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| 1 | Inhibition of SARS-CoV-2 (previously 2019-nCoV) infection by a highly potent pan-coronavirus fusion inhibitor targeting its spike protein that harbors a high capacity to mediate membrane fusion显示文摘The recent outbreak of coronavirus disease(COVID-19)caused by SARS-CoV-2 infection in Wuhan,China has posed a serious threat to global public health.To develop specific anti-coronavirus therapeutics and prophylactics,the molecular mechanism that underlies viral infection must first be defined.Therefore,we herein established a SARS-CoV-2 spike(S)protein-mediated cell-cell fusion assay and found that SARS-CoV-2 showed a superior plasma membrane fusion capacity compared to that of SARS-CoV.We solved the X-ray crystal structure of six-helical bundle(6-HB)core of the HR1 and HR2 domains in the SARS-CoV-2 S protein S2 subunit revealing that several mutated amino acid residues in the HR1 domain may be associated with enhanced interactions with the HR2 domain.We previously developed a pan-coronavirus fusion inhibitor,EK1,which targeted the HR!domain and could inhibit infection by divergent human coronaviruses tested,including SARS-CoV and MERS-CoV.Here we generated a series of lipopeptides derived from EK1 and found that EK1C4 was the most potent fusion inhibitor against SARS-CoV-2 S protein-mediated membrane fusion and pseudovirus infection with IC50s of 1.3 and 15.8 nM,about 241-and 149-fold more potent than the original EK1 peptide,respectively.EK1C4 was also highly effective against membrane fusion and infection of other human coronavirus pseudoviruses tested,including SARS-CoV and MERS-CoV,as well as SARSr-CoVs,and potently inhibited the replication of 5 live human coronaviruses examined,including SARS-CoV-2.Intranasal application of EK1C4 before or after challenge with HCoV-OC43 protected mice from infection,suggesting that EK1C4 could be used for prevention and treatment of infection by the currently circulating SARS-CoV-2 and other emerging SARSr-CoVs. | Shuai Xia Meiqin Liu Chao Wang Wei Xu Qiaoshuai Lan Siliang Feng Feifei Qi Linlin Bao Lanying Du Shuwen Liu Chuan Qin Fei Sun Zhengli Shi Yun Zhu Shibo Jiang Lu Lu | 2020 | Cell Research2020，30，4： | 81 |
| 2 | Characterization of the receptor-binding domain(RBD)of 2019 novel coronavirus:implication for development of RBD protein as a viral attachment inhibitor and vaccine显示文摘The outbreak of Coronavirus Disease 2019(COVID-19)has posed a serious threat to global public health,calling for the development of safe and effective prophylactics and therapeutics against infection of its causative agent,severe acute respiratory syndrome coronavirus 2(SARS-CoV-2),also known as 2019 novel coronavirus(2019-nCoV).The CoV spike(S)protein plays the most important roles in viral attachment,fusion and entry,and serves as a target for development of antibodies,entry inhibitors and vaccines.Here,we identified the receptor-binding domain(RBD)in SARS-CoV-2 S protein and found that the RBD protein bound strongly to human and bat angiotensin-converting enzyme 2(ACE2)receptors.SARS-CoV-2 RBD exhibited significantly higher binding affinity to ACE2 receptor than SARS-CoV RBD and could block the binding and,hence,attachment of SARS-CoV-2 RBD and SARS-CoV RBD to ACE2-expressing cells,thus inhibiting their infection to host cells.SARS-CoV RBD-specific antibodies could crossreact with SARS-CoV-2 RBD protein,and SARS-CoV RBD-induced antisera could cross-neutralize SARS-CoV-2,suggesting the potential to develop SARS-CoV RBD-based vaccines for prevention of SARS-CoV-2 and SARS-CoV infection. | Wanbo Tai Lei He Xiujuan Zhang Jing Pu Denis Voronin Shibo Jiang Yusen Zhou Lanying Du | 2020 | Cellular & Molecular Immunology2020，17，6： | 35 |
