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| 1 | Heavy metal concentrations of agricultural soils and vegetables from Dongguan, Guangdong显示文摘118 农业土壤和 43 件蔬菜样品的一个总数从 Dongguan 城市被收集,广东,中国。空间分发,来源,累积特征和在农业土壤和蔬菜的重金属的潜在的风险被三条不同途径在细节描绘,包括在土壤和蔬菜的八个金属元素的全部的内容,在在学习的土壤的重金属的 GIS 地图和 multivariate 分析。结果证明在农业土壤有象 Cu , Zn , Ni , Pb , Cd 和 Hg 那样的重金属的更高的累积,并且 Pb 的内容( 65.38 mg kg ? 2 )并且 Hg ( 0.24 mg kg ? 2 )分别地是在广东省的土壤的相应重金属的背景内容的 1.82 和 2.82 次。有在附近的 3.4% Cu, 5.9% Ni, 1.7% Cd 和 28% Hg 在所有从有的所有调查地点收集了土壤样品溢出为为土壤标准的中国环境质量的重金属的内容(GB15618-1995,等级 II ) 。在土壤的多金属的污染特征被 Hg 主要反映。到在土壤的八个金属元素有不同来源, Cu, Zn, Ni, Cr 并且这是主要源于父母材料,并且 Pb, Hg 和 Cd 被人为的活动影响。空间分发表演 Cu, Zn, Ni, Cr, Pb 并且农业土壤的 Hg 内容在西方高并且在东方,和 Cd 内容低在西北高,东南并且在在 Dongguan 的西南低。Ni 的蔬菜样品的比率, Pb 并且作为比最大值高的集中,在食物(GB2762-2005 ) 的沾染物的层次分别地是 4.7% , 16.3% 和 48.8% 。在蔬菜的重金属的简历集中因素(BCF ) 的顺序是 Cd > Zn > Cu > 作为 > Ni > Hg > Cr > Pb。在 Dongguan 城市里从农业土壤和蔬菜为食物安全和人健康集中于重金属的潜在的风险是必要的,广东省。 | CAI Limei HUANG Lanchun ZHOU Yongzhang XU Zhencheng PENG Xiaochun YAO Ling'ai ZHOU Yang PENG Ping'an | 2010 | Journal of Geographical Sciences2010，20，1： | 23 |
| 2 | Tumor-infiltrating B cells:their role and application in antitumor immunity in lung cancer显示文摘Evidence indicates that lung cancer development is a complex process that involves interactions between tumor cells,stromal fibroblasts,and immune cells.Tumor-infiltrating immune cells play a significant role in the promotion or inhibition of tumor growth.As an integral component of the tumor microenvironment,tumor-infiltrating B lymphocytes(TIBs)exist in all stages of cancer and play important roles in shaping tumor development.Here,we review recent clinical and preclinical studies that outline the role of TIBs in lung cancer development,assess their prognostic significance,and explore the potential benefit of B cell-based immunotherapy for lung cancer treatment. | Si-si Wang Wei Liu Dalam Ly Hao Xu Limei Qu Li Zhang | 2019 | Cellular & Molecular Immunology2019，16，1： | 21 |
| 3 | Human avian influenza A (H5N1) virus infection in China显示文摘Highly pathogenic influenza A (H5N1) virus causes a widespread poultry deaths worldwide. The first human H5N1 infected case was reported in Hong Kong Special Administrative Region of China in 1997. Since then, the virus re-emerged in 2003 and continues to infect people worldwide. Currently, over 400 human infections have been reported in more than 15 countries and mortality rate is greater than 60%. H5N1 viruses still pose a potential pandemic threat in the future because of the continuing global spread and evolution. Here, we summarize the epidemiological, clinical and virological characteristics of human H5N1 infection in China monitored and identified by our national surveillance systems. | XU CuiLin, DONG LiBo, XIN Li, LAN Yu, CHEN YongKun, YANG LiMei & SHU YueLong State Key Laboratory for Viral Genetic and Engineering, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention (China CDC), Beijing 100052, China | 2009 | Science China(Life Sciences)2009，52，5： | 14 |
| 4 | Mechanisms of ultraviolet disinfection and chlorination of Escherichia coli: Culturability, membrane permeability,metabolism, and genetic damage显示文摘Traditional culture methods may underestimate the tolerance of microorganisms to disinfectants because of the existence of viable but nonculturable or sublethally injured cells after disinfection.The selection of a strict method is crucial for the evaluation of disinfection performance.The actions of 2 typical disinfectants–ultraviolet(UV)and chlorine–on the fecal indicator Escherichia coli were investigated by