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| 1 | Establishing a human pancreatic stem cell line and transplanting induced pancreatic islets to reverse experimental diabetes in rats显示文摘The major obstacle in using pancreatic islet transplantation to cure type I and some type II diabetes is the shortage of the donors. One of ways to overcome such obstacle is to isolate and clone pancreatic stem cells as 'seed cells' and induce their differentiation into functional islets as an abundant trans-plantation source. In this study, a monoclonal human pancreatic stem cell (mhPSC) line was obtained from abortive fetal pancreatic tissues. Pancreatic tissues were taken from abortive fetus by sterile procedures, and digested into single cells and cell clusters with 0.1% type IV collagenase. Cultured in modified glucose-low DMEM with 10% fetal bovine serum (FBS), these single cells and cell clusters adhered to culture dishes, and then primary epidermal-like pancreatic stem cells started to clone. After digesting with 0.25% trypsin and 0.04% EDTA, fibroblasts and other cells were gradually eliminated and epithelioid pancreatic stem cells were gradually purified during generations. Using clone-ring selection, the mhPSCs were obtained. After addition of 10 ng/mL epidermal growth factor (EGF) in cell culture medium, the mhPSCs quickly grew and formed a gravelstone-like monolayer. Continuously proliferated, a mhPSC line, which was derived from a male abortive fetus of 4 months old, has been passed through 50 generations. More than 1×109 mhPSCs were cryo-preserved in liquid nitrogen. Karyotype analysis showed that the chromosome set of the mhPSC line was normal diploid. Immunocytochemistry results demonstrated that the mhPSC line was positive for the pdx1, glucagon, nestin and CK19, and negative for the insulin, CD34, CD44 and CD45 protein expression. RT-PCR revealed further that the mhPSCs expressed transcription factors of the pdx1, glucagon, nestin and CK19. Also, in vitro induced with β-mercaptoethanol, the mhPSCs differentiated into nerve cells that expressed the NF protein. Induced with nicotinamide, the mhPSCs differentiated into functional islet-like clusters, as identified by di-thizone staining, which expressed the transcription factor of the insulin and secreted the insulin and C-peptide. Furthermore, the transplantation of mhPSCs-induced pancreatic islets into the subcapsular region of the kidney in streptozotocin-induced diabetic rats could reduce blood glucose levels and prolong the life time. | XIAO Mei AN LiLong YANG XueYi GE Xin QIAO Hai ZHAO Ting MA XiaoFei FAN JingZhuang ZHU MengYang DOU ZhongYing | 2008 | Science China(Life Sciences)2008，51，9： | 9 |
| 2 | miRNA-181 regulates embryo implantation in mice through targeting leukemia inhibitory factor显示文摘Embryo implantation is a crucial step in mammalian reproduction.However,little is known regarding the physiological roles of microRNAs in the regulation of embryo implantation.Here we show that a minimum uterine expression of miR-181 is essential for the onset of embryo implantation.Both transient and prolonged transgenic expression of miR-181 led to impaired implantation,which can be rescued by exogenous administration of leukemia inhibitory factor(LIF).Mechanistically,miR-181 is able to directly target LIF and downregulate LIF expression,thereby inhibiting embryo implantation.We also show that miR-181 expression is regu-lated by the transcriptional factor Emx2,and the Emx2–miR-181 axis plays an importantrole in regulating embryo implantation.Taken together,these results reveal a novel function of miR-181 in embryo implantation through the regulation of LIF,and also indicate a potential link between miR-181 dysregulation and human embryo implantation defects. | Bo Chu Liangwen Zhong Shuang Dou Jun Wang Jianmin Li Mingwei Wang Qinghua Shi Yide Mei Mian Wu | 2015 | Journal of Molecular Cell Biology2015，7，1： | 5 |
