在出现超过 140 天 haploid 房间的丰富。重要地,转基因的猴子 PG-haESC 线能被 lentivirus- 和 piggyBac 产生调停 transposon 的基因转移。而且,基因屏蔽在猴子 PG-haESCs 是可行的。我们的结果证明 PG-haESCs 能从猴子被产生,提供为在首领的基因分析的一个理想的工具。 | Hui Yang Zhen Liu Yu Ma Cuiqing Zhong Qi Yin Chikai Zhou Linyu Shi Yijun Cai Hanzhi Zhao Hui Wang Fan Tang Yan Wang Chenchen Zhang Xin-yuan Liu Dongmei Lai Ying Jin Qiang Sun Jinsong Li | 2013 | Cell Research2013，23，10： | 22 |
| 18 | Current status of diagnosis and treatment of bladder cancer in China-Analyses of Chinese Bladder Cancer Consortium database显示文摘Objective:To investigate current status of diagnosis and treatment of bladder cancer in China.Methods:A database was generated by Chinese Bladder Cancer Consortium(CBCC).From January 2007 to December 2012,14,260 cases from 44 CBCC centers were included.Data of diagnosis,treatment and pathology were collected.Results:The average age was 63.5 year-old and most patients were male(84.3%).The most common histologic types were urothelial carcinoma(91.4%),adenocarcinoma(1.8%),and squamous carcinoma(1.9%).According to 1973 and 2004 WHO grading system,42.0%,41.0%,and 17.0% of patients were grade 1,2,and 3,and 16.0%,48.7%,and 35.3% of patients were papillary urothelial neoplasms of low malignant potential,low,and high grade,respectively.Non-muscle invasive bladder cancer(NMIBC)and muscle invasive bladder cancer(MIBC)were 25.2% and 74.1%,respectively(0.8% not clear).Carcinoma in situ was only 2.4%.Most patients were diagnosed by white-light cystoscopy with biopsy(74.3%).Fluorescence and narrow band imaging cystoscopy had additional detection rate of 1.0% and 4.0%,respectively.Diagnostic transurethral resection(TUR)provided detection rate of 16.9%.Most NMIBCs were treated with TUR(89.2%).After initial TUR,2.6%accepted second TUR,and 45.7%,69.9%,and 58.7% accepted immediate,induced,and maintenance chemotherapy instillation,respectively.Most MIBCs were treated with radical cystectomy(RC,59.7%).Laparoscopic RCs were 35.1%,while open RC 63.4%.Extended and standard pelvic lymph node dissection were 7% and 66%,respectively.Three most common urinary diversions were orthotopic neobladder(44%),ileal conduit(31%),and ureterocutaneostomy(23%).Only 2.3% of patients accepted neo-adjuvant chemotherapy and only 18%of T3 and T4 patients accepted adjuvant chemotherapy.Conclusion:Disease characteristics are similar to international reports,while differences of diagnosis and treatment exist.This study can provide evidences for revisions of the guideline on bladder cancer in China. | Kaiwen Li Tianxin Lin 无 Wei Xue Xin Mu Enci Xu Xu Yang Fubao Chen Guangyong Li Lulin Ma Guoliang Wang Chaozhao Liang Haoqiang Shi Ming Li Mao Tang Xueyi Xue Yisong Lv Yaoliang Deng Chengyang Li Zhiwen Chen Xiaozhou Zhou Fengshuo Jin Xudong Liu Jinxin Wei Lei Shi Xin Gou Weiyang He Liqun Zhou Lin Cai Baiye Jin Guanghou Fu Xiangbo Kong Hongyan Sun Ye Tian Lang Feng Tiejun Pan Yiyi Wu Dongwen Wang Hailong Hao Benkang Shi Yaofeng Zhu