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| 1 | CRISPR-GE: A Convenient Software Toolkit for CRISPR-Based Genome Editing显示文摘 | Xie, Xianrong Ma, Xingliang Zhu, Qinlong Zeng, Dongchang Li, Gousi Liu, Yao-Guang | 2017 | Molecular Plant2017，10，9： | 67 |
| 2 | CRISPR/Cas植物基因组编辑技术研究进展显示文摘基因编辑技术的发展与应用为植物功能基因研究和作物遗传改良提供了重要的技术支撑。近年诞生的CRISPR/Cas 基因编辑系统(主要包括 CRISPR/Cas9 和 CRISPR/Cas12a)与其他的基因编辑技术相比,具有操作简单、效率高等优势,因此在动植物中均得到广泛应用。本文结合 CRISPR/Cas 基因编辑技术体系的发展历史及最新研究进展,着重介绍了该技术在植物领域中的应用范围和发展方向,以及基因编辑植物的靶点分析方法;对目前CRISPR/Cas 基因编辑技术体系存在的问题进行了分析并提出了改进策略。 | 刘耀光 李构思 张雅玲 陈乐天 | 2019 | 华南农业大学学报2019，40，5： | 56 |
| 3 | Construction of a Genome-Wide Mutant Library in Rice Using CRISPR/Cas9显示文摘 | Meng, Xiangbing Yu, Hong Zhang, Yi Zhuang, Fengfeng Song, Xiaoguang Gao, Songsong Gao, Caixia Li, Jiayang | 2017 | Molecular Plant2017，10，9： | 46 |
| 4 | DRISPR/Cas9 Platforms for Genome Editing in Plants: Developments and Applications显示文摘 | Xingliang Ma Qinlong Zhu Yuanling Ghen Yao-Guang Liu | 2016 | Molecular Plant2016，9，7： | 39 |
| 5 | 植物CRISPR/Cas9多基因编辑载体构建和突变分析的操作方法显示文摘CRISPR/Cas9基因组编辑技术是植物基因功能研究与作物改良的有效工具.为此,本实验室开发了高效的CRISPR/Cas9植物多基因编辑载体系统.本载体系统包括6个双元载体和12个含有不同U3/U6启动子的sg RNA中间载体,可满足对单子叶和双子叶植物的遗传转化以及不同抗生素筛选的要求,具有简便、高效,可同时对多基因进行编辑的特点.此外,为了能更高效地应用基因组编辑技术,还开发了一站式在线分析工具包CRISPR-GE.为方便研究人员利用CRISPR/Cas9系统进行植物基因组编辑,本文提供了从靶点选择、CRISPR/Cas9多靶点双元载体构建,以及对靶点突变序列的测序分析等详细的操作方法,以及常见的问题解答. | 曾栋昌 马兴亮 谢先荣 祝钦泷 刘耀光 | 2018 | 中国科学:生命科学2018，48，7： | 33 |
| 6 | Genome Engineering in Rice Using Cas9 Variants that Recognize NG PAM Sequences显示文摘CRISPR/Cas9 genome editing relies on sgRNA-target DNA base pairing and a short downstream PAM sequence to recognize target DNA. The strict protospacer adjacent motif (PAM) requirement hinders applications of the CRISPR/Cas9 system since it restricts the targetable sites in the genomes. xCas9 and SpCas9-NG are two recently engineered SpCas9 variants that can recognize more relaxed NG PAMs, implying a great potential in addressing the issue of PAM constraint. Here we use stable transgenic lines to evaluate the efficacies of xCas9 and SpCas9-NG in performing gene editing and base editing in rice. We found that xCas9 can efficiently induce mutations at target sites with NG and GAT PAM sequences in rice. However, base editors containing xCas9 failed to edit most of the tested target sites. SpCas9-NG exhibited a robust editing activity at sites with various NG PAMs without showing any preference for the third nucleotide after NG. Moreover, we showed that xCas9 and SpCas9-NG have higher specificity than SpCas9 at the CGG PAM site. We further demonstrated that different forms of cytosine or adenine base editors containing SpCas9-NG worked efficiently in rice with broadened PAM compatibility. Taken together, our work has yielded versatile genome-engineering tools that will significantly expand the target scope in rice and other crops. | Kai Hua Xiaoping Tao Peijin Han Rui Wang Jian-Kang Zhu | 2019 | Molecular Plant2019，12，7： | 22 |
