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| 1 | Hybrid Rubisco with Complete Replacement of Rice Rubisco Small Subunits by Sorghum Counterparts Confers C4 Plant-like High Catalytic Activity显示文摘Photosynthetic rate at the present atmospheric condition is limited by the CO2-fixing enzyme ribulose-1,5-bisphosphate carboxylase/oxygenase(Rubisco)because of its extremely low catalytic rate(kcat)and poor affinity for C02(Kc)and specificity for CO2(Sc/O).Rubisco in C4 plants generally shows higher k cat than that in C3 plants.Rubisco consists of eight large subunits and eight small subunits(RbcS).Previously,the chimeric incorporation of sorghum C4-type RbcS significantly increased the k cat of Rubisco in a C3 plant,rice.In this study,we knocked out rice RbcS multigene family using the CRISPR-Cas9 technology and completely replaced rice RbcS with sorghum RbcS in rice Rubisco.Obtained hybrid Rubisco showed almost C4 plant-like catalytic properties,i.e.,higher kcai,higher Kc,and lower Sc/O.Transgenic lines expressing the hybrid Rubisco accumulated reduced levels of Rubisco,whereas they showed slightly but significantly higher photosynthetic capacity and similar biomass production under high C 02 condition compared with wild-type rice.High-resolution crystal structural analysis of the wild-type Rubisco and hybrid Rubisco revealed the structural differences around the central pore of Rubisco and the pC-pD hairpin in RbcS.We propose that such differences,particularly in the pC-pD hairpin,may impact the flexibility of Rubisco catalytic site and change its catalytic properties. | Hiroyoshi Matsumura Keita Shiomi Akito Yamamoto Yuri Taketani Noriyuki Kobayashi Takuya Yoshizawa Shun-ichi Tanaka Hiroki Yoshikawa Masaki Endo Hiroshi Fukayama | 2020 | Molecular Plant2020，13，11： | 8 |
| 2 | Long‐term outcomes of endoscopic submucosal dissection for early gastric cancer: A single‐center retrospective study显示文摘 | Takashi Kosaka Masaki Endo Yosuke Toya Yukito Abiko Norihiko Kudara Masaaki Inomata Toshimi Chiba Yasuhiro Takikawa Kazuyuki Suzuki Tamotsu Sugai | 2014 | Digestive Endoscopy2014，，2： | 4 |
| 3 | Involvement of stomach ghrelin and hypothalamic neuropeptides in tumor necrosis factor-alpha-induced hypophagia in mice显示文摘 | Endo M Masaki T Seik M | 2007 | Regul Pept2007，140，： | 1 |
| 4 | Polymerization of isoprene with Ni(acac)-methylaluminoxane catalyst显示文摘 | Masaki K Uchida Y | 1997 | Polym J1997，29，7： | 1 |
| 5 | C/EBP homologous protein is crucial for the acceleration of experimental pancreatitis显示文摘 | Koichi Suyama Masaki Ohmuraya Masahiko Hirota Nobuyuki Ozaki Satoshi Ida Motoyoshi Endo Kimi Araki Tomomi Gotoh Hideo Baba Ken-ichi Yamamura | 2007 | Biochemical and Biophysical Research Communications2007，，1： | 1 |
| 6 | TNF-αinduces hepatic steatosis in mice by enhancing gene expression of sterol regulatory element binding protein-1c (SREBP-1c)显示文摘 | Endo M Masaki T Seike M | 2007 | Exp Biol Med2007，232，5： | 1 |
| 7 | TNF - alpha induces hepatic steatosis in mice by enhancing gene expression of sterol regulatory element binding protein - lc (SREBP - lc) 显示文摘 | Endo M Masaki T Seike M | 2007 | Exp Biol Med (Maywood)2007，232，5： | 1 |
| 8 | TNF-alpha induces hepatic steatosis in mice by enhancing gene expression of sterol regulatory element binding protein-lc (SREBP-lc) 显示文摘 | Endo M Masaki T Seike M | 2007 | Exp Biol Med2007，232，5： | 1 |
| 9 | TNF-αinduces hepatic steatosis in mice by enhancing gene expression of sterol regulatory element binding protein-1c(SREBP-1c)显示文摘 | Endo M Masaki T Seike M | | 0，，： | 1 |
| 10 | TNF-alpha induces hepatic steatosis in mice by enhancing gene expression of sterol regulatory element binding protein-1c(SREBP-1c)显示文摘 | Endo M1 Masaki T Seike M | 2007 | Exp Biol Med(Maywood)2007，232，5： | 1 |
