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102篇 您的检索式:作者名="Shiping Liu"
    题名 作者 年代 出处 被引量
1Rice Gene Network Inferred from Expression Profiling of Plants Overexpressing OsWRKY13, a Positive Regulator of Disease Resistance显示文摘积累的信息显示植物疾病电阻发信号小径经常与调整发展过程或不能生活的压力回答的另外的小径交往。然而,串音的这些类型的分子的机制仍然保持糟糕在大多数情况中理解。这里,我们报导那 OsWRKY13,到细菌、真菌的病原体的米饭电阻的使活跃之物,看起来在导致病原体的水杨酸盐依赖者防卫小径和五条另外的生理的小径之中为串音作为一个会聚的点工作。OsWRKY13-overexpressing 线的表达式侧面的染色体宽的分析建议 OsWRKY13 直接或间接地调整潜在地根据基因本体论数据库的分类涉及不同生理的进程的超过 500 基因的表达式。由比较在已知的小径或在 OsWRKY13-overexpressing 和野类型的植物之间的病原体感染和显型的细胞的过程工作的基因的表示模式,我们的数据建议 OsWRKY13 也在病原体感染期间是另外的生理的过程的一个管理者。联系 OsWRKY13 的疾病抵抗小径 synergistically 与 glutathione/glutaredoxin 系统和 flavonoid 生合成小径经由 OsWRKY13 交往监视氧化还原作用动态平衡并且分别地,通常认为地在 OsWRKY13-overexpressing 线提高抗菌剂 flavonoid phytoalexins 的生合成。同时,联系 OsWRKY13 的疾病抵抗小径看起来经由 OsWRKY13 与调停 SNAC1 的不能生活的压力防卫小径, jasmonic 酸发信号小径,和 terpenoid 新陈代谢小径反对地交往压制盐和冷防卫回答以及通常认为地延迟米饭生长和开发。Deyun Qiu Jun Xiao Weibo Xie Hongbo Liu Xianghua Li Lizhong Xiong Shiping Wang 2008Molecular Plant2008,1,3:38
2Terrestrial carbon sinks in China and around the world and their contribution to carbon neutrality显示文摘Enhancing the terrestrial ecosystem carbon sink(referred to as terrestrial C sink) is an important way to slow down the continuous increase in atmospheric carbon dioxide(CO_(2)) concentration and to achieve carbon neutrality target.To better understand the characteristics of terrestrial C sinks and their contribution to carbon neutrality,this review summarizes major progress in terrestrial C budget researches during the past decades,clarifies spatial patterns and drivers of terrestrial C sources and sinks in China and around the world,and examines the role of terrestrial C sinks in achieving carbon neutrality target.According to recent studies,the global terrestrial C sink has been increasing from a source of (-0.2±0.9) Pg C yr^(-1)(1 Pg=1015g)in the 1960s to a sink of (1.9±1.1) Pg C yr^(-1) in the 2010s.By synthesizing the published data,we estimate terrestrial C sink of 0.20–0.25 Pg C yr^(-1) in China during the past decades,and predict it to be 0.15–0.52 Pg C yr^(-1) by 2060.The terrestrial C sinks are mainly located in the mid-and high latitudes of the Northern Hemisphere,while tropical regions act as a weak C sink or source.The C balance differs much among ecosystem types:forest is the major C sink;shrubland,wetland and farmland soil act as C sinks;and whether the grassland functions as C sink or source remains unclear.Desert might be a C sink,but the magnitude and the associated mechanisms are still controversial.Elevated atmospheric CO_(2) concentration,nitrogen deposition,climate change,and land cover change are the main drivers of terrestrial C sinks,while other factors such as fires and aerosols would also affect ecosystem C balance.The driving factors of terrestrial C sink differ among regions.Elevated CO_(2) concentration and climate change are major drivers of the C sinks in North America and Europe,while afforestation and ecological restoration are additionally important forcing factors of terrestrial C sinks in China.For future studies,we recommend the necessity for intensive and long-term ecosystem C monitoring over broad geographic scale to improve terrestrial biosphere models for accurately evaluating terrestrial C budget and its dynamics under various climate change and policy scenarios.Yuanhe Yang Yue Shi Wenjuan Sun Jinfeng Chang Jianxiao Zhu Leiyi Chen Xin Wang Yanpei Guo Hongtu Zhang Lingfei Yu Shuqing Zhao Kang Xu Jiangling Zhu Haihua Shen Yuanyuan Wang Yunfeng Peng Xia Zhao Xiangping Wang Huifeng Hu Shiping Chen Mei Huang Xuefa Wen Shaopeng Wang Biao Zhu Shuli Niu Zhiyao Tang Lingli Liu Jingyun Fang 2022Science China(Life Sciences)2022,65,5:32