| 7 | 植物CRISPR/Cas9基因组编辑系统与突变分析显示文摘基因组定点编辑技术通过可编码核酸酶切割基因组特定位点,进而诱导基因组定点突变。CRISPR/Cas9(Clustered regularly interspaced short palindromic repeats/CRISPR-associated protein 9)基因组编辑系统由Cas9核酸酶以及sg RNA(Single guide RNA)组成,与其他可编码核酸酶系统如锌指核酸酶(Zinc finger nucleases,ZFNs)和类转录激活因子效应物核酸酶(Transcription activator-like effector nucleases,TALENs)相比具有更简便的操作性和更高的基因组定点编辑效率。目前在植物中已有多例应用CRISPR/Cas9系统进行基因组编辑的报道。本文从Cas9基因与sg RNA表达载体的构建策略,获得基因组定点编辑突变体的转化方法、突变的效率和特征、突变的检测方法等方面进行了总结,最后对植物中利用CRISPR/Cas9系统进行基因组编辑存在的问题以及发展前景进行了讨论。 | 马兴亮 刘耀光 | 2016 | 遗传2016，38，2： | 18 |
| 8 | 基于CRISPR/Cas9技术的水稻温敏不育基因tms5突变体的构建显示文摘为创新优良温敏不育系,促进两系杂交稻育种的发展,我们以水稻温敏不育基因TMS5为编辑对象,设计了由水稻U3启动子驱动、长20 bp的guide RNA(g RNA)靶点以靶向编辑TMS5基因的第1个外显子,将靶点与表达载体p CAMBIA1301连接,再用农杆菌介导法获得水稻转基因株系。提取T0代转基因株系的基因组DNA,并对TMS5编辑位点附近的DNA片段进行PCR检测及测序分析。结果表明,T0代植株的突变率为63.89%,其中纯合缺失突变率为34.78%。对T1代纯合缺失突变体的不育起点温度和农艺性状调查分析结果表明,28℃是tms5突变体花粉育性的转换温度。大田试验结果表明,与野生型相比,tms5突变体的结实率和单株重均显著降低。粳稻tms5突变体的获得为培育粳稻不育系奠定了材料基础。 | 黄忠明 周延彪 唐晓丹 赵新辉 周在为 符星学 王凯 史江伟 李艳锋 符辰建 杨远柱 | 2018 | 作物学报2018，44，6： | 18 |
| 9 | 利用CRISPR/Cas9技术高效创制长粒香型水稻显示文摘【目的】CRISPR/Cas9基因编辑技术已成为水稻分子育种的重要手段。为了促进水稻育种的发展,本研究以非香型粳稻品种龙粳11为试验材料,对GS3、GS9和Badh2基因进行编辑,以期获得能稳定遗传的长粒香水稻材料。【方法】利用CRISPR/Cas9技术,以GS3、GS9和Badh2为靶基因,构建敲除载体pYLCRISPR/Cas9-GS3/GS9/Badh2-gRNA,通过农杆菌介导法,在龙粳11的GS3、GS9和Badh2基因中引入了特定的突变。【结果】T2代无转基因的gs3/gs9/badh2纯合突变体与野生型龙粳11相比,粒长增加26.43%~27.01%,单株产量增加10.82%~12.11%,千粒重增加18.34%~41.36%,稻米变香,高效地将圆粒水稻变成长粒香型水稻。【结论】利用CRISPR/Cas9技术获得能够稳定遗传并具有长粒香品质的纯合突变株系,为组合多个品质性状提供了一种方便有效的方法,从育种角度加快了新品系创制过程。 | 徐善斌 郑洪亮 刘利锋 卜庆云 李秀峰 邹德堂 | 2020 | 中国水稻科学2020，34，5： | 18 |
| 10 | 基因组编辑技术在水稻功能基因组和遗传改良中的应用显示文摘基因组编辑技术对植物基因功能研究和作物遗传改良具有巨大的潜在价值。CRISPR/Cas9系统是继锌指核酸酶(ZFNs)和类转录激活效应因子核酸酶(TALENs)系统之后的新一代基因组编辑技术系统,具有操作简单和效率高等优点。概述了CRISPR/Cas9系统的技术特点及其在水稻基因功能研究及遗传改良中的应用,并指出了该系统在植物基因精准编辑中需要突破的关键问题。 | 李希陶 刘耀光 | 2016 | 生命科学2016，28，10： | 15 |
| 11 | Characterization and Evaluation of OsLCT1 and OsN ramp5 Mutants Generated Through CRISPR/Cas9-Mediated Mutagenesis for Breeding Low Cd Rice显示文摘To explore how rice(Oryza sativa L.) can be safely produced in Cd-polluted soil, OsLCT1 and OsNramp5 mutant lines were generated by CRISPR/Cas9-mediated mutagenesis. One of OsLCT1 mutant(lct1×1) and two of OsNramp5 mutants(nramp5×7 and nramp5×9) were evaluated for grain Cd accumulation and agronomic performances. In paddy field soil containing approximately 0.9 mg/kg Cd, lct1×1 grains contained approximately 