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| 1 | NOD-like receptors mediated activation of eosinophils interacting with bronchial epithelial cells: a link between innate immunity and allergic asthma显示文摘对细菌的感染的天生的免疫的关键 intracytosolic 模式识别受体是象受体(NLR ) 一样的核苷酸绑定 oligomerization 领域(点头) 。我们阐明了 NOD1 和人的嗜曙红血球的调停 NOD2 的激活,为过敏发炎的主要受动器房间,在在过敏气喘与人的支气管的上皮的 BEAS-2B 房间交往之上。嗜曙红血球组成地表示了 NOD1,2,但是展出了不重要的回答由 NOD1 ligand γ 在刺激之上释放 chemokines; -D-glutamyl-meso-diaminopimelic 酸(即弹跳) 和 NOD2 ligand muramyl dipeptide (MDP ) 。然而,即弹跳和 MDP 能显著地 CD18 的 upregulate 房间表面表示和细胞间的粘附分子(ICAM ) 嗜曙红血球上的 -1 和 BEAS-2B 房间上的 ICAM-1,以及在 coculture 系统导致 chemokines CCL2 和 CXCL8 版本(所有 P < 0.05 ) 。嗜曙红血球和 BEAS-2B 房间在在即弹跳或 MDP 刺激之上的 coculture 系统是为 CXCL8 和 CCL2 版本的主要来源。在嗜曙红血球和 BEAS-2B 房间之间的直接相互作用为 CCL2 版本负责,并且可溶的调停人在 CXCL8 版本被含有。英皇家空军之阶级最低之兵和 NF-κ B 玩为在 coculture 的粘附分子和 chemokines 的表示的规章的角色。有 NOD1,2 ligand 的处理能导致 subepithelial 纤维变性并且显著地提高全部的 IgE,为嗜曙红血球的 chemokine CCL5 和 T 助手类型的浆液集中 2 (Th2 )在 敏化ovalbumin 的过敏气喘的老鼠的 bronchoalveolar lavage 液体的房间和气喘 Th2 cytokine IL-13 (所有 P < 0.05 )。这研究在经由在煽动性的航线与支气管的上皮的房间交往的嗜曙红血球的激活触发过敏气喘提供 NOD1,2 的细菌的调停感染的激活的进一步的证据。 | Churl Kwok Wong Shuiqing Hu Karen Ming-Lam Leungt Jie Dong Lan He Yi Jun Chu Ida Miu-Ting Chu Huai-Na Qiu Kelly Yan-Ping Liu ChristopherWai-Kei Lam | 2013 | Cellular & Molecular Immunology2013，10，4： | 7 |
| 2 | Comparative strategies for stem cell biodistribution in a preclinical study显示文摘Stem cell therapy represents the potential alternative effective strategy for some diseases that lack effective treatment currently.Correspondingly,it is crucial to establish high-sensitive and reliable quanti?cation assay for tracing exogenous cell migration.In the present study,we?rst used both bioluminescence imaging(BLI)indirect labeling(human norepinephrine transporter-luciferase reporter system)and^89zirconium(^89Zr)-hNSCs direct labeling combined with positron emission tomography/computer tomography(PET/CT)system for tracking human neural stem cells(hNSCs)migration into the brain via nasal administration in preclinical study.But the above two methods failed to give the biodistribution pro?le due to their low sensitivity.Considering its superior sensitivity and absolute quantitation capability,we developed and validated the droplet digital PCR(ddPCR)targeting species-speci?c gene in frozen and paraf?n sections,slices,and whole blood with the sensitivity of 100–200 hNSCs.Accurate and high throughput quanti?cation could be performed using ddPCR with the coef?cient of variation(CVs)of lower quality control(LQC)below 30%.In combination with immunohistochemistry and ddPCR,we con?rmed the migration of hNSCs into the brain via nasal administration,which supported the ef?cacy of hNSCs in MPTP-treated mice,an animal model of Parkinson’s disease.In conclusion,the present study is the?rst to report the application of ddPCR in the pharmacokinetics pro?le description of tracking of hNSCs in preclinical studies. | Fang Wang Zhe Wang Fen Wang Kelly Dong Jing Zhang Yun-juan Sun Chun-feng Liu Mei-jie Xing Xue Cheng Su Wei Jia-wei Zheng Xiong-fei Zhao Xiao-ming Wang Jie Fu Hai-feng Song | 2020 | Acta Pharmacologica Sinica2020，41，4： | 2 |
| 3 | Heat budget in the Gulf Stream Region: The importance of heat storage and advection显示文摘 | Dong S F Kelly K | 2004 | Journal of Physical Oceanography2004，34，5： | 1 |
| 4 | Heat budget in the Gulf Stream Region:The importance of heat storage and advection显示文摘 | DONG S F KELLY K A | | 0，，： | 1 |