3Adventitious root formation in rice requires OsGNOM1 and is mediated by the OsPINs family显示文摘在谷物的含纤维的根系统包括首先偶然根(艺术) ,它在营养素和水举起起重要作用。关于位于 AR 开发下面的分子的机制的当前的知识仍然是有限的。我们这里报导四米饭(Oryza sativa L.) 的隔离异种从不同基因背景,所有哪个在 AR 是有缺点的形成。这些异种展出了侧面的根(LR ) 和 gravitropism 的部分损失的减少的数字。也显示的异种提高了敏感到 N-1-naphthylphthalamic 酸,极的植物生长素运输的一个禁止者(轻拍) ,显示变化影响了植物生长素运输。用四异种之一的位置的克隆表明它被一个 guanine 核苷酸交换因素的 loss-of-function 为 ADP-ribosylation 因素(OsGNOM1 ) 引起。转基因的植物显示出的 promoter::GUS 的 RT-PCR 和分析那 OsGNOM1 在 AR primordia,脉管的纸巾, LR,根尖端,叶子,花药和词根静脉被表示,与类似于植物生长素的一个分发模式。另外, OsPIN2, OsPIN5b 和 OsPIN9 的表情在异种被改变。一起拿,这些调查结果显示 OsGNOM1 通过调整影响艺术的形成轻拍。Shiping Liu Jirong Wang Lu Wang Xiaofei Wang Yanhong Xue Ping Wu Huixia Shou 2009Cell Research2009,19,9:29
4Whole-genome sequencing of 508 patients identifies key molecular features associated with poor prognosis in esophageal squamous cell carcinoma显示文摘Esophageal squamous cell carcinoma(ESCC)is a poor-prognosis cancer type with limited understanding of its molecular etiology.Using 508 ESCC genomes,we identified five novel significantly mutated genes and uncovered mutational signature clusters associated with metastasis and patients’outcomes.Several functional assays implicated that NFE2L2 may act as a tumor suppressor in ESCC and that mutations in NFE2L2 probably impaired its tumor-suppressive function,or even conferred oncogenic activities.Additionally,we found that the NFE2L2 mutations were significantly associated with worse prognosis of ESCC.We also identified potential noncoding driver mutations including hotspot mutations in the promoter region of SLC35E2 that were correlated with worse survival.Approximately 5.9%and 15.2%of patients had high tumor mutation burden or actionable mutations,respectively,and may benefit from immunotherapy or targeted therapies.We found clinically relevant coding and noncoding genomic alterations and revealed three major subtypes that robustly predicted patients’outcomes.Collectively,we report the largest dataset of genomic profiling of ESCC useful for developing ESCC-specific biomarkers for diagnosis and treatment.Yongping Cui Hongyan Chen Ruibin Xi Heyang Cui Yahui Zhao Enwei Xu Ting Yan Xiaomei Lu Furong Huang Pengzhou Kong Yang Li Xiaolin Zhu Jiawei Wang Wenjie Zhu Jie Wang Yanchun Ma Yong Zhou Shiping Guo Ling Zhang Yiqian Liu Bin Wang Yanfeng Xi Ruifang Sun Xiao Yu Yuanfang Zhai Fang Wang Jian Yang Bin Yang Caixia Cheng Jing Liu Bin Song Hongyi Li Yi Wang Yingchun Zhang Xiaolong Cheng Qimin Zhan Yanhong Li Zhihua Liu-Show 2020Cell Research2020,30,10:19
5Plant phenological responses to climate change on the Tibetan Plateau: research status and challenges显示文摘Phenology studies the cycle of events in nature that are initiated and driven by an annually recurring environment. Plant phenology is expected to be one of the most sensitive and easily observable natural indicators of climate change. On the Tibetan Plateau(TP), an accelerated warming since the mid-1980 s has resulted in signiicant environmental changes. hese new conditions are accompanied by phenological changes that are characterized by considerable spatiotemporal heterogeneity. Satellite remote sensing observed widespread advance in the start of the plant growing season across the plateau during the 1980 s and 1990 s but substantial delay over 2000–2011 in the southwest although it continued to advance in the northeast regions of the TP. Both observational studies and