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| 1 | Nitrite-derived nitric oxide by xanthine oxidoreductase protects the liver against ischemia- reperfusion injury显示文摘It was demonstrated that xanthine oxidoreductase (XOR), during ischemia, catalyzes the formation of nitric oxide (NO) from nitrite (NO2-) and this NO2--derived NO protects the isolated perfused rat heart against the damaging effects of ischemia-reperfusion (I/R) when conventional nitric oxide synthase (NOS) -dependent NO production is impaired. Liver is one of the organs with the highest XOR concentration. This study was designed to determine whether NO2--derived NO by XOR protects liver against I/R injury in vivo. For its minute amounts and active reactivity, NO can not be detected directly in real time in vivo by this time. We have to prove the above hypothesis indirectly. METHODS:Wistar rats were pretreated with saline, NOS inhibitor L-NAME (10 mg/kg intravenously), XOR inhibitor allopurinol (1.5 mg/kg orally), L-NAME +allopurinol and NO scavenger carboxy-PTIO (0.6 mg/kg intravenously) respectively (12 animals per group). And then, they were subjected to total liver ischemia for 40 minutes followed by reperfusion. Blood samples and liver tissues were obtained for analysis after 3 hours of reperfusion. Survival was also investigated. RESULTS:Allopurinol-treated animals exhibited further increased serum alanine aminotransferase (ALT) levels and liver myeloperoxidase (MPO) activities, but further decreased liver adenosine triphosphate (ATP) stores after I/R compared to saline-treated counterparts (830.5±108.3 U/L, 56.5±11.0 U/mg protein and 1.93±0.47 μmol/g vs. 505.8± 184.2 U/L, 41.5±10.2 U/mg protein and 3.05±0.55 μmol/g respectively, P<0.01, P<0.05 and P<0.01 respectively). The hepatocyte injury was further exacerbated and the overall survival rate was significantly decreased after I/R in animals given by allopurinol compared to those pretreated by saline (P<0.05). L-NAME and allopurinol co-treated animals exhibited more severe liver injury (P<0.05 and P<0.01) and a further decreased overall survival rate (P<0.05) compared to L-NAME or allopurinol alone-treated counterparts, but they were not different from carboxy-PTIO treated animals (P>0.05). CONCLUSION:NO2--derived NO by XOR in the hypoxic and acidic environment induced by hepatic I/R protects the liver against I/R injury in vivo. | Department of General Surgery ( Lu P, Wang CY and Chen DD), and Department of Radiology (Liu F), Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China Cold Spring Harbor Laboratory, New York 11724, USA ( Yao Z) General Surgery Laboratory, Union Hospital, Tongji Medical College , Huazhong University of Science and Technology, Wuhan 430022 , China (Tian Y, Zhang JH and Wu YH) | 2005 | Hepatobiliary & Pancreatic Diseases International2005，4，3： | 4 |
| 2 | Changing trends of surgical treatment of hilar bile duct cancer:clinical and experimental perspectives显示文摘HISTORICAL POINT OF VIEWCarcinoma of the confluence of the hepatic ductshas been thought to be a rare disease until recently.Carcinoma of the large hepatic ducts was rarelydiagnosed correctly premorterned.Because of itsdeeply seated location,resection was once thoughtto be impossible.In 1957,Altemeier reported | Huang ZQ Zhou NX Wang DD Lu JG Chen MY | 2000 | World Journal of Gastroenterology2000，6，6： | 2 |
| 3 | An oral selective M3cholinergic receptor antagonist in COPD显示文摘 | Lu S Parekh DD Kuznetsova O | 2006 | Eur Respir J2006，28，4： | 1 |
| 4 | Layered Li Ozcathode materials for lithium- ion Batteries 显示文摘 | Lu ZH MaeNeil DD Dahn JR | 2001 | Electrochem Solid State Lett2001，4，12： | 1 |
| 5 | Real-time reverse transcription-PCR assay panel for Middle East respiratory syndrome coronavirus 显示文摘 | Lu X Whitaker B Sakthivel SK Kamili S Rose LE Lowe L Mohareb E Elassal EM Al-sanouri T Haddadin A Erdman DD | 2014 | J Clin Microbiol2014，52，1： | 1 |
| 6 | Expression and significance of new tumor suppressor gene PTEN in primary liver cancer显示文摘 | Lu DD Zhang XR Cao XR | 2003 | J Cell Mol Med2003，7，1： | 1 |
| 7 | Polyphyllin D exerts potent anti-tumoureffects on lewis cancer cells under hypoxic conditions显示文摘 | Ma DD Lu HX Xu IS | 2009 | J Int Med Res2009，37，3： | 1 |
| 8 | Specialization of Phonological and Semantic Processing in Chinese Word Reading显示文摘 | BOOTH JR DONG LU BURMAN DD | 2006 | Brain Research(S0079-6123)2006，1071，： | 1 |
| 9 | The relationship between metal- lothionein-lF (MT1F) gene and hepatocellular carcinoma显示文摘 | Lu DD Chen YC Zhang XR | 2003 | Yale J Biol Meal2003，76，2： | 1 |
| 10 | Sample classifcation of GC-ToF-MS metabolomics data without the requirement for chromatographicdeconvolution显示文摘 | Lu HM Gan DD Zhang ZM | 2011 | Metabolomics2011，7，2： | 1 |
| 11 | Rhinovirus-associated hospitali- zations in young children 显示文摘 | Miller EK Lu X Erdman DD | 2007 | J Infect Dis2007，195，6： | 1 |
| 12 | Structure and electro- chemistry of Li 02 显示文摘 | MacNeil DD Lu Z Dahn JR | 2002 | J Electrochem- Soc2002，149，： | 1 |
| 13 | Quantitative real- time PCR assay panel for detection and type-specific idenfication of peidemic respiratory human adenovirus 显示文摘 | Lu X Trujillo-Lopez E Lott Erdman DD | 2012 | J Clin Microbiol2012，51，4： | 1 |
| 14 | REST and its corepressors mediate plasticity of neuronal gene chromatin throughout neurogenesis 显示文摘 | Ballas N Grunseich C Lu DD | 2005 | Cell2005，121，4： | 1 |
| 15 | Cationic liposomemediated GDNF gene transfer after spinal cord injury显示文摘 | Lu KW Chen ZY Jin DD | 2002 | J Neurotrauma2002，19，9： | 1 |
| 16 | Specialization of phonological and semantic processing in Chinese word reading显示文摘 | Booth JR Lu D Bunnan DD | 2006 | Brain Res2006，1071，： | 1 |
| 17 | Rhinovirus-associated hospitalizations in young children显示文摘 | Miller EK Lu X Erdman DD Poehling KA Zhu Y Griffin MR | | 0，，06： | 1 |
| 18 | Mean platelet volume and D-dimer in patients with suspected deep venous thrombosis显示文摘 | Arzu Canan S?dd?ka Sezgin Hal?c?o?lu Safiye Gürel | 2012 | Journal of Thrombosis and Thrombolysis2012，，2： | 1 |
| 19 | REST and its compressors mediate plasticity of neuronal gene chromatin throughout neuregenesis显示文摘 | Grunseich C Lu DD | 2005 | Cell2005，121，： | 1 |
| 20 | Rhinovirus-associated hospitalizations in young children显示文摘 | Miller EK Lu X Erdman DD | | 0，，06： | 1 |
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