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| 1 | Analysis of cytochrome P450 genes in silkworm genome (Bombyx mori)显示文摘We have searched the Bombyx mori genome for members of the major enzyme family, the Cytochrome P450s, which carry out multiple reactions to enable organisms to rid themselves of foreign compounds. As a result, 86 putative P450s were discovered in silkworm genome, which are thought to belong to 32 subfamilies. A comparative genomic analysis with Drosophila melanogaster reveals that the two insects have some similar P450 distribution pat-terns but still have some obvious differences. Especially, the diverse distribution exists in 7 p450 subfamilies, which are CYP4A, CYP4C, CYP4D, CYP6A, CYP6AE, CYP6B and CYP9A. Fur-thermore, we collected expression sequence tag (EST) evidence for 49 putative P450s genes, which are expressed at the transcriptional level and more likely to be true P450s. | LI Bin XIA Qingyou LU Cheng ZHOU Zeyang XIANG Zhonghuai | 2005 | Science China(Life Sciences)2005，48，4： | 7 |
| 2 | Understanding divergent domestication traits from the whole-genome sequencing of swamp-and river-buffalo populations显示文摘Domesticated buffaloes have been integral to rice-paddy agro-ecosystems formillennia,yet relatively little is known about the buffalo genomics.Here,we sequenced and assembled reference genomes for both swamp and river buffaloes and we re-sequenced 230 individuals(132 swamp buffaloes and 98 river buffaloes)sampled from across Asia and Europe.Beyond the many actionable insights that our study revealed about the domestication,basic physiology and breeding of buffalo,we made the striking discovery that the divergent domestication traits between swamp and river buffaloes can be explained with recent selections of genes on social behavior,digestion metabolism,strengths and milk production. | Xier Luo Yu Zhou Bing Zhang Yi Zhang Xiaobo Wang Tong Feng Zhipeng Li Kuiqing Cui Zhiqiang Wang Chan Luo Hui Li Yanfei Deng Fenghua Lu Jianlin Han Yongwang Miao Huaming Mao Xiaoyan Yi Cheng Ai Shigang Wu Alun Li Zhichao Wu Zijun Zhuo Do Da Giang Bikash Mitra Mohammad Farhad Vahidi Shahid Mansoor Sahar Ahmed Al-Bayatti Eka Meutia Sari Neena Amatya Gorkhali Sigit Prastowo Laiba Shafique Guoyou Ye Qian Qian Baoshan Chen Deshun Shi Jue Ruan Qingyou Liu | 2020 | National Science Review2020，7，3： | 3 |
| 3 | Atomic resolution scanning tunneling microscope imaging up to 27 T in a water-cooled magnet显示文摘我们在一个 water-cooled 磁铁(WM ) 报导第一原子地解决的扫描通道显微镜(STM ) 成像,为哪个极其严厉的颤动和噪音是主要挑战。这特定的 WM-STM 展示粗糙的途径被我们的最新设计的 TunaDrive 压电的马达在驾驶的一个极端僵硬、紧缩的扫描头。挂系统的一个三水平的弹簧被用于颤动隔离。有优秀原子分辨率的石墨的房间温度未加工数据的图象面对很强壮的磁场被获得,与直到 27 T 的领域力量,在有在 10 MW 的一个力量等级的 27.5 T 的最大的领域力量的 32-mm-diameter 讨厌的人 WM ,由原子磁性的回声( NMR )校准了。27 T 的这记录地力量超过常规超导的磁铁完成的最大的地力量。而且,我们的 WM-STM 用 45 T 铺平了道路到 STM 成像, 32-mm-diameter 讨厌的人混血儿磁铁,是世界旗舰磁铁,生产最强壮的稳定的磁场。 | Wenjie Meng Ying Guo Yubin Hou Qingyou Lu | 2015 | Nano Research2015，8，12： | 2 |
| 4 | A Draft Sequence for the Genome of the Domesticated Silkworm (Bombyx mori)显示文摘 | Xia Qingyou Zhou Zeyang Lu Cheng | 2004 | Science2004，306，： | 1 |
| 5 | A draft sequence for the genome of the domesticated silkworm (Bombyx mori)显示文摘 | Xia Qingyou Zhou Zeyang Lu Cheng | 2004 | Science2004，306，： | 1 |
| 6 | A draft sequence for the genome of the domesticated si]k worm( bombyxmori) 显示文摘 | Qingyou Xia Zeyang Zhou Cheng Lu | 2004 | Science2004，306，5703： | 1 |
| 7 | A draft sequence for the genome of the domesticated silkworm ( bombyx mori) 显示文摘 | Xia Qingyou Zhou Zeyang Lu Cheng etal | 2004 | Science2004，306，： | 1 |
| 8 | A draft sequence for the genome of the domesticated silkworm (Bombyx mari)显示文摘 | XIA QINGYOU ZHOU ZEYANG LU CHENG | 2004 | Science2004，，306： | 1 |
| 9 | A draft sequence for the genome of the domesticated silkworm (Bombyx mori)显示文摘 | Qingyou Xia Zeyang Zhou Cheng Lu | 2004 | Science2004，，306： | 1 |
| 10 | Protective effect of Morinda officinalis polysaccharides on bone degeneration in the aged rats 显示文摘 | Wang ZongBao Lu QingYou Lu HaiYan | 2011 | International Journal of the Physical Sciences2011，6，1： | 1 |
| 11 | A Draft Sequence for the Genome of the Domesticated Silkworm显示文摘 | Qingyou Xia Zeyang Zhou Cheng Lu etal | 2004 | SCIENCE2004，306，： | 1 |
