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| 1 | A Robust CRISPR/Cas9 System for Convenient, High-Efficiency Multiplex Genome Editing in Monocot and Dicot Plants显示文摘CRISPR/Cas9 染色体指向系统被用于许多种类。然而,为植物报导的 mostCRISPR/Cas9 系统能仅仅修改一个或一些指向地点。这里,我们报导一个柔韧的 CRISPR/Cas9 向量系统,利用植物 codon 优化了 Cas9 基因,为方便、高效率的复合染色体在 monocot 和 dicot 植物编辑。我们设计了基于 PCR 的过程很快产生多重 sgRNA 表达式盒子,它能在一个轮由金门结扎或吉布森汇编克隆被装配进二进制 CRISPR/Cas9 向量。与这个系统,我们与 85.4% 变化评估的一般水准在米饭编辑了 46 个目标地点,主要在 biallelic 和同型结合的地位。我们推想在米饭的大约 16% 同型结合的变化通过相应基于再结合的修理跟随的非相应的加入结束的机制被产生。我们也获得了一致 biallelic,在 Arabidopsis T 1 植物的异质接合、同型结合、妄想的变化。在米饭和 Arabidopsis 的指向的变化是可继承的。我们在 T 0 米饭和 T 1 由同时的指向的 Arabidopsis 植物多重(多达八) 一个基因家庭,在一条 biosynthetic 小径的多重基因,或在单个基因的多重地点的成员。这个系统为为基本研究和基因改进在植物学习多重基因和基因家庭的功能提供了一个万用的工具箱。 | Xingliang Ma Qunyu Zhang Qinlong Zhu Wei Liu Yan Chen Rong Qiu Bin Wang Zhongfang Yang Heying Li Yuru Lin Yongyao Xie Rongxin Shen Shuifu Chen Zhi Wang Yuanling Chen Jingxin Guo Letian Chen Xiucai Zhao Zhicheng Dong Yao-Guang Liu | 2015 | Molecular Plant2015，8，8： | 272 |
| 2 | DSDecode: A Web-Based Tool for Decoding of Sequencing Chromatograms for Genotyping of Targeted Mutations显示文摘Dear Editor, The transcription activator-like effector nucleases (TALENs) and clustered regularly interspaced short palindromic repeats (CRISPR)/Cas9 genome editing systems have greatly improved the efficiency for generating targeted mutations in various organisms including plants (Li et al.,2012;Cong et al.,2013;Li et al.,2013;Feng et al.,2014;Ma et al.,2015b;Zhang et al.,2014,2015). | Weizhi Liu Xianrong Xie Xingliang Ma Jun Li Jiehu Chen Yao-Guang Liu | 2015 | Molecular Plant2015，8，9： | 62 |
| 3 | CRISPR-GE: A Convenient Software Toolkit for CRISPR-Based Genome Editing显示文摘 | Xie, Xianrong Ma, Xingliang Zhu, Qinlong Zeng, Dongchang Li, Gousi Liu, Yao-Guang | 2017 | Molecular Plant2017，10，9： | 67 |
| 4 | DRISPR/Cas9 Platforms for Genome Editing in Plants: Developments and Applications显示文摘 | Xingliang Ma Qinlong Zhu Yuanling Ghen Yao-Guang Liu | 2016 | Molecular Plant2016，9，7： | 39 |
| 5 | SMXL6/7/8:Dual-Function Transcriptional Repressors of Strigolactone Signaling显示文摘Strigolactones(SLs)were recently identified as a new class of carotenoid-derived plant hormones that regulates many aspects of plant development and responses to both biotic and abiotic stresses.Active SLs are synthesized from its substrateβ-carotene with four successively acting enzymes,including DWARF27(D27),DWARF17(D17),DWARF10(D10),and MORE AXILLARY GROWTH 1(MAX1),and perceived by a noncanonicalα/β-hydrolase receptor DWARF14(D14).In the present of SLs,D14 is activated by ligand hydrolysis and conformational change. | Rongxin Shen Xingliang Ma Haiyang Wang | 2020 | Molecular Plant2020，13，9： | 1 |
| 6 | Effect of hydrophobicity coating on insulator icing and DC flashover per formance of iced insulators显示文摘 | Jiang Xingliang Ma Jun Zhang Zhijing | 2010 | IEEE Transactions on Dielectrics and Electrical Insulation2010，17，2： | 1 |
| 7 | Effect of hydrophobicity coating on insulator icing and DC flashover performance of iced insulators显示文摘 | Jiang Xingliang Ma Jun Zhang Zhijin | 2010 | IEEE Transactions on Dielectrics And Electrical Insulation2010，17，2： | 1 |
| 8 | Preparation of the radioactive source core of iodine-125 seed显示文摘Iodine-125 seed is widely used in brachytherapy of intraocular tumors,brain tumors,and prostate cancer.The1 25I seed is prepared by depositing 125I on a silver rod.This work was aimed at investigating effects of different parameters on the iodine adsorption,so as to optimize the process for preparing1 25I core of the seed.The parameters investigated include kind and concentration of halogenation reagent,halogenation time,adsorption time,pH and carrier iodine quantity. | HE Jiaheng JIANG Lin LI Xingliang ZHONG Wenbin WANG Jing MA Zongping JIAN Yuan | 2009 | Nuclear Science and Techniques2009，20，4： | 1 |
| 9 | Genome Editing: Small-Size CasΦ Is Shining显示文摘CRISPR-Cas-mediated genome editing technology is revolutionizing fundamental biological and biomedical research,as well as human disease therapy and crop breeding.To date,various genome-editing tools utilizing Cas9,Cas12a,Cas12b,and CasX have been developed.However,these widely used Cas proteins are relatively big nucleases,typically close to or more than 1000 amino acids in length.This poses some challenges for in vivo gene manipulations,such as difficulties in cloning and molecular manipulations,and low efficiency in delivery of the ribonucleoprotein(RNP)complex into the cell nucleus for transgene-free genome editing(Ran et al.,2015). | Xingliang Ma Joanne RAshnest | 2020 | Molecular Plant2020，13，9： | 0 |
