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| 1 | A novel organic-inorganic hybrid monolith for trypsin immobilization显示文摘In proteomics, attention has focused on various immobilized enzyme reactors (IMERs) for the realization of high throughput digestion. In this report, a novel organic-inorganic hybrid monolith based IMER was prepared in a 100 μm i.d. capillary with 3-glycidoxypropyltrimethoxysilane (GLYMO) as the monomer and tetraethoxysilane (TEOS) as the crosslinker. Trypsin immobilization was achieved via the reaction between vicinal diol groups, which were obtained from hydrolysis of epoxy groups, and the amino groups of trypsin. Bovine serum albumin was digested thoroughly by this IMER in 47 s. After micro-reverse phase liquid chromatography-tandem mass spectrometry (μRPLC-MS/MS) analysis and database searching, beyond 35% sequence coverage was obtained, and the result was comparable to that of 12 h in solution digestion. The present IMER has potential for high throughput digestion. | WU ShuaiBin MA JunFeng YANG KaiGuang LIU JinXiang LIANG Zhen ZHANG LiHua ZHANG YuKui | 2011 | Science China(Life Sciences)2011，54，1： | 5 |
| 2 | High performance liquid chromatographic separation of eight drugs collected in Chinese Pharmacopoeia 2010 on amylose ramification chiral stationary phase显示文摘The enantiomers separation of ei ght pharmaceutical racemates collected in Chinese Pharmacopoeia 2010(Ch.P2010),including nitrendipine,felodipine,omeprazole,praziquantel,sulpiride,clenbuterol hydrochloride,verapamil hydrochloride and chlorphenamine maleate,was performed on chiral stationary phase of amylose ramification by high performance liquid chromatography(HPLC)on Chiralpak AD-H column and Chiralpak AS-H column with the mobile phase consisted of isopropanol and n-hexane.The detection wavelength and the flow rate were set at 254 nm and 0.7 mL/min,respectively.The effects of proportion of organic additives,alcohol displacer and temperature on the separation were investigated.The results indicated that eight chiral drugs were separated on chiral stationary phase of amylase ramification in normal phase chroma tographic system.The chromatographic retention and resolution of enantiomers were adjusted by factors,including the changes of the concentration of alcohol displacer in mobile phase,organic alkaline modifier and column temperature.It was shown that the resolution was improved with reducing concentration of alcohol displacer.When the concentration of organic alkaline modifier was 0.2%,the resolution and the peak shape were fairly good.Most racemates mentioned above had the best resolution at column temperature of 25℃.The best temperature should be kept unchanged in the process of separation so as to obtain stable separation results. | Yan Wang Ying Zhou Chao Ma Beibei Yang Ru Feng Yiying Zhang Jie Fu Wenjing Chen Yupeng Sun Jingyi Ma Qiming Zhang Yulin Deng Yukui Zhang Wenyi He | 2012 | Acta Pharmaceutica Sinica B2012，2，5： | 3 |
| 3 | Fatty acid composition of Camellia oleifera oil 显示文摘 | MA Jinlin YE Hang RUI Yukui | 2010 | Journal of Consumer Protection and Food Safety2010，6，： | 1 |
| 4 | Fatty acid composition of Camellia oleifera oil显示文摘 | Jinlin Ma Hang Ye Yukui Rui | 2011 | J Verbr Lebensm2011，，6： | 1 |
| 5 | Fatty acid compositionof Camellia ole if era oil 显示文摘 | MA Jinlin YE Hang RUI Yukui ef al | 2011 | Journal of Consumer Protection andFood Safety2011，，6： | 1 |
| 6 | Fatty acid composition of Camellia oleifera oil 显示文摘 | Ma Jinlin Ye Hang Rui Yukui | 2011 | J Verbrauch Lebensm2011，6，1： | 1 |
| 7 | Fatty acid composition of Camellia oleifera oil显示文摘 | MA Jinlin YE Hang RUI Yukui | 2011 | Journal Fur Verbraucherschutz Und Lebensmittelsicherheit-Journal of Consumer Protection and Food Safety2011，6，1： | 1 |
| 8 | Fatty acid composition of Camellia oleifera oil 显示文摘 | MA Jinlin HANG Ye RUI Yukui | 2011 | Journal Fur Verbrauccherschutz Und Lebensmittelsicherheit2011，6，1： | 1 |
| 9 | Fatty Camellia oleifera oil 显示文摘 | Jinlin Ma Hang composition of nal of Consumer (6) : 9-12 Ye Yukui Rui | 2011 | Protection and Food Safety2011，2011，： | 1 |
| 10 | A rapid protein sample preparation method based on organic-aqueous microwave irradiation technique显示文摘Fast and efficient sample preparation methods are a prerequisite for protein identification in bottom-up proteomics. Here, an innovative microwave irradiation sample preparation method was developed based on an optimized organic-aqueous solvent system for protein identification. Specifically, protein solutions containing high-concentration acetonitrile were subjected to 5 min microwave irradiation. After cooling down, trypsin was added and the digestion was performed with 30 s microwave irradiation, and the resulting peptides were analyzed by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry(MALDI-TOF MS). A shortened processing time of only 5.5 min is needed with this method(more than 12 h is necessary in the traditional overnight protein sample preparation). Moreover, due to the absence of urea and other chaotropic reagents, the digests can be readily identified by MALDI-TOF MS. When an assessment of this method was performed by digesting a model protein BSA, 69% ± 3% sequence coverage corresponding to 47 ± 3 peptides was obtained, which shows better protein identification than that from the standard overnight protein sample preparation method(51% ± 2% sequence coverage and 23 ± 1 peptides). Another model protein α-casein was used for the analysis of protein phosphorylation with the newly developed method that yielded 4 phosphopeptides with 8 phosphorylation sites, whereas 3 phosphopeptides with 2 phosphorylation sites were obtained from the traditional overnight approach. Moreover, the organic-aqueous microwave irradiation method provides effective digestion for proteins down to fmol. | Shuaibin Wu Hongfei Zhang Kaiguang Yang Junfeng Ma Zhen Liang Lihua Zhang Yukui Zhang | 2015 | Science China Chemistry2015，58，3： | 0 |
