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| 1 | Intracellular chloride regulates the G_1/S cell cycle progression in gastric cancer cells显示文摘Recent studies show that ion channels/transporters play important roles in fundamental cellular functions. Several reports indicating the important roles of Cl- channels/transporters on cell proliferation suggest that the intracellular chloride concentration ([Cl-]i) regulated by them would be one of critical messengers. We investigated whether the [Cl-]i controls cell proliferation and cell cycle progression in human gastric cancer cells. Our studies indicated that furosemide, a blocker of Na+ /K+ /2Cl- cotransporter (NKCC), diminished cell growth by delaying the G1-S phase progression in gastric cancer cells with high expression and activity of NKCC. Furthermore, we found that the culture in the low Cl- medium (replacement of Cl- by NO3-) decreased the [Cl-]i and inhibited cell growth of gastric cancer cells and that this inhibition of cell growth was due to cell cycle arrest at the G0/G1 phase caused by diminutionof CDK2 and phosphorylated Rb. The culture of cells in the low Cl- medium significantly increased expressions of p21 mRNA and protein. In addition, the low Cl- medium induced phosphorylation of mitogen activated protein kinases (MAPKs). Treatment with an inhibitor of p38 or JNK significantly suppressed p21 upregulation caused by culture in a low Cl- medium and rescued gastric cancer cells from the low Cl- -induced G1 cell cycle arrest. These findings revealed that the [Cl-]i affects the cell proliferation via activation of MAPKs through upregulation of p21 in gastric cancer cells. Our results suggest that the [Cl-]i regulates important cellular functions in gastric cancer cells, leading to the development of novel therapeutic strategies. | Atsushi Shiozaki Eigo Otsuji Yoshinori Marunaka | 2011 | World Journal of Gastrointestinal Oncology2011，3，8： | 6 |
| 2 | 加权SPXYE(WSPXYE)算法及其在近红外光谱模型转移中的应用显示文摘样本选择是模型转移的重要组成部分,其目的是在主光谱和从光谱中选择合适的样本,建立二者的转移模型,使得从光谱的预测样本能通过转移模型校正成类似于主光谱的样本,进而用主光谱的模型直接预测其浓度。目前,常用的样本选择算法有:Kennard-Stone法(KS法),SPXY法和SPXYE法。根据上述算法的特点,提出了一种新的样本选择方法:加权SPXYE法(WSPXYE法),进而将其用于选择合适的转移集样本。WSPXYE同样先计算样本间的距离,其距离有三个部分组成:光谱(X)之间的归一化距离dxs,浓度(y)之间的归一化距离d_(ys),以及校正误差(e)之间的归一化距离d_(es)。其加权代数和d wspxye=αd_(xs)+βd_(ys)+(1-α-β)d_(es)即为WSPXYE距离。计算了WSPXYE距离之后,可以根据其距离选择距离较大的样本作为转移集样本。WSPXYE是Kennard-Stone法(KS法),SPXY法和SPXYE法的推广,而KS法(α=1,β=0)、SPXY法(α=0.5,β=0.5)以及SPXYE法(α=0.333,β=0.333)则是WSPXYE法的特例。直接校正法(DS)、有信息成分提取-典型相关分析法(CCA-ICE)作为模型转移算法验证了WSPXYE方法的效果。结果显示,与KS法、SPX Y法以及SPXYE法相比,WSPXYE法可以通过调节参数,选择合适的样本,获得较低的误差。 | 郑开逸 封韬 张文 黄晓玮 李志华 张迪 石吉勇 Yoshinori Marunaka 邹小波 | 2021 | 光谱学与光谱分析2021，41，3： | 3 |
| 3 | Roles of interstitial fluid pH in diabetes mellitus: Glycolysis and mitochondrial function显示文摘The pH of body fluids is one the most important keyfactors regulating various cell function such as enzyme activity and protein-protein interaction via modification of its binding affinity. Therefore, to keep cell function normal, the pH of body fluids is maintained constant by various systems. Insulin resistance is one of the most important, serious factors making the body condition worse in diabetes mellitus. I have recently found that the pH of body(interstitial) fluids is lower in diabetes mellitus than that in non-diabetic control, and that the lowered pH is one of the causes producing insulin resistance. In this review article, I introduce importance of body(interstitial) fluid pH in regulation of body function, evidence on abnormal regulation of body fluid pH in diabetes mellitus, and relationship between the body fluid pH and insulin resistance. Further, this review proposes perspective therapies on the basis of regulation of body fluid pH including propolis(honeybee product) diet. | Yoshinori Marunaka | 2015 | World Journal of Diabetes2015，6，1： | 2 |