| 3 | Strategies for improving the lithium-storage performance of 2D nanomaterials显示文摘2D nanomaterials, including graphene, transition metal oxide(TMO) nanosheets, transition metal dichalcogenide(TMD) nanosheets, etc., have offered an appealing and unprecedented opportunity for the development of high-performance electrode materials for lithium-ion batteries(LIBs). Although significant progress has been made on 2D nanomaterials for LIB applications in the recent years, some major challenges still exist for the direct use of these sheet-like nanomaterials, such as their serious self-agglomerating tendency during electrode fabrication and low conductivity as well as the large volume changes over repeated charging–discharging cycles for most TMOs/TMDs, which have resulted in large irreversible capacity, low initial Coulombic efficiency and fast capacity fading. To address these issues,considerable progress has been made in the exploitation of 2D nanosheets for enhanced lithium storage. In this review, we intend to summarize the recent progress on the strategies for enhancing the lithium-storage performance of 2D nanomaterials, including hybridization with conductive materials, surface/edge functionalization and structural optimization. These strategies for manipulating the structures and properties of 2D nanomaterials are expected to meet the grand challenges for advanced nanomaterials in clean energy applications and thus provide access to exciting materials for achieving high-performance next-generation energy-storage devices. | Jun Mei Yuanwen Zhang Ting Liao Ziqi Sun Shi Xue Dou | 2018 | National Science Review2018，5，3： | 5 |
| 4 | Ultrasonic diagnosis of patients with clonorchiasis and preliminary study of pathogenic mechanism显示文摘Objective:To discuss the liver function damage mechanism of patients with clonorchiasis by analyzing the ultrasound characteristics,liver function,change of the serum inflammatory factors and cell apoptosis factors.Methods:Color Doppler ultrasound technique was adopted to detect the portal vein and blood flow change of patients with clonorchiasis;ELISA was used to determine the level of different serum inflammatory factors.The levels of serum total bilirubin,serum albumin and glutamic-pyruvic transaminase were detected by automatic biochemical analyzer.Western blot was used to determine the expression of proteins relevant to apoptosis.Results:Compared with the health control group,the trunk diameter of portal vein and the thickness of spleen,as well as the hepatic artery pulsation index of clonorchiasis patients increased obviously,the mean blood flow velocity of portal vein(P<0.05 or P<0.01) decreased.The content of total bilirubin and transaminase in plasma increased significantly,but albumin decreased(P<0.05).Levels of TNF-α,IL-6 and IFN-γ increased remarkably,and the level of every factor was significantly different among patients with Child-Pugh Ⅰ,Child-Pugh Ⅱ and Child-Pugh Ⅲ classification of liver function(P<0.05 or P<0.01).With the exacerbation of liver dysfunction,levels of TNF-α,IL-6 and IFN-γ gradually increased(P<0.05).Compared with the healthy control group,the expression quantity of apoptosis protein Fas,FasL,Bax and Caspase-3 increased significantly(P<0.05 or P<0.01),but Bcl-2 decreased(P<0.05).Conclusions:Changes of ultrasonic characteristics and liver dysfunction,caused by liver fluke infection,may be related to that both inflammatory response and apoptosis response have participated in the pathogenic process and liver damage course of clonorchiasis. | Mei Fan Lin Lu Chun Su Mei Xue Ji-Mei Dou Pei Li Han-Qi Feng Yan-Bing Fan | 2016 | Asian Pacific Journal of Tropical Medicine2016，9，7： | 3 |
| 5 | Asymptotic Behaviors for Critical Branching Processes with Immigration显示文摘In this paper, we investigate the asymptotic behaviors of the critical branching process with immigration {Z_n, n ≥ 0}. First we get some estimation for the probability generating function of Zn. Based on it, we get a large deviation for Z_(n+1)/Z_n. Lower and upper deviations for Zn are also studied. As a by-product, an upper deviation for max_(1≤i≤n) Z_i is obtained. | Dou Dou LI Mei ZHANG | 2019 | Acta Mathematica Sinica,English Series2019，35，4： | 3 |
