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| 1 | High-throughput screening for amyloid-β binding natural small-molecules based on the combinational use of biolayer interferometry and UHPLC−DAD-Q/TOF-MS/MS显示文摘Discovery of drugs rapidly and effectively is an important aspect for Alzheimer's disease(AD).In this study,a novel high-throughput screening(HTS)method aims at screening the small-molecules with amyloid-β(Aβ)binding affinity from natural medicines,based on the combinational use of biolayer interferometry(BLI)and ultra-high-performance liquid chromatography coupled with diode-array detector and quadrupole/time-of-flight tandem mass spectrometry(UHPLC−DAD-Q/TOF-MS/MS)has been firstly developed.Briefly,the components in natural medicines disassociated from biotinylated Aβwere collected to analyze their potential Aβbinding affinity by UHPLC−DAD-Q/TOF-MS/MS.Here,baicalein was confirmed to exhibit the highest binding affinity with Aβin Scutellaria baicalensis.Moreover,polyporenic acid C(PPAC),dehydrotumulosic acid(DTA),and tumulosic acid(TA)in Kai-Xin-San(KXS)were also identified as potent Aβinhibitors.Further bioactivity validations indicated that these compounds could inhibit Aβfibrillation,improve the viability in Aβ-induced PC-12 cells,and decrease the Aβcontent and improve the behavioral ability in Caenorhabditis elegans.The molecular docking results confirmed that PPAC,DTA,and TA possessed good binding properties with Aβ.Collectively,the present study has provided a novel and effective HTS method for the identification of natural inhibitors on Aβfibrillation,which may accelerate the process on anti-AD drugs discovery and development. | Minsong Guo Fengdan Zhu Wenqiao Qiu Gan Qiao Betty Yuen-Kwan Law Lu Yu Jianming Wu Yong Tang Chonglin Yu Dalian Qin Xiaogang Zhou Anguo Wu | 2022 | Acta Pharmaceutica Sinica B2022，12，4： | 3 |
| 2 | Cryo-EM structures of human m^(6)A writer complexes显示文摘N6-methyladenosine(m^(6)A)is the most abundant ribonucleotide modification among eukaryotic messenger RNAs.The m^(6)A“writer”consists of the catalytic subunit m^(6)A-METTL complex(MAC)and the regulatory subunit m^(6)A-METTL-associated complex(MACOM),the latter being essential for enzymatic activity.Here,we report the cryo-electron microscopy(cryo-EM)structures of MACOM at a 3.0-Åresolution,uncovering that WTAP and VIRMA form the core structure of MACOM and that ZC3H13 stretches the conformation by binding VIRMA.Furthermore,the 4.4-Åresolution cryo-EM map of the MACOM–MAC complex,combined with crosslinking mass spectrometry and GST pull-down analysis,elucidates a plausible model of the m^(6)A writer complex,in which MACOM binds to MAC mainly through WTAP and METTL3 interactions.In combination with in vitro RNA substrate binding and m^(6)A methyltransferase activity assays,our results illustrate the molecular basis of how MACOM assembles and interacts with MAC to form an active m^(6)A writer complex. | Shichen Su Shanshan Li Ting Deng Minsong Gao Yue Yin Baixing Wu Chao Peng Jianzhao Liu Jinbiao Ma Kaiming Zhang | 2022 | Cell Research2022，32，11： | 3 |
| 3 | PET/CT在前列腺癌放疗规划中的应用显示文摘放疗及根治性前列腺切除术是局限性前列腺癌的最终治疗选择。根治性前列腺切除术后前列腺特异抗原水平升高提示前列腺癌复发,此类患者仍可通过挽救性放疗治愈。为了使治愈机会最大化,放疗靶区应包括疾病的所有范围。因此,无论是根治性还是挽救性放疗,准确估计疾病位置对放疗规划至关重要。目前一线显像方法对前列腺癌的早期分期和生化复发的检测灵敏度均有限。将PET纳入前列腺癌患者的常规评估可以提高分期的准确性和放疗计划的规范性。目前,18F-脱氧葡萄糖(FDG)PET/CT已被常规用于多种癌症的放疗规划,但其对前列腺癌的灵敏度较低。其他评估前列腺癌的PET探针包括18F-NaF、11C-乙酸盐、11C或18F标记的胆碱、抗1-氨基酸-3-18F-氟环丁烷-1羧酸(18F-fluciclovine)和68Ga或18F标记的配体,均可与前列腺特异膜抗原(PSMA)结合。PSMA似乎是最灵敏和特异的配体,但其尚未获得美国食品与药品监督管理局(FDA)的新药申请批准。回顾性和前瞻性研究显示,PET/CT对前列腺放疗计划可能有潜在的重要价值。将患者随机分配到常规放疗计划与PET/CT辅助放疗计划的前瞻性试验或许可以得出很有意义的临床结果。目前正在进行前瞻性临床试验,评估利用18F-fluciclovine PET/CT对具有临床终点的挽救性放疗进行规划;建议进行前瞻性试验,评估PSMA PET/CT对前列腺放疗计划的临床影响。 | 李冠男(译) 李林(审校) Jeremie Calais Minsong Cao Nicholas G.Nickols | 2020 | 中华核医学与分子影像杂志2020，40，9： | 2 |
| 4 | Long-acting antibacterial activity of quaternary phosphonium salts functionalized few-layered graphite 显示文摘 | Xie Agui Cai Xiang Lin Minsong | 2011 | Materials Science and Engineering: B2011，176，15： | 1 |
| 5 | Causes of the soft budget con- straint:Evidence on three explanations 显示文摘 | Li D Liang Minsong | 1998 | Journal of Comparative Economics1998，26，1： | 1 |