| 10 | Huge Abdominal Incisional Herina:A Case Report显示文摘Abdominal incisional hernia,an iatrogenic abdominal hernia,is one of the long-term complications of abdominal surgery.Acute incarcerated hernia is a common surgical emergency.In the article,we reported a rare case of huge abdominal incarcerated hernia.Specifically,an elderly female patient complained of reducible mass after abdominal surgery for 30 years,which suddenly became irreducible four days ago,and was treated at the emergency department.Right abdominal incisional hernia accompanied by incarcerated small intestine was indicated by abdominal computed tomography(CT).To the authors’knowledge,this is the first case report of a patient with huge abdominal incisional hernia. | Luyao Ma Xingliang Fang Huanrong Lan Jindao Wang Ketao Jin | 2020 | Journal of US-China Medical Science2020，17，3： | 0 |
| 11 | A Study of the Artificial Vegetation Restoration Technology in the Wenchuan Earthquake Area显示文摘The situation of plants on the slope can reflect the effect of vegetation restoration during the process of artificial vegetation recovery.Taking the typical damaged slope of Wenchuan earthquake area as the research object,through observing the vegetation situation of deserted slope,the results show that compositae plants and gramineous plants are suitable for being pioneer plants and dominant in community; during the vegetation succession,many compositae and gramineous species invade,but there is no magaphanerophytes invading; as time goes by,the herbaceous species and diversity increase gradually,so the ecosystem becomes more stable and the gradient is important for the vegetation restoration. | Yu WANG Xingliang LIU Anmin MIN Li WANG Wenbao MA Hongli PAN Hongxia LI Li ZHANG | 2014 | Asian Agricultural Research2014，6，9： | 0 |
| 12 | Tracing the Origins of Agricultural Products with Barcoded Microbial Spores显示文摘The globalization of agricultural trade has greatly improved our access to cereals,vegetables,and fruits.However,complicated trade routes and supply chains make it challenging to trace the origin of agricultural products.This is particularly problematic when the source of food contamination cannot be determined with the currently available methods.For example,a recent recall of contaminated romaine lettuce from North American shelves caused significant economic losses and raised public concerns on food safety before a lengthy investigation pinpointed the origin of the outbreak.A recent study by Springer's team from the Harvard Medical School has described the so-called Barcoded Microbial Spores(BMS)method,which enables safe,rapid,and high-resolution tracing of the origins of agricultural products(Qian et al.,2020). | Xingliang Ma Joanne R.Ashnest | 2020 | Molecular Plant2020，13，8： | 0 |
| 13 | Addressing the Chengjiang conundrum: A palaeoecological view on the rarity of hurdiid radiodonts in this most diverse early Cambrian Lagerstatte显示文摘Over one hundred arthropod fossil species have been described from the famous Chengjiang Lagerst?tte(South China, Cambrian Stage 3, ca. 518 Ma) including a diverse assemblage of radiodonts–a group containing Anomalocaris and its relatives. These iconic stem-group euarthropods include some of the largest animals of the time, and some are known from hundreds of specimens. A longstanding conundrum has been the rarity or absence of hurdiids from Cambrian Series 2 Lagerst?tten like Chengjiang. This is because radiodonts are generally common in such deposits and the oldest radiodont ever discovered is a hurdiid. Furthermore, this family displays the widest geographic and temporal ranges of all radiodont families, and the highest diversity. Here we document the first hurdiid frontal appendages from Chengjiang, which display unique features within the family and may provide insights for understanding the character evolution of hurdiid appendages. The palaeoenvironmental distribution of hurdiids suggests that the rarity of hurdiids in Chengjiang may be due to a preference for deeper water environments,and the later success of this family from the Wuliuan onwards may relate to their ability to tolerate cooler water temperatures than other radiodont families. The palaeogeographical, palaeoenvironmental, and stratigraphical patterns observed in hurdiids maybe caused in part by the limited distributions of Konservat-Lagerst?tten in the Cambrian as well. | Yu Wu Stephen Pates Jiaxin Ma Weiliang Lin Yuheng Wu Xingliang Zhang Dongjing Fu | 2022 | Geoscience Frontiers2022，13，6： | 0 |
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