|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Establishment and evaluation of a peanut association panel and analysis of key nutritional traits显示文摘Breeding programs aim to improve the yield and quality of peanut(Arachis hypogaea L.); using association mapping to identify genetic markers linked to these quantitative traits could facilitate selection efficiency. A peanut association panel was established consisting of 268 lines with extensive phenotypic and genetic variation, meeting the requirements for association analysis. These lines were grown over 3 years and the key agronomic traits, including protein and oil content were examined. Population structure(Q) analysis showed two subpopulations and clustering analysis was consistent with Q-based membership assignment and closely related to botanical type. Relative Kinship(K) indicated that most of the panel members have no or weak familial relatedness, with 52.78% of lines showing K ? 0. Linkagedisequilibrium(LD) analysis showed a high level of LD occurs in the panel. Model comparisons indicated false positives can be effectively controlled by taking Q and K into consideration and more false positives were generated by K than Q. A preliminary association analysis using a Q t K model found markers significantly associated with oil, protein, oleic acid, and linoleic acid, and identified a set of alleles with positive and negative effects. These results show that this panel is suitable for association analysis,providing a resource for marker-assisted selection for peanut improvement. | Xiurong Zhang Suqing Zhu Kun Zhang Yongshan Wan Fengzhen Liu Qingfang Sun Yingjie Li | 2018 | Journal of Integrative Plant Biology2018，60，3： | 5 |
| 2 | Molecular analysis of the chloroplast Cu/Zn-SOD gene(AhCSD2) in peanut显示文摘Superoxide dismutase(SOD, EC 1.15.1.1) plays a key role in response to drought stress, and differences in SOD activity changes among cultivars are important under drought conditions. We obtained the full-length DNA of the chloroplast Cu/Zn-SOD gene(Ah CSD2)from 11 allotetraploid cultivars and 5 diploid wild species in peanut. BLAST search against the peanut genome showed that the Ah CSD2 genes g CSD2-1 and g CSD2-2 are located at the tops of chromosome A03(A genome) and B03(B genome), respectively, and both contain 8exons and 7 introns. Nucleotide sequence analyses indicated that g CSD2-2 sequences were identical among all the tested cultivars, while g CSD2-1 sequences showed allelic variations.The amino acid sequences deduced from g CSD2-1 and g CSD2-2 both contain a chloroplast transit peptide and are distinguished by 6 amino acid(aa) residue differences. The other 2aa residue variations in the mature peptide regions give rise to three-dimensional structure changes of the protein deduced from the genes g CSD2-1 and g CSD2-2. Sequences analyses of cultivars and wild species showed that g CSD2-2 of Arachis hypogaea and g Aip CSD2(Arachis ipaensis) are identical, and despite the abundant polymorphic loci between g CSD2-1 of A.hypogaea and sequences from A genome wild species, the deduced amino acid sequence of Ah CSD2-1(A. hypogaea) is identical to that of Adu CSD2(Arachis duranensis), whereas Aco CSD2(Arachis correntina) and Aca CSD2(Arachis cardenasii) both have 2 aa differences in the transit peptide region compared with Ah CSD2-1(A. hypogaea). Based on the Peanut Genome Project, promoter prediction revealed many stress-related cis-acting elements within the potential promoter regions(pp-A and pp-B). pp-A contains more binding sites for drought-associated transcriptional factors than pp-B. We hypothesize that the marked changes in SOD activity in different cultivars under drought stress are tightly regulated by transcription factors through transcription and expression of Ah CSD2 genes. | Xiurong Zhang Qian Wan Fengzhen Liu Kun Zhang Aiqing Sun Bing Luo Li Sun Yongshan Wan | 2015 | The Crop Journal2015，3，3： | 4 |
