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| 1 | A Chromosome-Scale Genome Assembly of Paper Mulberry(Bmussonetia papyrifera)Provides New Insights into Its Forage and Papermaking Usage显示文摘Paper mulberry(Broussonetia papyrifera)is a well-known woody tree historically used for Cai Lun papermaking,one of the four great inventions of ancient China.More recently,Paper mulberry has also been used as forage to address the shortage of feedstuff because of its digestible crude fiber and high protein contents.In this study,we obtained a chromosome-scale genome assembly for Paper mulberry using integrated approaches,including Illumina and PacBio sequencing platform as well as Hi-C,optical,and genetic maps.The assembled Paper mulberry genome consists of 386.83 Mb,which is close to the estimated size,and 99.25%(383.93 Mb)of the assembly was assigned to 13 pseudochromosomes.Comparative genomic analysis revealed the expansion and contraction in the flavonoid and lignin biosynthetic gene families,respectively,accounting for the enhanced flavonoid and decreased lignin biosynthesis in Paper mulberry.Moreover,the increased ratio of syringyl-lignin to guaiacyl-lignin in Paper mulberry underscores its suitability for use in medicine,forage,papermaking,and barkcloth making.We also identified the rootassociated microbiota of Paper mulberry and found that Pseudomonas and Rhizobia were enriched in its roots and may provide the source of nitrogen for its stems and leaves via symbiotic nitrogen fixation.Collectively,these results suggest that Paper mulberry might have undergone adaptive evolution and recruited nitrogen-fixing microbes to promote growth by enhancing flavonoid production and altering lignin monomer composition.Our study provides significant insights into genetic basis of the usefulness of Paper mulberry in papermaking and barkcloth making,and as forage.These insights will facilitate further domestication and selection as well as industrial utilization of Paper mulberry worldwide. | Xianjun Peng Hui Liu Peilin Chen Feng Tang Yanmin Hu Fenfen Wang Zhi Pi Meiling Zhao Naizhi Chen Hui Chen Xiaokang Zhang Xueqing Yan Min Liu Xiaojun Fu Guofeng Zhao Pu Yao Lili Wang He Dai Xuming Li Wei Xiong Wencai Xu Hongkun Zheng Haiyan Yu Shihua Shen | 2019 | Molecular Plant2019，12，5： | 18 |
| 2 | In vitro Anthocyanin Induction and Metabolite Analysis in Malus spectabilis Leaves Under Low Nitrogen Conditions显示文摘Anthocyanins are the most widely produced secondary metabolites in plants,and they play an important role in plant growth and reproduction.The nitrogen source is an important factor affecting anthocyanin production,but the nitrogen concentrations on metabolism and the underlying genetic basis remain unclear.In this study,in vitro anthocyanin induction was conducted on Malus spectabilis.The leaf explants were cultivated in media containing different nitrogen concentrations.The results suggested that when the nitrogen contents decreased in limit,the color of leaf explants turned from green to red,and increased anthocyanin accumulation led to a change in phenotype.Furthermore,the content of other flavonoids,such as dihydroquercetin,epicatechin,and catechin,increased under low nitrogen conditions.The transcription levels of the general flavonoid pathway genes,from phenylalanine ammonia lyase(PAL)to anthocyanidin synthase(ANS),were associated with the concentration of corresponding flavonoid compounds and phenotype changes.In particular,the expression level of ANS increased substantially under a low nitrogen treatment,which was significantly and positively correlated with the anthocyanin levels(R2=0.72,P<0.05).The increased expression patterns of anthocyanin pathway genes were similar to that of the transcription factor MYB10.We further verified MYB10 played an important role in the anthocyanin pathway in leaves of Malus spectabilis.These results suggested that we can improve the desirable ornamental plant phenotypes by controlling nitrogen content.This process may offer clues to further development of new agricultural practices. | Jiaxin Meng Yan Gao Meiling Han Pengyuan Liu Chen Yang Ting Shen Houhua Li | 2020 | Horticultural Plant Journal2020，6，5： | 13 |