controlled experiments have revealed, to some extent, the positive role of higher preseason temperature and even more precipitation in advancing the leaf onset and irst lowering date of the TP. However, a number of rarely visited research issues that are essential for understanding the role of phenology in ecosystem responses and feedback processes to climate change remain to be solved. Our review recommends that addressing the following questions should be a high priority. How did other phenological events change, such as lowering and fruiting phenology? What are the inluences from environmental changes other than temperature and precipitation, including human activities such as grazing? What are the genetic and physiological bases of plants phenological responses?How does phenological change inluence ecosystem structure and function at diferent scales and feedback to the climate system? Investigating these research questions requires, irst of all, new data of the associated environmental variables, and consistent and reliable phenological observation using diferent methodologies(i.e. in situ observations and remote sensing).Miaogen Shen Shilong Piao Tsechoe Dorji Qiang Liu Nan Cong Xiaoqiu Chen Shuai An Shiping Wang Tao Wang Gengxin Zhang 2015National Science Review2015,2,4:13
6Production of β-1,3-glucanase and chitinase of two biocon-trol agents and their possible modes of action显示文摘Pichia membranefaciens Hansen and Candida guilliermondii (Cast) Langeronet Guerra are two antagonists of R. stolonifer on harvested nectarine and peach fruits. In this study, β-1,3-glucanase and chitinase activities of the antagonists were induced in vitro and in vivo. The highest β-1, 3-glucanase activity was detected in Lilly-Barnett minimal salt medium supplemented with glucose in combination with CWP of R. stolonifer as a carbon source. The β-1,3-glucanase activity of P. membranefaciens reached the maximum level, being 114.0 SU (specific activity unit), and that of C. guilliermondii reached 103.2 SU. The lowest β-1,3-glucanase activity was observed in the medium containing glucose as sole car-bon source. P. membranefaciens was able to produce signifi-cantly higher levels of chitinase (exochitinase and endochiti-nase) in vitro than C. guilliermondii grown in Czapeck mini-mal medium. An increase in β-1,3-glucanase and chitinase activity was also triggered by wounding, adding of carbon sourcesFAN Qing, TIAN Shiping, LIU Haibo & XU YongInstitute of Botany, Chinese Academy of Sciences, Beijing 100093, China 2002Chinese Science Bulletin2002,47,4:11
7Internet of Things to network smart devices for ecosystem monitoring显示文摘Smart, real-time, low-cost, and distributed ecosystem monitoring is essential for understanding and managing rapidly changing ecosystems. However, new techniques in the big data era have rarely been introduced into operational ecosystem monitoring, particularly for fragile ecosystems in remote areas.We introduce the Internet of Things(IoT) techniques to establish a prototype ecosystem monitoring system by developing innovative smart devices and using IoT technologies for ecosystem monitoring in isolated environments. The developed smart devices include four categories: large-scale and nonintrusive instruments to measure evapotranspiration and soil moisture, in situ observing systems for CO2 and d13 C associated with soil respiration, portable and distributed devices for monitoring vegetation variables, and Bi-CMOS cameras and pressure trigger sensors for terrestrial vertebrate monitoring. These new devices outperform conventional