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| 1 | Cloning and expression of MXR7 gene in human HCC tissue显示文摘AIM To clone and identify the whole cDNA ofMXR7 gene and to find out its expression inhuman HCC,and normal tissues.METHODS The DNA primers were designed andsynthesized according to the whole cDNAsequence of MXR? gene.The cDNA of humanHCC was taken as the template while the cDNA ofMXR7 gene was synthesized by polymerasechain reaction(PCR).Recombinant DNAconforming to reading frame was constructed byconnecting purified PCR product of the cDNA ofMXR? gene with expression vector pGEX-5X-1 offusion protein.The plasmid MXRT/pGEX-5X-1was identified by sequencing.Using 32p labeledMXR? cDNA as probe,MXR7 mRNA expressionwas detected by Northern blot analysis in 12different human normal tissues,7 preoperativelyuntreated non-liver tumor tissues,30preoperatively untreated HCC,theparacancerous liver tissues and 12 normal livertissues samples.RESULTS Restriction enzyme and sequenceanalysis confirmed that the insertion sequence invector pGEX-5X-1 was the same as the cDNAsequence of MXR7 gene.Northern blot analysisshowed no expression of MXR? mRNA in 12 kindsof normal human tissues including liver,7 tumortissues in other sites and 12 normal livertissues,the frequencies of MXR7 mRNA expression in HCC and paracancerous livertissues were 76.6% and 13.3%,respectively.The frequency of MXR7 mRNA expression in HCCwithout elevation of serum AFP and in HCC<5cm was 90%(9/10)and 83.3%(5/6),respectively.CONCLUSION MXR7 mRNA is highly expressedin human HCC,which is specific and occurs at anearly stage of HCC,suggesting MXR7 mRNA canbe a tumor biomarker for HCC.The detection ofMXR7 mRNA expression in the biopsied livertissue is helpful in discovering early subclinicalliver cancer in those with negative serum AFP. | Zhou XP Wang HY Yang GS Chen ZJ Li BA Wu MC | 2000 | World Journal of Gastroenterology2000，6，1： | 23 |
| 2 | Effects of Sucrose Concentration on the Developmental Potential of Human Frozen-Thawed Oocytes at Different Maturity Stages显示文摘 | Chen ZJ Li M Li Y | 2004 | Hum Reprod2004，19，： | 2 |
| 3 | Gaps remain in China’s ability to detect emerging infectious diseases despite advances since the onset of SARS and avian flu显示文摘 | Feng ZJ Li WK Varma JK | 2010 | Health Affairs2010，30，1： | 1 |
| 4 | Peptides as targeting probes against tumor vasculature tr diagnosis and drug delivery显示文摘 | Li ZJ Cho CH | 2012 | i Transl Med2012，10，1： | 1 |
| 5 | Aldose reductase deficiency reducedvascular changes in neonatal mouse retina in oxygen-induced retinop-athy显示文摘 | Fu ZJ Li SY Kociok N | 2012 | Invest Ophthalmol Vis Sci2012，53，9： | 1 |
| 6 | CCL19-induced chemokine receptor 7 activates the phosphoinositide-3 kinase-mediated invasive pathway through Cdc42 in metastatic squamous cell carcinoma of the head and neck显示文摘 | Zhao ZJ Liu FY Li P | 2011 | Oncol Rep2011，25，3： | 1 |
| 7 | Individual function difference of Runx2 isoforms in bone formation by RNA inferen显示文摘 | Li YL INi ZJ Zheng SP | 2008 | Cell Biol Int2008，32，3： | 1 |
| 8 | Diagnosisandtreatmentofhepaticcholangiocarcinoma: report of 52 cases 显示文摘 | Guo ZJ Li Q HeK | 2013 | Hepatobiliary Pancreat Dis Int2013，2，1： | 1 |
| 9 | Investigation of the electrochemical and electrocatalytic behavior of single-wall carbon nanotube film on a glassy carbon electrode显示文摘 | Luo HX Shi ZJ Li NQ | 2001 | Anal Chem2001，73，： | 1 |
| 10 | Three-dimensional reconstruction of registered and fused Chinese visible human and Patient MRI Images 显示文摘 | Li L Liu YX Song ZJ | 2006 | Clin Anat2006，19，3： | 1 |
| 11 | Pancreaticoduodenectomy with preoperative obstructive jaundice drainage or not 显示文摘 | Li ZJ Zhang ZD Hu WM | 2009 | Pancreas2009，38，： | 1 |
| 12 | Disease burden of chronic ob-structive pulmonary diseases in west rural areas of China,2004-2005显示文摘 | Shi XQ Hu N Li XY Huang ZJ | 2011 | Zhonghua Yu Fang Yi Xue Za Zhi2011，45，1： | 1 |
| 13 | Delayed drainage versus auto- transfusion drainage and routine drainage after total knee arthro- plasty: a comparative study显示文摘 | Zhang Y Li ZJ Zheng YF | 2013 | J Orthop Surg Res2013，8，： | 1 |
| 14 | The effect of innate immunity on autoimmune diabetes and the expression of Toll-like receptors on pancreatic islets 显示文摘 | Wen L Peng J Li ZJ | 2004 | J Immunol2004，172，5： | 1 |
| 15 | Percutaneous injection of intradiscal an Parasplnal space with O2-O3mixture to treat lumbar disc herniation显示文摘 | He XF Yu ZJ Li YH | 2003 | Rivista Italian adiOssigeno-Ozono terapia2003，2，2： | 1 |
| 16 | Pentacyclic triterpenoids from Rubus xanthocarpus 显示文摘 | Li BZ Wang BG Jia ZJ | 1998 | Phytochemistry1998，49，8： | 1 |
| 17 | Identification and functional characterization of a novel interleukin 17 receptor:a possible mitogenic activation throughras/mitogen-activated protein kinase signaling pathway显示文摘 | Li TS Li XN Chang ZJ | 2006 | Cell signal2006，18，8： | 1 |
| 18 | MiR-21 plays a pivotal role in gastric cancer pathogenesis and progression显示文摘 | Zhang ZY Li ZJ Gao CP | 2008 | Lab Invest2008，88，12： | 1 |
| 19 | Improved vitamin B(12) production by step-wise reduction of oxygen uptake rate under dissolved oxygen limiting level during fermentation process显示文摘 | Wang ZJ Wang HY Li YL | 2010 | Bioresource Technology2010，101，8： | 1 |
| 20 | Characterization of the E-138 (Glu/Lys) mutation in Japanese encephalitis virus by using a stable, full-length, infectious cDNA clone 显示文摘 | Zhao ZJ Date T Li YH | 2005 | J Gen Virol2005，86，8： | 1 |
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