the detection of culturability,membrane permeability,metabolic activity,deoxyribonucleic acid(DNA),and messenger ribonucleic acid(m RNA).During UV disinfection,the irreversible damages in the cell membrane and cellular adenosine triphosphate(ATP)were negligible at low UV doses(<80 m J/cm^2).However,membrane permeability was damaged at low doses of chlorine(<5 mg/L),leading to leakage of cellular ATP.Our study showed that a slight lesion in DNA was detected even at high doses of UV(400 m J/cm^2)and chlorine(>5 mg/L)treatments.The decay of m RNA was more rapid than that of DNA.The degradation level of m RNA depended on the choice of target genes.After exposure to 50 m J/cm^2UV dose or 5 mg/L chlorine for30 min,the DNA damage repair function(Rec A m RNA)was inhibited.The m RNA involved in the DNA damage repair function can be a potential indicator of bacterial viability. | Limei Xu Chongmiao Zhang Pengcheng Xu Xiaochang C.Wang | 2018 | Journal of Environmental Sciences2018，30，3： | 10 |
| 5 | Lyzl4, a novel mouse sperm-related protein, is involved in fertilization显示文摘鸡类型(c 类型) 溶解酵素的角色,原型溶解酵素,在免疫在许多有机体被描绘了。在这研究,我们克隆新奇 c 类型像溶解酵素的基因, Lyzl4,它位于老鼠染色体 9F4 并且与通常认为的信号肽和一个朊酶劈开地点编码了 145 氨基酸。在酵母表示的成熟 recombinant Lyzl4 蛋白质没显示出 bacteriolytic 活动。顺序排列分析证明在 c 类型溶解酵素的 20 不变的残余中的 3 个在 Lyzl4 被改变。改变的氨基酸(D52G ) 之一位于催化领域。Lyzl4 mRNA 有选择地在成年鼠标在睾丸和 epididymis 被表示,与改变越过不同发展阶段的表达式水平。Lyzl4 蛋白质的高水平在 acrosomal 区域和尾巴的主要的片的精子上被发现。在有它的特定的抗体的精子的 Lyzl4 蛋白质的免疫中立化显著地以一种剂量依赖者方式在 vitro 授精百分比减少了,建议 Lyzl4 可能为授精是重要的。 | Ruilin Sun Ruling Shen Jun Li Guojiang Xu Jun Chi Limei Li Jianke Ren Zhugang Wang Jian Fei | 2011 | Acta Biochimica et Biophysica Sinica2011，43，5： | 9 |
| 6 | Safety and effectiveness of Traditional Chinese Medicinal herbs for diabetic foot:a systematic review and Meta-analysis显示文摘OBJECTIVE: To evaluate the safety and effectiveness of traditional Chinese medicinal herbs(TCMHs) as an adjunctive treatment for diabetic foot(DF).METHODS: The sources used were Pub Med(1966 to August 2015), the Cochrane Library(1988 to August 2015), the Excerpta Medica Database(1974 to August 2015), Wiley(1807 to August 2015), Ovid(1988 to August 2015), Clinical Trials.gov(1993 to August 2015), the Cochrane Central Register of Controlled Trials(1966 to August 2015), China Science and Technology Journal Database(1994 to August2015), Chi CTR(2007 to August 2015), Sino Med(1978 to August 2015), the China National Knowledge Infrastructure(1984 to August 2015), Wanfang Data Knowledge Service Platform(1998 to August 2015), and the Traditional Chinese Medical Literature Analysis and Retrieval System(TCMLARS)(1984 to August 2015). Studies were identified and selected, and the data were extracted by two independent reviewers. The Cochrane Risk of Bias tool was used to assess the quality of studies. Revman 5.2 software was used for data synthesis and analysis.RESULTS: Sixteen studies were included based on the selection criteria. Of these, seven studies had low bias risk and one had high bias risk. In the overall analysis, TCMHs resulted in a significantly higher total effective rate(OR 5.08; 95% CI 3.50 to7.36; P < 0.000 01), cure rate(OR 2.12; 95% CI1.63 to 2.77; P < 0.000 01), and shorter time to ulcer healing(SMD-0.64; 95% CI-0.89 to-0.40;P < 0.000 01) when compared with non-TCMHs treated DF. The analysis also revealed that significantly fewer amputations occurred in TCMHs patients(OR 0.36; 95% CI 0.20 to 0.65; P = 0.0007).Sensitivity analysis indicated that the findings of the Meta-analysis were robust to study quality,and the funnel plot of the Egger test showed no publication bias.CONCLUSION: TCMHs intervention appears to be more effective for DF, with a similar safety profile,when compared with non-TCMHs treatments, although this result requires further verification with more well-designed studies. | Chen Shuo Ma Jianwei Xu Limei Niu Tianhui Dong Jing Liu Wenjun Han Qi | 2017 | Journal of Traditional Chinese Medicine2017，37，6： | 5 |