| 3 | The Hardy Rubber Tree Genome Provides Insights into the Evolution of Polyisoprene Biosynthesis显示文摘Eucommia ulmoides,也叫的强壮的橡胶树,是一棵经济地重要的树;然而,它的染色体顺序的缺乏 ?限制基本生物研究和这植物种的应用研究。这里,我们在场它的 1.2-Gb 染色体的一个高质量的集会(支架 N50 ?=? 1.88 Mb ) 与至少 26 ? 为 E 的 723 预言的基因。ulmoides,顺序 Garryales 的首先定序的染色体,它用联合定序的 Illumina 的综合策略被获得,定序的 PacBio,和印射的 BioNano。作为到 lamiids 和 campanulids 的姐妹 taxon, E。ulmoides 经历了三倍由核心 eudicots 而是没有进一步整个染色体的复制分享了的一个古老的染色体 ? 在最后 1.25 亿年里。E。ulmoides 为涉及压力回答和第二等的代谢物的生合成的多重基因展出高表达式层次或基因数字扩大,它可以说明它的可观的环境适应性。与橡胶树(Hevea brasiliensis ) 相对照,它生产 cis 聚异式戊二我烯, E。ulmoides 演变综合经由 farnesyl diphosphate synthases (FPS ) 的长链的 trans 聚异式戊二我烯。而且, FPS 和橡胶延伸 factor/small 橡胶粒子蛋白质基因家庭从 H 独立地被扩展。brasiliensis 系。这些结果提供新卓见进 E. 的生物学 ? ulmoides 和聚异式戊二我烯生合成的起源。 | Ta-na Wuyun Lin Wang Huimin Liu Xuewen Wang Liangsheng Zhang Jeffrey L. Bennetzen Tiezhu Li Lirong Yang Panfeng Liu Lanying Du Lu Wang Mengzhen Huang Jun Qing Iili Zhu Wenquan Bao Hongguo Li Qingxin Du Jingle Zhu Hong Yang Shuguang Yang Hui Liu Hui Yue Jiang Hu Suoliang Yu Yu Tian Fan Liang Jingjing Hu Depeng Wang Ruiwen Gao Dejun Li Hongyan Du | 2018 | Molecular Plant2018，11，3： | 24 |
| 4 | Identification of an ideal adjuvant for receptor-binding domain-based subunit vaccines against Middle East respiratory syndrome coronavirus显示文摘 | Naru Zhang Rudragouda Channappanavar Cuiqing Ma Lili Wang Jian Tang Tania Garron Xinrong Tao Sumaiya Tasneem Lu Lu Chien-Te K Tseng Yusen Zhou Stanley Perlman Shibo Jiang Lanying Du | 2016 | Cellular & Molecular Immunology2016，13，2： | 12 |
| 5 | Roles of the hemagglutinin of influenza A virus in viral entry and development of antiviral therapeutics and vaccines显示文摘Seasonal influenza epidemics and influenza pandemics caused by influenza A virus(IAV)has resulted in millions of deaths in the world.The development of anti-IAV vaccines and therapeutics is urgently needed for prevention and treatment of IAV infection and for controlling future influenza pandemics.Hemagglutinin(HA)of IAV plays a critical role in viral binding,fusion and entry,and contains the major neutralizing epitopes.Therefore,HA is an attractive target for developing anti-IAV drugs and vaccines.Here we have reviewed the recent progress in study of conformational changes of HA during viral fusion process and development of HA-based antiviral therapeutics and vaccines. | Shibo Jiang Runming Li Lanying Du Shuwen Liu | 2010 | Protein & Cell2010，1，4： | 5 |
| 6 | A Complete Embryonic Developmental Table of Microhyla fissipes(Amphibia,Anura,Microhylidae)显示文摘Access to embryonic developmental stages is essential basic work for understanding how organisms develop. In this study, seven egg clutches(range 209–564 eggs) of ornamented pygmy frog Microhyla fissipes(Amphibia, Anura, Microhylidae) were obtained from seven breeding pairs in laboratory. One egg clutch of them was observed for the embryonic development, and the staging table of normal development was constructed based on morphological and physiological characteristics. Forty-five developmental stages were defined for M. fissipes, and two major developmental periods were designated: 1) early embryonic development period(stages 1–28), from fertilization to operculum completion stage, lasted for 82.6 hours at water temperature(WT) 23–25℃; 2) larval development period(stages 29–45), from operculum completion to tail complete absorption stage, took 38 days at WT 22–26.5℃, showing that the embryos of this species develop rapidly. In addition, the tadpoles were transparent, which is similar to those in field. These characteristics suggest that M. fissipes would be a good model to study developmental biology, adaptive mechanisms from aquatic to terrestrial phases, environmental toxicology, and human disease. | Shouhong WANG Lanying ZHAO Lusha LIU Dengwei YANG Janak Raj KHATIWADA Bin WANG Jianping JIANG | 2017 | Asian Herpetological Research2017，18，2： | 5 |
