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| 1 | Sodium glucose co-transporter 2 inhibition reduces succinate levels in diabetic mice显示文摘BACKGROUND Type 1 diabetes(T1D) is associated with major chronic microvascular complications which contribute significantly to diabetes associated morbidity.The protein primarily responsible for glucose reabsorption in the kidney is sodium glucose co-transporter 2(SGLT2). Presently, SGLT2 inhibitors are widely used in diabetic patients to improve blood glucose levels and prevent cardiovascular and renal complications. Given the broad therapeutic application of SGLT2 inhibitors, we hypothesised that SGLT2 inhibition may exert its protective effects via alterations of the gut microbiome and tested this in a type 1 diabetic mouse model of diabetic retinopathy.AIM To determine whether the treatment with two independent SGLT2 inhibitors affects gut health in a type 1 diabetic mouse model.METHODS The SGLT2 inhibitors empagliflozin or dapagliflozin(25 mg/kg/d) or vehicle dimethylsulfoxide(DMSO) were administered to C57 BL/6 J, Akita, Kimba and Akimba mice at 10 wk of age for 8 wk via their drinking water. Serum samples were collected and the concentration of succinate and the short chain fatty acid(SCFA) butyric acid was measured using gas chromatography-mass spectrometry. Enzyme-linked immunosorbent assay(ELISA) was performed to determine the concentration of insulin and leptin. Furthermore, the norepinephrine content in kidney tissue was determined using ELISA. Pancreatic tissue was collected and stained with haematoxylin and eosin and analysed using brightfield microscopy.RESULTS Due to the presence of the Akita allele, both Akita and Akimba mice showed a reduction in insulin production compared to C57 BL/6 J and Kimba mice.Furthermore, Akita mice also showed the presence of apoptotic bodies within the pancreatic islets. The acinar cells of Akita and Akimba mice showed swelling which is indicative of acute injury or pancreatitis. After 8 wk of SGLT2 inhibition with dapagliflozin, the intermediate metabolite of gut metabolism known as succinate was significantly reduced in Akimba mice when compared to DMSO treated mice. In addition, empagliflozin resulted in suppression of succinate levels in Akimba mice. The beneficial SCFA known as butyric acid was significantly increased in Akita mice after treatment with dapagliflozin when compared to vehicle treated mice. The norepinephrine content in the kidney was significantly reduced with both dapagliflozin and empagliflozin therapy in Akita mice and was significantly reduced in Akimba mice treated with empagliflozin.In non-diabetic C57 BL/6 J and Kimba mice, serum leptin levels were significantly reduced after dapagliflozin therapy.CONCLUSION The inhibition of SGLT2 reduces the intermediate metabolite succinate, increases SCFA butyric acid levels and reduces norepinephrine content in mouse models of T1 D. Collectively, these improvements may represent an important mechanism underlying the potential benefits of SGLT2 inhibition in T1 D and its complications. | Lakshini Y Herat Natalie C Ward Aaron L Magno Elizabeth P Rakoczy Marcio G Kiuchi Markus P Schlaich Vance B Matthews | 2020 | World Journal of Gastroenterology2020，26，23： | 3 |
| 2 | Bacterial communities and biogeochemical transformations of iron and sulfur in a high saltmarsh soil profile显示文摘 | Lambais Marcio R Otero Xose L Cury Juliano C | 2008 | Soil Biology & Biochemistry2008，40，： | 1 |
| 3 | Cobalt-and manganese-Substituted ferrites as efficient single-site heterogeneous catalysts for aerobic oxidation of monoterpenic alkenes under solvent-free conditions显示文摘 | Luciano Menini Marcio C Pereira Luciana A Parreira | | 0，，： | 1 |
| 4 | The successive projections algorithm for spectral variable selection in classification problems 显示文摘 | Marcio J C P Roberto K H G Mario C U A | 2005 | Chemometrics and Intelligent Laboratory Systems2005，78，： | 1 |
| 5 | MACROPHAGE MIGRATION INHIBITORY FACTOR LEVELS CORRELATE WITH FATAL OUTCOME IN SEPSIS显示文摘 | Fernando A Bozza Rachel N Gomes André M Japiassú Marcio Soares Hugo C Castro-Faria-Neto Patrìcia T Bozza Marcelo T Bozza | 2004 | Shock2004，，4： | 1 |
| 6 | Blue upeonversion emission of Tm~+-Yb3+ in ZrOz nanoerystals:role of Yb3+ ions显示文摘 | PATRA A SAHA S MARCIO A R C | 2005 | Chemical Physics Letters2005，407，4： | 1 |