| 7 | Research Progress of Active Ingredients and Pharmacological Action of Honeysuckle显示文摘Honeysuckle,the dry flower bud or with open flower of Lonicera japonica in caprifoliaceae,has been touted as a cure-all for heat-clearing and detoxifying effect.Honeysuckle contains abundant components such as volatile oil,flavonoids and organic acids,playing the role of antibiosis,antivirus,antiphlogosis,immunopotentiation and antioxidation.The active ingredients and pharmacological action of honeysuckle are reviewed in the paper,so as to lay the foundation for further application of honeysuckle in veterinary clinic. | Zhang Zhimei Guo Shijin Fu Shijun Xu Qianqian Yang Limei Zhang Ying Wang Yanping Ma Li Wang Jianjun Shen Zhiqiang | 2014 | Animal Husbandry and Feed Science2014，6，1： | 4 |
| 8 | Effects of Shuanghuanglian Propolis Oral Liquid on Growth Performance,Slaughter Performance and Meat Quality of Broilers显示文摘[Objective] The paper was to investigate the effects of adding Shuanghuanglian propolis oral liquid in drinking water on growth performance,slaughter performance and meat quality of broilers. [Method]Ninety one-day-old broilers were randomly divided into three groups( group Ⅰ,group Ⅱ and group Ⅲ) : group Ⅰ was the control group,and groups Ⅱ and Ⅲ were added 0. 5 and 1. 0 mL / L Shuanghuanglian propolis oral liquid in drinking water,respectively.[Result] Compared with broilers in group I,the average daily feed intakes of broilers in group Ⅱ and group Ⅲ were increased by 0. 86%( P > 0. 05) and 1. 10%( P > 0. 05),respectively; the average daily gains were increased by 2. 33%( P < 0. 05) and 4. 43%( P < 0. 05),respectively; the feed gain ratios were decreased by 1. 05%( P >0. 05) and 2. 63%( P <0. 05),respectively; the slaughter rates were increased by 0. 94%( P >0. 05) and 1. 70%( P <0. 05),respectively; the eviscerated rates were increased by 0. 52%( P > 0. 05) and 1. 15%( P > 0. 05),respectively; the semi-eviscerated rates were increased by 1. 51%( P < 0. 05) and 2.23%( P < 0. 05),respectively; the breast muscle rates were increased by 3. 77%( P < 0. 05) and 5. 51%( P < 0. 05),respectively; the leg muscle rates were increased by 1. 96%( P > 0. 05) and 4. 09%( P < 0. 05),respectively; the lightness( L*) were decreased by 0. 96%( P > 0. 05) and 1. 47%( P > 0. 05),respectively; the redness( a*) were increased by 1. 24%( P < 0. 05) and 1. 86%( P < 0. 05),respectively; the yellowness( b*) were decreased by 0. 44%( P >0. 05) and 1. 03%( P <0. 05),respectively; the drip losses were decreased by 1. 22%( P <0. 05) and 1. 56%( P <0. 05),respectively; the cooking losses were decreased by 1. 76%( P > 0. 05) and 2. 89%( P < 0. 05),respectively. [Conclusion]Adding 0. 5 and 1. 0 mL / L Shuanghuanglian propolis oral liquid in drinking water could improve growth performance,slaughter performance,meat quality of broilers,and the dose of 1. 0 mL / L is recommended in clinical application. | Ma Li Yang Limei Shen Zhiqiang Wang Yanping Xu Qianqian Zhang Ying Guo Shijin | 2016 | Animal Husbandry and Feed Science2016，8，4： | 4 |