| 3 | Erosive Wear and Wear Mechanism of in situ TiC_P/Fe Composites显示文摘The base structure of in situ TiCp/Fe composites fabricated under industrial condition was changed by differentheat treatments. Erosive wear tests were carried out and the results were compared with that of wear-resistant whitecast iron. The results suggest that the wear resistance of the in situ TiCP/Fe composite is higher than that ofwear-resistant white cast iron under the sand erosive wear condition. The wear mechanism of the wear-resistantwhite cast iron was a cycle process that base surface was worn and carbides were exposed, then carbides was brokenand wear pits appeared. While the wear mechanism of in situ TiCP/Fe composite was a cycle process that basesurface was worn and TiC grains were exposed and dropped. The wear resistance of in situ TiCP/Fe composite waslower than that of wear-resistant white cast iron under the slurry erosive wear condition. Under such circumstance,the material was not only undergone erosive wear but also electrochemistry erosion due to the contact with waterin the medium. The wear behaviours can be a combination of two kinds of wear and the sand erosive wear is worsethan slurry erosive wear. | Zhaojing LIU, Zhiliang NING , Fengzhen LI, Xiurong YAO and Shanzhi RENSchool of Materials Science and Engineering, Harbin University of Science and Technology, Harbin 150080, China | 2005 | Journal of Materials Science & Technology2005，21，5： | 3 |
| 4 | Effects of Exogenous Nitric Oxide and 24-Epibrassinolide on the Physiological Characteristics of Peanut Seedlings Under Cadmium Stress显示文摘Cadmium(Cd) is highly toxic to plants, animals, and humans. Limited information is available on the role of nitric oxide(NO)and/or 24-epibrassinolide(EBR) in response of plants to Cd stress. In this study, a hydroponic experiment was performed to investigate the effects of NO and/or EBR on peanut plants subjected to Cd stress(200 μmol L^(-1)) with sodium nitroprusside(SNP, an exogenous NO donor)(250 μmol L^(-1)) and/or EBR(0.1 μmol L^(-1)) addition. The results showed that Cd exposure inhibited plant growth, and this stress was alleviated by exogenous NO or EBR, and especially the combination of the two. Treatment with Cd inhibited the growth of peanut seedlings, decreased chlorophyll content, and significantly increased the Cd concentration in plants. Furthermore, the concentration of reactive oxygen species(ROS) markedly increased in peanut seedlings under Cd stress, resulting in the accumulation of malondialdehyde(MDA) and proline in leaves and roots. Under Cd stress, applications of SNP, EBR, and especially the two in combination significantly reduced the translocation of Cd from roots to leaves, increased the chlorophyll content, decreased the concentrations of ROS, MDA, and proline, and significantly enhanced the activities of superoxide dismutase(SOD), peroxidase(POD), and catalase(CAT) in peanut seedlings. Exogenous NO and/or EBR also stimulated the activities of nitrate reductase(NR)and nitric oxide synthase(NOS) and increased the contents of antioxidants, such as ascorbic acid(AsA) and reduced glutathione(GSH). Furthermore, exogenous NO and/or EBR enhanced Cd accumulation in the cell wall and thus decreased Cd distribution in the organelles in the roots. The concentrations of calcium(Ca), iron(Fe), magnesium(Mg), and zinc(Zn) were also regulated by exogenous NO or EBR, and especially by the two in combination. These results indicated that SNP and EBR, alone and particularly in combination, can mitigate the negative effects of Cd stress in peanut plants. | DONG Yuanjie CHEN Weifeng BAI Xiaoying LIU Fengzhen WAN Yongshan | 2019 | Pedosphere2019，29，1： | 3 |