| 6 | Synergistic antibacterial brilliant blue/reduced graphene oxidequaternary phosphonium salt composite with excellent water solubility and specific targeting capa- bility 显示文摘 | Cai Xiang Tan Shaozao Lin Minsong | 2011 | Langmuir2011，27，12： | 1 |
| 7 | Causes of the Soft Budget Constraint: Evidence on Three Explanations显示文摘 | David D. Li Minsong Liang | 1998 | Journal of Comparative Economics1998，，1： | 1 |
| 8 | The use of polyethyleneimine-modified reduced graphene oxide as a substrate for silver nanoparticles to produce a material with lower cytotoxicity and long-term antibacterial activity显示文摘 | Xiang Cai Minsong Lin Shaozao Tan Wenjie Mai Yuanming Zhang Zhiwen Liang Zhidan Lin Xiuju Zhang | 2012 | Carbon2012，，10： | 1 |
| 9 | Synergistic Antibacterial Brilliant Blue/ Reduced Graphene Oxide/ Quaternary Phosphonium Salt Composite with Excellent Water Solubility and Specific Targeting Capabilit显示文摘 | Cai Xiang Tan Shaozao Lin Minsong | 2011 | Langmuir2011，27，： | 1 |
| 10 | The use of polyethyleneimine-modified reduced graphene oxide as a substrate for silver nanoparticles to produce a material with lower cytotoxicity and long-term antibacterial activity显示文摘 | Xiang Cai Minsong Lin Shaozao Tan Wenjie Mai Yuanming Zhang Zhiwen Liang Zhidan Lin Xiuju Zhang | 2012 | Carbon2012，，10： | 1 |
| 11 | The use of polyeth- yleneimine-modified reduced grapheme oxide as a substrate for silver nanoparticles to produce a material with lower cy- totoxicity and long-term antibacterial activity显示文摘 | Cai Xiang Lin Minsong Tan Shaozao | 2012 | Carbon2012，50，： | 1 |
| 12 | Sea sonal pattern of infestation by pink bollworm Pectino phora gossypiella (Saunders) in field plots of Bt trans genic cotton in the Yangtze River Valley of China显示文摘 | Wan Pen Wu Kongmin Huang Minsong et at | 2004 | Crop Protection2004，23，5： | 1 |
| 13 | Causes of the Soft Budget Constraint:Evidence on Three Explanations 显示文摘 | David D Li and Minsong Liang | 1998 | Journal of Comparative Economics1998，26，1： | 1 |
| 14 | Integrin β3 and its ligand regulate the expression of uPA through p38 MAPK in breast cancer显示文摘 | RUIWANG ZHAO‐QINGLI XUHAN BAI‐LINLI XIAO‐YIMI LI‐MEISUN MINSONG YAN‐CHUNHAN YANZHAO EN‐HUAWANG | 2010 | APMIS2010，，12： | 1 |
| 15 | Investigation of digital sun sensor technology with an N-shaped slit mask 显示文摘 | WEI Minsong XING Fei LI Bin | 2011 | Sensors2011，11，10： | 1 |
| 16 | companion:Oa智能音箱显示文摘Oa是一个帮助你睡眠的伴音扬声器。作为同伴演讲者,允许同伴与Oa交互,而不是单向通信。Oa在白天听外面的声音,在晚上使用它们来产生有助于睡眠的白噪声。此外,它还为同伴提供了适合心率和温暖度的振动,以便在Oa和同伴之间形成更深的联系,并且同伴可以与Oa一起睡得更香,同伴将有情绪治疗和更舒适的床。 | Minsong Cho Subin Kim WooJin Shin HyoRyung Choi | 2022 | 五金科技2022，50，5： | 0 |
| 17 | METAGENOMICS COMBINED WITH HIGHTHROUGHPUT SEQUENCING REVEALS THE METHANOGENIC POTENTIAL OF FRESH CORN STRAW UNDER THERMOPHILIC AND HIGH OLR显示文摘Dry corn straw(DCS)is usually used in anaerobic digestion(AD),but fresh corn straw(FCS)has been given less consideration.In this study,the thermophilic AD of single-substrate(FCS and DCS)and co-digestion(straw with cattle manure)were investigated.The results show that when FCS was used as the single-substrate for AD,the methane production was 144 mL·g^(−1)·VS^(−1),which was 7.5%and 19.6%higher than that of single DCS and FCS with cattle manure,respectively.In addition,the structure of FCS was loose and coarse,which was easier to be degraded than DCS.At the hydrolysis and acidification stages,Clostridium_sensu_stricto_1,Clostridium_sensu_stricto_7 and Sporosarcina promoted the decomposition of organic matter,leading to volatile fatty acids(VFAs)accumulation.Methanosarcina(54.4%)activated multifunctional methanogenic pathways to avoid the VFAs inhibition,which was important at the CH_(4) production stage.The main pathway was hydrogenotrophic methanogenesis,with genes encoding formylmethanofuran dehydrogenase(K00200-K00203)and tetrahydromethanopterin Smethyltransferase(K00577-K00584).Methanosarcina also activated acetotrophic and methylotrophic methanogenesis pathways,with genes encoding acetyl phosphate(K13788)and methyl-coenzyme M reductase(K04480,K14080 and K14081),respectively.In the co-digestion,the methanogenic potential of FCS was also confirmed.This provides a scientific basis for regulating AD of crop straw. | Jinzhi HUANG Xiaoting YAN Zhen LIU Mengyi WANG Yangyang HU Zhenyu LI Minsong LIN Yiqing YAO | 2023 | Frontiers of Agricultural Science and Engineering2023，10，3： | 0 |