| 9 | MS1 is essential for male fertility by regulating the microsporocyte cell plate expansion in soybean显示文摘Male sterility is an essential trait in hybrid seed production,especially for monoclinous and autogamous food crops.Soybean male-sterile ms1 mutant has been known for more than 50 years and could be instrumental in making hybrid seeds.However,the gene responsible for the male-sterile phenotype has remained unknown.Here,we report the map-based cloning and characterization of the MS1 gene in soybean.MS1 encodes a kinesin protein and localizes to the nucleus,where it is required for the male meiotic cytokinesis after telophase Ⅱ.We further substantiated that MS1 colocalizes with microtubules and is essential for cell plate formation in soybean male gametogenesis through immunostaining.Both ms1 and CRISPR/Cas9 knockout mutants show complete male sterility but are otherwise phenotypically normal,making them perfect tools for producing hybrid seeds.The identification of MS1 has the practical potential for assembling the sterility system and speeding up hybrid soybean breeding. | Xiaolong Fang Xiaoyuan Sun Xiangdong Yang Qing Li Chunjing Lin Jie Xu Wenjun Gong Yifan Wang Lu Liu Limei Zhao Baohui Liu Jun Qin Mengchen Zhang Chunbao Zhang Fanjiang Kong Meina Li | 2021 | Science China(Life Sciences)2021，64，9： | 4 |
| 10 | Occurrence of sulfonamide and tetracycline-resistant bacteria and resistance genes in aquaculture environment显示文摘 | Panpan Gao Daqing Mao Yi Luo Limei Wang Bingjie Xu Lin Xu | 2012 | Water Research2012，，7： | 3 |
| 11 | The microbial metallogeny of weathering crust REE deposits in South China显示文摘VERY little is known about the roles of microorganisms in the metallogeny of weathering crust REE deposits, although there has been much literature on REE behavior in weathering conditions. The present note deals with the microorganisms in 4 weathering profiles of an REE deposit and their roles in migration and accumulation of REE in weathering conditions. 1 Cultivation and identification of microorganisms Under strict sterilization, samples were collected from various parts of 4 weathering profiles of granites in a weathering crust REE deposit in South China. The samples were then cultivated for microorganisms with ordinary agars as culture media and then the cultivated microorganisms were dyed and identifiedunder a microscope. The sampled positions and cultivated numbers of microbial colonies are shown | CHEN Binghui~(1,2), WANG Zhimei~3, HUANG Limei~3, WU Fuhai~3, CHEN Jingde~2 and XU Wenlie~2 1. Department of Earth Sciences, Nanjing University, Nanjing 210093, China 2. Department of Earth Sciences, Zhongshan University, Guangzhou 510275, China 3. Guangdong Pharmaceutical College, Guangzhou 510244, China | 1999 | Chinese Science Bulletin1999，44，S2： | 3 |
| 12 | The RALF1-FERONIA complex interacts with and activates TOR signaling in response to low nutrients显示文摘Target of rapamycin(TOR)kinase is an evolutionarily conserved major regulator of nutrient metabolism and organismal growth in eukaryotes.In plants,nutrients are remobilized and reallocated between shoots and roots under low-nutrient conditions,and nitrogen and nitrogen-related nutrients(e.g.,amino acids)are key upstream signals leading to TOR activation in shoots under low-nutrient conditions.However,how these forms of nitrogen can be sensed to activate TOR in plants is still poorly understood.Here we report that the Arabidopsis receptor kinase FERON IA(FER)interacts with the TOR pathway to regulate nutrient(nitrogen and amino acid)signaling under low-nutrient conditions and exerts similar metabolic effects in response to nitrogen deficiency.We found that FER and its partner,RPM1-induced protein kinase(RIPK),interact with the