| 5 | Determining the critical size of a rabbit rib segmental bone defect model显示文摘In order to establish and standardize the rabbit rib segmental bone defect model,it is of vital importance to determine rabbit rib critical size defect(CSD).According to the general time needed for spontaneous long-bone regeneration,three-month observation period was set to determine the CSD.The rabbit rib segmental bone defects with different sizes from 1 to 5cm with or without periosteum were performed in the eighth rib of 4-month-old male New Zealand rabbits and underwent Xray examinations at the 4th,8th and 12th weeks postoperatively.The gross and histological examinations at postoperative week 12 were evaluated,which showed that the critical sizes in the rabbit rib models with and without periosteum were 5 and 2 cm,respectively.This study provides prerequisite data for establishing rabbit rib CSD model and evaluating bonematerials using this model. | Fengzhen Liu Kun Chen Lei Hou Keyi Li Dawei Wang Bin Zhang Xiumei Wang | 2016 | Regenerative Biomaterials2016，3，5： | 2 |
| 6 | Nanobody-based chimeric antigen receptor T cells designed by CRISPR/Cas9 technology for solid tumor immunotherapy显示文摘Chimeric antigen receptor-based T-cell immunotherapy is a promising strategy for treatment of hematological malignant tumors;however,its efficacy towards solid cancer remains challenging.We therefore focused on developing nanobody-based CAR-T cells that treat the solid tumor.CD105 expression is upregulated on neoangiogenic endothelial and cancer cells.CD105 has been developed as a drug target.Here we show the generation of a CD105-specific nanobody,an anti-human CD105 CAR-T cells,by inserting the sequences for anti-CD105 nanobody-linked standard cassette genes into AAVS1 site using CRISPR/Cas9 technology.Co-culture with CD105+target cells led to the activation of anti-CD105 CAR-T cells that displayed the typically activated cytotoxic T-cell characters,ability to proliferate,the production of pro-inflammatory cytokines,and the specific killing efficacy against CD105+target cells in vitro.The in vivo treatment with anti-CD105 CAR-T cells significantly inhibited the growth of implanted CD105+tumors,reduced tumor weight,and prolonged the survival time of tumor-bearing NOD/SCID mice.Nanobody-based CAR-T cells can therefore function as an antitumor agent in human tumor xenograft models.Our findings determined that the strategy of nanobody-based CAR-T cells engineered by CRISPR/Cas9 system has a certain potential to treat solid tumor through targeting CD105 antigen. | Fengzhen Mo Siliang Duan Xiaobing Jiang Xiaomei Yang Xiaoqiong Hou Wei Shi Cueva Jumbo Juan Carlos Aiqun Liu Shihua Yin Wu Wang Hua Yao Zihang Yu Zhuoran Tang Shenxia Xie Ziqiang Ding Xinyue Zhao Bruce D.Hammock Xiaoling Lu | 2021 | Signal Transduction and Targeted Therapy2021，6，3： | 2 |
| 7 | Amorphous silicon nitride deposited by hot - wire chemical vapor deposition 显示文摘 | Fengzhen Liu Scott Ward Lynn Gedvilas | 2004 | J Applied Physics2004，96，： | 1 |
| 8 | Nano-Structure in micro-crystalline silicon-angle X-ray scattering显示文摘 | Zhou Bingqing Liu Fengzhen Gu Jinhua | 2006 | Thin Solid Films2006，501，： | 1 |