| 6 | Research on Isolation and Clone of Embryonic Stem Cell-Like in Bovine显示文摘Bovine embryonic stem cell would be invaluable for researching the aspect of animal cloning, production transgenic animal and discussion of gene function in vitro. With the object of establishing an effective culture system for isolation and clone of bovine pluripotent stem cell, we cultured bovine embryos and mouse embryos including morula blastula and hatached blastula and obtained animal ICM on Primary marine embryonic fibroblast (Primary murine embryonic fibroblast, PMEF) feeder layer with tissue medium(DMEM supplemented with 15ml/100ml NBS ,0.1μmol/L Na2SeO3, 0. 1mmol/L β-mercaptoethanol, 1 000ng/ml LIF,10 ng/ml IGF, 1mmol/L necessary amino acid and 1mmol/L L-glutamine), then, we obtained mouse ICM and bovine ICM. Moreover, we isolated and cloned the 6 passage bovine ES like cells(12 cell lines) and 9 passage marine ES like cells (52 cell lines) deriving from bovine ICM and murine ICM respectively on the feeder layer of PMEF by disaggregating ICM and ES cell clones of bovine and murine into smaller clumps through digesting with 0. 125g/100ml trypsin and 0.02g/100ml EDTA and scattering with a glass needle. The pluripotency of both murine and bovine ES like cells was identified with morphological character, histochemistry identification, karyotype analysis and differentiation of ES cells in vitro or in vivo. This result showed that bovine embryonic stem cell and murine embryonic stem cell had developmental pluripotency. | AN Li-long, YANG Qi, XIAO Mei, FENG Xiu-Liang, YANG Chun-rong, LEI An-min, GAO Zhi-min, DOU Zhong-ying and QIU Huai( Agriculture College of Zhanjiang Ocean University, Zhanjiang 524008 , P. R . China Key laboratory of AnimalReproductive Endocrinology and Embryo Biotechnology of Agricultural Ministry of China , Northwestern Scienceand Technology University of Agriculture and Forestry, yangling 712100 , P.R. China) | 2002 | Agricultural Sciences in China2002，1，4： | 2 |
| 7 | Performances of hyperbolical and spherical lens imaging systems at millimeter wavelengths 显示文摘 | DOU W | 2002 | Journal of Electromagnetic Waves and Applications2002，16，8： | 1 |
| 8 | Performances of hyperbolical and spherical lens imaging systems at millimeter wavelengths显示文摘 | MEI Z DOU W | 2002 | Journal of Eleetromagn Waves and Applications2002，16，23： | 1 |
| 9 | Study on seawater softening by nanofiltration membrane显示文摘 | Wang Qin Nan Suifei Dou Mei | 2008 | J ChemEng of Chinese Univ2008，22，4： | 1 |
| 10 | Divergent responses of soil carbon and nitrogen pools to short-term nitrogen addition between two plantations in Northeast China显示文摘Nitrogen(N)deposition has a profound influence on forest soil carbon(C)and N pools,but there was no consensus on the responses of different C and N components in different forest types.In this study,a two-year simulated N deposition experiment with four levels of N(NH4NO3)-addition treatments(0,50,100,and 150 kg N/hm^(2)·a)were conducted in Larix gmelinii(LG)and Quercus mongolica(QM)plantation in Northeast China,in order to investigate the C and N pool dynamics under continuously enhanced N deposition.Soil organic carbon(SOC),soil total N(STN)and their active components(readily oxidizable C,ROC;dissolved organic C,DOC;microbial biomass C,MBC,dissolved organic N,DON;microbial biomass N,MBN)of the forest soil were measured monthly from May to October 2017.C and N contents in LG were observed higher than in QM.N addition had no effect on SOC and STN of LG,but significantly increased SOC and STN of QM at low N addition level.Low N addition generally raised active C components(ROC,DOC,and MBC)in both plantations,whereas high N addition did not significantly affect these components,or even decreased ROC in LG soil.Low N addition also increased STN and MBN of QM,while no significant change in STN and MBN of LG was observed.DON was directly affected by N addition and increased significantly with elevated N addition levels.The results indicated that N addition,especially of low rate,might enhance the C sequestration capacity of the forest soils and mitigate climate change. | Yedong Li Bo Wang Sen Dou Haoyue Shen Liyong Mei Yang Zhang Ximing Zeng Yaya Zhang Yameng Pei Haoye Ren Shimin Wu | 2019 | International Journal of Agricultural and Biological Engineering2019，12，6： | 1 |
| 11 | Supplementation with magnesium and vitamin E were more effective than magnesium alone to decrease plasma lipids and blood viscosity in diabetic rats显示文摘 | Mei Dou Ai Guo Ma Qiu Zhen Wang | 2009 | Nutrition Research2009，，7： | 1 |
