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| 1 | Simple and customizable method for fabrication of high-aspect ratio microneedle molds using low-cost 3D printing显示文摘We present a simple and customizable microneedle mold fabrication technique using a low-cost desktop SLA 3D printer.As opposed to conventional microneedle fabrication methods,this technique neither requires complex and expensive manufacturing facilities nor expertise in microfabrication.While most low-cost 3D-printed microneedles to date display low aspect ratios and poor tip sharpness,we show that by introducing a two-step“Print&Fill”mold fabrication method,it is possible to obtain high-aspect ratio sharp needles that are capable of penetrating tissue.Studying first the effect of varying design input parameters and print settings,it is shown that printed needles are always shorter than specified.With decreasing input height,needles also begin displaying an increasingly greater than specified needle base diameter.Both factors contribute to low aspect ratio needles when attempting to print submillimeter height needles.By setting input height tall enough,it is possible to print needles with high-aspect ratios and tip radii of 20–40μm.This tip sharpness is smaller than the specified printer resolution.Consequently,high-aspect ratio sharp needle arrays are printed in basins which are backfilled and cured in a second step,leaving sub-millimeter microneedles exposed resulting microneedle arrays which can be used as male masters.Silicone female master molds are then formed from the fabricated microneedle arrays.Using the molds,both carboxymethyl cellulose loaded with rhodamine B as well as polylactic acid microneedle arrays are produced and their quality examined.A skin insertion study is performed to demonstrate the functional capabilities of arrays made from the fabricated molds.This method can be easily adopted by the microneedle research community for in-house master mold fabrication and parametric optimization of microneedle arrays. | Kevin J.Krieger Nicky Bertollo Manita Dangol John T.Sheridan Madeleine M.Lowery Eoin D.O’Cearbhaill | 2019 | Microsystems & Nanoengineering2019，5，1： | 4 |
| 2 | Porcine Bocavirus: A 10-Year History since Its Discovery显示文摘Porcine bocavirus(PBoV)is a single-stranded DNA virus,belongs to the genus Bocaparvovirus of family Parvoviridae.It was discovered along with porcine circovirus 2(PCV 2)and torque tenovirus(TTV)in the lymph nodes of pigs suffering from postweaning multisystemic wasting syndrome(PMWS)in Sweden in 2009.PBoV has been reported throughout the world,mostly in weaning piglets,and has a broad range of tissue tropism.Since PBoV is prevalent in healthy as well as clinically infected pigs and is mostly associated with coinfection with other viruses,the pathogenic nature of PBoV is still unclear.Currently,there are no cell lines available for the study of PBoV,and animal model experiments have not been described.This review summarizes the current state of knowledge about PBoV,including the epidemiology,evolution analysis,detection methods,pathogenesis and public health concerns. | Manita Aryal Guangliang Liu | 2021 | Virologica Sinica2021，36，6： | 3 |
| 3 | Influence of elevated temperatures on the mechanical properties of blended cement concretes prepared with limestone and siliceous aggregates显示文摘 | SAWA A MANITA P SIDERIS K K | | 0，，02： | 1 |
| 4 | Residual mechanical characteristics and spalling resistance of fiber reinforcedself-compacting concretes exposed to elevated temperatures显示文摘 | Sideris K K Manita P | 2013 | Construction and Building Materials2013，41，： | 1 |