| 5 | Quantifying the uncertainty in passive microwave snow water equivalent observations显示文摘 | James L. Foster Chaojiao Sun Jeffrey P. Walker Richard Kelly Alfred Chang Jiarui Dong Hugh Powell | 2004 | Remote Sensing of Environment2004，，2： | 1 |
| 6 | Heat budget in the Gulf Stream Region: The importance of heat storage and Advection显示文摘 | Dong Shenfu Kathryn Kelly | 2003 | JPO2003，34，11： | 1 |
| 7 | Heat budget in the gulf stream region:The importance of heat storage and advection显示文摘 | Dong S F Kelly K A | 2003 | J Phys Oceanogr2003，34，5： | 1 |
| 8 | Heat budget in the Gulf Stream region: the importance of heat storage and ad-vection显示文摘 | Dong Shenfu Kelly K A | 2004 | J Phys Oceanogr2004，，： | 1 |
| 9 | Pathological findings following radical prostatectomy in patients who are candidates for active surveillance: impact of varying PSA levels显示文摘活跃监视是在有低风险的前列腺癌症的人的一种可接受的治疗选择。在现在的学习,我们回顾地考察了为活跃监视适合标准,但是选择了激进的前列腺切除术的 509 个人的结果。然后,在 upstaging 的风险上改变前列腺特定的抗原(PSA ) 层次并且在这些病人升级的影响被估计。在三活跃监视 criteria— 下面完成了包括标准的病人的病理学的特征;那些公民加利福尼亚圣弗朗西斯科的大学癌症研究所和 Urology— 的欧洲协会;被检验。被认为的人的比例活跃监视的候选人但是随后 upstaged 或升级被决定。509 个病人, 186 (36.5 %) , 132 (25.9 %) 并且 88 (17.3 %) 人们分别地完成了活跃监视标准。(格利森分数 7-10 ) 升级从 32.8 % ;到 38.6 %,当时 upstaging (≥ pT3 ) 从 10.2 % ;到 12.5 %,取决于三个活跃监视标准。在 24 个月的中部的后续以后,三个病人开发了生物化学的复发。当改变 PSA 层次的影响为每个协议在 PSA 的上下文为趋势分析用测试被检验时, upstaging 的率在有 PSA < 的人是更低的; 4 ng ml −1 。然而,没有在升级上改变 PSA 层次的影响。在结论,通常使用活跃监视协议带升级和 upstaging 的风险。更可靠、精确的标记被需要更好成层是活跃监视的适当候选人的人的风险。 | Dong Il Kang Thomas L. Jang Jeongyun Jeong Eun Young Choi Kelly Johnson Dong Hyeon Lee Wun-Jae Kim Isaac Yi Kim | 2011 | Asian Journal of Andrology2011，13，6： | 1 |
| 10 | Development and Validation of a Simoa Assay for Determination of Recombinant Batroxobin in Human Serum显示文摘Recombinant batroxobin(S3101)is a thrombin-like serine protease that binds to fibrinogen or is taken up by the reticuloendothelial system.A literature survey showed no adequate method that could determine sufficient concentrations to evaluate pharmacokinetic parameters for phase I clinical studies.Therefore,a sensitive method is urgently needed to support the clinical pharmacokinetic evaluation of S3101.In this study,a sensitive bioanalytical method was developed and validated,using a Quanterix single molecular array(Simoa)assay.Moreover,to thoroughly assess the platform,enzyme-linked immunosorbent assay and electrochemiluminescence assay were also developed,and their performance was compared with that of this novel technology platform.The assay was validated in compliance with the current guidelines.Measurements with the Simoa assay were precise and accurate,presenting a valid assay range from 6.55 to 4000 pg/mL.The intra-and inter-run accuracy and precision were within-19.3%to 15.3%and 5.5%to 17.0%,respectively.S3101 was stable in human serum for 280 days at-20℃and-70℃,for 2 h prior to pre-treatment and 24 h post pre-treatment at room temperature(22℃-28℃),respectively,and after five and two freeze-thaw cycles at-70℃and-20oC,respectively.The Simoa assay also demonstrated sufficient dilution linearity,assay sensitivity,and parallelism for quantifying S3101 in human serum.The Simoa assay is a sensitive and adequate method for evaluating the pharmacokinetic parameters of S3101 in human serum. | Bian-zhen WANG Meng-jia WANG Min-rui WANG Lun OU Li-hou DONG Kelly DONG Hai-feng SONG | 2021 | Current Medical Science2021，41，3： | 1 |