| 12 | Phonon characteristics and dielectric properties of BaMoO_(4)ceramic显示文摘BaMoO_(4)ceramic was prepared using a conventional solid-sintering method.It was observed that the sample is a pure BaMoO_(4)with a tetragonal scheelite structure by analyzing X-ray diffraction data.Scanning electron microscopy characterized the dense sample with uniform grains.The phonon modes were analyzed by Raman and Far-infrared reflection spectra,and the phonon characteristics were studied.The intrinsic properties of the sample were calculated by the four-parameter semi-quantum model as well as the Clausius-Mosotti&damping equations,and the results were ofεr=9.388 and tanδ=4.760×10^(-4),εr=9.798 and tanδ=6.445×10^(-4),respectively,which agrees well with the experimental values(εr=9.84,tanδ=5.0×10^(-4)).The contributions to dielectric properties of each mode were investigated,and the results indicate that the external mode(Eu)yield greatest contribution to intrinsic permittivity and loss. | En-Cai Xiao Jianzhu Li Jing Wang Chao Xing Mei Guo Hengyang Qiao Qing Wang Ze-Ming Qi Gang Dou Feng Shi | 2018 | Journal of Materiomics2018，4，4： | 1 |
| 13 | The networking technology within smart home system zigbee technology显示文摘 | Niu Dou Yang Mei | 2009 | International Forum on Computer Science Technology and Applications2009，，2： | 1 |
| 14 | Sialylation of TLR2 initiates osteoclast fusion显示文摘The molecular control of osteoclast formation is still not clearly elucidated. Here, we show that a process of cell recognition mediated by Siglec15-TLR2 binding is indispensable and occurs prior to cell fusion in RANKL-mediated osteoclastogenesis. Siglec15 has been shown to regulate osteoclastic bone resorption. However, the receptor for Siglec15 has not been identified, and the signaling mechanism involving Siglec15 in osteoclast function remains unclear. We found that Siglec15 bound sialylated TLR2 as its receptor and that the binding of sialylated TLR2 to Siglec15 in macrophages committed to the osteoclast-lineage initiated cell fusion for osteoclast formation, in which sialic acid was transferred by the sialyltransferase ST3 Gal1. Interestingly, the expression of Siglec15 in macrophages was activated by M-CSF, whereas ST3 Gal1 expression was induced by RANKL. Both Siglec15-specific deletion in macrophages and intrafemoral injection of sialidase abrogated cell recognition and reduced subsequent cell fusion for the formation of osteoclasts, resulting in increased bone formation in mice. Thus, our results reveal that cell recognition mediated by the binding of sialylated TLR2 to Siglec15 initiates cell fusion for osteoclast formation. | Ce Dou Gehua Zhen Yang Dan Mei Wan Nathachit Limjunyawong Xu Cao | 2022 | Bone Research2022，10，2： | 1 |
| 15 | Remarkable improvement of cyclic stability in Li-O2 batteries using ruthenocene as a redox mediator显示文摘Nonaqueous Li-O2 batteries attract attention for their theoretical specific energy density.However,due to the difficulty of decomposition of Li2 O2,Li-O2 batteries have high charge overpotential and poor cycling life.So all kinds of catalysts have been studied on the cathode.Compared to heterogeneous solid catalysts,soluble catalysts achieve faster and more effective transport of electrons by reversible redox pairs.Here,we first report ruthenocene(Ruc) as a mobile redox mediator in a Li-O2 battery.0.01 mol/L Ruc in the electrolyte effectively reduces the charging voltage by 610 mV.Additionally,Ruc greatly increases the cycling life by four-fold(up to 83 cycles) with a simple ketjen black(KB) cathode.The results of SEM,XPS and XRD confirm that less discharge product residue accumulated after recharge.To verify the reaction mechanisms of the mediato r,free energy profiles of the possible reaction pathways based on DFT are provided. | Cuicui Zhu Yiping Wang Ling Shuai Yizhao Tang Ming Qiu Jian Xie Jia Liu Wen Wen Hengquan Chen Suifei Nan Mei Dou Qinggang He | 2020 | Chinese Chemical Letters2020，31，7： | 1 |
| 16 | Research progress on insulin resistance causes and mechanism 显示文摘 | Dou Mei Ma Ai-guo | 2009 | Foreign Medical Sciences Section Hygiene2009，36，3： | 1 |
| 17 | SYNTHESIS AND STRUCTURE OF Cu(Ⅱ)COMPLEXES BY OXIDATIVE ADDITION OF DIBENZOYL PEROXIDE ON METALLIC COPPER显示文摘Dibenzoyl peroxide undergoes oxidative addition on metallic copperpowder with 2,2′-bipyridine(or imidazole)in a mixed solvent(methanol andtetrahydrofuran),and affords the Cu(Ⅱ)complexes-[Cu(Ce(C6H5COO)2(2,2’-bipy)]H2O(1)and[Cu(C6H5COO)2(C3H4N2)2](2).The structure was solved by direct methodsand Fourier synthesis.C24H20N2O5Cu (1),Mr=479.78,space group P2(1)/c,a=6.986(7),b=18.833(I),c=17.021(3),α=γ=90°,Z=4,V=2218.13,Dc=1.443g/cm\+3,R=0.055Rw=0.062.Complex(2),C20H18N4O4Cu(2),Mr=441.74,space group P2(1)/n,a=8.699(4),b=9.840(6),c=12.399(5),α=γ=90°,β=100.8°,Z=4,V=1010.93,Dc=1.654g/cm\+3,R=0.055,Rw=0.062. | Rui Na YANG Dong Mei WANG Dou Man JIN Henan Institute of Chemistry,Zhengzhou 450003Hua Qin WANG Yao YANG Nanjing University,Nanjing 210008 | 1993 | Chinese Chemical Letters1993，4，2： | 0 |
