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| 1 | phosphatase and tensin homolog is a differential diagnostic marker between nonalcoholic and alcoholic fatty liver disease显示文摘AIM: To investigate the protein expression of phosphatase and tensin homolog(PTEN) in human liver biopsies of patients with alcoholic and non-alcoholic liver disease.METHODS: PTEN protein expression was assessed by immunohistochemistry in formalin-fixed, paraffinembedded liver sections of patients with non-alcoholic fatty liver disease(NAFLD)(n = 44) or alcoholic liver disease(ALD)(n = 25). Liver resections obtained from 3 healthy subjects candidate for partial liver donation served as controls. Histological evaluations were performed by two experienced pathologists, and diagnoses established based on international criteria. The intensity of the PTEN staining in nuclei was compared between steatotic and non-steatotic areas of each liver fragment analyzed. For each liver specimen, the antibody-stained sections were examined and scored blindly by three independent observers, who were unaware of the patients' clinical history.RESULTS: In healthy individuals, PTEN immunostaining was intense in both the cytoplasm and nuclei of all hepatocytes. However, PTEN was strongly downregulated in both the nucleus and the cytoplasm of hepatocytes from steatotic areas in patients with NAFLD, independently of the disease stage. In contrast, no changes in PTEN protein expression were observed in patients with ALD, regardless of the presence of steatosis or the stage of the disease. The degree of PTEN downregulation in hepatocytes of patients with NAFLD correlated with the percentage of steatosis(r = 0.3061, P = 0.0459) and the BMI(r = 0.4268, P = 0.0043). Hovewer, in patients with ALD, PTEN expression was not correlated with the percentage of steatosis with or without obesity as a confounding factor(P = 0.5574). Finally, PTEN expression level in steatotic areas of ALD patients was significantly different from that seen in steatotic areas of NAFLD patients(P < 0.0001).CONCLUSION: PTEN protein expression is downregulated early in NAFLD, but not in ALD. PTEN immunohistochemical detection could help in the differential diagnosis of NAFLD and ALD. | Andrea Sanchez-Pareja Sophie Clément Marion Peyrou Laurent Spahr Francesco Negro Laura Rubbia-Brandt Michelangelo Foti | 2016 | World Journal of Gastroenterology2016，22，14： | 2 |
| 2 | Hepatic cell proliferation plays a pivotal role in the prognosis of alcoholic hepatitis显示文摘 | Nicolas Lanthier Laura Rubbia-Brandt Nathalie Lin-Marq Sophie Clément Jean-Louis Frossard Nicolas Goossens Antoine Hadengue Laurent Spahr | 2015 | Journal of Hepatology2015，，3： | 1 |
| 3 | Collagen proportionate area correlates to hepatic venous pressure gradient in non-abstinent cirrhotic patients with alcoholic liver disease显示文摘AIM To explore the relationship between collagen proportionate area(CPA) and portal hypertension-related clinical manifestations in alcoholic liver disease(ALD).METHODS Retrospective study with chart review of patients with ALD adressed to our center between January 2012 and December 2013 for a transjugular liver biopsy(TJLB) and hepatic hemodynamic study. Patients were included if they met the following criteria:(1) Medical indication for a liver biopsy in the setting of ALD;(2) recent(< 15 d) clinical, radiological, endoscopic and biological data available; and(3) estimated follow-up of at least 6 mo. Liver tissue from cirrhotic subjects obtained from transjugular liver biopsies was stained with Picro Sirius red and computer-assisted digital image analysis to determine fibrosis density using CPA was performed. RESULTS We included 61 patients with alcoholic ALD, subdivided in 41 active alcohol drinkers and 20 durably abstinent patients. Nine healthy liver donors served as controls. Mean CPA in patients with ALD was 7.1%, with no difference between active drinkers and abstinent patients(P = 0.17). Using a fibrosis density cutoff of 5%, we observed a positive correlation between high fibrosis density and the hepatic venous pressure gradient(HVPG) only in active drinkers(P = 0.02). At 12-mo of follow-up, in the group of active alcohol drinkers, patients reaching a composite outcome showed a higher HVPG value as compared to those who did not(18.5 mm Hg vs 14.5 mm Hg P < 0.04) whereas CPA values were similar(6.9% vs 11%, P = 0.23).CONCLUSION In active alcoholic ALD, CPA correlates to portal pressure but only HVPG predicts clinical events, pointing to the role of alcohol as a modulator of portal hypertension. | Sophie Restellini Nicolas Goossens Sophie Clément Nicolas Lanthier Francesco Negro Laura Rubbia-Brandt Laurent Spahr | 2018 | World Journal of Hepatology2018，10，1： | 1 |
| 4 | Human Herpesvirus-8 Oral Shedding in Amerindian Populations in the Brazilian Amazon Region显示文摘 | Laura Masami Sumita Wilton Santos Freire Mariana Quiroga Maria Cristina D.S. Fink Cynthia L.M. Canto Tania Regina Tozetto Mendoza Paulo Henrique Braz Silva Cláudio Sérgio Pannuti | 2015 | Oral Surgery, Oral Medicine, Oral Pathology and O2015，，： | 1 |