| 11 | Expression of connective tissue growth factor (CTGF/CCN2) in breastcancer cells is associated with increased migration and angiogenesis显示文摘 | Wenwen Chien James O’Kelly Daning Lu Amanda Leiter Julia Sohn Dong Yin Beth Karlan Jay Vadgama Karen Lyons H. Koeffler | 2011 | International Journal of Oncology2011，，6： | 1 |
| 12 | Ingestion of Domoic Acid and Its Impact on King Scallop (Pecten maximus, Linnaeus 1758)显示文摘A simple method for spiking formulated feed with domoic acid (DA) was developed in this study. DA feed was prepared by mixing 0.15 mL 100 μg mL-1 DA with 0.1 g formulated feed, and drying the mixture at room temperature for 2 h. The prepared DA feed contained 0.19 pg DA per particle. Of the added DA, 46.72% was retained in the feed. Relatively high DA retention (about 50%) was recorded after DA feed was soaked in water for 2 h. Exposure to DA feed for 7 d did not cause the increase of tissue DA level of adult king scallop (Pecten maximus) significantly in 60 d. The increase of their gonad index after DA exposure was not significantly different from the control. No significant change in DA level was found in spermary, ovary or fertilized eggs after DA exposure. These results indicated that DA excretion may be more efficient than DA accumulation under the current experimental conditions, and the mechanism of domoic acid incorporation in P. maximus may involve intracellular biotransformation. | LIU Hui Maeve S. Kelly Dirk A. Campbell DONG Shuanglin ZHU Jianxin WANG Sufeng | 2007 | Journal of Ocean University of China2007，6，2： | 0 |
| 13 | Decline in subarachnoid haemorrhage volumes associated with the first wave of the COVID- 19 pandemic显示文摘Background During the COVID-19 pandemic,decreased volumes of stroke admissions and mechanical thrombectomy were reported.The study’s objective was to examine whether subarachnoid haemorrhage(SAH)hospitalisations and ruptured aneurysm coiling interventions demonstrated similar declines.Methods We conducted a cross-sectional,retrospective,observational study across 6 continents,37 countries and 140 comprehensive stroke centres.Patients with the diagnosis of SAH,aneurysmal SAH,ruptured aneurysm coiling interventions and COVID-19 were identified by prospective aneurysm databases or by International Classification of Diseases,10th Revision,codes.The 3-month cumulative volume,monthly volumes for SAH hospitalisations and ruptured aneurysm coiling procedures were compared for the period before(1 year and immediately before)and during the pandemic,defined as 1 March-31 May 2020.The prior 1-year control period(1 March-31 May 2019)was obtained to account for seasonal variation.Findings There was a significant decline in SAH hospitalisations,with 2044 admissions in the 3 months immediately before and 1585 admissions during the pandemic,representing a relative decline of 22.5%(95%CI−24.3%to−20.7%,p<0.0001).Embolisation of ruptured aneurysms declined with 1170-1035 procedures,respectively,representing an 11.5%(95%CI−13.5%to−9.8%,p=0.002)relative drop.Subgroup analysis was noted for aneurysmal SAH hospitalisation decline from 834 to 626 hospitalisations,a 24.9%relative decline(95%CI−28.0%to−22.1%,p<0.0001).A relative increase in ruptured aneurysm coiling was noted in low coiling volume hospitals of 41.1%(95%CI 32.3%to 50.6%,p=0.008)despite a decrease in SAH admissions in this tertile.Interpretation There was a relative decrease in the volume of SAH hospitalisations,aneurysmal SAH hospitalisations and ruptured aneurysm embolisations during the