40%(0.17 mg/kg) of the Cd concentration of the wild type parental line, less than the China National Food Safety Standard(0.20 mg/kg). Both OsNramp5 mutants showed low grain Cd accumulation(< 0.06 mg/kg) in the paddy(approximately 0.9 mg/kg Cd) or in pots in soil spiked with 2 mg/kg Cd. However, only nramp5×7 showed normal growth and yield, whereas the growth of nramp5×9 was severely impaired. The study showed that lct1×1 could be used to produce rice grains safe for human consumption in lightly contaminated paddy soils and nramp5×7 used in soils contaminated by much higher levels of Cd. | LIU Songmei JIANG Jie LIU Yang MENG Jun XU Shouling TAN Yuanyuan LI Youfa SHU Qingyao HUANG Jianzhong | 2019 | Rice science2019，26，2： | 15 |
| 12 | 利用CRISPR/Cas9技术编辑Badh2基因改良粳稻香味显示文摘CRISPR/Cas9基因编辑技术已经成为水稻育种新型手段,香稻育种一直是水稻育种行业的研究热点,水稻香味主要由8号染色体上的甜菜碱脱氢酶基因Badh2控制。为了改良原有品种的香味性状,促进香稻育种的发展,以非香型粳稻品种东农425为试验材料,构建了2个CRISPR/Cas9敲除载体对Badh2基因进行定点编辑,第1个载体(pYLCRISPR/Cas9-B1-gRNA)的2个靶点分别位于第2和第3外显子上,第2个载体(pYLCRISPR/Cas9-B2-gRNA)的2个靶点均在第2外显子上。采用农杆菌介导法对东农425进行遗传转化,成功获得了T0纯合植株,并对其衍生出的T1植株进行T-DNA元件检测,共获得8个突变类型不同且不含有转基因成分的纯合突变株系。同时采用咀嚼法和氢氧化钾浸泡法对这8个纯合突变株系的水稻籽粒进行香味检测,结果表明,8个Badh2株系均具有不同程度的香味,总体上载体pYLCRISPR/Cas9-B1-gRNA编辑的3个株系的香味更为浓郁。对这8个纯合突变株系的主要农艺性状进行考察,发现突变株系的穗数、穗粒数、结实率及千粒质量与野生型相比均没有明显差异。综上,本研究对东农425的Badh2基因成功地进行了编辑,并获得了香味显著提高且无转基因成分的纯合突变体材料,为加快香型粳稻品种的培育提供了技术支持。 | 孙慧宇 宋佳 王敬国 刘化龙 孙健 莫天宇 徐善斌 郑洪亮 邹德堂 | 2019 | 华北农学报2019，34，4： | 13 |
| 13 | Increasing yield potential through manipulating of an ARE1 ortholog related to nitrogen use efficiency in wheat by CRISPR/Cas9显示文摘Wheat(Triticum aestivum L.)is a staple food crop consumed by more than 30%of world population.Nitrogen(N)fertilizer has been applied broadly in agriculture practice to improve wheat yield to meet the growing demands for food production.However,undue N fertilizer application and the low N use efficiency(NUE)of modern wheat varieties are aggravating environmental pollution and ecological deterioration.Under nitrogen-limiting conditions,the rice(Oryza sativa)abnormal cytokinin response1 repressor1(are1)mutant exhibits increased NUE,delayed senescence and consequently,increased grain yield.However,the function of ARE1 ortholog in wheat remains unknown.Here,we isolated and characterized three TaARE1 homoeologs from the elite Chinese winter wheat cultivar ZhengMai 7698.We then used CRISPR/Cas9-mediated targeted mutagenesis to generate a series of transgene-free mutant lines either with partial or triple-null taare1 alleles.All transgene-free mutant lines showed enhanced tolerance to N starvation,and showed delayed senescence and increased grain yield in field conditions.In particular,the AABBdd and