| 5 | Influence of elevated temperatures on the mechanical properties of blended cement concretes prepared with limestone and siliceous aggregates显示文摘 | A. Savva P. Manita K.K. Sideris | 2004 | Cement and Concrete Composites2004，，2： | 1 |
| 6 | Development and validation of bioanalytical methods for Imidafenacin (KRP-197/ 0N0-8025) and its metabolites in human plasma by liquid chro- matography-tandem mass spectrometry显示文摘 | MASUDA Y KANAYAMA N MANITA S | 2007 | J Chromatogr B Analyt Technol Biomed Life Sci2007，853，12： | 1 |
| 7 | Influenceofelevatedtempera-tures on the mechanical properties of blended cement concretes pre-pared with limestone and siliceous aggregates显示文摘 | SAVVA A MANITA P SIDERISKK | 2005 | Cement&ConcreteComposites2005，27，2： | 1 |
| 8 | Development and validation of bioanalytical methods for imidafenacin(KRP-197/ONO-8025)and its metabolites in human plasma by liquid chromatography-tandem mass spectrometry 显示文摘 | MASUDA Y KANAYAMA N MANITA S | 2007 | J Chromatogr B Analyt Technol Biomed Life Sci2007，853，12： | 1 |
| 9 | Influence of elevated temperatures on the mechanical properties of blended cement concretes prepared with limestone andsiliceous aggregates 显示文摘 | SAVVA A MANITA P SIDERIS K K | 2005 | Cement and Concrete Composites2005，27，2： | 1 |
| 10 | Mechanical charac- teristic of high performance fiber reinforced concretes at elevated temperatures显示文摘 | Sideris K Manita P Papageorgiou A | 2006 | ACI Special Publication2006，，： | 1 |
| 11 | Menopausal age and XRCC1 gene polymorphisms: Role in breast cancer risk显示文摘 | Susana N. Silva Rita Moita Ana Paula Azevedo Rita Gouveia Isabel Manita Julieta Esperan?a Pina José Rueff Jorge Gaspar | 2007 | Cancer Detection and Prevention2007，，4： | 1 |
| 12 | Perform- ance of thermally damaged fibre reinforced concretes显示文摘 | SIDERIS K K MANITA P CHANIOTAKIS E | 2009 | Con- struction and Building Materials2009，，23： | 1 |
| 13 | Performance of ther- many damaged fibre reinforced concretes显示文摘 | Sideris K K Manita P Chaniotakis E | 2009 | Constmction and Building Materials2009，，3： | 1 |
| 14 | Deposition of carbon-films by electrolysis of a water-ethylene glycol solution显示文摘 | Suzuki T Manita Y Yamazaki T | 1995 | J Mater Sci1995，30，： | 1 |
| 15 | Estimation of ulti- mate modulus of elasticity and Poisson ratio of normal concrete显示文摘 | Sideris K K Manita P Sideris K | 2004 | Cement and Concrete Composites2004，26，6： | 1 |
| 16 | Influence of elevated temperatures on the mechanical properties of blended cement concretes prepared with limestone and siliceous aggregates显示文摘 | A. Savva P. Manita K.K. Sideris | 2004 | Cement and Concrete Composites2004，，2： | 1 |
| 17 | Prevalence of carotid artery disease in an ischemic stroke population : role of Doppler uhrasonography 显示文摘 | FRAGATA I GALO S MANITA M | 2006 | Acta Med Port2006，19，6： | 1 |
| 18 | 猪博卡病毒VP1蛋白的多克隆抗体制备及初步应用显示文摘旨在制备猪博卡病毒(porcine bocavirus, PBoV)VP1蛋白的多克隆抗体。利用PCR技术对PBoV VP1蛋白的基因进行扩增,将所获基因克隆入原核表达载体,将重组质粒转化大肠杆菌感受态细胞中诱导表达。纯化后的目的蛋白与ISA206佐剂混合后通过背部皮下免疫日本大耳白兔,3次加强免疫后,收集兔血清,利用间接ELISA、Western blot与间接免疫荧光(IFA)的方法验证并评价多克隆抗体的效价与反应性。结果显示,成功获得PBoV VP1蛋白目的基因,利用原核表达系统大量表达了VP1蛋白,并制备多克隆抗体;ELISA滴定结果显示,该抗体在稀释至12 800倍时反应性依旧良好;Western blot检测结果显示,制备的多克隆抗体可与VP1蛋白条带发生特异性结合;将PBoV感染的猪肠道组织制备冰冻切片,IFA检测结果表明,该抗体与PBoV具有良好的反应性。上述结果表明,本研究成功制备了针对PBoV VP1蛋白的兔多克隆抗体,为深入研究PBoV的VP1蛋白生物学功能奠定了一定物质基础。 | 张韬 李茂林 邹抒洁 Manita Aryal 刘光亮 付钰广 | 2021 | 畜牧与兽医2021，53，12： | 1 |
| 19 | Electrophysiological characteristics and radiofrequency ablation of accessory pathways with slow conductive properties显示文摘 | Kaneko Y Kurabayashi M | 2004 | Circ J2004，68，12： | 1 |
| 20 | Performance of thermally damaged fibre reinforced concretes 显示文摘 | Sideris K K Manita P Chaniotakis E | 2009 | Constxuction and Building Materials2009，23，3： | 1 |
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