| 9 | Synergically enhanced piezocatalysis performance of eco-friendly(K_(0.52)Na_(0.48))NbO_(3) through ferroelectric polarization and defects显示文摘Piezocatalysis has attracted unprecedented research interest as a newly emerging catalysis technology.However,the inherent insulating property of ferroelectric materials ultimately leads to the poor vibration-electricity conversion ability.Herein,this work reports the(K_(0.52)Na_(0.48))NbO_(3) ferroelectric ceramics(KNNFCx),for which the FeCo modification strategy is proposed.The substitution of the moderate amount of FeCo(x=0.015)at Nb site not only optimizes ferroelectricity but also produces beneficial defects,notably increasing Rhodamine B water purification efficiency to 95%.The pinning effect of monovalent oxygen vacancies on ferroelectric domains is responsible for the excellent ferroelectric polarization of KNNFC0.015 through the generation of an internal field to promote charge carriers separation and reduce nonradiative recombination.Importantly,the accompanying electron carriers can easily move to the material surface and participate in redox reactions because they have low activation energy.Therefore,ferroelectric polarization and defects play synergetic roles in enhancing piezocatalytic performance. | Min Zhou Laijun Liang Dingze Lu Xiaomei Lu Zheng Wang Fengzhen Huang Pengfei Cheng Dongdong Liu Mengqi Tian Qiuping Wang Yunjie Zhang | 2023 | International Journal of Minerals,Metallurgy and Materials2023，30，10： | 1 |
| 10 | 9 μm cutoff 128×128A/GaAs/GaAs Quantum Wbn Infrared Photodetector Focal Plane Array显示文摘 | LIU Ying-bin FENGZhen | 2006 | Chinese Journal of Semiconductor2006，27，8： | 1 |
| 11 | High rate deposition of microcrystalline silicon films by high-pressure radio frequency plasma enhanced chemical vapor deposition (PECVD)显示文摘Hydrogenated microcrystalline silicon (μc-Si:H) thin films were prepared by high- pressure radio-frequency (13.56 MHz) plasma enhanced chemical vapor deposition (rf-PECVD) with a screened plasma. The deposition rate and crystallinity varying with the deposition pressure, rf power, hydrogen dilution ratio and electrodes distance were systematically studied. By optimizing the deposition parameters the device quality μc-Si:H films have been achieved with a high deposition rate of 7.8 /s at a high pressure. The Voc of 560 mV and the FF of 0.70 have been achieved for a single-junction μc-Si:H p-i-n solar cell at a deposition rate of 7.8 /s. | ZHOU BingQing ZHU MeiFang LIU FengZhen LIU JinLong ZHOU YuQin LI GuoHua DING Kun | 2008 | Science China(Technological Sciences)2008，51，4： | 1 |
| 12 | Novel pre-vascularized tissue-engineered dermis based on stem cell sheet technique used for dermis-defect healing显示文摘Insufficient donor dermis and the shortage of three-dimensional vascular networks are the main limitations in the tissue-engineered dermis(TED).To solve these problems,we initially constructed pre-vascularized bone marrow mesenchymal stem cell sheet(PBMCS)and pre-vascularized fibroblasts cell sheet(PFCS)by cell sheet technology,and then superimposed or folded them together to construct a pre-vascularized TED(PTED),aiming to mimic the real dermis structure.The constructed PTED was implanted in nude mice dorsal dermis-defect wound and the wound-healing effect was quantified at Days 1,7 and 14 via the methods of histochemistry and immunohistochemistry.The results showed that PTED could rapidly promote the wound closure,especially at Day 14,and the wound-healing rate of three-layer PTED could reach 97.2%(P<0.01),which was faster than the blank control group(89.1%),PBMCS(92.4%),PFCS(93.8%)and six-layer PTED(92.3%).In addition,the vessel density in the PTED group was higher than the other groups on the 14th day.Taken together,it is proved that the PTED,especially three-layer PTED,is more conducive to the fullthickness dermis-defect repair and the construction of the three-dimensional vascular networks,indicating its potential application in dermis-defect repair. | Zengjie Fan Xuzhuzi Xie Shengqian Zhu Xiaozhu Liao Zhengrong Yin Yujue Zhang Fengzhen Liu | 2020 | Regenerative Biomaterials2020，7，6： | 0 |