| 7 | Lithosphere-asthenosphere interaction and the origin of cretaceous tholelltic magmatism in northeastern Brazil- Sr-Nd-Pb isotopic evidence显示文摘 | MARIA H B M H MARCIO M P DIOGENES C O | 2006 | Lithos2006，86，1： | 1 |
| 8 | Flow Injection Analysis-Amperometric Determination of Ascorbic Acid and Uric Acid in Urine Using Arrays of Gold Microelectrode Modified by Electro Deposition of Palladium 显示文摘 | Renato C M Marcio A A Claudimir L L | 2000 | Anal Chim Acta2000，404，： | 1 |
| 9 | Combined zero-valent iron and fenton processes for the treatment of Brazilian TNT industry wastewater显示文摘 | MARCIO B R FLáVIO T S TERESA C B P | 2009 | Journal of Hazardous Materials2009，165，： | 1 |
| 10 | Different expression of defense- related genes in arbuscular mycorrhiza显示文摘 | Marcio R Lambais Mona C M | 1995 | Can J Bot1995，73，： | 1 |
| 11 | Esophagogastric junction outflow obstruction successfully treated with laparoscopic Heller myotomy and Dor fundoplication: First case report in the literature显示文摘BACKGROUND Esophagogastric junction outflow obstruction(EGJOO) is a rare syndrome,characterized by an elevation of the integrated relaxation pressure of the lower esophageal sphincter, not accompanied by alterations in esophageal motility that may lead to the criteria for achalasia. We were unable to find any prior report of the combination of Heller myotomy with anterior partial fundoplication(Dor) as the treatment for EGJOO. We herein report a case of EGJOO treated with laparoscopic Heller myotomy combined with Dor fundoplication.CASE SUMMARY A 26-year-old man presented with a 3-year history of solid dysphagia and a 30-kg weight loss. He was treated with oral nifedipine, isosorbide, and omeprazole,without resolution of symptoms. An upper gastrointestinal series(barium swallow) revealed a 'bird's beak' sign. Esophagogastroduodenoscopy was positive for Los Angeles grade A peptic esophagitis. High-resolution esophageal manometry was compatible with EGJOO. Esophageal pH monitoring showed pathological acid reflux both in orthostatic and decubitus position. An 8-cm laparoscopic Heller myotomy combined with an anterior 220° Dor fundoplication was performed. Solid diet was introduced on postoperative day 2, and the patient was discharged home the same day. At 17-mo follow-up, he reported no symptoms. Barium swallow was compatible with complete radiologic resolution.Both esophageal manometry and upper endoscopy showed normal findings 9 mo after the operation.CONCLUSION Surgical treatment with Heller myotomy and Dor fundoplication is a potential treatment option for EGJOO refractory to medical treatment. | Pedro F Pereira Andre RP Rosa Leonardo A Mesquita Marcelle J Anzolch Rafael N Branchi Augusto L Giongo Francisco C Paix?o Marcio F Chedid Cleber DP Kruel | 2019 | World Journal of Gastrointestinal Surgery2019，11，2： | 1 |
| 12 | Summating potential-action potential waveform amplitude and width in the diagnosis of Meniere′s disease显示文摘 | MARCIO C IKINO Y REZENDE DE ALMEIDA E | 2006 | Laryngoscope2006，116，： | 1 |
| 13 | Noise-to-Harmonics ratio as an acoustic measure of voice disorders in boys显示文摘 | GERALDO P J ONIVALDO C MARCIO A | 2002 | J Voice2002，16，： | 1 |
| 14 | Domain size effects in molecular dynamics simulation of phonon transport in silicon 显示文摘 | ROGERIO F S MARCIO A R C A JANDIR M H | 2003 | Appl Phys Lett2003，89，17： | 1 |
| 15 | A Compact Model of MOSFET Mismatch for Circuit Design显示文摘 | Carlos Galup- Montoro Marcio C Schneider | 2005 | IEEE Solid - State Circuits2005，40，8： | 1 |
| 16 | Single stage self-oscillating HPF electronic ballast 显示文摘 | Marcio B Mdrcio A C Domingos S L S | 2005 | IEEE Transactions on Industry Applications2005，41，3： | 1 |
| 17 | Prop-erties of chemically treated natural amorphous silica fibers aspolyurethane reinforcement显示文摘 | Guilherme M O Barra Marcio C Fredel Hazim A | 2006 | Polymer Composites2006，27，： | 1 |
| 18 | Production of inulinase by solid-state fermentation:effect of process parameters on production and preliminary characterization of enzyme preparations显示文摘 | MARCIO M GUSTAVO C MARCO D L | 2007 | Bioprocess and Biosystems Engineering2007，30，5： | 1 |
| 19 | Physiological features of periodontal regeneration and approaches for periodontal tissue engineering utilizing periodontal ligament cells显示文摘 | Bruno B B Karina G S Marcio Z C | 2007 | J Biosci Bioeng2007，103，1： | 1 |
| 20 | Combustion process in the synthesis of ZnO- Bi2O3显示文摘 | Vania C de Sousa Morelli Marcio R Kiminami Ruth H G | 2000 | Ceramics International2000，26，： | 1 |
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