| 11 | Preliminary experience in adult-to-adult living donor liver transplantation in a single center in China显示文摘The aim of this paper is to report the authors’experience in performing adult-to-adult living donor liver transplantation(LDLT)by using a modified technique in using grafts of the right lobe of the liver.From January 2002 to September 2006,56 adult patients underwent LDLT using right lobe grafts at the West China Hospital,Sichuan University Medical School,China.All patients underwent a modified operation designed to improve the reconstruction of the right hepatic vein(RHV)and the tributariers of the middle hepatic vein(MHV)by interposing a vessel graft,and by anastomosing the hepatic arteries and bile ducts.There were no severe complications or deaths in all donors.Fifty-two(92.8%)recipients survived the operations.Among the 56 recipients,complications were seen in 15 recipients(26.8%),including hepatic vein stricture(one case),small-for-size syndrome(one case),hepatic artery thrombosis(two cases),intestinal bleeding(one case),bile leakage(two cases),left subphrenic abscess(one case),renal failure(two cases)and pulmonary infection(five cases).Within three months after transplantation,four recipients(7.14%)died due to smallfor-size syndrome(one case),renal failure(one case)and multiple organ failure(two cases).All patients underwent direct anastomoses of the RHV and the inferior vena cava(IVC),and in 23 cases,reconstruction of the right inferior hepatic vein was also done.In 24 patients,the reconstruction of the tributaries of the MHV was also done by interposing a vessel graft to provide sufficient venous outflow.Trifurcation of the portal vein was seen in nine cases.Thus,veno-plasty or separate anastomoses were performed.The graft and recipient body weight ratios(GRWR)were between 0.72%and 1.43%,and in three cases it was<0.8%.The graft weight to recipient standard liver volume ratios(GV/SLV)were between 31.86%and 71.68%,among which four cases had<40%.No“small-for-size syndrome”occurred.With modification of the surgical technique,especially in the reconstruction of the hepatic vein to provide sufficient venous outflow,living donor liver grafts in adults using the right lobe of the liver can become a relatively safe procedure and prevent the“small-for-size syndrome”. | YAN Lunan LI Bo ZENG Yong WEN Tianfu ZHAO Jichun WANG Wentao YANG Jiayin XU Mingqing MA Yukui CHEN Zheyu LIU Jiangwen WU Hong | 2007 | Frontiers of Medicine2007，1，2： | 0 |
| 12 | A Pt/CeO_(2)Hybrid Nanozyme with Stable Peroxidase Activity for the Detection of Acetylcholine显示文摘Acetylcholine(ACh)is a common neurotransmitterl)that is secreted by cholinergic neurons[2]and is found mainly in the peripheral and central nervous systems.[3]ACh transmits nerve signals from presynaptic to postsynaptic cells via synapses. | CUI Xulian MA Baofu PAN Hui XIA Yu LIU Li ZHAO Baofeng LIANG Zhen ZHANG Lihua ZHANG Yukui | 2024 | Chemical Research in Chinese Universities2024，40，2： | 0 |
| 13 | Se-sensitized NIR hot band absorption photosensitizer for anti-Stokes excitation deep photodynamic therapy显示文摘Conventional anti-Stokes materials-involved deep photodynamic therapy(dPDT)requires much high-intensity irradiance due to low photosensitization efficiency.Herein,we proposed a'booster effector'approach to construct highly efficient hot band absorption phototherapeutics for low/biosafety power anti-Stokes light-triggered d PDT.Se,as'booster effector',was introduced into hot band absorption luminophores(HBAs),which not only significantly facilitated intersystem crossing,but also simultaneously enhanced hot band excitation efficiency atν808,as a result successfully enabling excellent photogenerated singlet oxygen capability of HBAs under ultra-low power anti-Stokes excitation(10 mW cm^(-2)in vitro).As far as we know,such low laser power-initiated photosensitization activity has never been reported in the existing anti-Stokes material systems.Importantly,FUC-Se ME can self-assemble into uniform nanospheres in water,greatly boosting cellular uptake(>25-fold larger than FUC-Se),and achieve superior cancer-killing effect(808 nm,10 mW cm^(-2),5 min,the half-maximal inhibitory concentration IC50=1.36μM).After further PEGylation with folate-attached polymer,the resultant FUC-Se ME@FA can effectively enrich at the tumor(signal-to-background ratio,10).Under safety irradiation(330 mW cm^(-2)),FUC-Se ME@FA effectively inhibits deep-seated tumor progression(the tumor growth inhibition rate,84%).This work provides a successful paradigm,possibly being more clinically beneficial than conventional anti-Stokes materials. | Dandan Ma Hui Bian Saran Long Panwang Zhou Ruisong Tian Yingnan Wu Haoying Ge Mingle Li Jianjun Du Jiangli Fan Yukui Zhang Xiaojun Peng | 2022 | Science China Chemistry2022，65，3： | 0 |
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