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| 1 | Structure-efficacy relationships of immunostimulatory activity of CpG-containing oligodeoxynucleotides on mouse spleen cells显示文摘瞄准:学习在包含 unmethylated deoxycytidyl-deoxyguanosine (CpG ) 的 oligodeoxynucleo 潮(ODN ) 的主要结构之间的关系在老鼠怒气房间的二核甙酸主题和他们的 immunostimulatory 活动。方法:有不同主要结构的一系列 CpG ODN 被综合。他们刺激老鼠怒气房间增长的能力被决定由[3H ] 胸腺嘧啶核甙加入试金。Cytokine (interleukin [IL ]-6, IL-12,和 IFN-alpha ) CpG ODN 导致的分泌物系列被 ELISA 估计。到 activate 天赋杀手房间的 CpG ODN 的能力被标准 4 h (51 ) 评估 Cr 版本试金。流动血细胞计数被利用在多样的 immunocytes 上检验各种各样的淋巴细胞表面分子的表情。有效 CpG ODN 为鼠科, ODN1826,被放作为修正和积极控制的模板。结果:有不同序列和作文的 CpG ODN 的 immunostimulatory 活动显著地变化了,在特性并且在程度。它为由简单地增加功能的 hexameric CpG 主题数字改进 ODN1826 的 immunostimulatory 活动是无用的,修改 CpG 主题的地点,或改变在 multi-CpG 主题之间的距离。然而,自我补足的回文的增加在第 3'-e,然而并非第 5'-e CpG ODN 组织,在它的活动的兴奋显著改进。几设计 ODN 与 ODN1826 相比有优异全面 immunostimulatory 性质。结论:CpG ODN 的 immunostimulatory 活动与它的主要结构相关。简单地改变 CpG 主题数字,空格,或距离,为支持 immunostimulatory 活动是无用的。CpG ODN 的第 3'-e 回文结构与提高的 immunostimulatory 活动被联系。 | Hai-yan DU Shao-you XIA Hai-feng SONG Na LI Ming-mei SHANG Jia ZOU Qing-qing WANG Lun OU XiaoSUN Ai-guo JI Zhong-ming TANG | 2007 | Acta Pharmacologica Sinica2007，28，10： | 1 |
| 2 | Rehabilitation treatment of patients with injured anterior cruciate ligaments of knees 显示文摘 | Huang Xiao-hua Zhu Shao-ting Ou Lun | 2004 | Chin J Trad Med Traumatol Orthop2004，12，1： | 1 |
| 3 | Development and Validation of a Simoa Assay for Determination of Recombinant Batroxobin in Human Serum显示文摘Recombinant batroxobin(S3101)is a thrombin-like serine protease that binds to fibrinogen or is taken up by the reticuloendothelial system.A literature survey showed no adequate method that could determine sufficient concentrations to evaluate pharmacokinetic parameters for phase I clinical studies.Therefore,a sensitive method is urgently needed to support the clinical pharmacokinetic evaluation of S3101.In this study,a sensitive bioanalytical method was developed and validated,using a Quanterix single molecular array(Simoa)assay.Moreover,to thoroughly assess the platform,enzyme-linked immunosorbent assay and electrochemiluminescence assay were also developed,and their performance was compared with that of this novel technology platform.The assay was validated in compliance with the current guidelines.Measurements with the Simoa assay were precise and accurate,presenting a valid assay range from 6.55 to 4000 pg/mL.The intra-and inter-run accuracy and precision were within-19.3%to 15.3%and 5.5%to 17.0%,respectively.S3101 was stable in human serum for 280 days at-20℃and-70℃,for 2 h prior to pre-treatment and 24 h post pre-treatment at room temperature(22℃-28℃),respectively,and after five and two freeze-thaw cycles at-70℃and-20oC,respectively.The Simoa assay also demonstrated sufficient dilution linearity,assay sensitivity,and parallelism for quantifying S3101 in human serum.The Simoa assay is a sensitive and adequate method for evaluating the pharmacokinetic parameters of S3101 in human serum. | Bian-zhen WANG Meng-jia WANG Min-rui WANG Lun OU Li-hou DONG Kelly DONG Hai-feng SONG | 2021 | Current Medical Science2021，41，3： | 1 |
| 4 | Immunostimulatory and anti-neoplasm effects of anovel palindrome CpG oligodeoxynucleotide in mice显示文摘目的: 包含 unmethylated CpG 主题的 DNA 能刺激天生、适应的免疫。这研究的目的是调查新奇 CpG oligodeoxynucleotide 的 immunostimulatory 和反瘤效果, ODN10,在忍受肿瘤的老鼠。 | Hai-yan DU Li-hou DONG Bi-jun ZHAO Jie FU Qing-qing WANG Fang CHEN Lun OU Na LI Xiao SUN Zhong-ming TANG Hai-feng SONG | 2012 | Acta Pharmacologica Sinica2012，33，8： | 0 |
| 5 | Study on Diseased Peanut Leaves by Fourier Transform Infrared Spectroscopy显示文摘[Objective] The paper was to study the application of Fourier transform infrared spectroscopy(FTIR technology) in identification of peanut diseases.[Method] By using FTIR technology,combined with the methods of principal component analysis and hierarchical cluster analysis,the healthy leaves and three kinds of diseased leaves (cercospora black spot,cercospora brown spot and web blotch) were identified in the paper. [Result] IR spectra of both diseased and healthy samples were similar,but tiny differences in wave-numbers and absorption intensities of peaks were observed in the range of 1 750-800 cm-1. Significant differences were found in second derivative spectra in the range of 3 600-2 800 and 1 750-650 cm-1which were selected to perform principle component and hierarchical cluster analysis. Three principal components had the cumulative contribution rate of 94. 9%; the correct rate of principal component analysis for classification was 100% and the correct rate of hierarchical cluster analysis for identification reached 94. 6%. [Conclusion] Fourier transform infrared spectroscopy has a potential to be developed as a powerful means for identification of crop diseases. | Ou Quanhong Zhao Xingxiang Hao Jianming Zhou Xiangping Liu Gang Li Lun Zhang Li Wen Yuanbin | 2013 | Plant Diseases and Pests2013，4，3： | 0 |
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