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| 1 | Structural and molecular features of intestinal strictures in rats with Crohn's-like disease显示文摘AIM: To develop a new rat model we wanted to gain a better understanding of stricture formation in Crohn's disease(CD).METHODS: Chronic colitis was induced locally by the administration of 2,4,6-trinitrobenzenesulfonic acid(TNBS). The relapsing inflammation characteristic to CD was mimicked by repeated TNBS treatments. Animals were randomly divided into control, once, twice and three times TNBS-treated groups. Control animals received an enema of saline. Tissue samples were taken from the strictured colonic segments and also adjacent proximally and distally to its 60, 90 or 120 d after the last TNBS or saline administrations. The frequency and macroscopic extent of the strictures were measured on digital photographs. The structural features of strictured gut wall were studied by light- and electron microscopy. Inflammation related alterations in TGF-beta 2 and 3, matrix metalloproteinases 9(MMP9) and TIMP1 m RNA and protein expression were determined by quantitative real-time PCR and western blot analysis. The quantitative distribution of caspase 9 was determined by post-embedding immunohistochemistry.RESULTS: Intestinal strictures first appeared 60 d after TNBS treatments and the frequency of them increased up to day 120. From day 90 an intact lamina epithelialis, reversible thickening of lamina muscularis mucosae and irreversible thickening of the muscularis externa were demonstrated in the strictured colonic segments. Nevertheless the morphological signs of apoptosis were frequently seen and excess extracellular matrix deposition was recorded between smooth muscle cells(SMCs). Enhanced caspase 9 expression on day 90 in the SMCs and on day 120 also in myenteric neurons indicated the induction of apoptosis. The m RNA expression profile of TGF-betas after repeated TNBS doses was characteristic to CD, TGF-beta 2, but not TGF-beta 3 was up-regulated. Overexpression of MMP9 and down-regulation of TIMP1 were demonstrated. The progressive increase in the amount of MMP9 protein in the strictures was also obvious between days 90 and 120 but TIMP1 protein was practically undetectable at this time.CONCLUSION: These findings indicate that aligned structural and molecular changes in the gut wall rather than neuronal cell death play the primary role in stricture formation. | Petra Talapka Anikó Berkó Lajos István Nagy Lalitha Chandrakumar Mária Bagyánszki László Géza Puskás éva Fekete Nikolett Bódi | 2016 | World Journal of Gastroenterology2016，22，22： | 3 |
| 2 | Risk factors for treatment outcome in fungal keratitis显示文摘 | Lalitha P Prajna N V Kabra A | 2006 | Ophthalmology2006，113，4： | 1 |
| 3 | Differential gene ex-pression profile of the hepatopancreas of white spot syndrome virus infectedFenneropenaeus indicus by suppression subtractive hybridization显示文摘 | JAMES R THAMPURAN N LALITHA K V | 2010 | Fish&Shellfish Immunology2010，29，5： | 1 |
| 4 | Xylan-Degrading Enzymes from the Therrnophilic Fungus Humicola Lanuginosa ( Griffon and Maublanc) Bunce : Action Pattern of Xylanase and β- Glucosidase on Xylans, Xylooligomers and Arabinoxylooligomers 显示文摘 | Lalitha N Paul J V | 1996 | Journal of Fermentation and Bioengineering1996，6，： | 1 |
| 5 | Generalized nonsmooth invexity显示文摘 | Kaul R N Suneja S K Lalitha C S | 1994 | J Info Optim Sci1994，15，： | 1 |
| 6 | Ellagic acid encapsulated chitosan nanoparticles as anti-hemorrhagic agent显示文摘 | Lalitha G Lakshmi N R Swaminathan S | 2014 | Carbohydrate Polymers2014，111，10： | 1 |
| 7 | Effect of packaging atmosphere on the microbial attributes of pearlspot( Etroplus suratensis Bloch)stored at 0 ℃ - 2℃ 显示文摘 | Ravi Sankar C N Lalitha K V Jose L | 2008 | Food Microbiology2008，25，3： | 1 |
| 8 | Effect of chitosan edible coating on the quality of double filleted Indian oil sardine (Sardinella longiceps ) during chilled storage 显示文摘 | Mohan C O Ravishankar C N Lalitha K V | 2012 | Food Hydroco112012，26，1： | 1 |
| 9 | Effects of sodium acetate dip treatment and vacuum-packaging on chemical, microbiological, textural and sensory changes of pearlspot(Etroplus suratensis ) during chill storage 显示文摘 | Manju S Jose L Gopal T K S Ravishankar C N Lalitha K V | 2007 | Food Chemistry2007，102，7： | 1 |
| 10 | Rapid diagnosisof acute pyogenic meningitis by a combined PCR dot-blotassay显示文摘 | Balganesh M Lalitha M K N athaniel R | 2000 | Mol Cell Probes2000，14，2： | 1 |
| 11 | Fuzzy Logic-Retrieval of Data from Database 显示文摘 | Lalitha R V S Pavani N | 2011 | Computer Engineer and Intelligent Systems2011，2，8： | 1 |
| 12 | Effect of chitosan edible coating on the quality of double filleted Indian oil sardine (Sardinel lalongicep') during chilled storage显示文摘 | MOHAN C O RAVISHANKAR C N LALITHA K V | 2011 | Food Hydrocolloids2011，，： | 1 |
| 13 | Infections in immtmocomprowised host: aprospectivc multicenter survey in patients receving chemotherapy for acute leukemia显示文摘 | Advani SH Kochupillai V Lalitha N etal | 1996 | J Assco Physicians India1996，44，11： | 1 |
| 14 | Boards: Does one size fit all? 显示文摘 | Jeffrey C Naveen D Lalitha N | 2008 | Journal of Financial Economics2008，87，2： | 1 |
| 15 | Iodine-cata-lyzed oneVpot Synthesis of Amides from Nitriles Via Ritter Reaction显示文摘 | Theerthagiri P Lalitha A Arunachalam P N | 2010 | Tetrahedron Letters2010，51，21： | 1 |
| 16 | Effect of packaging atmosphere on the microbial attributes of pearlspot (Elroplus sttratensis Bloch) stored at 0-2℃ 显示文摘 | Ravi Sankar C N Lalitha K V Jose L | 2009 | Food Microbiology2009，25，3： | 1 |
| 17 | Utilization of meat industry by products: Protein hydrolysate from sheep visceral mass 显示文摘 | Bhaskar N Modi V K Govindaraju K Radha C Lalitha R G | 2007 | Bioresource Technology2007，98，： | 1 |
| 18 | Effect ofpackaging atmosphere on the microbial attributes of pearlspot {Etroplussuratensis Bloch)stored at 0-2 °C显示文摘 | RAVI SANKAR C N LALITHA K V JOSE L | 2009 | Food Microbiology2009，25，3： | 1 |
| 19 | Generalized non-smooth invexity显示文摘 | SUNEJA S K LALITHA C S | 1994 | J Information Optimization Science1994，15，： | 1 |
| 20 | Staphylococcus aureus eye infections in two Indian hospitals: emergence of ST772 as a major clone显示文摘 | Nadig S Velusamy N Lalitha P etal | 2012 | Clin Ophthalmol2012，6，： | 1 |
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