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| 1 | Human natural killer cells for targeting delivery of gold nanostars and bimodal imaging directed photothermal/photodynamic therapy and immunotherapy显示文摘Objective:To construct a novel nanoplatform GNS@CaCO3/Ce6-NK by loading the CaCO3-coated gold nanostars(GNSs)with Chlorin e6 molecules(Ce6)into human peripheral blood mononuclear cells(PBMCs)-derived NK cells for tumor targeted therapy.Methods:GNS@CaCO3/Ce6 nanoparticles were prepared and characterized by TEM and UV-vis.The cell surface markers and cytokines secretion of NK cells before and after loading the GNS@CaCO3/Ce6 nanoparticles were detected by Flow Cytometry(FCM)and ELISA.Effects of the GNS@CaCO3/Ce6-NK cells on A549 cancer cells was determined by FCM and CCK-8.Intracellular fluorescent signals of GNS@CaCO3/Ce6-NK cells were detected via Confocal laser scanning microscopic(CLSM)and FCM at different time points.Intracellular ROS generation of GNS@CaCO3/Ce6-NK cells under laser irradiation were examined by FCM.The distribution of GNS@CaCO3/Ce6-NK in A549 tumor-bearing mice were observed by fluorescence imaging and PA imaging.The combination therapy of GNS@CaCO3/Ce6-NK under laser irradiation were investigated on tumor-bearing mice.Results:The coated CaC03 shell on the surface of GNSs exhibited prominent delivery and protection effect of Ce6 during the cellular uptake process.The as-prepared multifunctional GNS@CaCO3/Ce6-NK cells possessed bimodal functions of fluorescence imaging and photoacoustic imaging.The as-prepared multifunctional GNS@CaCO3/Ce6-NK cells could actively target tumor tissues with the enhanced photothermal/photodynamic therapy and immunotherapy.Conclusions:The GNS@CaCO3/Ce6-NK shows effective tumor-targeting ability and prominent therapeutic efficacy toward lung cancer A549 tumor-bearing mice.Through fully utilizing the features of GNSs and NK cells,this new nanoplatform provides a new synergistic strategy for enhanced photothermal/photodynamic therapy and immunotherapy in the field of anticancer development in the near future. | Bin Liu Wen Cao Jin Cheng Sisi Fan Shaojun Pan Lirui Wang Jiaqi Niu Yunxiang Pan Yanlei Liu Xiyang Sun Lijun Ma Jie Song Jian Ni Daxiang Cui | 2019 | Cancer Biology & Medicine2019，16，4： | 7 |
| 2 | ADSORPTION OF LDL ON THE MODIFIED CHITOSAN显示文摘In this paper, the selective adsorption of LDL on chitosan modified with PEG and Asp. was studied. The adsorption rate of LDL and HDL on the double modified chitosan was 57% and 12% respectively. The results shown that the double modified chitosan can be used a adsorbent for selective binding to LDL. This work may help to develop functional columns for hemoperfusion. | LIU Manying ZHAO Lirui TAI Liwen JI Xueping YANG Chunxia WANG Aiping | 2000 | Chinese Journal of Reactive Polymers2000，9，2： | 3 |
| 3 | Crucial control measures to contain China's first Delta variant outbreak显示文摘The SARS-C oV-2 B.1.617.2(Delta) variant flared up in late May in Guangzhou,China.Transmission characteristics of Delta variant were analysed for 153 confirmed cases and two complete transmission chains with seven generations were fully presented.A rapid transmission occurred in five generations within10 days.The basic reproduction number(R_0) was 3.60(95% confidence interval:2.50-5.30).After redefining the concept of close contact,the proportion of confirmed cases discovered from close contacts increased from 43% to 100%.With the usage of a yellow health code,the potential exposed individuals were self-motivated to take a nucleic acid test and regained public access with a negative testing result.Facing the massive requirement of screening,novel facilities like makeshift inflatable laboratories were promptly set up as a vital supplement and 17 cases were found,with 1 pre-symptomatic.The dynamic adjustment of these three interventions resulted in the decline of Rt from 5.00 to 1.00 within 9 days.By breaking the transmission chain and eliminating the transmission source through extending the scope of the close-contact tracing,health-code usage and mass testing,the Guangzhou D elta epidemic was effectively contained. | Lei Luo Zifeng Yang Jingyi Liang Yu Ma Hui Wang Chitin Hon Mei Jiang Zhengshi Lin Wenda Guan Zhitong Mai Yongming Li Kailin Mai Zhiqi Zeng Chuanmeizi Tu Jian Song Bin Liu Yong Liu Jianfeng He Huiyuan Li Bosheng Li Hang Dong Yutian Miao Shujun Fan Lirui Fan Xingyi Liang Ke Li Chun Chen Huihong Deng Zhicong Yang Nanshan Zhong | 2022 | National Science Review2022，9，4： | 2 |
| 4 | Comparative transcriptome analysis reveals differential gene expression in resistant and susceptible tobacco cultivars in response to infection by cucumber mosaic virus显示文摘Cucumber mosaic virus(CMV) is one of the most severe viral diseases transmitted by aphids infecting Solanum crops in China, causing great losses of crop yields and income in rural communities.The tobacco cultivars NC82 and Taiyan 8 are closely related but differ in resistance to CMV.NC82 is susceptible to infection and Taiyan 8 is resistant, but the mechanisms underlying this difference in resistance are not clear.In this study, we conducted RNA sequencing to analyze changes in gene expression induced in the leaves of Taiyan 8 and NC82 upon systemic infection with CMV, compared with gene expression in the leaves of mock-inoculated plants.Leaves were sampled at one, three, eight, and 15 days after infection.In total, 3443 and 747 differentially expressed genes were