| 12 | Developing an efficient and visible prime editing system to restore tobacco 8-hydroxy-copalyl diphosphate gene for labdane diterpene Z-abienol biosynthesis显示文摘Prime editing(PE)is a versatile CRISPR-Cas based precise genome-editing platform widely used to introduce a range of possible base conversions in various organisms.However,no PE systems have been shown to induce heritable mutations in tobacco,nor in any other dicot.In this study,we generated an efficient PE system in tobacco that not only introduced heritable mutations,but also enabled anthocyanin-based reporter selection of transgene-free T_(1) plants.This system was used to confer Zabienol biosynthesis in the allotetraploid tobacco cultivar HHDJY by restoring a G>T conversion in the NtCPS2 gene.High levels of Z-abienol were detected in the leaves of homozygous T_(1) plants at two weeks after topping.This study describes an advance in PE systems and expands genome-editing toolbox in tobacco,even in dicots,for use in basic research and molecular breeding.And restoring biosynthesis of Z-abienol in tobacco might provide an efficient way to obtain Z-abienol in plants. | Jianduo Zhang Lu Zhang Chengwei Zhang Yongxing Yang Huayin Liu Lu Li Shengxue Zhang Xianggan Li Xinxiang Liu Ya Liu Jin Wang Guangyu Yang Qingyou Xia Weiguang Wang Jinxiao Yang | 2023 | Science China(Life Sciences)2023，66，12： | 1 |
| 13 | A draft sequence for the genome of the domesticated silkworm (Bombyx mori) 显示文摘 | Qingyou Xia Zeyang Zhou Cheng Lu | 2004 | Science2004，306，： | 1 |
| 14 | Tissue-specific genome editing of laminA/C in the posterior silk glands of Bombyx mori显示文摘The RNA-guided CRISPR/Cas9 system has been shown to be a powerful tool for genome editing in various organisms. A comprehensive toolbox for multiplex genome editing has been developed for the silkworm,Bombyx mori, a lepidopteran model insect of economic importance. However, as previous methods mainly relied on delivery of transient Cas9/guide RNA(gRNA), they could not be used in loss-of-function studies of essential genes. Here, we report a simple and versatile tissue-specific genome editing strategy.We perform a proof-of-principle demonstration by establishing and crossing two transgenic B. mori lines,one expressing Cas9 protein in the posterior silk glands(PSGs) and the other constitutively expressing BmlaminA/C(BmLMN) gRNA. All BmLMN alleles in the PSG cells were edited precisely at the target genome region, resulting in diverse mutations. mRNA expression of BmLMN was reduced by up to 75%,and only very low levels of BmLaminA/C protein were detected. Knockout of BmLMN produced obvious defects in gland cell development and cocoon production. In this study, we developed an efficient strategy for spatially controlled genome editing, providing unprecedented opportunities for investigating the function of essential/lethal genes in B. mori, with potential application for other insects. | Yuanyuan Liu Sanyuan Ma Jiasong Chang Tong Zhang Xiaogang Wang Run Shi Jianduo Zhang Wei Lu Yue Liu Qingyou Xia | 2017 | Journal of Genetics and Genomics2017，44，9： | 0 |
| 15 | Facile and Low-Cost Fabrication of a Thread/Paper-Based Wearable System for Simultaneous Detection of Lactate and pH in Human Sweat显示文摘Wearable devices have received tremendous interests in human sweat analysis in the past few years.However,the widely used polymeric substrates and the layer-by-layer stacking structures greatly influence the cost-efficiency,conformability and breathability of the devices,further hindering their practical applications.Herein,we report a facile and low-cost strategy for the fabrication of a skin-friendly thread/paper-based wearable system consisting of a sweat reservoir and a multi-sensing component for simultaneous in situ analysis of sweat pH and lactate.In the system,hydrophilic silk thread serves as the micro-channel to guide the liquid flow.Filter papers were functionalized to prepare colorimetric sensors for lactate and pH.The smartphone-based quantitative analysis shows that the sensors are sensitive and reliable.Although pH may interfere the lactate detection,the pH detected simultaneously could be employed to correct the measured data for the achievement of a precise lactate level.After being integrated with a hydrophobic arm guard,the system was successfully used for the on-body measurement of pH and lactate in the sweats secreted from the volunteers.This low-cost,easy-to-fabricate,light-weight and flexible thread/paper-based microfluidic sensing device may hold great potentials as a wearable system in human sweat analysis and point-of-care diagnostics. | Gang Xiao Jing He Yan Qiao Feng Wang Qingyou Xia Xin Wang Ling Yu Zhisong Lu Chang-Ming Li | 2020 | Advanced Fiber Materials2020，2，5： | 0 |