| 5 | Central pontine myelinolysis in a previously healthy 4-year-old child with acute rotavirus gastroenteritis 显示文摘 | Laura G Sutton CL | 1997 | Pediatrics1997，99，5： | 1 |
| 6 | Availability assessment to improve public health policies, programs, and practices 显示文摘 | Laura CL aura KK Debra R | 2010 | Annual Review of Public Health2010，31，： | 1 |
| 7 | MYB10 playsa major role in the regulation of flavonoid/phenylpropanoidmetabolism during ripening of Fragaria x anartassa fruits显示文摘 | Laura MP Guadalupe CL Francisco AR’ | 2014 | Journal of Experimental Botany2014，65，2： | 1 |
| 8 | Differentiation of classical swine fever virus from ruminant pestiviruses by reverse transcription and polymerase chain reaction(RT- PCR) 显示文摘 | Clá udio W C Isidro H Laura L A | 1996 | Veterinary Microbiol1996，48，： | 1 |
| 9 | Guidelines for the design and analysis of studies on NSCLP in humans 显示文摘 | Laura EM Terri HB Andrew CL | 2002 | Cleft Palate Craniofac J2002，39，1： | 1 |
| 10 | Developing a protocol based on the Brazilian Dietary Guidelines for individual dietary advice in the primary healthcare:theoretical and methodological bases显示文摘To describe the methodology of development of a protocol for application of the Brazilian Dietary Guidelines by primary healthcare professionals in individual dietary advice.A five-step approach was followed:(1)format definition;(2)definition of the instrument for assessment of individuals’food consumption;(3)Dietary Guidelines’content extraction;(4)protocol content development;(5)content and face validity.An example from Brazil was displayed with the development of a protocol to guide healthcare professional decision-making when providing nutrition advice based on the Brazilian Dietary Guidelines.The instrument of the Brazilian Nutrition Surveillance System(SISVAN)was chosen to the food consumption assessment,which contains questions about the consumption of seven healthy or unhealthy food groups and one question about eating modes.The Guidelines’content extraction process led to the identification of recommendations related to the food consumption markers assessed by the SISVAN questionnaire.Then,a protocol was developed in a flowchart format,in which the professional’s conduct is guided by the answer given to each question of the SISVAN instrument.For each‘non-compliant’answer(unhealthy eating practice),the professional is instructed how to provide recommendations and identify obstacles.Lastly,experts and healthcare professionals highlighted pertinence,clarity and usability of the protocol.This study provides the blueprint for the phase-wise development of protocols of application of the Dietary Guidelines and may contribute to promote healthier eating and ending malnutrition in all its forms. | Maria Laura da Costa Louzada Cláudia Raulino Tramontt Juliana Giaj Levra de Jesus Fernanda Rauber Jacqueline Resende Berriel Hochberg Thanise Sabrina Souza Santos Patricia Constante Jaime | 2022 | Family Medicine and Community Health2022，10，1： | 0 |
| 11 | Disordered eating behaviour and eating disorder among adolescents with type 1 diabetes:An integrative review显示文摘BACKGROUND Type 1 diabetes(DT1)in adolescents brings behavioural changes,altered nutritional habits,and eating disorders.AIM To identify and analyze the validated instruments that examine the disordered eating behaviour and eating disorders among adolescents with DT1.METHODS An integrative review was accomplished based on the following databases:PubMed,LILACS,CINAHL,Scopus,Web of Science,and Reference Citation Analysis(RCA),including publications in Portuguese,English,or Spanish,without time limit and time published.RESULTS The main instruments to evaluate disordered eating behaviour were The Diabetes Eating Problem Survey-Revised,The Diabetes Eating Problem Survey,and the eating attitudes test-26,and for eating disorders the main instruments used were The Bulimic Investigation Test of Edinburgh,The Binge Eating Scale,The Child Eating Disorder Examination,The five questions of the(Sick,Control,One,Fat and Food),and The Mind Youth Questionnaire.These instruments showed an effect in evaluating risks regarding nutritional habits or feeding grievances,with outcomes related to weight control,inadequate use of insulin,and glycaemia unmanageability.We did not identify publication bias.CONCLUSION Around the world,the most used scale to study the risk of disordered eating behaviour or eating disorder is The Diabetes Eating Problem Survey-Revised.International researchers use this scale to identify high scores in adolescents with DT1 and a relationship with poorer glycemic control and psychological problems related to body image. | Maria Conceição Santos Oliveira Cunha Francisco Clécio Silva Dutra Laura Martins Mendes Cavaleiro Brito Rejane Ferreira Costa Maria Wendiane Gueiros Gaspar Danilo Ferreira Sousa Márcio Flávio Moura deAraújo Maria Veraci Oliveira Queiroz | 2022 | World Journal of Meta-Analysis2022，10，5： | 0 |
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