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| 1 | Two haplotype-resolved,gap-free genome assemblies for Actinidia latifolia and Actinidia chinensis shed light on the regulatory mechanisms of vitamin C and sucrose metabolism in kiwifruit显示文摘Kiwifruit is a recently domesticated horticultural fruit crop with substantial economic and nutritional value,especially because of the high content of vitamin C in its fruit.In this study,we de novo assembled two telomere-to-telomere kiwifruit genomes from Actinidia chinensis var.‘Donghong’(DH)and Actinidia latifolia‘Kuoye’(KY),with total lengths of 608327852 and 640561626 bp for 29 chromosomes,respectively.With a burst of structural variants involving inversion,translocations,and duplications within 8.39 million years,the metabolite content of DH and KY exhibited differences in saccharides,lignans,and vitamins.A regulatory ERF098 transcription factor family has expanded in KY and Actinidia eriantha,both of which have ultra-high vitamin C content.With each assembly phased into two complete haplotypes,we identified allelic variations between two sets of haplotypes,leading to protein sequence variations in 26494 and 27773 gene loci and allele-specific expression of 4687 and 12238 homozygous gene pairs.Synchronized metabolome and transcriptome changes during DH fruit development revealed the same dynamic patterns in expression levels and metabolite contents;free fatty acids and flavonols accumulated in the early stages,but sugar substances and amino acids accumulated in the late stages.The AcSWEET9b gene that exhibits allelic dominance was further identified to positively correlate with high sucrose content in fruit.Compared with wild varieties and other Actinidia species,AcSWEET9b promoters were selected in red-flesh kiwifruits that have increased fruit sucrose content,providing a possible explanation on why red-flesh kiwifruits are sweeter.Collectively,these two gap-free kiwifruit genomes provide a valuable genetic resource for investigating domestication mechanisms and genome-based breeding of kiwifruit. | Xue Han Yilin Zhang Qiong Zhang Ni Ma Xiaoying Liu Wenjing Tao Zhiying Lou Caihong Zhong Xing Wang Deng Dawei Li Hang He | 2023 | Molecular Plant2023，16，2： | 3 |
| 2 | Microbiologically influenced corrosion of 304 stainless steel by halophilic archaea Natronorubrum tibetense显示文摘The corrosion behavior of 304 stainless steel(SS)in the presence of aerobic halophilic archaea Natronorubrum tibetense was investigated.After 14 days of immersion,no obvious pitting pit was observed on the SS surface in the sterile medium.By contrast,the SS exhibited serious pitting corrosion with the largest pit depth of 5.0μm in the inoculated medium after 14 days.The results of electrochemical tests showed that the barrier property of the passive film decreased faster in the inoculated medium.The X-ray photoelectron spectroscopy results indicated that the detrimental Fe2+and Cr6+increased in the passive film under the influence of archaea N.tibetense,which resulted in the accelerated deterioration of passive film and promoted the pitting corrosion.Combined with the energy starvation tests,the microbiologically influenced corrosion mechanism of 304 SS caused by halophilic archaea N.tibetense was discussed finally. | Hongchang Qian Lingwei Ma Dawei Zhang Ziyu Li Luyao Huang Yuntian Lou Cuiwei Du | 2020 | Journal of Materials Science & Technology2020，42，11： | 3 |
| 3 | Extraction of formic and acetic acids from aqueous solution by dy- namic headspace-needle trap extraction: Temperature and pH optimization显示文摘 | Lou Dawei Lee Xinqing Pawliszyn J | 2008 | J Chromatogr A2008，1201，2： | 1 |