| 11 | Molecular characterization of true and ectopic gene targeting events at the acetolactate synthase gene in Arabldopsis显示文摘 | Masaki Endo Keishi Osakabe Hiroaki Ichikawa | 2006 | Plant and Cell Physiology2006，47，3： | 1 |
| 12 | Casting of high-aluminum-content Mg alloys strip by a horizontal twin-roll caster显示文摘 | HIDETO H SHINICHI N ENDO MASAKI | 2014 | Matallurgical and Materials Transactions2014，45，： | 1 |
| 13 | Guidelines on nutritional management in Japanese patients with liver cirrhosis from the perspective of preventing hepatocellular carcinoma显示文摘 | Kazuyuki Suzuki Ryujin Endo Yutaka Kohgo Takaaki Ohtake Yoshiyuki Ueno Akinobu Kato Kazutomo Suzuki Ryo Shiraki Hisataka Moriwaki Daiki Habu Masaki Saito Shuhei Nishiguchi Kazuhiro Katayama Isao Sakaida | 2012 | Hepatology Research2012，，7： | 1 |
| 14 | 显示文摘 | Masahiro Hiratsuka Yoh Takekuma Naomi Endo Kaori Narahara Samar Hamdy Yukinaga Kishikawa Masaki Matsuura Yasuyuki Agatsuma Tomoko Inoue Michinao Mizugaki | 2002 | European Journal of Clinical Pharmacology2002，，6： | 1 |
| 15 | Initial Japanese Experience with Intragastric Balloon Placement显示文摘 | Masayuki Ohta Seigo Kitano Seiichiro Kai Akio Shiromizu Hidetoshi Eguchi Yuichi Endo Takayuki Masaki Tetsuya Kakuma Hironobu Yoshimatsu | 2009 | Obesity Surgery2009，，6： | 1 |
| 16 | Association between white opaque substance under magnifying colonoscopy and lipid droplets in colorectal epithelial neoplasms显示文摘AIM To examine the association between white opaque substance(WOS) and histologically verified lipiddroplets in colorectal epithelial neoplasms.METHODS We reviewed colonoscopy records at our institution from 2014 to 2016 and identified cases of endoscopically or surgically resected colorectal epithelial neoplasms observed by magnifying narrow-band imaging(M-NBI) colonoscopy. Immunohistochemistry was used to stain tumors with a monoclonal antibody specific to adipophilin as a marker of lipids. The expression and distribution of adipophilin were compared between WOS-positive and WOS-negative lesions and among tumors classified by histologic type and depth of invasion.RESULTS Under M-NBI colonoscopy, 81 lesions were positive for WOS and 48 lesions were negative for WOS. The rate of adipophilin expression was significantly higher in WOS-positive lesions(95.1%) than in WOS-negative lesions(68.7%)(P = 0.0001). The incidence of deep adipophilin expression was higher in WOS-positive lesions(24.7%) than in WOS-negative lesions(4.2%)(P = 0.001). The incidence of deep expression was predominant among cancers with massive submucosal invasion(62.5%) compared to adenoma(7.2%) and high-grade dysplasia or cancers with slight submucosal invasion(12.7%)(P = 0.0001).CONCLUSION The distribution of lipid droplets may be closely associated with the visibility of WOS under M-NBI colonoscopy, and with histologic grade and depth of tumor invasion. | Keisuke Kawasaki Makoto Eizuka Shotaro Nakamura Masaki Endo Shunichi Yanai Risaburo Akasaka Yosuke Toya Yasuko Fujita Noriyuki Uesugi Kazuyuki Ishida Tamotsu Sugai Takayuki Matsumoto | 2017 | World Journal of Gastroenterology2017，23，47： | 1 |
| 17 | The effect of granulocyte and monocyte adsorptive apheresis on serum cytokine levels in patients with ulcerative colitis显示文摘 | Yosuke Toya Toshimi Chiba Tomomi Mizutani Kunihiko Sato Satoshi Kasugai Nozomi Matsuda Shunsuke Orikasa Sho Shibata Yukito Abiko Risaburo Akasaka Naoki Yokoyama Shuhei Oana Shigeru Hirota Masaki Endo Kazuyuki Suzuki | 2013 | Cytokine2013，，： | 1 |
| 18 | Major patterns of higher teleostean phylogenics:a new perspective based on 100 complete mitochondrial DNA sequences显示文摘 | Masaki Miya Hirohiko Takeshima Hiromitsu Endo | 2003 | Molecular Phylogenetics and Evolution2003，，26： | 1 |
| 19 | TNF-alpha induces hepatic steatosis in mice by enhancing gene expression of sterol regulatory element binding protein-1c (SREBP-lc) 显示文摘 | Endo M Masaki T Seike M | 2007 | Exp Biol Med2007，232，5： | 1 |
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