| 13 | An improved high-oleic line created by chemical mutagen of ‘Huayu 40’显示文摘By using chemical mutagenesis, ‘Huayu 40’, a normal-oleic(NO) large-seed high yielding peanut cultivar with wide adaptability was transformed into mutants with desirable high-oleic(HO) quality traits, comparable productivity and adaptability. Of the 3 chemical treatments, viz. 5 mM ethyl methane sulfonate(EMS), 25 mM EMS and 15 mM sodium azide(NaN_3), only NaN_3 produced HO M_2 mutant plants. High oleate phenotype was initially identified by near infrared spectroscopy(NIRS) screening, and was further confirmed by gas spectrometry(GC) analysis. Sequence analysis showed that HO plant had a 448 G>A mutation in FAD2 A and a 441_442 insA mutation in FAD2 B, respectively. Although the direct use of high yielding and stress resistant cultivars/lines to induce quality mutations might be a judicious choice to accelerate breeding, our results showed that chemical mutagenesis could quickly create HO peanut materials and expand the genetic bases of HO peanuts. | Xiuzhen Wang Weiguang Ning Quanxi Sun Yueyi Tang Zhiwei Wang Yongshan Wan Fengzhen Liu Zubo Du Feng Liu Qi Wu Chuantang Wang | 2019 | Oil Crop Science2019，4，1： | 0 |
| 14 | CDCP:a visualization and analyzing platform for single-cell datasets显示文摘Recently,the rapid advances of single-cell sequencing technologies,including sequencing of single-cell genomics,transcriptomes,epigenetics,and spatial transcriptomes,have empowered researchers to scrutinize cellular heterogeneity,gene expression,epigenetic modifications. | Yuejiao Li Tao Yang Tingting Lai Lijin You Fan Yang Jiaying Qiu Lina Wang Wensi Du Cong Hua Zhicheng Xu Jia Cai Zhiyong Li Yiqun Liu Ling Li Minwen Zhang Jing Chen Lei Zhang Dongsheng Chen Weiwen Wang Shiping Liu Liang Wu Wenjun Zeng Bo Wang Xiaofeng Wei Longqi Liu Fengzhen Chen | 2022 | Journal of Genetics and Genomics2022，49，7： | 0 |
| 15 | MECHANISM OF Re-B-V CO-PERMEATION显示文摘MECHANISMOFReBVCOPERMEATION①LiFengzhen,LiuZhaojing,JinQuan,XuZhihuiDepartmentofMaterialsScienceandEngineering,HarbinUniver... | Li, Fengzhen Liu, Zhaojing Jin, Quan Xu, Zhihui | 1997 | 中国有色金属学会会刊:英文版1997，7，1： | 0 |
| 16 | Tapered Optical Fiber-Based LSPR Biosensor for Ascorbic Acid Detection显示文摘The ascorbic acid(AA)is a biomarker that can be used to detect the symptoms of severe disorders such as scurvy,Parkinson’s,Alzheimer’s,and cardiovascular diseases.In this work,a simple and effective sensor model is developed to diagnose the presence of AA samples.To develop the sensor,a tapered single-mode optical fiber has been used with the well-known phenomenon of localized surface plasmon resonance(LSPR).For LSPR,the tapered region is immobilized with synthesized gold nanoparticles(AuNPs)and zinc oxide nanoparticles(ZnO-NPs)whose absorbance peak wavelengths appear at 519nm and 370nm,respectively.On the basis of nanoparticles(NPs)configurations,two different biosensor probes are developed.In the first one,the sensing region is immobilized with AuNPs and named Probe I.In the second probe,the immobilized layer of AuNPs is further coated with a layer of ZnO-NPs,and a resultant probe is termed as Probe II.The characterizations of synthesized AuNPs and developed fiber probes are done by the ultraviolet-visible(UV-vis)spectrophotometer,high-resolution transmission electron microscope(HR-TEM),atomic force microscopy(AFM),and scanning electron microscope(SEM).To enhance the selectivity,a sensing region of probes is functionalized with ascorbate oxidase enzyme that oxidizes the AA in the presence of oxygen.The response of developed sensor probes is authenticated by sensing the samples of AA in the range from 500 nM to 1 mM,which covers the range of AA found in human bodies,i.e.,40μM-120μM.The performance analysis of the developed sensor probes has been done in terms of their stability,reproducibility,reusability,and selectivity.To observe the stability of AA,a pH-test has also been done that results in a better solubility of AA molecules in phosphate-buffered saline(PBS)solution. | Guo ZHU Lokendra SINGH Yu WANG Ragini SINGH Bingyuan ZHANG Fengzhen LIU Brajesh Kumar KAUSHIK Santosh KUMAR | 2021 | Photonic Sensors2021，11，4： | 0 |