devices and are connected to each other via wireless communication networks. The breakthroughs in the ecosystem monitoring IoT include new data loggers and longdistance wireless sensor network technology that supports the rapid transmission of data from devices to wireless networks. The applicability of this ecosystem monitoring IoT is verified in three fragile ecosystems, including a karst rocky desertification area, the National Park for Amur Tigers, and the oasis-desert ecotone in China. By integrating these devices and technologies with an ecosystem monitoring information system, a seamless data acquisition, transmission, processing, and application IoT is created. The establishment of this ecosystem monitoring IoT will serve as a new paradigm for ecosystem monitoring and therefore provide a platform for ecosystem management and decision making in the era of big data.Xin Li Ning Zhao Rui Jin Shaomin Liu Xiaomin Sun Xuefa Wen Dongxiu Wu Yan Zhou Jianwen Guo Shiping Chen Ziwei Xu Mingguo Ma Tianming Wang Yonghua Qu Xinwei Wang Fangming Wu Yuke Zhou 2019Science Bulletin2019,64,17:10
8RAPID AND NONDESTRUCTIVE MEASUREMENT SYSTEM FOR WELDING RESDlUAL STRESS BY ULTRASONIC METHOD显示文摘Traditional methods of residual stress measurement are generally destructive or semi-destructive,as well as expensive,time-consuming and complex to implement.With the new development of welded structure,traditional methods can not satisfy the need of full life task management.So the acoustical theory is introduced,since the ultrasonic technique provides a useful nondestructive tool in the evaluation of stresses.In this study an ultrasonic stress measurement experimental installation is established,which consists of a special transducer,a signal emission unit and a signal recipient processing unit.Longitudinal critically refracted wave is selected as the measurement wave mode.The supporting software is programmed by Labview software.The longitudinal residual stress and transverse residual stress of twin wire welded plate are measured by this installment,in which the measuring process is real-time,quick and nondestructive.The experiment results indicate that the system can satisfy the need of life evaluation for welded structure. The system is light and portable.LU Hao LIU Xuesong ZHU Zheng ZHANG Shiping FANG Hongyuan 2008Chinese Journal of Mechanical Engineering2008,21,6:8
9Mathematical Model of Hydrodynamic Torque Converter and Analytic Description of Streamline显示文摘The mathematical model of a 3-element centripetal-turbine hydrodynamic torque converter and analytic description of fluid flow inside the hydrodynamic torque converter are investigated.A new torus coordinate system is proposed so as to quantitatively describe fluid movement inside the hydrodynamic torque converter.The particle movement inside the hydrodynamic torque converter is decomposed into meridional component movement and torus component movement, and a universal meridional streamline equation is derived.According to the relationship between the converter wheel velocity polygon and its blade angle, a torus streamline differential equation is established.The universal meridional streamline equation is approximated with square polynomials.The approximation error curve is given and the percentage error is not greater than 0.86%.Considered as a function of polar angle, the blade angle cotangent of each converter wheel varies linearly with polar angle.By using integral calculus, torus streamline equations are obtained.As a result, the problem of difficult flow description of the hydrodynamic torque converter is solved and a new analytic research system is established.LIU Shiping QUAN Long 2009Chinese Journal of Mechanical Engineering2009,22,1:7