TOR/RAPTOR complex to positively modulate TOR signaling activity.During this process,the receptor complex FER/RIPK phosphorylates the TOR complex component RAPTOR1B.The RALF1 peptide,a ligand of the FER/RIPK receptor complex,increases TOR activation in the young leaf by enhancing FER-TOR interactions,leading to promotion of true leaf growth in Arabidopsis under lownutrient conditions.Furthermore,we showed that specific amino acids(e.g.,Gin,Asp,and Gly)promote true leaf growth under nitrogen-deficient conditions via the FER-TOR axis.Collectively,our study reveals a mechanism by which the RALF1-FER pathway activates TOR in the plant adaptive response to low nutrients and suggests that plants prioritize nutritional stress response over RALF1-mediated inhibition of cell growth under low-nutrient conditions. | Limei Song Guoyun Xu Tingting Li Huina Zhou Qinlu Lin Jia Chen Long Wang Dousheng Wu Xiaoxu Li Lifeng Wang Sirui Zhu Feng Yu | 2022 | Molecular Plant2022，15，7： | 2 |
| 13 | Expression and Characterization of Recombinant Thermostable Alkaline Phosphatase from a Novel Thermophilic Bacterium Thermus thermophilus XM显示文摘从 thermophilic 细菌 Thermus thermophilus XM 编码热水瓶桌子碱性磷酸酶的基因(龙头) 被克隆并且定序。它长是 1506 bp 并且与推出的氨基酸顺序的 54.7 kDa.Comparison 的一个计算的分子的团编码 501 氨基酸残余的蛋白质,另外的碱性磷酸酶出现了在 phosphorylation 地点和金属结合部位的附近的区域是,这高度保存了。recombinant 热水瓶桌子碱性磷酸酶在 Escherichia 关口 i 被表示为标注 His6 的熔化蛋白质,它的酶的性质在纯化以后被描绘。为 recombinant 热水瓶桌子碱性磷酸酶活动的 pH 和温度 optima 是 12 和 75 °C .As 期望的 pH ,酶显示了高 thermostability ,在在 80 °C .Its 为 6 h 孵化以后保留超过50%活动催化功能面对 0.1 公里被加速Co^(2+),Fe^(2+),Mg^(2+),或Mn^(2+)但是被 2.0 公里Fe^(2+)强烈禁止。在最佳的条件下面, Michaeli 为 p-nitrophenyl-phosphate 的劈开的常数(K_m ) 是它就和另外的碱性磷酸酶一样有的 0.034 mM.Although, recombinant 热水瓶桌子碱性磷酸酶拥有一些唯一的特征,例如高最佳的 pH 和好 thermostability。 | Jianbo LI Limei XU Feng YANG | 2007 | Acta Biochimica et Biophysica Sinica2007，39，11： | 2 |
| 14 | Mn atomic layers under inert covers of graphene and hexagonal boron nitride prepared on Rh(111)显示文摘进 graphene 和它的支持的底层的接口的金属原子的置闰为削弱联合并且构造金属的接口的缘故成为了一个吸引人的话题在惰性的封面下面的原子层。然而,这新奇行为很少在类似的六角形的硼氮化物(h-BN ) 上被报导了在金属底层上综合了。这里,我们描述 Mn 置闰的比较研究进 graphene/Rh (111 ) 和 h-BN/Rh (111 ) 的接口,由使用解决 atomically 的扫描通道显微镜学(STM ) 和密度功能的理论(DFT ) 计算。置闰被退火执行同样扔的 Mn 簇,和进 h-BN/Rh (111 ) 的 Mn 置闰的开始的温度被发现是 80 瑩 ?? 数歡瀠睯牥搠湥楳祴漠 ??? ″坭洯吗? | Yu Zhang Yanfeng Zhang Donglin Ma Qingqing Ji Wei Fang Jianping Shi Teng Gao Mengxi Liu Yabo Gao Yubin Chen Limei Xu Zhongfan Liu | 2013 | Nano Research2013，6，12： | 2 |
| 15 | Factors influencing bioleaching copper from waste printed circuit boards by Acidithiobacillus ferrooxidans显示文摘 | Tao Yang Zheng Xu Jiankang Wen Limei Yang | 2008 | Hydrometallurgy2008，，1： | 2 |
| 16 | Characterization of a Novel Weak Allele of RGA1/D1 and Its Potential Application in Rice Breeding显示文摘Semi-dwarfing improves the lodging resistance and yield of rice,and the vast majority of modern rice varieties harbor the sd1 allele to decrease plant height,resulting in reduced genetic diversity and negative agronomic traits.Thus,exploring alternative sources of dwarfism is imperative for rice breeding.Here,we identified a novel RGA1 allele,d1-w,from a local indica variety Xiaolixiang(XLX)using a map-based cloning approach.Compared with other rice varieties,RGA1 in XLX contained a unique single nucleotide polymorphism that resulted in an additional transcript and reduced functional RGA1 transcript level.The RGA1 from Nipponbare was introduced into XLX to estimate the value of d1-w in rice breeding.Compared with transgenic XLX plants(XLX^(D1)),XLX exhibited reduced plant height,increased stem strength,lower reactive oxygen species accumulation,delayed senescence,stronger photosynthesis,higher grain yield and quality(including external,milling and nutritional qualities),and enhanced resistance to drought and Rhizoctonia solani.Therefore,we proposed that the d1-w allele has potential as an excellent dwarfism resource for rice breeding. | LIU Yantong LI Ting JIANG Zhishu ZENG Chuihai HE Rong QIU Jiao LIN Xiaoli PENG Limei SONG Yongping ZHOU Dahu CAI Yicong ZHU Changlan FU Junru HE Haohua XU Jie | 2022 | Rice science2022，29，6： | 2 |
