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| 1 | A Novel Hydrolytic Product from Flesh of Mactra veneriformis and Its Bioactivities in Calcium Supplement显示文摘To prepare calcium-binding peptides, the flesh residue of Mactra Veneriformis was subjected to enzymatic hydrolysis. By comparing the capability of combining calcium of the hydrolyzates, pepsin was confirmed to be the most suitable enzyme for hydrolyzing the flesh residue to release calcium-binding peptides among the seven tested proteases. The pepsin hydrolyzate (PHM) was divided into three fractions according to the molecule weight of its composition, which ranged from 0.5 to 15 kDa. The low-molecule-weight fraction named PHM-3 had the highest capability in combining calcium. The peptides existing in the PHM-3 fraction consisted of higher contents of Glu, Ala and Leu, and could produce one type of calcium-peptide complex by powerfully chelating calcium ions. PHM-3 products could effectively increase calcium absorption and retention while they decreased the calcium excretion in animal tests. Additionally, symptoms caused by low calcium bioavailability in ovariectomized rats, such as bone mineral density reduction and mechanical strength loss could be significantly ameliorated by the hydrolytic products addition in diet. | WANG Lingchong CHEN Shiyong LIU Rui WU Hao | 2012 | Journal of Ocean University of China2012，11，3： | 4 |
| 2 | Kinematics Analysis and Optimization of the Fast Shearing-extrusion Joining Mechanism for Solid-state Metal显示文摘Dynamical Joining of the solid-state metal is the key technology to realize endless hot rolling. The heating and laser welding method both require long joining time. Based on super deformation method, a 7-bar and 2-slider mechanism was developed in Japan, and the joining time is less than 0.5 s, however the length of each bar are not reported and this mechanism is complex. A relatively simple 6-bar and 1-slider mechanism is put forward, which can realize the shearing and extrusion motion of the top and bottom blades with a speed approximately equal to the speed of the metal plates. In order to study the kinematics property of the double blades, based on complex vector method, the multi-rigid-body model is built, and the displacement and speed functions of the double blades, the joining time and joining thickness are deduced, the kinematics analysis shows that the initial parameters can't satisfy the joining process. Hence, optimization of this mechanism is employed using genetic algorithm(GA) and the optimization parameters of this mechanism are obtained, the kinematics analysis show that the joining time is less than 0.1 s, the joining thickness is more than 80% of the thickness of the solid-state metal, and the horizontal speeds of the blades are improved. A new mechanism is provided for the joining of the solid-state metal and a foundation is laid for the design of the device. | ZHANG Shuangjie YAO Yunfeng LI Lingchong WANG Lijuan LI Junxia LI Qiang | 2015 | Chinese Journal of Mechanical Engineering2015，28，6： | 3 |
| 3 | Hyperglycemia not hypoglycemia alters neuronal dendrites and impairs spatial memory显示文摘 | John IMalone SuzanHanna SamuelSaporta Ronald FMervis Collin RPark LingChong David MDiamond | 2008 | Pediatric Diabetes2008，，6： | 1 |
| 4 | Hyperglycemia not hypoglycemia alters neuronal dendrites and impairs spatial memory显示文摘 | John IMalone SuzanHanna SamuelSaporta Ronald FMervis Collin RPark LingChong David MDiamond | 2008 | Pediatric Diabetes2008，，6： | 1 |
| 5 | Characterisation of nucleosides and nuclebases in Mactra veneriformis by high performance liquid chromatography coupled with diode array detector-mass spectrometry( HPLC-DAD-MS )显示文摘 | Rui Liu Jing Ji Lingchong Wang | 2012 | Food Chemistry2012，135，： | 1 |
| 6 | Isolation and structuralelucidation of novel homogenous polysaccharide from Mactraveneriformis显示文摘 | WANG Lingchong ZHANG Kun DI Liuqing | 2010 | Carbohydrate Polymers2010，86，2： | 1 |
| 7 | Antioxidant activitiesand antioxidative components in the surf clam, Mactra veneriformis显示文摘 | LUAN Hemi WANG Lingchong WU Hao | 2011 | Natural Product Research2011，25，1920： | 1 |
| 8 | β-FeOOH Modified Sludge Biochar as Efficient Adsorbent for Phosphate Removal显示文摘β⁃FeOOH modified sludge biochar(FSBC)was prepared and investigated in this work to remove phosphate from water via adsorption.FSBC exhibited superior adsorption performance for phosphate with the maximum adsorption amount of 27.17 mg/g at 25℃.The specific adsorption capacity of FSBC was 57.8%higher than that of commercial activated carbon at pH 5 with the initial phosphate concentration of 10 mg/L.Coexisting anions can inhibit the adsorption of phosphate,but cations(Cr(VI)and Cd^(2+))exhibited some promotion effect.The results of kinetic and isotherm models and characterization analysis in combination indicated chemical adsorption,as electrostatic interaction,metal phosphate precipitation and hydrogen bonding may be the dominant process.The adsorption of phosphate onto FSBC was a monolayer adsorption,and the process was endothermic.After four adsorption/desorption cycles,the adsorption capacity of FSBC for phosphate decreased by 36.4%,which was in a distinct comparison with the 57.5%of SBC. | Yanping Zhang Jiahua Pei Fen Li Lingchong Li Jiayu Tian | 2022 | Journal of Harbin Institute of Technology(New Series)2022，29，3： | 1 |