| 18 | Key progresses of MOE key laboratory of macromolecular synthesis and functionalization in 2022显示文摘In 2022,The MOE Key Laboratory of Macromolecular Synthesis and Functionalization in Zhejiang University had achieved several important results.First,a series of well-defined dinuclear organoboron catalysts were developed to precisely control the enchainment of ether and carbonate segments during the copolymerization of CO_(2)and epoxides.Second,polyester had been synthesized through cationic copolymerization of cyclic anhydride.Third,ring-opening polymerization of carbon dioxide based valerolactone had been achieved,revealing the prospect of 3-ethylidene-6-vinyltetrahydro-2H-pyran-2-one(EVL)in utilizing CO_(2)and synthesizing functional polymers.Fourth,machine learning methods have been applied to biomaterial research,enabling high-throughput screening of functional biomaterial surfaces for implantable devices,and searching for potent antimicrobial peptides in whole combinatorial peptide libraries.Fifth,methods of characterization of biomacromolecule RNA transcription and manipulation of nucleoside modification were developed.Sixth,artificial enzymes-armed Bifidobacterium Longum probiotics were established to tune down gut inflammation.Seventh,three-dimensional(3D)printing technologies were used to engineer tough supramolecular hydrogels.Eighth,hydroplastic foaming graphene frameworks for acoustic and conductive polymer composites were provided for application.Ninth,aggregate photophysics about the nature of through-space interactions(TSIs)and manipulating their strength in small molecules with non-conjugated structure had been elucidated.Tenth,the forming mechanism of a newfound nested texture in poly(L-lactic acid)(PLLA)spherulitic films had been revealed.Finally,the isotropically dyeing mechanism of KDP single crystals grown from hydrogels have been explored.The related works are reviewed in this paper. | Xumeng Deng Kaihao Chen Kai Pang Xiaoting Liu Minsong Gao Jie Ren Guanwen Yang Guangpeng Wu Chengjian Zhang Xufeng Ni Peng Zhang Jian Ji Jianzhao Liu Zhengwei Mao Ziliang Wu Zhen Xu Haoke Zhang Hanying Li | 2024 | Chinese Chemical Letters2024，35，3： | 0 |
| 19 | A step‐growth strategy to grow vertical porous aromatic framework nanosheets on graphene oxide:Hybrid material‐confined Co for ammonia borane methanolysis显示文摘The rational synthesis of a two-dimensional(2D)porous aromatic framework(PAF)with a controllable growth direction remains a challenge to overcome the limitation of traditional stacked 2D materials.Herein,a step-growth strategy is developed to fabricate a vertically oriented nitrogen-rich porous aromatic framework on graphene oxide(V-PAF-GO)using monolayer benzidine-functionalized GO(BZ-GO)as a molecular pillar.Then,the confined Co nanoparticle(NP)catalysts are synthesized by encapsulating ultra-small Co into the slit pores of V-PAF-GO.Due to the high nitrogen content,large specific surface area,and adequate slit pores,the optimized vertical nanocomposites V-PAF-GO provide abundant anchoring sites for metal NPs,leading to ultrafine Co NPs(1.4 nm).The resultant Co/V-PAF-GO catalyst shows an extraordinary catalytic activity for ammonia borane(AB)methanolysis,yielding a turnover frequency value of 47.6 min−1 at 25°C,comparable to the most effective non-noble-metal catalysts ever reported for AB methanolysis.Experimental and density functional theory studies demonstrate that the electron-donating effect of N species of PAF positively corresponds to the low barrier in methanol molecule activation,and the cleavage of the O–H bond in CH3OH has been proven to be the rate-determining step for AB methanolysis.This work presents a versatile step-growth strategy to prepare a vertically oriented PAF on GO to solve the stacking problem of 2D materials,which will be used to fabricate other novel 2D or 2D–2D materials with controllable orientation for various applications. | Xiugang Li Qilu Yao Rongwei Shi Minsong Huang Zhang‐Hui Lu | 2023 | Carbon Energy2023，5，10： | 0 |
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