10Vaccination against Schistosoma japonicum Infection by DNA Vaccine Encoding Sj22.7 Antigen显示文摘在鼠标的效果, recombinant 原生质标志 pcDNA3-Sj22.7 与调停 liposome 的方法习惯于 transfectHeLa 房间, Sj22.7 mRNA 和蛋白质的表达式用反向的抄写聚合酶链反应,钠 dodecylsulfate-polyacrylamide 凝胶电泳和蛋白质印迹被检验。然后, pcDNA3-Sj22.7 的能力在鼠标的种痘以后根据蠕虫减小率和鸡蛋减小率被分析免于 Schistosomajaponicum 挑战感染。特定的 IgG 抗体和 T 淋巴细胞增长反应的浆液层次也是坚定的。在挑战感染以后,驾驶 Sj22.7 的干扰素(IFN )-gamma andinterleukin (IL )-4 也被确定。结果证明 pcDNA3-Sj22.7 能表示 Sj22.7 mRNAand 在 vitro.Immunization 的蛋白质导致了29.70%的蠕虫减小率;鸡蛋减小率of47.25%(肝)并且51.73%(肠),并且25.90%的鸡蛋减小率(鸡蛋每女性),在 连接group.Enzyme 的免疫吸着剂试金和蛋白质印迹分析显示了的 pcDNA3-Sj22.7 建议重要反肥沃的正式就职那使对从与 pcDNA3-Sj22.7 使免疫的鼠标的 Sj22.7.Tlymphocytes 的产生特定的 IgG 显示出的鼠标免疫怒气房间的一种重要增长反应 torSj22.7.The 文化证明 IFN-gamma 的那分泌物增加了但是 IL-4decreased.The 结果显示由 pcDNA3-Sj22.7 的 DNA 种痘对足够对 S.japonicum 感染得到保护的免疫的重要层次。DNA 疫苗能导致重要细胞、体液的有免疫力的反应,并且显示占优势的 T 助手房间类型 1 类型免疫者回答,它在鼠标对挑战感染贡献保护的免疫。Gan DAI Shiping WANG Junlong YU Shaorui XU Xianchu PENG Zhuo HE Xueqin LIU Songhua ZHOU and Fen LIU Institute of Schistosomiasis Research.Xiangya School of Medicine,Central South University,Changsha 410078 China 2007Acta Biochimica et Biophysica Sinica2007,39,1:5
11Bimetallic nano-mushrooms with DNA-mediated interior nanogaps for high-efficiency SERS signal amplification显示文摘有为散布的提高表面的拉曼(重量的单位) 分析的 ultrasensitive 的明确的 nanogaps 抓住伟人诺言的一致包含银的金属 nanostructures。不过,有强壮、稳定的重量的单位的如此的 nanostructures 的直接合成表明极其质问的遗体。这里,我们与内部 nanogaps 为金银的 nano 蘑菇的直接合成报导一条调停 DNA 的途径。这些 nano 蘑菇的重量的单位紧张极其依赖于在金头和银帽子之间的 nanogap 的区域。我们发现 nanogaps 的形成由控制在金 nanoparticles 上标记搁浅单人赛的 DNA (ssDNA ) 的 6-carboxy-X-rhodamine (ROX ) 的表面密度是细微地悦耳的。我们与 1.0 的一个高重量的单位信号改进因素在高产量获得了 nano 蘑菇我们作为一项 retrofit 措施在 CHS 在用阶段变化材料(脉冲编码调制) 的热存储的采纳以后。结果显示那与融化 23 的温度使用脉冲编码调制 ? 訄吗??Jianlei Shen Jing Su Juan Yan Bin Zhao Dongfang Wang Siyi Wang Kun Li Mengmeng Liu Yao He Sanjay Mathur Chunhai Fan Shiping Song 2015Nano Research2015,8,3:4
12Cloning, expression and protective immunity evalua-tion of the full-length cDNA encoding succinate dehydrogenase iron-sulfur protein of Schistosoma japonicum显示文摘1071-bp fragment was obtained from the Schistosoma japonicum (Chinese strain) adult cDNA library after the 3′ and 5′ ends of the incomplete expression sequence tag (EST) of succinate dehydrogenase iron-sulfur protein of Schistosoma japonicum (SjSDISP) were amplified by the anchored PCR with 2 pairs of primers designed according to the EST of SjSDISP and the sequence of multiclone sites of the library vector. Sequence analysis indicated that the fragment was a full-length cDNA with a complete open reading frame (ORF), encoding 278 amino acid residues. The fragment was cloned into prokary- otic expression vector pQE30, and subsequently sequenced and expressed in Escherichia coli. SDS-PAGE and Western-blot analyses showed that the recombinant protein was about 32 kD and could be recognized by the polyclonal antisera from rabbits immunized with Schistosoma japonicum adult worm antigen. Compared with the FCA controls, mice vaccinated with rSjSDISP (test) or rSjGST (posi- tive control) all revealed high levels of specific antibody and significant reduction in worm burden, liver eggs per gram (LEPG), fecal eggs per gram (FEPG) and intrauterine eggs. These results suggest that SjSDISP may be a novel and partially protective vaccine candidate against schistosomiasis. In contrast to the worm burden reduction rate, the higher degree of egg reduction rate in the test group also sug- gested that SjSDISP vaccine may primarily play a role in anti-embryonation or anti-fecundity immunity.YU JunLong, WANG ShiPing, LI WenKai, DAI Gan, XU ShaoRui, HE Zhuo, PENG XianChu, ZHOU SongHua & LIU XueQin Department of Parasitology, Xiangya School of Medicine, Central South University, Changsha 410078, China 2007Science China(Life Sciences)2007,50,2:4