| 17 | Integrin α_(v)β_(3)-targeted polydopamine-coated gold nanostars for photothermal ablation therapy of hepatocellular carcinoma显示文摘Photothermal therapy(PTT)has emerged as a promising cancer therapeutic method.In this study,Arg-Gly-Asp(RGD)peptide-conjugated polydopamine-coated gold nanostars(Au@PDA-RGD NPs)were prepared for targeting PTT of hepatocellular carcinoma(HCC).A polydopamine(PDA)shell was coated on the surface of gold nanostars by the oxidative self-polymerization of dopamine(termed as Au@PDA NPs).Au@PDA NPs were further functionalized with polyethylene glycol and RGD peptide to improve biocompatibility as well as selectivity toward the HCC cells.Au@PDARGD NPs showed an intense absorption at 822 nm,which makes them suitable for near-infraredexcited PTT.Our results indicated that the Au@PDA-RGD NPs were effective for the PTT therapy of the α_(v)β_(3) integrin receptor-overexpressed HepG2 cells in vitro.Further antitumor mechanism studies showed that the Au@PDA-RGD NPs-based PTT induced human liver cancer cells death via the mitochondrial-lysosomal and autophagy pathways.In vivo experiments showed that Au@PDARGD NPs had excellent tumor treatment efficiency and negligible side effects.Thus,our study showed that Au@PDA-RGD NPs could offer an excellent nanoplatform for PTT of HCC. | Yang Li Ping Hu Xiali Wang Xu Hou Fengzhen Liu Xiaohong Jiang | 2021 | Regenerative Biomaterials2021，8，5： | 0 |
| 18 | Maternal Vascular Dysfunction in Congenital Heart Defects显示文摘Background:Research on fetal congenital heart defect(CHD)mostly focuses on etiology and mechanisms.However,studies on maternal complications or pathophysiology are limited.Our objective was to determine whether vascular dysfunction exists in pregnant women carrying a fetus with congenital heart defects.Methods:We conducted a case-control study.27 cases of pregnant women carrying a fetus with major CHD admitted to our hospital for delivery between April 2021 and August 2022 were selected.Every case was matched with about 2 pregnant complication-free controls without fetal abnormalities.The proangiogenic and anti-angiogenic factors and pregnancy outcomes were compared.Results:The proangiogenic factors include vascular endothelial growth factor(VEGF)and placental growth factor(PlGF).The anti-angiogenic factors involve soluble fms-like tyrosine kinase 1(sFlt-1)and soluble endoglin(sEng).No differences were found in maternal plasma concentrations of PlGF,VEGF,and sFlt-1 between case-control groups when analyzed at 36 weeks≤gestational age(GA)<39 weeks and 39 weeks≤GA≤41 weeks.The concentrations of sEng in maternal plasma in the fetal CHD group were significantly higher than those in the control group:0.60(0.77)vs.0.32(0.26)ng/ml at 36 weeks≤GA<39 weeks,p=0.001 and 0.75(0.55)vs.0.28(0.27)ng/ml at 39 weeks≤GA≤41 weeks,p<0.001.Conclusion:Vascular dysfunction exists in pregnant women with fetal congenital heart defects,manifesting significantly elevated sEng concentration at delivery. | Yanli Liu Fengzhen Han Jian Zhuang Yanqiu Ou Yanji Qu Yanyan Lin Weina Zhang Haiping Wang Liping Huang | 2023 | Congenital Heart Disease2023，18，5： | 0 |
您所在的机构暂未开通此部分的功能，请联系管理员