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| 1 | NMR and LC/MS‐based global metabolomics to identify serum biomarkers differentiating hepatocellular carcinoma from liver cirrhosis显示文摘 | Yue Liu Zhanying Hong Guangguo Tan Xin Dong Genjin Yang Liang Zhao Xiaofei Chen Zhenyu Zhu Ziyang Lou Baohua Qian Guoqing Zhang Yifeng Chai | 2014 | Int. J. Cancer2014，，3： | 1 |
| 2 | In situ synthesis and unidirectional insertion of membrane proteins in liposome-immobilized silica stationary phase for rapid preparation of microaffinity chromatography显示文摘Cell membrane affinity chromatography has been widely applied in membrane protein(MP)-targeted drug screening and interaction analysis.However,in current methods,the MP sources are derived from cell lines or recombinant protein expression,which are time-consuming for cell culture or purification,and also difficult to ensure the purity and consistent orientation of MPs in the chromatographic stationary phase.In this study,a novel in situ synthesis membrane protein affinity chromatography(iSMAC)method was developed utilizing cell-free protein expression(CFE)and covalent immobilized affinity chromatography,which achieved efficient in situ synthesis and unidirectional insertion of MPs into liposomes in the stationary phase.The advantages of iSMAC are:1)There is no need to culture cells or prepare recombinant proteins;2)Specific and purified MPs with stable and controllable content can be obtained within 2 h;3)MPs maintain the transmembrane structure and a consistent orientation in the chromatographic stationary phase;4)The flexible and personalized construction of cDNAs makes it possible to analyze drug binding sites.iSMAC was successfully applied to screen PDGFRβinhibitors from Salvia miltiorrhiza and Schisandra chinensis.Micro columns prepared by in-situ synthesis maintain satisfactory analysis activity within 72 h.Two new PDGFRβinhibitors,salvianolic acid B and gomisin D,were screened out with KD values of 13.44 and 7.39μmol/L,respectively.In vitro experiments confirmed that the two compounds decreasedα-SMA and collagen Ⅰ mRNA levels raised by TGF-βin HSC-T6 cells through regulating the phosphorylation of p38,AKT and ERK.In vivo,Sal B could also attenuate CCl_(4)-induced liver fibrosis by downregulating PDGFRβdownstream related protein levels.The iSMAC method can be applied to other general MPs,and provides a practical approach for the rapid preparation of MP-immobilized or other biological solid-phase materials. | Yanqiu Gu Rong Wang Panpan Chen Shengnan Li Xinyi Chai Chun Chen Yue Liu Yan Cao Diya Lv Zhanying Hong Zhenyu Zhu Yifeng Chai Yongfang Yuan Xiaofei Chen | 2022 | Acta Pharmaceutica Sinica B2022，12，9： | 1 |
| 3 | The metabolism and N-oxide reduction of olaquindox in liver preparations of rats, pigs and chicken显示文摘 | LIU Zhanying HUANG Lingli CHEN Dongmei | 2010 | Toxicol Lett2010，195，1： | 1 |
| 4 | Effect of air distribution on aerodynamic field and coal com- bustion in an arch-fired furnace显示文摘 | LIU Ruwei HUI Shien YU Zhanying etal | 2010 | Energy & Fuels2010，24，10： | 1 |
| 5 | Screening of immune cell activators from Astragali Radix using a comprehensive two-dimensional NK-92MI cell membrane chromatography/C18 column/time-of-flight mass spectrometry system显示文摘Astragali Radix(AR)is a clinically used herbal medicine with multiple immunomodulatory activities that can strengthen the activity and cytotoxicity of natural killer(NK)cells.However,owing to the complexity of its composition,the specific active ingredients in AR that act on NK cells are not clear yet.Cell membrane chromatography(CMC)is mainly used to screen the active ingredients in a complex system of herbal medicines.In this study,a new comprehensive two-dimensional(2D)NK-92MI CMC/C18 column/time-of-flight mass spectrometry(TOFMS)system was established to screen for potential NK cell activators.To obtain a higher column efficiency,3-mercaptopropyltrimethoxysilane-modified silica was synthesized to prepare the NK-92MI CMC column.In total,nine components in AR were screened from this system,which could be washed out from the NK-92MI/CMC column after 10 min,and they showed good affinity for NK-92MI/CMC column.Two representative active compounds of AR,isoastragaloside Ⅰ and astragaloside IV,promoted the killing effect of NK cells on K562 cells in a dose-dependent manner.It can thus suggest that isoastragaloside Ⅰ and astragaloside Ⅳ are the main immunomodulatory components of AR.This comprehensive 2D NK-92MI CMC analytical system is a practical method for screening immune cell activators from other herbal medicines with immunomodulatory effects. | Xinyi Chai Yanqiu Gu Lei Lv Chun Chen Fei Feng Yan Cao Yue Liu Zhenyu Zhu Zhanying Hong Yifeng Chai Xiaofei Chen | 2022 | Journal of Pharmaceutical Analysis2022，12，5： | 0 |