13Characterization of a disease susceptibility locus for exploring an efficient way to improve rice resistance against bacterial blight显示文摘Bacterial blight caused by Xanthomonas oryzae pv.oryzae(Xoo) is the most harmful bacterial disease of rice worldwide.Previously,we characterized major disease resistance(MR) gene xa25,which confers race-specific resistance to Xoo strain PXO339.The xa25 is a recessive allele of the SWEET13 locus,but SWEET13's interaction with PXO339 and how efficiently using this locus for rice breeding still need to be defined.Here we show that the SWEET 13 allele from rice Zhenshan 97 is a susceptibility gene to PXO339.Using this allele's promoter to regulate xa25 resulted in disease,suggesting that the promoter is a key determinant in SWEET13 caused disease in Zhanshan 97 after PXO339 infection.PXO339 transcriptionally induces SWEET13 to cause disease.Partial suppressing SWEET13 expression leads to a high level of resistance to PXO339.Thus,the transcriptionally suppressed SWEET13 functions as xa25 in resistance to PXO339.Hybrid rice is widely grown in many countries.However,recessive MR genes have not been efficiently used for disease resistance breeding in hybrid rice production for both parents of the hybrid have to carry the same recessive gene.However,the suppressed SWEET 13 functions dominantly,which will have advantage to improve the resistance of hybrid rice to xa25-incomptible Xoo.Qi Cheng Weihua Mao Wenya Xie Qinsong Liu Jianbo Cao Meng Yuan Qinglu Zhang Xianghua Li Shiping Wang 2017Science China(Life Sciences)2017,60,3:4
14miR395-regulated sulfate metabolism exploits pathogen sensitivity to sulfate to boost immunity in rice显示文摘MicroRNAs (miRNAs) play important roles in plant physiological activities. However, their roles and molecular mechanisms in boosting plant immunity, especially through the modulation of macronutrient metabolism in response to pathogens, are largely unknown. Here, we report that an evolutionarily conserved miRNA, miR395, promotes resistance to Xanthomonas oryzae pv. oryzae (Xoo) and X. oryzae pv. oryzicola (Xoc), two destructive bacterial pathogens, by regulating sulfate accumulation and distribution in rice. Specifically, miR395 targets and suppresses the expression of the ATP sulfurylase gene OsAPS1, which functions in sulfate assimilation, and two sulfate transporter genes, OsSULTR2;1 and OsSULTR2;2, which function in sulfate translocation, to promote sulfate accumulation, resulting in broad-spectrum resistance to bacterial pathogens in miR395-overexpressing plants. Genetic analysis revealed that miR395-triggered resistance is involved in both pathogen-associated molecular pattern-triggered immunity and R gene-mediated resistance. Moreover, we found that accumulated sulfate but not S-metabolites inhibits proliferation of pathogenic bacteria, revealing a sulfate-mediated antibacterial defense mechanism that differs from sulfur-induced resistance. Furthermore, compared with other bacteria, Xoo and Xoc, which lack the sulfate transporter CysZ, are sensitive to high levels of extracellular sulfate. Accordingly, miR395-regulated sulfate accumulation impaired the virulence of Xoo and Xoc by decreasing extracellular polysaccharide production and biofilm formation. Taken together, these results suggest that rice miR395 modulates sulfate metabolism to exploit pathogen sensitivity to sulfate and thereby promotes broad-spectrum resistance.Zeyu Yang Shugang Hui Yan Lv Miaojing Zhang Dan Chen Jingjing Tian Haitao Zhang Hongbo Liu Jianbo Cao Wenya Xie Changyin Wu Shiping Wang Meng Yuan 2022Molecular Plant2022,15,4:3