| 7 | A novel receptor-binding domain (RBD)-based mRNA vaccine against SARS-CoV-2显示文摘Dear Editor,The pandemic of coronavirus disease 2019(COVID-19)caused by severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)highlights the need to develop effective and safe vaccines.Similar to SARS-CoV,SARS-CoV-2 recognizes angiotensin-converting enzyme 2(ACE2)as receptor for host cell entry.1,2 SARS-CoV-2 spike(S)protein consists of S1,including receptor-binding domain(RBD),and S2 subunits.3,4 We previously demonstrated that RBDs of SARS-CoV and MERS-CoV serve as important targets for the development of effective vaccines. | Wanbo Tai Xiujuan Zhang Aleksandra Drelich Juan Shi Jason CHsu Larry Luchsinger Christopher D.Hillyer Chien-Te KTseng Shibo Jiang Lanying Du | 2020 | Cell Research2020，30，10： | 5 |
| 8 | Cross-neutralization of SARS coronavirus-specific antibodies against bat SARS-like coronaviruses显示文摘Dear Editor,The 2002–2003 global pandemic caused by severe acute respiratory syndrome coronavirus(SARS-CoV)infected around 8,000 people with 10%mortality(http://gffzz36b398b81f8a4a4chw9n6qopf9bf96pq6.ffgz.tsg.suse.edu.cn/csr/sars/en/).The virus has a positivestranded RNA genome that encodes a large polyprotein(1a and 1ab),four structural proteins,including spike(S), | Lei-Ping Zeng Xing-Yi Ge Cheng Peng Wanbo Tai Shibo Jiang Lanying Du Zheng-Li Shi | 2017 | Science China(Life Sciences)2017，60，12： | 4 |
| 9 | A core–shell amidoxime electrospun nanofiber affinity membrane for rapid recovery Au(Ⅲ) from water显示文摘An affinity membrane was prepared by coaxial electrospinning and amidoxime(AONFA),and it was applied to selectively recovery Au(Ⅲ)from an aqueous solution.The static adsorption results showed that,when p H at 5,the maximum adsorption capacity of AONFA membrane for Au(Ⅲ)was 509.3 mg·g^(-1).AONFA membrane exhibit much higher affinity and selectivity towards Au(Ⅲ)than other metal cations.The membrane could be regenerated effectively by mixture solution of thiourea and HCl,and the desorption ratio reached almost 100%after 4 hours desorption.The dead-end filtration results showed that,the membrane utilization efficiency and adsorption capacity can be improved by increasing the flow rate,while increasing the concentration shorted the breakthrough process and had little impact to adsorption capacity.We can flexibly adjust the flow rate and concentration according to the situation to obtain the maximum utilization efficiency of the membrane in filtration process.The dynamic adsorption capacity is higher than the static adsorption capacity.The adsorption mechanism for Au(Ⅲ)is electrostatic adsorption and reduction.Thus,AONFA membrane filtration was demonstrated to be a promising method for continuous recover Au(Ⅲ)from wastewater. | Yang Chen Lanying Jiang | 2022 | Chinese Journal of Chemical Engineering2022，35，2： | 2 |
| 10 | Novel polysiloxaneimide / polyetherimide non-woven fabric composite membranes for organophilic pervaporation显示文摘 | Song Zhengwei Zhu Jiaming Jiang Lanying | 2014 | Journal of Membrane Science2014，472，： | 1 |
| 11 | Investigation of the fundamental differences between polyamide-imide (PAl) and polyetherimide (PEI) membranes for isopropanol dehydration via pervaporation显示文摘 | Yan Wang Lanying Jiang Takeshi Matsuura | | 0，，1： | 1 |
| 12 | Polyimides membranes for pervaporation and biofuels separation显示文摘 | Lanying Jiang Yan Wang Tai-Shung Chung | | 0，，11： | 1 |
| 13 | Research of processing techniques used in fabrication of solid oxide fuel cells and study of their selected properties显示文摘 | Jiang Liu Zhe Lü Wei Liu Li Pei Xin Zhao Li Jia Yanbin Song Xiaomei Liu Lanying He Wenhui Su | 1998 | Science in China Series A: Mathematics1998，，5： | 1 |