identified in Taiyan 8 and NC82, respectively.Gene ontology and pathway enrichment analyses revealed that the different responses to CMV infection between cultivars were based on microtubulebased processes, pentose and glucuronate interconversions, plant–pathogen interaction,and hormone signal transduction pathways.Genes encoding pathogenesis-related proteins, disease-resistance proteins, lipoxygenase, cellulose synthase, an auxin response factor, and an ethylene receptor showed different expression patterns.The differences in gene expression following CMV infection likely contributed to the different resistance levels of these two tobacco cultivars.The comprehensive transcriptome dataset described here,which includes candidate response genes, will serve as a resource for further studies of the molecular mechanisms associated with tobacco defense responses against CMV. | Dan Liu Yazeng Cheng Min Gong Qiang Zhao Caihong Jiang Lirui Cheng Min Ren Yuanying Wang Aiguo Yang | 2019 | The Crop Journal2019，7，3： | 2 |
| 5 | Effects of Different Dietary Energy and Protein Levels on Meat Performance and Meat Quality of Jinghai Yellow Chickens显示文摘To investigate the effect of different dietary energy and protein levels on meat performance and meat quality of Jinghai yellow chickens,480 43-day old Jinghai yellow chickens with similar weight were randomly divided into four experimental groups: experimental group 1( protein 15%,metabolic energy 9. 95 MJ /kg),experimental group 2( protein 16%,metabolic energy 10. 95 MJ /kg),experimental group 3( protein 17%,metabolic energy 12. 65 MJ /kg) and experimental group 4( protein 18%,metabolic energy 13. 95 MJ /kg),respectively. All chickens were slaughtered at 112-day old. The breast and leg muscles of Jinghai yellow chickens were collected,to determine the slaughter performance,conventional meat quality and muscle chemical indicators. The results indicated that dressing-out percentage and eviscerated yield percentage in four experimental groups were above 87. 27% and 67. 00%,respectively; other slaughter performance indicators exhibited no significant differences among various groups( P > 0. 05); breast muscle color of hens in experimental group 4 varied significantly from that in other three groups( P < 0. 05); leg muscle color of hens in experimental group 2 varied extremely significantly from that in other three groups( P < 0. 01); water-holding capacity of breast muscles of hens in experimental group 3 was significantly higher than that in experimental group 4( P < 0. 05); thiamine content of breast muscles of cocks in experimental group 3 was significantly higher than that in experimental group 2( P < 0. 05); however,other properties exhibited no significant differences among various groups( P > 0. 05). | Jianfeng WANG Lulu CUI Kaizhou XIE Yingying SUN Lirui SUN Jianyu LIU Genxi ZHANG Guojun DAI Jinyu WANG | 2014 | Agricultural Biotechnology2014，3，4： | 2 |
| 6 | Deficiency of intestinal mucin‐2 ameliorates experimental alcoholic liver disease in mice显示文摘 | Phillipp Hartmann Peng Chen Hui J. Wang Lirui Wang Declan F. McCole Katharina Brandl Peter St?rkel Clara Belzer Claus Hellerbrand Hidekazu Tsukamoto Samuel B. Ho Bernd Schnabl | 2013 | Hepatology2013，，： | 1 |
| 7 | Methods to determine intestinal permeability and bacterial translocation during liver disease显示文摘 | Lirui Wang Cristina Llorente Phillipp Hartmann An-Ming Yang Peng Chen Bernd Schnabl | 2015 | Journal of Immunological Methods2015，，： | 1 |
| 8 | Long-term high-resolution invivo imaging of cerebral cortical structures following ischemic stroke显示文摘The development of multi-photon microscopic technique has made it possible to image submicron structures deep in biological tissues.This technique is widely used for imaging of cortical structures in developing and adult animals,and there have been detail descriptions of in vivo imaging of synaptic structures in normal animals through a thinned-skull or open-skull cranial window.However,several challenges should be considered carefully for high-resolution imaging of cortical structures under pathological conditions.Here we describe a protocol for in vivo imaging of dendritic structures following ischemic stroke through thinned skull.This protocol can also be applied for acute or chronic imaging of neuronal structural plasticity,glial activation,cerebral microcirculation,or synaptic functions in other pathological conditions. | Lei Wang Lirui Zhu Junru Liu Shengxiang Zhang | 2020 | Biophysics Reports2020，6，4： | 0 |
| 9 | Analysis on How to Improve Competency of Student-affairs-administrators in Colleges and Universities显示文摘Facing new situations and development,ideological and political work in colleges and universities presents new characteristics under the new circumstances.,which requires student-affairsadministrators to have a correct attitude,grasp the laws scientifically,study new situations,inspiring motivation,broaden work channels,and improve efficiency.Therefore,student-affairs-administrators must enhance their own quality and competency through thoroughly studying the characteristics of college students1 ideological work,scientifically grasping the ideological and political laws,and constantly improving the pertinence and effectiveness of ideological and political education. | Hongde Gao Huabiao Wang Yanli Zhang Lirui Niu | 2019 | Journal of Contemporary Educational Research2019，3，5： | 0 |
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