15The rice Raf-like MAPKKK OsILA1 confers broad-spectrum resistance to bacterial blight by suppressing the OsMAPKK4-OsMAPK6 cascade显示文摘Mitogen-activated protein kinase kinase kinase(MAPKKK)are the first components of MAPK cascades,which play pivotal roles in signaling during plant development and physiological processes.The genome of rice encodes 75 MAPKKKs,of which 43 are Raf-like MAPKKKs.The functions and action modes of most of the Raf-like MAPKKKs,whether they function as bona fide MAPKKKs and which are their downstream MAPKKs,are largely unknown.Here,we identified the osmapkkk43 mutant,which conferred broad-spectrum resistance to Xanthomonas oryzae pv.oryzae(Xoo),the destructive bacterial pathogen of rice.Oryza sativa(Os)MAPKKK43 encoding a Raf-like MAPKKK was previously known as Increased Leaf Angle 1(OsILA1).Genetic analysis indicated that OsILA1 functioned as a negative regulator and acted upstream of the OsMAPKK4-OsMAPK6 cascade in rice-Xoo interactions.Unlike classical MAPKKKs,OsILA1 mainly phosphorylated the threonine 34 site at the N-terminal domain of OsMAPKK4,which possibly influenced the stability of OsMAPKK4.The N-terminal domain of OsILA1 is required for its homodimer formation and its full phosphorylation capacity.Taken together,our findings reveal that OsILA1 acts as a negative regulator of the OsMAPKK4-OsMAPK6 cascade and is involved in rice-Xoo interactions.Jie Chen Lihan Wang Zeyu Yang Hongbo Liu Chuanliang Chu Zhenzhen Zhang Qinglu Zhang Xianghua Li Jinghua Xiao Shiping Wang Meng Yuan 2021Journal of Integrative Plant Biology2021,63,10:3
16IMPROVE THE KINETIC PERFORMANCE OF THE PUMP CONTROLLED CLAMPING UNIT IN PLASTIC INJECTION MOLDING MACHINE WITH ADAPTIVE CONTROL STRATEGY显示文摘塑造被靠近的循环最新控制与的机器的塑料注射的夹钳的单位的运动特征开发了统一 reinvestigated 的两倍速度变量泵。就柱体等价物的宽变化而言,质量由夹钳单位和在关门的模子和开始过程期间在柱体上扮演的严重即时影响力量的传播比率引起了,参数和结构的一个适应控制原则被建议改进它的运动性能。在加速反馈获得和可变的团之间的适应关联被导出。压力微分反馈被介绍在小惯性和重影响负担的箱子中改进动态表演。到负担的和压力的改编被用来减少系统的精力损失。研究结果被模拟和实验验证。调查方法和结论对由传统的伺服泵单位控制的微分柱体系统也合适。QUAN Long LIU Shiping 2006Chinese Journal of Mechanical Engineering2006,19,1:3
17Complex Helmholtz Coils for Producing Uniform Magnetic Fields显示文摘In imaging experiments in ion-atom collisions, due to its small mass, the electron is easy to escape from the extraction electrostatic field, and this makes the complete collection difficult. In order to improve the solid angle for imaging of electrons, a uniform magnetic field is needed to restrict the electron trajectories .Usually, a pair of same coils, called Helmholtz coils, is employed to produce magnetic field. InFeng Wentian, Ma Xinwen, Liu Huiping, Chen Lanfang, Li Bin and Cao Shiping 2005近代物理研究所和兰州重离子加速器实验室年报:英文版2005,,1:3