COVID-19 pandemic.These findings in SAH are consistent with a decrease in other emergencies,such as stroke and myocardial infarction. | Thanh N Nguyen Diogo C Haussen Muhammad M Qureshi Hiroshi Yamagami Toshiyuki Fujinaka Ossama Y Mansour Mohamad Abdalkader Michael Frankel Zhongming Qiu Allan Taylor Pedro Lylyk Omer F Eker Laura Mechtouff Michel Piotin Fabricio Oliveira Lima Francisco Mont'Alverne Wazim Izzath Nobuyuki Sakai Mahmoud Mohammaden Alhamza R Al-Bayati Leonardo Renieri Salvatore Mangiafico David Ozretic Vanessa Chalumeau Saima Ahmad Umair Rashid Syed Irteza Hussain Seby John Emma Griffin John Thornton Jose Antonio Fiorot Rodrigo Rivera Nadia Hammami Anna M Cervantes-Arslanian Hormuzdiyar H Dasenbrock Huynh Le Vu Viet Quy Nguyen Steven Hetts Romain Bourcier Romain Guile Melanie Walker Malveeka Sharma Don Frei Pascal Jabbour Nabeel Herial Fawaz Al-Mufti Atilla Ozcan Ozdemir Ozlem Aykac Dheeraj Gandhi Chandril Chugh Charles Matouk Pascale Lavoie Randall Edgell Andre Beer-Furlan Michael Chen Monika Killer-Oberpfalzer Vitor Mendes Pereira Patrick Nicholson Vikram Huded Nobuyuki Ohara Daisuke Watanabe Dong Hun Shin Pedro SC Magalhaes Raghid Kikano Santiago Ortega-Gutierrez Mudassir Farooqui Amal Abou-Hamden Tatsuo Amano Ryoo Yamamoto Adrienne Weeks Elena A Cora Rotem Sivan-Hoffmann Roberto Crosa Markus Möhlenbruch Simon Nagel Hosam Al-Jehani Sunil A Sheth Victor S Lopez Rivera James E Siegler Achmad Fidaus Sani Ajit S Puri Anna Luisa Kuhn Gianmarco Bernava Paolo Machi Daniel G Abud Octavio M Pontes-Neto Ajay K Wakhloo Barbara Voetsch Eytan Raz Shadi Yaghi Brijesh P Mehta Naoto Kimura Mamoru Murakami Jin Soo Lee Ji Man Hong Robert Fahed Gregory Walker Eiji Hagashi Steve M Cordina Hong Gee Roh Ken Wong Juan F Arenillas Mario Martinez-Galdamez Jordi Blasco Alejandro Rodriguez Vasquez Luisa Fonseca M Luis Silva Teddy Y Wu Simon John Alex Brehm Marios Psychogios William J Mack Matthew Tenser Tatemi Todaka Miki Fujimura Roberta Novakovic Jun Deguchi Yuri Sugiura Hiroshi Tokimura Rakesh Khatri Michael Kelly Lissa Peeling Yuichi Murayama Hugh Stephen Winters Johnny Wong Mohamed Teleb Jeremy Payne Hiroki Fukuda Kosuke Miyake Junsuke Shimbo Yusuke Sugimura Masaaki Uno Yohei Takenobu Yuji Matsumaru Satoshi Yamada Ryuhei Kono Takuya Kanamaru Masafumi Morimoto Junichi Iida Vasu Saini Dileep Yavagal Saif Bushnaq Wenguo Huang Italo Linfante Jawad Kirmani David S Liebeskind Viktor Szeder Ruchir Shah Thomas G Devlin Lee Birnbaum Jun Luo Anchalee Churojana Hesham E Masoud Carlos Ynigo Lopez Brendan Steinfort Alice Ma Ameer E Hassan Amal Al Hashmi Mollie McDermott Maxim Mokin Alex Chebl Odysseas Kargiotis Georgios Tsivgoulis Jane G Morris Clifford J Eskey Jesse Thon Leticia Rebello Dorothea Altschul Oriana Cornett Varsha Singh Jeyaraj Pandian Anirudh Kulkarni Pablo M Lavados Veronica V Olavarria Kenichi Todo Yuki Yamamoto Gisele Sampaio Silva Serdar Geyik Jasmine Johann Sumeet Multani Artem Kaliaev Kazutaka Sonoda Hiroyuki Hashimoto Adel Alhazzani David Y Chung Stephan A Mayer Johanna T Fifi Michael D Hill Hao Zhang Zhengzhou Yuan Xianjin Shang Alicia C Castonguay Rishi Gupta Tudor G Jovin Jean Raymond Osama O Zaidat Raul G Nogueira SVIN COVID-19 Registry,the Middle East North Africa Stroke and Interventional Neurotherapies Organization(MENA-SINO),Japanese Society of Vascular and Interventional Neurology Society(JVIN) | 2021 | Stroke & Vascular Neurology2021，6，4： | 0 |
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