| 4 | Cellular physiological approach for treatment of gastric cancer显示文摘Recent studies show that ion channels/transporters play important roles in fundamental cellular functions that would be involved in the cancer process. We review the evidence for their expression and functioning in human gastric cancer(GC), and evaluate the potential of cellular physiological approach in clinical management. Various types of ion channels, such as voltage-gated K+ channels, intracellular Cl- channels and transient receptor potential channels have been found to express in GC cells and tissues, and to control cell cycles. With regard to water channels, aquaporin 3 and 5 play an important role in the progression of GC. Regulators of intracellular pH, such as anion exchanger, sodiumhydrogen exchanger, vacuolar H+-ATPases and carbonic anhydrases are also involved in tumorigenesis of GC. Their pharmacological manipulation and gene silencing affect cellular behaviours, suggesting their potential as therapeutic targets for GC. Our studies indicate theintracellular Cl- concentration could act as a mediator of cellular signaling and control cell cycle progression in GC cells. Further, we demonstrate the cytocidal effects of hypotonic shock on GC cells, and indicate that the blockade of Cl- channels/transporters enhances these effects by inhibiting regulatory volume decrease. A deeper understanding of molecular mechanisms may lead to the discovery of these cellular physiological approaches as a novel therapeutic strategy for GC. | Atsushi Shiozaki Daisuke Ichikawa Eigo Otsuji Yoshinori Marunaka | 2014 | World Journal of Gastroenterology2014，20，33： | 2 |
| 5 | J Med Chem 显示文摘 | Yasumoto M Yamawaki I Marunaka T | 1978 | 21:7381978，21，： | 1 |
| 6 | High-performance liquid chromatographic determination of proglumide in plasma 显示文摘 | Marunaka T Minami Y Umeno Y | 1982 | J Pharm Sci1982，71，8： | 1 |
| 7 | Acidic degradation of cefodizime (THR-221) and structural elucidation of the products (Ⅰ)显示文摘 | Marunaka T Matsushima E Minami Y | | 0，，02： | 1 |
| 8 | Role of the Na^+/K^+/2Cl^- cotransporter NKCC1 in cell cycle progression in human esophageal squamous cell carcinoma显示文摘AIM:To investigate the role of Na+/K+/2Cl-cotransporter 1(NKCC1)in the regulation of genes involved in cell cycle progression and the clinicopathological significance of its expression in esophageal squamous cell carcinoma(ESCC).METHODS:An immunohistochemical analysis was performed on 68 primary tumor samples obtained from ESCC patients that underwent esophagectomy.NKCC1expression in human ESCC cell lines was analyzed by Western blotting.Knockdown experiments were conducted using NKCC1 small interfering RNA,and the effects on cell cycle progression were analyzed.The gene expression profiles of cells were analyzed by microarray analysis.RESULTS:Immunohistochemical staining showed that NKCC1 was primarily found in