| 18 | The Massive Expansion and Spatial Transformation of Potentially Contaminated Land Across China in 1990–2020 Observed from Remote Sensing and Big-data显示文摘Identifying and monitoring the spatiotemporal patterns of potentially contaminated land(PCL) in China is a key concern of ecological governance. However, the dynamics of PCL’s expansion remain unclear nationwide. Integrating high-resolution remote sensing images, a land-use/cover change database, crawler data from websites, and other multisource data, we produced a new dataset of China’s PCL in 1990, 2000, 2010, and 2020 using data fusion technology. Then we analyzed the spatiotemporal patterns of China’s PCL from 1990 to 2020. Our study shows that the acquired vector dataset of China’s PCL is of high quality and reliability, with an overall accuracy of 93.21%. The area of China’s PCL has kept growing for the past 30 years, and the growth rate was especially rapid during2000–2010, 2.32 and 6.13 times as rapid as that during 1990–2000 and 2010–2020, respectively. PCL has also been trending toward higher aggregation over markedly enlarged areas and has transferred progressively from north and southeast of China to northwest and southwest of China and Qinghai-Tibet Plateau. The patterns of China’s PCL have been driven by the joint factors of policies, mineral resources, economy, and others, among which policies and the economy have contributed more prominently to the long-term transition.Our study promotes the access to high-quality spatial data of PCL to facilitate environmental governance of mine wastes, pollution and land management. | DOU Yinyin GUO Changqing KUANG Wenhui CHI Wenfeng LEI Mei | 2022 | Chinese Geographical Science2022，32，5： | 0 |
| 19 | Limit Theorems for Critical Galton-Watson Processes with Immigration Stopped at Zero显示文摘In this paper,we consider a critical Galton-Watson branching process with immigration stopped at zero W.Some precise estimation on the probability generating function of the n-th population are obtained,and the tail probability of the life period of W is studied.Based on above results,two conditional limit theorems for W are established. | Dou Dou LI Mei ZHANG Xian Yu ZHANG | 2024 | Acta Mathematica Sinica,English Series2024，40，2： | 0 |
| 20 | Research on Growth Behavior of Embryos for Bovine and Murine on Primary Murine Embryos Fibroblast Cell Feeder Layer显示文摘The difference in growth behavior between bovine embryos and murine embryos was studied on PMEF(primary murine embryos fibroblast)feeder layer. The results showed as follows: With embryos having attached, bovine embryonic trophoblast formed a transparent membranous structure covering on inner cell mass (ICM), however, murine embryonic trophoblast formed disc structure. Bovine embryos formed four kinds of ICM colonies with different morphology including the mass-like, the net-like, the stream-like and the mixture-like colonies. Compared with Murine ICM, the bovine ICM grew more fast. So, the bovine ICM was passaged at first after a culture of approximately 5 - 6 days in vitro, but murine ICM was passaged at first after an attachment of 3 - 4 days on PMEF feeder layer. The mixture colonies of bovine ICM differentiated very early, while the others differentiated very late. Most ICM-like mass of Bovine grew in a defined spot, but bovine ICMs like stream and ICMs like net proliferated fast and dispersed quickly. We found that the single blastomeres derived from late bovine morula and late murine morula formed sub-blastophere; moreover, the bovine ICM cell would differentiate rapidly if the trophoblast was removed. | AN Li-long, XIAO Mei, FENG Xiu-Liang, DOU Zhong-ying, QIU Huai,YANG Qi, LEI An-min, YANG Chun-rong and GAO Zhi-min( Agriculture College of Zhanjiang Ocean University, Zhanjiang 524008 , China Key Laboratory of Animal Reproductive Endocrinology and Embryo Biotechnology of Agricultural Ministry of China , Northwestern Science and Technology University ofAgriculture and Forestry, Yangling 712100 , P.R. China) | 2002 | Agricultural Sciences in China2002，1，4： | 0 |
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