18Tracing of dye molecules in living plants through NaGdF_4:Yb^(3+),Er^(3+)fluorescent nanoprobes显示文摘In this paper, we demonstrated trace of dye molecules in living plants. The NaGdF4:Yb^(3+),Er^(3+) nanoparticles probe was used to detect the rhodamine B(RhB) in bean sprout. It is found that the fluorescencedye can be efficiently imbibed during the growing process and the absorbance presented a position dependence effect, which was supported by the upconversion spectra and the fluorescent image characterization. In addition, the concentration of the residual RhB in bean sprout can be efficiently traced by the synthesized probe based on the fluorescent resonant energy transfer. Finally, the relation between the excitation power, concentration and the ratio of yellow to green emission are discussed in detail. These results can be helpful in understanding the RhB dye molecules absorbance process in vegetable growth and provide an efficient way to trace the residual dyes in vivo plant.Xiaofeng Wu Ya'nan Zhang Shiping Zhan Jin Li Guozheng Nie Shigang Hu Cong Yan Shaobing Wu Shengbin Cheng Junshan Hu Lichun Shi Yunxin Liu 2019Journal of Rare Earths2019,37,3:3
19A Preliminary Investigation on Phenotypic Characteristics of Phytophthora infestans from Guizhou Province显示文摘[Objective] This study aimed to investigate the phenotypic characteristics of Phytophthora infestans from Guizhou Province.[Method] The metalaxyl resistance,mating type and isozyme genotype of 292 P. infestans strains isolated from Guizhou Province were analyzed.[Result] Metalaxyl-resistant P. infestans strains were found in different areas of Guizhou Province. Resistant,moderately resistant and susceptible strains accounted for 35. 62%,33. 90% and 30. 48%,respectively. Both mating types A1 and A2 were found,which accounted for 65. 38% and 34. 62%,respectively. P. infestans strains isolated from Guizhou Province mainly belonged to Gpi genotypes 100 /100 and 100 /100 /111,accounting for 50. 68% and 43. 84%,respectively.[Conclusion]This study provides the theoretical basis for the prevention and control of potato late blight in Guizhou Province.Lu HUANG Xiaojun CHEN Haiyong HE Xuehui LIU Shiping WU 2016Agricultural Biotechnology2016,5,3:2
20Natural variations of ZmSRO1d modulate the trade-off between drought resistance and yield by affecting ZmRBOHC-mediated stomatal ROS production in maize显示文摘While crop yields have historically increased,drought resistance has become a major concern in the context of global climate change.The trade-off between crop yield and drought resistance is a common phenomenon;however,the underlying molecular modulators remain undetermined.Through genome-wide association study,we revealed that three non-synonymous variants in a drought-resistant allele of ZmSRO1d-R resulted in plasma membrane localization and enhanced mono-ADP-ribosyltransferase activity of ZmSRO1d toward ZmRBOHC,which increased reactive oxygen species(ROS)levels in guard cells and promoted stomatal closure.ZmSRO1d-R enhanced plant drought resilience and protected grain yields under drought conditions,but it led to yield drag under favorable conditions.In contrast,loss-of-function mutants of ZmRBOHC showed remarkably increased yields under well-watered conditions,whereas they showed compromised drought resistance.Interestingly,by analyzing 189 teosinte accessions,we found that the ZmSRO1d-R allele was present in teosinte but was selected against during maize domestication and modern breeding.Collectively,our work suggests that the allele frequency reduction of ZmSRO1d-R in breeding programs may have compromised maize drought resistance while increased yields.Therefore,introduction of the ZmSRO1d-R allele into modern maize cultivars would contribute to food security under drought stress caused by global climate change.Huajian Gao Junjun Cui Shengxue Liu Shuhui Wang Yongyan Lian Yunting Bai Tengfei Zhu Haohao Wu Yijie Wang Shiping Yang Xuefeng Li Junhong Zhuang Limei Chen Zhizhong Gong Feng Qin 2022Molecular Plant2022,15,10:2
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