aabbDD mutant lines exhibited delayed senescence and significantly increased grain yield without growth defects compared to the wild-type control.Together,our results underscore the potential to manipulate ARE1 orthologs through gene editing for breeding of high-yield wheat as well as other cereal crops with improved NUE. | Jiahui Zhang Huating Zhang Shaoya Li Jingying Li Lei Yan Lanqin Xia | 2021 | Journal of Integrative Plant Biology2021，63，9： | 13 |
| 14 | 基于CRISPR/Cas9系统的水稻光敏色素互作因子OsPIL15基因编辑显示文摘【目的】光作为一种环境信号,可影响植物的基因表达、酶活性和形态建成。光敏色素互作因子在光信号传导过程中起着重要作用。本研究旨在构建水稻光敏色素互作因子OsPIL15的CRISPR/Cas9表达载体,创制OsPIL15突变体,挖掘水稻功能基因,丰富和完善水稻光信号调控分子机制。【方法】依据CRISPR/Cas9技术原理,设计OsPIL15突变靶点。将所设计靶序列在水稻基因组中进行比对,排除非特异性靶位点,同时使该靶序列含有常用酶切位点,方便后期突变体鉴定。化学合成靶位点寡核苷酸序列并与载体pBUN411连接构建CRISPR/Cas9表达载体,利用农杆菌介导法导入粳稻品种日本晴,以除草剂抗性标记筛选获得阳性转基因植株。利用酶切法判断T0代转基因植株是否发生突变,结合测序结果分析突变单株的突变基因型。将靶点序列在水稻全基因组中进行比对分析,选择5个与靶序列同源性较高且错配在4 bp以内的位点作为潜在脱靶位点进行脱靶效应评估,分析所设计靶序列特异性。【结果】所构建表达载体成功实现了对OsPIL15的定向编辑,酶切显示在选取的25株T0代转基因植株中获得15株突变体,其中包括5株纯合突变体、6株双等位突变体和4株杂合突变体,共10种不同突变基因型和11个突变株系。突变类型以单碱基插入或缺失为主,同时也得到2种56和66 bp较大片段缺失株系。对部分纯合突变、双等位突变和杂合突变体的T1代植株进行分析,结果表明,T0代产生的突变基因型绝大部分能稳定遗传给下一代。T0代纯合突变体后代为纯合突变单株,仅在株系14纯合突变体后代中检测到1株未突变单株;T0代双等位突变体后代可得到2种纯合突变型和1种双等位突变型;T0代杂合突变体后代则可得到纯合、杂合及未突变3种类型。对T0代未突变植株的后继世代酶切分析显示,62株T1代转基因植株均未发生突变,表明CRISPR/Cas9在T1代转基因阳性植株中未重新发挥基因编辑作用。对20株突变体的5个潜在脱靶位点进行分析,5个潜在脱靶位点均未检测出脱靶效应,表明所设计靶序列具有较高特异性。对选取的3组不同基因型ospil15 T1代突变体表型进行初步观察,结果表明,突变体生育期和分蘖数未出现明显变化,株高极显著下降,籽粒粒长极显著增加,最大增幅达5.69%。【结论】CRISPR/Cas9系统能对OsPIL15进行定向编辑,获得的10种不同突变基因型的ospil15突变体与野生型相比株高极显著降低、籽粒粒长极显著增大。 | 季新 李飞 晏云 孙红正 张静 李俊周 彭廷 杜彦修 赵全志 | 2017 | 中国农业科学2017，50，15： | 11 |
| 15 | Suppression or knockout of SaF/SaM overcomes the Sa-mediated hybrid male sterility in rice显示文摘Hybrids between the indica and japonica subspecies of rice(Oryza sativa) are usually sterile, which hinders utilization of heterosis in the inter-subspecific hybrid breeding. The complex locus Sa comprises two adjacently located genes, SaF and SaM, which interact to cause abortion of pollen grains carrying the japonica allele in japonica-indica hybrids. Here we showed that silencing of SaF or SaM by RNA interference restored male fertility in indica-japonica hybrids with heterozygous Sa. We further used clustered regularly interspaced short palindromic repeats(CRISPR)/Cas9-based genome editing to knockout the SaF and SaM alleles, respectively, of an indica rice line to create hybrid-compatible lines. The resultant artificial neutral alleles did not affect pollen viability and other agricultural traits, but did break down the reproductive barrier in the hybrids. We found that some rice lines have natural neutral allele Sa-n, which was compatible