the cytoplasm of carcinoma cells and that its expression was related to the histological degree of differentiation of SCC.NKCC1 was highly expressed in KYSE170 cells.Depletion of NKCC1in these cells inhibited cell proliferation via G2/M phase arrest.Microarray analysis identified 2527 genes with altered expression levels in NKCC1depleted KYSE170.Pathway analysis showed that the top-ranked canonical pathway was the G2/M DNA damage checkpoint regulation pathway,which involves MAD2L1,DTL,BLM,CDC20,BRCA1,and E2F5.CONCLUSION:These results suggest that the expression of NKCC1 in ESCC may affect the G2/M checkpoint and may be related to the degree of histological differentiation of SCCs.We have provided a deeper understanding of the role of NKCC1 as a mediator and/or a biomarker in ESCC. | Atsushi Shiozaki Yoshito Nako Daisuke Ichikawa Hirotaka Konishi Shuhei Komatsu Takeshi Kubota Hitoshi Fujiwara Kazuma Okamoto Mitsuo Kishimoto Yoshinori Marunaka Eigo Otsuji | 2014 | World Journal of Gastroenterology2014，20，22： | 1 |
| 9 | Chloride ions control the G 1 /S cell-cycle checkpoint by regulating the expression of p21 through a p53-independent pathway in human gastric cancer cells显示文摘 | Hiroaki Miyazaki Atsushi Shiozaki Naomi Niisato Rumi Ohsawa Hirosumi Itoi Yuji Ueda Eigo Otsuji Hisakazu Yamagishi Yoshinobu Iwasaki Takashi Nakano Takashi Nakahari Yoshinori Marunaka | 2007 | Biochemical and Biophysical Research Communications2007，，2： | 1 |
| 10 | Genistein enhances the NGF-induced neuriteoutgrowth 显示文摘 | Nakajima K Niisato N Marunaka Y | 2011 | Biomed Res2011，32，5： | 1 |
| 11 | Determination of cefodizime in biological materials by high-performance liquid chromatography显示文摘 | MARUNAKA T MATSUSHIMA E MANIWA M | 1987 | J Chromatogr1987，420，2： | 1 |
| 12 | Synthesis and antitum or activities of 1-(tetrahydro-2-furanyl)-5-fluorouracil and 1,3-bis (tetrahydro-2-furanyl)-5-fluorouracil显示文摘 | Yasumoto M Yamawaki I Marunaka T | 1978 | J Med Chem1978，21，： | 1 |
| 13 | A new macromolecular antitumor antiobiotic, C-1027 isolation and physico-chemical properties显示文摘 | OTANI T MINAMI Y MARUNAKA T | 1988 | Antibiotics1988，41，11： | 1 |
| 14 | Quercetinstimulates NGF-induced neurite outgrowth in PC12 cells via activa-tion of Na+/K+/2Cl-cotransporter显示文摘 | Ken-ichi Nakajima Naomi Niisato Yoshinori Marunaka | 2011 | Cell Physiol Biochem2011，28，1： | 1 |
| 15 | Blockade of Chloride Ion Transport Enhances the Cytocidal Effect of Hypotonic Solution in Gastric Cancer Cells显示文摘 | Daisuke Iitaka Atsushi Shiozaki Daisuke Ichikawa Toshiyuki Kosuga Shuhei Komatsu Kazuma Okamoto Hitoshi Fujiwara Hiromichi Ishii Takashi Nakahari Yoshinori Marunaka Eigo Otsuji | 2012 | Journal of Surgical Research2012，，2： | 1 |
| 16 | Enhancement of the cytocidal effects of hypotonic solution using a chloride channel blocker in pancreatic cancer cells显示文摘 | Yoshito Nako Atsushi Shiozaki Daisuke Ichikawa Shuhei Komatsu Hirotaka Konishi Daisuke Iitaka Hiromichi Ishii Hisashi Ikoma Takeshi Kubota Hitoshi Fujiwara Kazuma Okamoto Toshiya Ochiai Takashi Nakahari Yoshinori Marunaka Eigo Otsuji | 2012 | Pancreatology2012，，5： | 1 |
| 17 | Activation of the Na^+-K^+ pump by hyposmolality through tyrosine kinase-dependent Cl-conductance in Xenopus renal epithelial A6 cells显示文摘 | Niisato N Marunaka Y | 1999 | J Physiol1999，518，2： | 1 |
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