| 6 | Screening potential P-glycoprotein inhibitors by combination of a detergent-free membrane protein extraction with surface plasmon resonance biosensor显示文摘P-glycoprotein(P-gp)highly expressed in cancer cells can lead to multidrug resistance(MDR)and the combination of anti-cancer drugs with P-gp inhibitor has been a promising strategy to reverse MDR in cancer treatment.In this study,we established a label-free and detergent-free system combining surface plasmon resonance(SPR)biosensor with styrene maleic acid(SMA)polymer membrane proteins(MPs)stabilization technology to screen potential P-gp inhibitors.First,P-gp was extracted from MCF-7/ADR cells using SMA polymer to form SMA liposomes(SMALPs).Following that,SMALPs were immobilized on an SPR biosensor chip to establish a P-gp inhibitor screening system,and the affinity between P-gp and small molecule ligand was determined.The methodological investigation proved that the screening system had good specificity and stability.Nine P-gp ligands were screened out from 50 natural products,and their affinity constants with P-gp were also determined.The in vitro cell verification experiments demonstrated that tetrandrine,fangchinoline,praeruptorin B,neobaicalein,and icariin could significantly increase the sensitivity of MCF-7/ADR cells to Adriamycin(Adr).Moreover,tetrandrine,praeruptorin B,and neobaicalein could reverse MDR in MCF-7/ADR cells by inhibiting the function of P-gp.This is the first time that SMALPs-based stabilization strategy was applied to SPR analysis system.SMA polymer can retain P-gp in the environment of natural lipid bilayer and thus maintain the correct conformation and physiological functions of P-gp.The developed system can quickly and accurately screen small molecule ligands of complex MPs and obtain affinity between complex MPs and small molecule ligands without protein purification. | Yuhong Cao Jiahao Fang Yiwei Shi Hui Wang Xiaofei Chen Yue Liu Zhenyu Zhu Yan Cao Zhanying Hong Yifeng Chai | 2022 | Acta Pharmaceutica Sinica B2022，12，7： | 0 |
| 7 | The Design and Realization of Net Testing System on Campus Network显示文摘 | Zhanying Ren Shijie Liu | 2005 | 美中教育评论2005，2，11： | 0 |
| 8 | Combined genome-wide association studies and expression quantitative trait locus analysis uncovers a genetic regulatory network of floral organ number in a tree peony (Paeonia suffruticosa Andrews) breeding population显示文摘Great progress has been made in our understanding of floral organ identity determination and its regulatory network in many species;however,the quantitative genetic basis of floral organ number variation is far less well understood for species-specific traits from the perspective of population variation.Here,using a tree peony(Paeonia suffruticosa Andrews,Paeoniaceae)cultivar population as a model,the phenotypic polymorphism and genetic variation based on genome-wide association studies(GWAS)and expression quantitative trait locus(eQTL)analysis were analyzed.Based on 24 phenotypic traits of 271 representative cultivars,the transcript profiles of 119 cultivars were obtained,which indicated abundant genetic variation in tree peony.In total,86 GWAS-related cis-eQTLs and 3188 trans-eQTL gene pairs were found to be associated with the numbers of petals,stamens,and carpels.In addition,19 floral organ number-related hub genes with 121 cis-eQTLs were obtained by weighted gene co-expression network analysis,among which five hub genes belonging to the ABCE genes of the MADS-box family and their spatial–temporal co-expression and regulatory network were constructed.These results not only help our understanding of the genetic basis of floral organ number variation during domestication,but also pave the way to studying the quantitative genetics and evolution of flower organ number and their regulatory network within populations. | Liping Peng Yang Li Wanqing Tan Shangwei Wu Qing Hao Ningning Tong Zhanying Wang Zheng’an Liu Qingyan Shu | 2023 | Horticulture Research2023，10，7： | 0 |
| 9 | A Catalytic Copper/Cobalt Oxide Interface for Efficient Hydrogen Generation显示文摘Metal nanoparticles and metal oxides promisingly provide different catalytic active sites at their interfaces.Constructing high-density interfaces is essential to maximize synergies.Herein,a Cu-Co_(3)O_(4) nanoparticles interfacial structure produced via pyrolysis and moderate oxidation from metal-organic frameworks has been designed to boost the intrinsic activity.The Cu-Co_(3)O_(4) nanoparticles composites exhibit a turnover frequency of 57.5 min−1 for ammonia borane hydrolysis,far higher than those of monometallic Cu and Co_(3)O_(4) nanoparticles,showing the synergistic effect of Cu and Co_(3)O_(4) nanoparticles at their interface.Density functional theory calculations and in situ Raman spectroscopy reveal the catalytic mechanism of dual active sites,in which Co_(3)O_(4) nanoparticles at Cu-Co_(3)O_(4) interface efficiently bind and activate water molecules and Cu nanoparticles easily activate NH3BH3 molecules.This study opens up a new pathway for achieving high-efficiency noble metal-free catalysts for hydrogen generation and other heterogeneous catalysis. | Wenjing Xu Sheli Zhang Ruofan Shen Zhikun Peng Baozhong Liu Jun Li Zhanying Zhang Baojun Li | 2023 | Energy & Environmental Materials2023，6，2： | 0 |
| 10 | Optimal Design of Deforming Regulation for Compact Continuous Rolling Mill with Balanced Load显示文摘OptimalDesignofDeformingRegulationforCompactContinuousRolingMilwithBalancedLoadLiuZhanying①,QinGuoqing②ABSTRACTIfthedraughtof... | Liu Zhanying Qin Guoqing | 1997 | Journal of Iron and Steel Research(International)1997，4，2： | 0 |
| 11 | 胸腺五肽局部注射联合液氮冷冻治疗多发性跖疣的疗效观察显示文摘目的 :观察胸腺五肽局部注射联合液氮冷冻治疗多发性跖疣的临床疗效。方法:门诊选择120名多发性跖疣患者,随机分为2组,治疗组予胸腺五肽注射液疣体基底部局部注射治疗,每3天一次,3次后予液氮冷冻治疗;对照组仅予液氮冷冻治疗。结果:治疗组有效率90.3%,对照组有效率66.7%,两组间差异有显著性;治疗组复发率11.7%,对照组复发率33.3%,两组间差异有显著性。结论 :胸腺五肽注射液局部注射联合液氮冷冻治疗多发性跖疣疗效确切,复发率低。 | 时盼 HOU Zhanying HUANG Zhen LIU Fang LIU Wanghong | 2015 | 人人健康2015，，21： | 0 |
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