| 4 | Mechanically durable antibacterial nanocoatings based on zwitterionic copolymers containing dopamine segments显示文摘Developing an effective and durable antibacterial surface is important for surgical tools and biomedical implants.In this work,a zwitterionic copolymer containing catechol groups as biomimetic anchoring segments was coated onto 316 L stainless steel via drop-casting.Energy-dispersive X-ray spectroscopy(EDS)and water contact angle(WCA)measurements indicated that the coatings made of the copolymers containing zwitterionic and dopamine segments at the molar ratios of 8:2 and 6:4 exhibited stronger stability and mechanical durability than the one at 9:1 after inducing tape-peeling and ultrasonication damage.The mechanically durable nanocoatings exhibited excellent antibacterial performance against Staphylococcus aureus and Escherichia coli in a period of 3 days.The nanocoatings with zwitterionic and dopamine segments at the molar ratio of 8:2 were further evaluated and demonstrated durable antibacterial performance after tape-peeling and ultrasonication treatments. | Jingzhi Yang Hongchang Qian Junpeng Wang Pengfei Ju Yuntian Lou Guoliang Li Dawei Zhang | 2021 | Journal of Materials Science & Technology2021，，30： | 1 |
| 5 | T2 relaxation time measurements in the brains of scalded rats显示文摘This study aimed to evaluate the T2 relaxation time of the brain in severely scalded rats using a magnetic resonance(MR) T2 mapping sequence,and to investigate the correlation between T2 relaxation time and plasma glucose level.Twenty-eight Wistar rats were randomly divided into the scalded group(n=21)and control group(n=7).Magnetic resonance scans were performed with TIWI,T2 WI,and T2-mapping sequences in the scalded group;the scans were performed 1 day prior to scalding and 1,3,5,and7 days post-scalding;in addition,identical MR scans were performed in the control group at the same time points.T2-maps were generated and T2 relaxation times were acquired from the following brain regions:the hippocampus,thalamus,caudate-putamen,and cerebrum.Pathological changes of the hippocampus were observed.The plasma glucose level of each rat was measured before each MR scan,and a correlation analysis was performed between T2 relaxation time and plasma glucose level.We found that conventional TIWI and T2 WI did not reveal any abnormal signals or morphological changes in the hippocampus,thalamus,caudate-putamen,or cerebrum post-scalding.Both the T2 relaxation times of the selected brain regions and plasma glucose levels increased 1,3,and 5 days post-scalding,and returned to normal levels 7 days post-scalding.The most marked increase of T2 relaxation time was found in the hippocampus;similar changes were also revealed in the thalamus,caudate-putamen,and cerebrum.No correlation was found between T2 relaxation time and plasma glucose level in scalded rats.Pathological observation of the hippocampus showed edema 1,3,and 5 days post-scalding,with recovery to normal findings at 7 days post-scalding.Thus,we concluded that T2 mapping is a sensitive method for detecting and monitoring scald injury in the rat brain.As the hippocampus is the main region for modulating a stress reaction,it showed significantly increased water content along with an increased plasma glucose level post-scalding. | Tao Jiang Liqi Xie Xin Lou Dawei Li Zhiye Chen Huafeng Xiao Lin Ma | 2017 | Science China(Life Sciences)2017，60，1： | 1 |
| 6 | Application of Fuzzy- PID Controller in Heating Ventilating and Air-Conditioning System 显示文摘 | Jiangjiang Wang Dawei An Chengzhi Lou | 2006 | Proceedings of the Mechatronics and Automation2006，，7： | 1 |
| 7 | Investigation on occupant presence and appliance operation schedules for university campus in south China sub-tropical area显示文摘Building occupant presence during varying periods is crucial to the performance studies of buildings and city regions.However,the understanding of the building occupancies on the university campus remains limited.To address this gap,our study employs field measurements,payment records,course arrangements,and building access systems to depict the occupancy patterns of the canteen,dormitory,library,and teaching and lab buildings during weekdays and weekends.We found that the occupancy rates across different buildings are somehow interrelated,given that the total number of occupants on campus is generally constant.Notably,dormitory occupancy rates tend to be low during the morning and