| 16 | Estimation of Enthalpy of Formation Using Benson’s Group Addition and Functional Group Correction显示文摘The enthalpies of formation of solid organic compounds containing carbon,nitrogen,oxygen,and hydrogen were estimated using two suggested descriptor sets,separately,by machine learning methods.The two descriptor sets are both composed of descriptors of Benson’s groups and corrected groups.The main differences between them are that one is generated based on atoms and the other is based on bonds.An in-house program was specially written in Java to extract all the descriptors with a function to ensure that each atom(or bond)of a molecule is represented by Benson’s groups once for an atom-based(or bond-based)descriptor set.Multiple linear regression and partial least squares were used,separately,to build models to predict the enthalpy of formation for two descriptor sets.The combination of the models constructed by two descriptor sets based on the atoms and the bonds achieved the best-predicted results in this paper,and the corresponding results of the test set are better than that in the literature,from which the original data were retrieved.Further,a small data set of fluorinated molecules was collected,and satisfactory results were also obtained for these molecules containing fluorine with the assistance of the former data set. | LI Xianlan LUAN Yue LU Yanhua LI Wei MA Lihong ZHANG Qingyou PANG Aimin | 2023 | Chemical Research in Chinese Universities2023，39，2： | 0 |
| 17 | SAA-Cas9: A tunable genome editing system with increased bio-safety and reduced off-target effects显示文摘The site-specific nature and ease of handling of clustered regularly interspaced palindromic repeat(CRISPR)/Cas9 has fulfilled the decades-long goal of genome engineering,and thus has pushed the biological sciences into a new era in which site-directed manipulation of DNA is no longer a short slab(Doudna and Charpentier,2014).The incomparable power of this system also enabled unprecedented new opportunities for practical applications in agriculture,ecosystem and human health(Liu,2017).However,with the extremely rapid development and application of CRISPR system,concerns about its bio-safety have been increasing(Caplan et al.,2015;Taning et al.,2017).While multiple studies have addressed this issue,alternative options for regulating Cas9 are of great interest.As has been proved by increasing evidences,another well?known concern is the off-target effects,which could not only confound research experiments or breeding,but also cause unwanted side effects in the forthcoming therapeutic uses(Fu et aL,2013;Taning et al.,2017;Zhang et al.,2018). | Sanyuan Ma Jianduo Zhang Wei Lu Yue Liu Qingyou Xia | 2019 | Journal of Genetics and Genomics2019，46，3： | 0 |
| 18 | Efficiently Preparing Silk Fibroin Nanofibers by Ultrasonication显示文摘Ultrasonication was used to prepare silk fibroin nanofibers directly from dumming silk fiber in aquous solution.The existing problems,the key influencing factors and different pretreated methods in ultrasonic preparation of SFN were analyzed detailly.The SFF pretreated with mixed solutions of sodium carbonate and sodium phosphate could | Jiang Yanke Ding Huan Lu Zhongyan Zhao Ping Xia Qingyou | 2016 | 功能材料信息2016，13，4： | 0 |
| 19 | Individual variation in buffalo somatic cell cloning efficiency is related to glycolytic metabolism显示文摘Mammalian individuals differ in their somatic cell cloning efficiency,but the mechanisms leading to this variation is poorly understood.Here we found that high cloning efficiency buffalo fetal fibroblasts(BFFs)displayed robust energy metabolism,looser chromatin structure,high H3 K9 acetylation and low heterochromatin protein 1α(HP1α)expression.High cloning efficiency BFFs had more H3 K9 ac regions near to the upstream of glycolysis genes by Ch IP-seq,and involved more openness loci related to glycolysis genes through ATAC-seq.The expression of these glycolysis genes was also found to be higher in high cloning efficiency BFFs by q RT-PCR.Two key enzymes of glycolysis,PDKs and LDH,were confirmed to be associated with histone acetylation and chromatin openness of BFFs.Treatment of low cloning efficiency BFFs with PS48(activator of PDK1)resulted in an increase in the intracellular lactate production and H3 K9 acetylation,decrease in histone deacetylase activity and HP1αexpression,less condensed chromatin structure and more cloning embryos developing to blastocysts.These results indicate that the cloning efficiency of buffalo somatic cells is associated with their glycolytic metabolism and chromatin structure,and can be improved by increasing glycolytic metabolism. | Chan Luo Zhiqiang Wang Jinling Wang Feng Yun Fenghua Lu Jiayuan Fu Qingyou Liu Deshun Shi | 2022 | Science China(Life Sciences)2022，65，10： | 0 |
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