| 2 | Prepartum dietary energy intake alters adipose tissue transcriptome profiles during the periparturient period in Holstein dairy cows显示文摘Background:The aim of the study was to investigate the effect of energy overfeeding during the dry period on adipose tissue transcriptome profiles during the periparturient period in dairy cows.Methods:Fourteen primiparous Holstein cows from a larger cohort receiving a higher-energy diet(1.62 Mcal of net energy for lactation/kg of dry matter;15%crude protein)for ad libitum intake to supply 150%(OVR)or 100%(CTR)of energy requirements from dry off until parturition were used.After calving,all cows received the same lactation diet.Subcutaneous adipose tissue(SAT)biopsies were collected at−14,1,and 14d fromparturition(d)and used for transcriptome profiling using a bovine oligonucleotide microarray.Data mining of differentially expressed genes(DEG)between treatments and due to sampling time was performed using the Dynamic Impact Approach(DIA)and Ingenuity Pathway Analysis(IPA).Results:There was a strong effect of over-feeding energy on DEG with 2434(False discovery rate-corrected P<0.05)between OVR and CTR at−14 d,and only 340 and 538 at 1 and 14 d.The most-impacted and activated pathways in the Kyoto Encyclopedia of Genes and Genomes(KEGG)database that were highlighted by DIA analysis at−14 d in OVR vs.CTR included 9 associated with carbohydrate metabolism,with‘Pyruvate metabolism’,‘Glycolysis/gluconeogenesis’,and‘Pentose phosphate pathway’among the most-activated.Not surprisingly,OVR led to marked activation of lipid metabolism(e.g.‘Fatty acid biosynthesis’and‘Glycerolipid metabolism’).Unexpected metabolic pathways that were activated at−14 d in OVR included several related to metabolism of amino acids(e.g.branched chain)and of cofactors and vitamins(thiamin).Among endocrine and immune system pathways,at−14 d OVR led to marked activation of‘PPAR signalling’and‘Antigen processing and presentation’.Among key pathways affected over time in OVR,a number were related to translation(e.g.mTOR signaling),endocrine/immune signaling(CXCR4 and IGF1),and lipid metabolism(oxidative phosphorylation)with greater activation in OVR vs.CTR specifically at−14 d.Although statistical differences for several pathways in OVR vs.CTR nearly disappeared at 1 and 14 vs.−14 d,despite the well-known catabolic state of adipose depots after calving,the bioinformatics analyses suggested important roles for a number of signaling mechanisms at−14 vs.14 than 1 vs.-14 d.This was particularly evident in cows fed to meet predicted energy requirements during the dry period(CTR).Conclusions:Data underscored a strong activation by overfeeding energy of anabolic processes in the SAT exclusively prepartum.The study confirmed that higher-energy diets prepartum drive a transcriptional cascade of events orchestrated in part by the activation of PPARγthat regulate preadipocyte differentiation and lipid storage in SAT.Novel aspects of SAT biology to energy overfeeding or change in physiologic state also were uncovered,including the role of amino acid metabolism,mTOR signaling,and the immune system. | Andrea Minuti Massimo Bionaz Vincenzo Lopreiato Nicole A.Janovick Sandra L.Rodriguez-Zas James K.Drackley Juan J.Loor | 2020 | Journal of Animal Science and Biotechnology2020，11，2： | 2 |
| 3 | Discrimination of background events in the PolarLight X-ray polarimeter显示文摘PolarLight is a space-borne X-ray polarimeter that measures the X-ray polarization via electron tracking in an ionization chamber.It is a collimated instrument and thus suffers from the background on the whole detector plane.The majority of background events are induced by high energy charged particles and show ionization morphologies distinct from those produced by X-rays of interest.Comparing on-source and off-source observations,we find that the two datasets display different distributions on image properties.The boundaries between the source and background distributions are obtained and can be used for background discrimination.Such a means can remove over 70%of the background events measured with PolarLight.This approaches the theoretical upper limit of the background fraction that is removable and justifies its effectiveness.For observations with the Crab nebula,the background contamination decreases from 25%to 8%after discrimination,indicative of a polarimetric sensitivity of around 0.2 Crab for PolarLight.This work also provides insights into future X-ray polarimetric telescopes. | Jiahuan Zhu Hong Li Hua Feng Jiahui Huang Xiangyun Long Qiong Wu Weichun Jiang Massimo Minuti Saverio Citraro Hikmat Nasimi Dongxin Yang Jiandong Yu Ge Jin Ming Zeng Peng An Luca Baldini Ronaldo Bellazzini Alessandro Brez Luca Latronico Carmelo Sgrò Gloria Spandre Michele Pinchera Fabio Muleri Paolo Soffitta Enrico Costa | 2021 | Research in Astronomy and Astrophysics2021，21，9： | 0 |
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