| 17 | Molecular modulating of cobalt phthalocyanines on aminofunctionalized carbon nanotubes for enhanced electrocatalytic CO_(2)conversion显示文摘Metal porphyrins and metal phthalocyanines(Pc)constitute a promising class of metal molecular catalysts(MMCs)for efficient CO_(2)-to-CO electrocatalytic conversion due to their well-defined molecular structures.How to adjust the local coordination and electronic environment of the metal center and enhance the molecular-level dispersion of the active components remains as great challenges for further improving the performance.Herein,a cobalt(II)Pc(CoPc)-COOH/carbon nanotube(CNT)-NH_(2)hybrid catalyst was rationally designed by clicking the CoPc-COOH molecules onto the surface of CNT-NH_(2)through amidation reaction.This novel hybrid catalyst exhibited the enhanced current density of 22.4 mA/cm2 and CO selectivity of 91%at−0.88 V vs.reversible hydrogen electrode(RHE)in the CO_(2)electroreduction,as compared with CoPc-COOH/CNT and CoPc/CNT samples.The superior activity was ascribed to the charge transfer induced by introduction of-COOH and-NH_(2)functional groups to CoPc and CNT,respectively,facilitating the active centers of CoI being generated at lower potentials,and leading to the highest turnover frequency(TOF)being obtained over the CoPc-COOH/CNT-NH_(2)hybrid catalyst.The inherent directivity and saturability of covalent bonds formed via the amidation reaction ensure not only a higher density of Co active centers,but also an improved stability for CO_(2)reduction reaction(CO_(2)RR).The present study represents an effective strategy for improving MMCs performance by molecular modulating of metal phthalocyanines on functionalized carbon substrates directed by click confinement chemistry. | Huajian Xu Huizhu Cai Linxia Cui Limei Yu Rui Gao Chuan Shi | 2023 | Nano Research2023，16，3： | 2 |
| 18 | Expression,purification and characterization of Mycobacterium tuberculosis RpfE protein显示文摘Resuscitation promoting factor E(RpfE)is one of the five Rpf-like proteins in Mycobacterium tuberculosis(M.tuberculosis).These Rpf-like proteins are secretory,which make them candidates for recognition by the host immune system.In this study,the RpfE gene was amplified from M.tuberculosis,cloned into the expression vectors pDE22 and pPRO EXHT,and were expressed in Mycobacterium vaccae(M.vaccae)and Escherichia coli DH5α,respectively.Both recombinant RpfE proteins were purified by Ni-Sepharose affinity chromatography,and were given to C57BL/6 mice.The RpfE proteins elicited T cell proliferation,and stimulated the production of gamma interferon(IFN-γ),interleukin-10(IL-10)and IL-12.Our results indicated that the RpfE protein expressed in M.vaccae could more efficiently stimulate cellular immune response,making it a promising candidate as a subunit vaccine. | Ying Xue Yinlan Bai Xue Gao Hong Jiang Limei Wang Hui Gao Zhikai Xu | 2012 | The Journal of Biomedical Research2012，26，1： | 2 |