Qiang Wei Ping Han Changli Wu Dawei Tian Zhangqun Ye Zheng Liu Zhiping Wang Junqiang Tian Lin Qi Minfeng Chen Wei Li Jinchun Qi Gongxian Wang Longlong Fu Zhaolin Sun Guangheng Luo Zhoujun Shen Zhaowei Zhu Jinchun Xing Zhun Wu Dong Wei Xin Chen Yanqun Na Hongfeng Guo Chunxi Wang Zhihua Lu Chuize Kong Yang Liu Jin Yang Jianyun Hu Xin Gao Jielin Li Changjun Yin Pu Li Shan Chen Zhen Du Jiongming Li Yongji Yan Xu Zhang Shuang Huang Fangjian Zhou Zhiling Zhang Yinghao Sun Shuxiong Zeng Song Cen Jiaquan Zhou Hanzhong Li Jin Wen Jian Huang | 2015 | Asian Journal of Urology2015，2，2： | 21 |
| 19 | BMAL1 knockout macaque monkeys display reduced sleep and psychiatric disorders显示文摘Circadian disruption is a risk factor for metabolic, psychiatric and age-related disorders, and non-human primate models could help to develop therapeutic treatments. Here, we report the generation of BMAL1 knockout cynomolgus monkeys for circadian-related disorders by CRISPR/Cas9 editing of monkey embryos. These monkeys showed higher nocturnal locomotion and reduced sleep, which was further exacerbated by a constant light regimen. Physiological circadian disruption was reflected by the markedly dampened and arrhythmic blood hormonal levels. Furthermore, BMAL1-deficient monkeys exhibited anxiety and depression, consistent with their stably elevated blood cortisol, and defective sensory processing in auditory oddball tests found in schizophrenia patients. Ablation of BMAL1 up-regulated transcriptional programs toward inflammatory and stress responses, with transcription networks associated with human sleep deprivation, major depressive disorders, and aging. Thus, BMAL1 knockout monkeys are potentially useful for studying the physiological consequences of circadian disturbance, and for developing therapies for circadian and psychiatric disorders. | Peiyuan Qiu Jian Jiang Zhen Liu Yijun Cai Tao Huang Yan Wang Qiming Liu Yanhong Nie Fang Liu Jiumu Cheng Qing Li Yun-Chi Tang Mu-ming Poo Qiang Sun Hung-Chun Chang | 2019 | National Science Review2019，6，1： | 21 |
| 20 | One-step generation of complete gene knockout mice and monkeys by CRISPR/Cas9-mediated gene editing with multiple sgRNAs显示文摘CRISPR/Cas9 系统是一个有效编辑基因的方法,但是编辑基因的动物的多数显示出 mosaicism,与编辑仅仅在房间的部分发生。这里,我们证明那单个基因或多重基因能被 Cas9 mRNA 和多重邻近的单个指南的 RNA 的 zygotic 注射完全在老鼠和猴子胚胎击倒(spaced 10-200 bp 分开) 指向仅仅一把单个钥匙每基因的 exon。个别地在 Y 染色体上跟随八基因的指向的删除的 F0 老鼠的 Phenotypic 分析在产生猛烈老鼠表明了这条途径的坚韧性。重要地,这条途径在高效率交付完全的基因大美人(Arntl 上的 100% 并且 91% 在 Prrt2 上) 在猴子胚胎。最后,我们能在一个单个步骤产生一只完全的 Prrt2 猛烈猴子,表明在很快建立编辑基因的猴子模型的这条途径的实用性。 | Erwei Zuo Yi-Jun Cai Kui Li Yu Wei Bang-An Wang Yidi Sun Zhen Liu Jiwei Liu Xinde Hu Wei Wei Xiaona Huo Linyu Shi Cheng Tang Dan Liang Yan Wang Yan-Hong Nie Chen-Chen Zhang Xuan Yao Xing Wang Changyang Zhou Wenqin Ying Qifang Wang Ren-Chao Chen Qi Shen Guo-Liang Xu Jinsong Li Qiang Sun Zhi-Qi Xiong Hui Yang | 2017 | Cell Research2017，27，7： | 21 |
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