with the typical japonica or indica Sa alleles in hybrids. Our results demonstrate that SaF and SaM are required for hybrid male sterility, but are not essential for pollen development. This study provides effective approaches for the generation of hybrid-compatible lines by knocking out the Sa locus or using the natural Sa-n allele to overcome hybrid male sterility in rice breeding. | Yongyao Xie Baixiao Niu Yunming Long Gousi Li Jintao Tang Yaling Zhang Ding Ren Yao-Guang Liu Letian Chen | 2017 | Journal of Integrative Plant Biology2017，59，9： | 11 |
| 16 | CRISPR/Cas基因组编辑技术研究进展及其在植物中的应用显示文摘基因组编辑技术利用人工核酸酶以精准和可预测的方式来快速改造和修饰基因组,加速了基础研究和植物育种的进程。近年来兴起的II型CRISPR/Cas9基因组编辑技术成功应用于多种动植物中。CRISPR/Cas9系统在可定制的小型非编码RNA的指导下定向切割基因组DNA双链,并通过非同源末端连接(NHEJ)和同源重组(HDR)机制进行基因修饰,从而实现基因组靶标基因的定点改造。本综述介绍了CRISPR/Cas系统的结构及作用原理,讨论了CRISPR/Cas9系统的靶点效率、脱靶效应以及Cas9突变体的筛选,并对CRISPR/Cas9系统的表观遗传调控进行了分析。最后我们详细阐述了CRISPR/Cas9技术在植物中的最新应用进展,并对CRISPR/Cas9系统的发展和应用前景进行了展望,希望为开展植物基因组编辑方面的研究工作提供参考。 | 丁莉萍 张杰伟 马艳 陈亚娟 王宏芝 魏建华 | 2019 | 植物生理学报2019，55，4： | 11 |
| 17 | Targeted mutagenesis of amino acid transporter genes for rice quality improvement using the CRISPR/Cas9 system显示文摘High grain protein content(GPC) reduces rice eating and cooking quality(ECQ). We generated OsAAP6 and OsAAP10 knockout mutants in three high-yielding japonica varieties and one japonica line using the CRISPR/Cas9 system. Mutation efficiency varied with genetic background in the T_0 generation, and GPC in the T_1 generation decreased significantly,owing mainly to a reduction in glutelin content. Amylose content was down-regulated significantly in some Osaap6 and all Osaap10 mutants. The increased taste value of these mutants was supported by Rapid Visco Analysis(RVA) profiles, which showed higher peak viscosity and breakdown viscosity and lower setback viscosity than the wild type. There were no significant deficiencies in agronomic traits of the mutants. Targeted mutagenesis of OsAAP6 and OsAAP10, especially OsAAP10, using the CRISPR/Cas9 system can rapidly reduce GPC and improve ECQ of rice, providing a new strategy for the breeding cultivars with desired ECQ. | Shiyu Wang Yihao Yang Min Guo Chongyuan Zhong Changjie Yan Shengyuan Sun | 2020 | The Crop Journal2020，8，3： | 11 |
| 18 | Editing of Rice Isoamylase Gene ISA1 Provides Insights into Its Function in Starch Formation显示文摘Isoamylase 1(ISA1) is an isoamylase-type debranching enzyme which plays a predominant role in amylopectin synthesis. In this study, the clustered regularly interspaced short palindromic repeats(CRISPR)/CRISPR-associated endonuclease 9(CRISPR/Cas9) system was used to edit ISA1 gene in rice via Agrobacterium-mediated