| 19 | Citron C-05 inhibits both the penetration and colonization of Xanthomonas citri subsp.citri to achieve resistance to citrus canker disease显示文摘Citrus canker,caused by Xanthomonas citri subsp.citri(Xcc),is a serious bacterial disease that affects citrus production worldwide.Citron C-05(Citrus medica)is the only germplasm in the Citrus genus that has been identified to exhibit strong resistance to Xcc.However,it has not been determined when,where,and how Xcc is restricted in the tissues of Citron C-05 during the infection process.In the present study,we investigated the spatiotemporal growth dynamics of an eGFP-labeled virulent Xcc(eGFP-Xcc)strain in Citron C-05 along with five susceptible biotypes(i.e.,lemon,pummelo,sour orange,sweet orange,and ponkan mandarin)upon inoculation via the spraying or leaf infiltration of a bacterial suspension.The results from extensive confocal laser scanning microscopy analyses showed that while Xcc grew rapidly in plants of all five susceptible genotypes,Xcc was severely restricted in the epidermal and mesophyll cell layers of the leaves of Citron C-05 in the early stage of infection.Not surprisingly,resistance against Xcc in Citron C-05 was found to be associated with the production of reactive oxygen species and hypersensitive response-like cell death,as well as greater upregulation of several defense-related genes,including a pathogenesis-related gene(PR1)and a glutathione S-transferase gene(GST1),compared with sweet orange as a susceptible control.Taken together,our results not only provide further valuable details of the spatiotemporal dynamics of the host entry,propagation,and spread of Xcc in both resistant and susceptible citrus plants but also suggest that resistance to Xcc in Citron C-05 may be attributed to the activation of multiple defense mechanisms. | Hongyan Fu Mingming Zhao Jing Xu Limei Tan Jian Han Dazhi Li Meijun Wang Shunyuan Xiao Xianfeng Ma Ziniu Deng | 2020 | Horticulture Research2020，7，1： | 2 |
| 20 | Spatial transcriptome analysis of long non-coding RNAs reveals tissue specificity and functional roles in cancer显示文摘Long non-coding RNAs(lncRNAs)play a significant role in maintaining tissue morphology and functions,and their precise regulatory effectiveness is closely related to expression patterns.However,the spatial expression patterns of lncRNAs in humans are poorly characterized.Here,we constructed five comprehensive transcriptomic atlases of human lncRNAs covering thousands of major tissue samples in normal and disease states.The lncRNA transcriptomes exhibited high consistency within the same tissues across resources,and even higher complexity in specialized tissues.Tissue-elevated(TE)lncRNAs were identified in each resource and robust TE lncRNAs were refined by integrative analysis.We detected 1 to 4684 robust TE lncRNAs across tissues;the highest number was in testis tissue,followed by brain tissue.Functional analyses of TE lncRNAs indicated important roles in corresponding tissue-related pathways.Moreover,we found that the expression features of robust TE lncRNAs made them be effective biomarkers to distinguish tissues;TE lncRNAs also tended to be associated with cancer,and exhibited differential expression or were correlated with patient survival.In summary,spatial classification of lncRNAs is the starting point for elucidating the function of lncRNAs in both maintenance of tissue morphology and progress of tissue-constricted diseases. | Kang XU Xiyun JIN Ya LUO Haozhe ZOU Dezhong LV Liping WANG Limei FU Yangyang CAI Tingting SHAO Yongsheng LI Juan XU | 2023 | Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)2023，24，1： | 1 |
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