| 14 | Clinical and genetic characteristics of Chinese patients with hereditary haemorrhagic telangiectasia–associated pulmonary hypertension显示文摘 | Yan‐Jun Chen Qing‐Hui Yang Dong Liu Qian‐Qian Liu Mélanie Eyries Liang Wen Wen‐Hui Wu Xin Jiang Ping Yuan Rui Zhang Florent Soubrier Zhi‐Cheng Jing | 2013 | Eur J Clin Invest2013，，10： | 1 |
| 15 | Influence of flocculant polyacrylamide on concentration of titanium white waste acid by direct contact membrane distillation显示文摘Effort has been made to investigate the concentration of titanium white waste acid(TWWA) by direct contact membrane distillation(DCMD) and the focus is the influence of polyacrylamide(PAM) on membrane wetting and fouling.It was found that the presence of PAM in feed reduced membrane flux and retarded wetting development,even though its level in feed of H2 SO4 solution was only 0.0002 wt%.The difference between the tests using pure water and neat PAM aqueous solution,respectively,as feed also indicated the above effect registered with PAM.AFM results showed that the addition of PAM in feed solution changed the membrane shell surface morphology.However,conclusive proof of the PAM existence in membrane cross-section and inner surface was not obtained,probably due to its low concentration in feed solution.Two approaches were tried to recover membrane flux.In comparison with operation w/o any treatment,HCl solution rinsing did not lead to obvious improvement in membrane flux recovery,while rinsing combined with gas purge has clearly delayed the degradation in acid concentration factor.Nevertheless,even the latter could not ensure long term stability.High tortuosity and low hydrophobicity of the membranes used were suggested as underlying causes and should be addressed in the future. | Biao Hu Jintao Ouyang Lanying Jiang | 2020 | Chinese Journal of Chemical Engineering2020，28，9： | 1 |
| 16 | Research anddevelopment of universal influenza vaccines显示文摘 | Lanying Du Yusen Zhou Shibo Jiang | 2010 | Mi-crobes and Infection2010，12，： | 1 |
| 17 | Polyimides membranes for pervaporation and biofuels separation显示文摘 | JIANG Lanying WANG Yan CHUNG T S | 2009 | Progress in Polymer Science2009，34，11： | 1 |
| 18 | Investigation of the fundamental differences between polyamideimide(PAI)and polyetherimide(PEI)membranes for isopropanol dehydration via pervaporation显示文摘 | WANG Yan JIANG Lanying MATSUURA T | 2008 | J Membr Sci2008，318，12： | 1 |
| 19 | An investigation to revitalize the separation performance of hollow fibers with a thin mixed matrix composite skin for gas separation显示文摘 | Jiang Lanying Chung Taishung Kulprathipanja S | 2006 | Journal of Membrane Science2006，276，12： | 1 |
| 20 | Advances in the research and development of therapeutic antibodies against the Zika virus显示文摘The Zika virus(ZIKV)is an arbovirus in the same family,Flaviviridae,as the dengue virus(DENV),the West Nile virus,the yellow fever virus,and the Japanese encephalitis virus.ZIKV has been a recent research focus because of its close association with severe human diseases and syndromes,particularly congenital Zika syndrome,which includes fetal microcephaly and other brain abnormalities.1 The ZIKV envelope(E)protein is a major structural protein involved in receptor binding,viral entry,and fusion.It is arranged as a dimer,with each monomer consisting of three domains(DI,DII,and DIII),a stem region,and a transmembrane region.2 The ZIKV E protein induces the production of potent neutralizing antibodies against ZIKV and is thus a key target for the development of therapeutic ZIKV antibodies. | Shibo Jiang Lanying Du | 2019 | Cellular & Molecular Immunology2019，16，1： | 0 |
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