transformation. We identified 36 genetic edited lines from 55 T_0 transgenic events, and classified the mutation forms into 7 types. Of those, two homozygous mutants, cr-isa1-1(type 1, with an adenine insertion) and cr-isa1-2(type 3, with a cytosine deletion) were selected for further analysis. Seed sizes of both cr-isa1-1 and cr-isa1-2 were affected, and the two mutants also displayed a shrunken endosperm with significantly lower grain weight. Electron microscopy analysis showed that abnormal starch granules and amyloplasts were found in cr-isa1-1 and cr-isa1-2 endosperm cells. The contents of total starch, amylose and amylopectin in the endosperm of the cr-isa1 mutants were significantly reduced, whereas sugar content and starch gel consistency were observably increased compared to the wild-type. The gelatinization temperature and starch chain length distributions of the cr-isa1 mutants were also altered. Moreover, transcript levels of most starch synthesis-related genes were significantly lower in cr-isa1 mutants. In conclusion, the results indicated that gene edition of ISA1 affected starch synthesis and endosperm development, and brought potential implications for rice quality breeding. | CHAO Shufen CAI Yicong FENG Baobing JIAO Guiai SHENG Zhonghua LUO Ju TANG Shaoqing WANG Jianlong HU Peisong WEI Xiangjin | 2019 | Rice science2019，26，2： | 10 |
| 19 | 利用CRISPR/Cas9敲除葡萄VviPDS1基因的研究显示文摘选择葡萄八氢番茄红素脱氢酶(Phytoene desaturase)基因VviPDS1为靶标,利用CRISPR/Cas9系统构建基因敲除载体,瞬时转化葡萄叶片原生质体,检测到不同类型的突变。通过农杆菌介导转化‘无核白’葡萄胚性愈伤组织,筛选获得卡那霉素抗性植株71株。经PCR鉴定,其中53株为阳性植株,阳性率为74.64%。测序结果表明,共有20株在靶点发生不同类型的突变,编辑效率为37.74%;其中9株产生了双等位基因突变。对其进行氨基酸序列预测,在第202位氨基酸之后发生了不同程度的变异。利用CRISPR/Cas9系统敲除VviPDS1获得的突变体植株呈现整体矮化,其叶片出现不同程度白化。表明CRISPR/Cas9系统可以通过细胞中的瞬时或稳定表达进行基因编辑,可以实现在葡萄编辑植株中产生纯合敲除。 | 郭晔 万东艳 柴壮壮 王跃进 文颖强 | 2019 | 园艺学报2019，46，4： | 9 |
| 20 | 利用CRISPR/Cas9技术创制抗稻瘟病香型早籼温敏核不育系显示文摘【目的】创制新型抗稻瘟病香型早籼温敏核不育系,为高产优质杂交水稻选育提供资源。【方法】利用CRISPP/Cas9技术在水稻稻瘟病基因Pi21、温敏不育基因TMS5和香味基因Badh2的第1外显子处设计靶位点,构建多基因表达载体pC1300-2×35S::g^(TMS5)-g^(Badh2)-g^(Pi21),转化优质常规籼稻品种中早70,测序鉴定分析获得纯合阳性稳定株系。利用稻瘟病喷雾接种和打孔接种方法对稻瘟病基因Pi21的纯合突变株系进行稻瘟病抗性鉴定,利用GC-MS技术对Badh2纯合突变株系的香味物质2-乙酰-1-吡咯啉(2-AP)含量进行测定。【结果】在T_(0)转基因株系中,Pi21、TMS5和Badh2突变频率分别为87.5%、80.0%和87.5%,突变类型多为双等位突变。从T_(1)代中筛选不含载体骨架的纯合突变株系,获得两种三突变纯合株系。稻瘟病接种结果表明,与野生型相比,T_(2)代Pi21纯合变异株系的抗性显著提高。同时,接种后纯合突变体株系内相关防卫基因的表达量显著上调,ROS积累量也显著增加。tms5纯合变异株系表现出典型的温敏不育特性,TMS5基因的表达水平与野生型相比显著降低,高温下Ub_(L40)4基因的表达水平明显高于野生型。与野生型相比,在Badh2纯合突变体植株中Badh2的表达水平显著下调,并且香味物质2-AP含量极显著增加。【结论】利用CRISPR/Cas9技术成功对Pi21、TMS5和Badh2基因同时进行定向编辑,获得了具有高抗稻瘟病的香型温敏不育系,为高抗、香型不育系材料的选育提供参考,加快高产优质杂交水稻的选育。 | 梁敏敏 张华丽 陈俊宇 戴冬青 杜成兴 王惠梅 马良勇 | 2022 | 中国水稻科学2022，36，3： | 9 |
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