| 9 | Hydrolysate of corn starch saccharified with a dual-enzyme system and the effect on the crystallization of itaconic acid when used in fermentation显示文摘An improved process of hydrolysis of corn starch was adopted in the production of itaconic acid(IA),the aim was to decrease the unfermentable reducing sugar (RS) in the medium from the beginningof the fermentation and to increase the crystallization efficiency of IA from the fermentation broth.Theglucose (GS) syrups saccharified by several combinations of glucoamylase and pullulanase were investi-gated and used as the carbohydrate source of the fermentation medium for the spore-initiated submergedfermentations experiments.Compared with the conventional process (with ptdlulanase controlled),theimproved process decreased the RS residue in the fermentation broth from 3.01g/L to 1.35g/L and from4.25g/L to 3.25g/L when the original RS of the medium were 100 and 120g/L,respectively.The crys-tallization efficiency of IA increased from 65 % to 78.8 % and from 69.58 % to 82.81% with the originalRS being 100 and 120g/L,respectively. | 李丕武 Chen Xiguang Liu Jianjun Wang Lingchong | 2008 | High Technology Letters2008，14，3： | 0 |
| 10 | Quantifying E2F1 protein dynamics in single cells显示文摘Background:E2F1 protein,a major effector of the Rb/E2F pathway plays a central role in regulating cell-fate decisions involved in proliferation,apoptosis,and differentiation.Its expression is highly dynamic and tightly modulated through a combination of transcriptional,translational and posttranslational controls.However,the mechanisms by which its expression and activity can promote different cellular outcomes remain to be fully elucidated.To better document E2F1 expression in live cells,we have engineered a series of fluorescent E2F1 protein reporters that quantitatively capture E2F1 protein dynamics.Methods:Reporter constructs,under the control of the mouse or human E2F1 proximal promoter,were designed to express an E2F1-Venus fusion protein incapable of binding DNA.In addition,constructs either included or excluded the y untranslated region(3fUTR)of the E2F1 gene.These constructs were introduced into fibroblasts and epithelial cells,and expression of the fusion reporter protein was validated and quantified in single cells using live imaging.Results:In all cases,expression of the reporter protein effectively recapitulated the behavior of E2F1 under various conditions,including cell cycle progression and genotoxic stress.No or little fluorescent signal of the reporter was detected in G〇,but as the cycle progressed,expression of the reporter protein steadily increased in the nucleus,peaking a few hours before cell division,but declining to baseline 2-3 h prior to the onset of mitosis.The absence of the E2F13 fUTR in the constructs led to considerably higher steady-state levels of the fusion protein,which although normally regulated,exhibited a slightly less complex dynamic profile during the cell cycle or genotoxic stress.Lastly,the presence or absence of Rb failed to impact the overall detection and levels of the reporter proteins.Conclusions:Our validated E2F1 protein reporters complement nicely other reporters of the Rb/E2F pathway and provide a unique tool to follow the complex dynamics of E2F1 expression in real time in single cells. | Bernard Mathey-Prevot Bao-Tran Parker Carolyn Im Cierra Hong Peng Dong Guang Yao Lingchong You | 2020 | Quantitative Biology2020，8，1： | 0 |
| 11 | Construction, visualization, and analysis of aiological network models in Dynetica显示文摘 | Derek Eidum Kanishk Asthana Samir Unni Michael Deng Lingchong You | 2014 | Frontiers of Electrical and Electronic Engineering in China2014，9，4： | 0 |
| 12 | Predictive power of cell-to-cell variability显示文摘 | Bochong Li Lingchong You | 2013 | Frontiers of Electrical and Electronic Engineering in China2013，8，2： | 0 |
| 13 | Hierarchical ultrastructure:An overview of what is known about tendons and future perspective for tendon engineering显示文摘Abnormal tendons are rarely ever repaired to the natural structure and morphology of normal tendons.To better guide the repair and regeneration of injured tendons through a tissue engineering method,it is necessary to have insights into the internal morphology,organization,and composition of natural tendons.This review summarized recent researches on the structure and function of the extracellular matrix(ECM)components of tendons and highlight the application of multiple detection methodologies concerning the structure of ECMs.In addition,we look forward to the future of multi-dimensional biomaterial design methods and the potential of structural repair for tendon ECM components.In addition,focus is placed on the macro to micro detection methods for tendons,and current techniques for evaluating the extracellular matrix of tendons at the micro level are introduced in detail.Finally,emphasis is given to future extracellular matrix detection methods,as well as to how future efforts could concentrate on fabricating the biomimetic tendons. | Shichen Zhang Wei Ju Xiaoyi Chen Yanyan Zhao Lingchong Feng Zi Yin Xiao Chen | 2022 | Bioactive Materials2022，7，2： | 0 |
| 14 | Anti-hyperglycemic effect of the polysaccharide fraction isolated from mactra veneriformis显示文摘Total macromolecule extract was obtained from the soft body of Mactra veneriformis by the coupling techniques of decoction and alcohol precipitation.The extract was deproteinized with an ion exchange column,and resulted in the purifying of the crude polysaccharide fraction.It was found by chemical analysis that the crude polysaccharide part is composed of abundant polysacchar-ides(>95%)and few proteins(<1%).Furthermore,only one type of monosaccharide,glucose,was detected from its hydrolytes by thin-layer chromatography,indicating that the polysaccharides might be analogs of glucosan.The anti-hyperglycemia effects of the crude polysaccharide part were preliminarily investigated using several pharmacolo-gical methods in normal and diabetic mice.Animal experimental results showed that the crude polysaccharide fraction exhibited proper glycemia inhibition activity,and 300 mg/kg-weight dose has the optimal effect among all the studied doses.It is concluded that the crude polysaccharide fraction can be explored as a novel health product that possesses potential as an anti-hyperglycemic agent. | Lingchong WANG Hao WU Nian CHANG Kun ZHANG | 2011 | Frontiers of Chemical Science and Engineering2011，5，2： | 0 |
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