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4篇 您的检索式:作者名="Luo Yeyuan"
    题名 作者 年代 出处 被引量
1Single- component wormlike micellar system formed by a carboxy- lbetaine surfaetant with C22 saturated tail 显示文摘Zhang Yongmin Luo Yeyuan Wang Yuejiao 2013Colloids and Surfaces A : Physicochem Eng Aspects2013,,:1
2Adsorption of CTAB on zeolite a detected by surfaetant ion - selective electrode显示文摘LUO YEYUAN DU ZHIPING LU YONGHAI 2009Tenside Surfactants Detergents2009,46,:1
3Ultrasound-assisted extraction of polysaccharides from litchi seed by response surface methodology and their structural characteristics 显示文摘Chen Yeyuan Luo Haiyan Gao Aiping 2011Innovative Food Science and Emerging Technologies2011,,30:1
4Molecular Cloning and Sequence Analysis of C4H Gene of Mangifera indica L.显示文摘Cinnamate-4-hydroxylase( C4H) is a key enzyme in phenylpropanoid pathway in plants. Its activity and abundance directly affect the biosynthesis of flavonoids and aromatic compounds. In this study,degenerate primers were designed according to previously reported C4 H gene sequences to clone C4H cDNA sequence with 3'and 5'RACE-PCR from mango( Mangifera indica L). The full-length cD NA of M. indica C4H is 1 680 bp long. Its open reading frame( ORF)is 1 518 bp,encoding a protein of 505 amino acids with a predicted molecular weight of 58. 08 kDa. The isoelectric point of the predicted protein is 9. 52. Functional prediction showed that this gene is mainly located in mitochondria. In addition,the tertiary structure of the protein was built using SWISS-MODEL,and the results showed that the protein has three possible conformations. Phylogenetic analysis based on C4H protein sequences revealed that M. indica has a close genetic relationship with olive( Canarium album) and cocoa( Theobroma cacao). By analyzing the expression level of C4H gene in three colored mango cultivars,we found that that the expression level of C4 H gene in Guifei( with red peel) was the highest,and that in Guiqi( with green peel) was the lowest. The results provide a theoretical basis for studying the molecular mechanism of anthocyanin biosynthesis and C4H's impact on the color of mango fruit.Kuanliang LIU Zhichang ZHAO Aiping GAO Yeyuan CHEN Jianfeng HUANG Zhiguo DANG Ruixiong LUO 2017Agricultural Biotechnology2017,6,5:0
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