afternoon course hours,which inversely correlates with the high occupancy rates in the teaching and lab buildings during these periods.Similarly,canteens experience surges in occupancy during meal times,which coincide with a decrease in library usage.Moreover,we established appliance operation schedules for dormitories through surveys and on-site investigations.Water dispensers and electronic devices were identified as the primary energy consumers for both male and female occupants,with desk-top fans and hairdryers being significant energy users for male and female occupants,respectively.These findings are essential for energy studies within a campus setting,underlining the importance of considering occupant behaviors on a regional scale. | Siwei Lou Zhongyuan Lin Yukai Zou Dawei Xia Yu Huang Zhuohong Li Zhaowen Gu | 2024 | Building Simulation2024，17，2： | 0 |
| 8 | Development of Gelatin-Based Active Packaging and Its Application in Bread Preservation显示文摘The issue of plastic pollution has attracted widespread social attention.Gelatin is valued as a degradable bio-based material,especially as an edible active packaging material.However,the commonly used solution-casting filmforming technology limits the mass production of gelatin films.In order to improve the production efficiency and enhance the commercial value of gelatin films,in this study,fish gelatin(FG)particles were successfully blended with essential oils(EOs)to prepare active films by melt extrusion technique,a common method for commercial plastics,and applied to bread preservation.FG and EOs showed good compatibility with each other.The elongation at break was enhanced in all samples of films containing EOs.The addition of EOs weakened the oxygen barrier properties of the FG films.The gelatin films containing clove EO showed the highest DPPH radical scavenging rate of 39.38%.The films containing oregano EO showed the highest antibacterial activity,with 98.84%and 99.86% against S.aureus and E.coli,respectively.The bread preservation results showed a slower microbial growth rate in the samples preserved by the active films.The bread samples preserved in commercial PE film showed mold on day 3,and the bread samples preserved in active gelatin film showed mold visible to the naked eye by day 7.This study demonstrates the potential of gelatin-based activated films for commercial application in baked goods preservation. | Hui Zheng Xiaohan Chen Li Li Dawei Qi Jiale Wang Jiaying Lou Wenjun Wang | 2023 | Journal of Renewable Materials2023，11，10： | 0 |
| 9 | Microbiologically Influenced Corrosion Behavior of Fe_(40)(CoCrMnNi)_(60) and Fe_(60)(CoCrMnNi)_(40) Medium Entropy Alloys in the Presence of Pseudomonas Aeruginosa显示文摘In this work,the microbiologically influenced corrosion(MIC)of Fe_(40)(CoCrMnNi)_(60) and Fe_(60)(CoCrMnNi)_(40) medium entropy alloys(MEAs)induced by Pseudomonas aeruginosa(P.aeruginosa)was investigated.Corrosion behaviors during 14 days of immersion in sterile and P.aeruginosa-inoculated culture media are presented.Under sterile conditions,both MEAs exhibited good corrosion resistance against the culture medium solution.In the presence of P.aeruginosa,the pitting corrosion of MEAs was promoted.The results of inductively coupled plasma‒mass spectrometry(ICP‒MS)and potentiodynamic polarization tests showed that the presence of P.aeruginosa promoted the selective dissolution of passive film and accelerated the corrosion of MEAs.The results of X-ray photoelectron spectroscopy(XPS)and Mott-Schottky measurements further demonstrated the degradation effect of P.aeruginosa on the passive film.Compared with Fe_(60)(CoCrMnNi)_(40),Fe_(40)(CoCrMnNi)_(60) manifested better resistance to the MIC caused by P.aeruginosa,which may be attributed to more Cr oxides and fewer Fe oxides of the passive film. | Weiwei Chang Yangyang Li Huaibei Zheng Hongchang Qian Dawei Guo Shuyuan Zhang Yuntian Lou Chi Tat Kwok Lap Mou Tam Dawei Zhang | 2023 | Acta Metallurgica Sinica(English Letters)2023，36，3： | 0 |
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