| 3 | Ni(OH)_(2) nanoparticles encapsulated in conductive nanowire array for high-performance alkaline seawater oxidation显示文摘Design and development of high-efficiency and durable oxygen evolution reaction(OER)electrocatalysts is crucial for hydrogen production from seawater splitting.Herein,we report the in situ electrochemical conversion of a nanoarray of Ni(TCNQ)2(TCNQ=tetracyanoquinodimethane)on graphite paper into Ni(OH)_(2) nanoparticles confined in a conductive TCNQ nanoarray(Ni(OH)_(2)-TCNQ/GP)by anode oxidation.The Ni(OH)_(2)-TCNQ/GP exhibits high OER performance and demands overpotentials of 340 and 382 mV to deliver 100 mA·cm^(−2) in alkaline freshwater and alkaline seawater,respectively.Meanwhile,the Ni(OH)_(2)-TCNQ/GP also demonstrates steady long-term electrochemical durability for at least 80 h under alkaline seawater. | Longcheng Zhang Jiaqian Wang Pengyu Liu Jie Liang Yongsong Luo Guanwei Cui Bo Tang Qian Liu Xuedong Yan Haigang Hao Meiling Liu Rui Gao Xuping Sun | 2022 | Nano Research2022，15，7： | 5 |
| 4 | MRI结合数字化钼靶诊断乳腺癌的临床研究(英文)显示文摘目的将在乳癌的察觉和诊断比较数字早期胸部肿瘤Ⅹ射线测定法和 MRI 的有效性并且象二的联合一样估计这些形式的价值。有外科和病理的 57 个病人证明了胸损害的方法(恶意, n = 32;良性, n = 46 ) 经历了数字早期胸部肿瘤Ⅹ射线测定法和 MRI,脉搏序列包括了 T 1 WI, T 2 WI,弥漫的加权的成像(DWI ) ,和在外科前的动态提高对比的 MRI。这二种形式的结果,所有损害根据 BI-RADS 分类被分类进 5 个组,并且成像调查结果被相关到组织病理学说。每种形式的敏感以及二的联合是计算的。这 78 乳房损害的结果,敏感为数字早期胸部肿瘤Ⅹ射线测定法是 78.13%%(25/32 ) 并且 93.75%(30/32 ) 为 MRI (P >
0.05 ) 。特性是 73.91%%(34/46 ) 并且 89.13%(41/46 ) 因此(P <
0.05 ) ,他们俩出现了统计差别。当这二种形式在联合被使用,敏感和特性分别地是 98.63% 和 97.16% 。在有 MRI 的联合的结论数字早期胸部肿瘤Ⅹ射线测定法在乳癌,敏感和特性的诊断是有用的当时,被提高与单个形式的相比。 | Fang Ye Mengsu Zeng Fuhua Yan Wei Feng Meiling Zhou Renchen Li Wenfang Chen | 2008 | The Chinese-German Journal of Clinical Oncology2008，7，5： | 3 |
| 5 | The potential value of microRNA-4463 in the prognosis evaluation in hepatocellular carcinoma显示文摘The purpose of this study is to measure the expression of microRNA-4463 and microRNA-6087 between normal persons and patients with hepatocellular carcinoma(HCC),and to clarify the meaning of them in the prognosis evaluation in HCC.Forty-five samples from healthy people and patients,who had been diagnosed with hepatocellular carcinoma before any treatment,were collected to study respectively.Real-time PCR was used to detect the expression of miRNA-4463 and miRNA-6087 in the serum of control group and hepatocellular carcinoma patients.The expression of miR-4463 in the serum of HCC patients was significantly higher than that in control group(P<0.05),and the expression level was independent of gender,tumor size,cell types,stages,alanine aminotransferase(ALT),aspartate aminotransferase(AST),total bilirubin(TBIL)and HBsAg status(P>0.05).But there was a significant difference of different level of AFP in HCC(P<0.05),and the difference between the group of AFP lower than 400 ug/l and the control group is statistically significant(P<0.05).Besides,the survival time had showed a significant difference at the high and low expression levels(P<0.05).But the expression level of miRNA-6087 was no difference in HCC and control group.The disorder of miRNA-4463 occurred in HCC,even the AFP level doesn’t rises.What’s more,patients who get the high level of miRNA-4463 seem to have a shorter survival time.And it contributes great to the prognostic evaluation.This is the first study to illustrate the potential significance of miRNA-4463 in the prognosis in HCC. | Tian Hu Jincheng Li Chuhong Zhang Xiu lv Sai Li Sha He Hanxing Yan Yixi Tan Mingsheng Lei Meiling Wen Jianhong Zuo | 2017 | Genes & Diseases2017，4，2： | 3 |
| 6 | The effects of altered BMP4 signaling in first branchialarch- derived murine embryonic orofacial tissues显示文摘The first branchial arch(BA1),which is derived from cranial neural crest(CNC)cells,gives rise to various orofacial tissues.Cre mice are widely used for the determination of CNC and exploration of gene functions in orofacial development.However,there is a lack of Cre mice specifically marked BA1’s cells.Pax2-Cre allele was previously generated and has been widely used in the field of inner ear development.Here,by compounding Pax2-Cre and R26R-mTmG mice,we found a specific expression pattern of Pax2^(+)cells that marked BA1’s mesenchymal cells and the BA1-derivatives.Compared to Pax2-Cre;R26R-mTmG allele,GFP^(+)cells were abundantly found both in BA1 and second branchial arch in Wnt1-Cre;R26R-mTmG mice.As BMP4 signaling is required for orofacial development,we over-activated Bmp4 by using Pax2-Cre;pMes-BMP4 strain.Interestingly,our results showed bilateral hyperplasia between the upper and lower teeth.We also compare the phenotypes of Wnt1-Cre;pMes-BMP4 and Pax2-Cre;pMes-BMP4 strains and found severe deformation of molar buds,palate,and maxilla-mandibular bony structures in Wnt1-Cre;pMes-BMP4 mice;however,the morphology of these orofacial organs were comparable between controls and Pax2-Cre;pMes-BMP4 mice except for bilateral hyperplastic tissues.We further explore the properties of the hyperplastic tissue and found it is not derived from Runx2^(+)cells but expresses Msx1,and probably caused by abnormal cell proliferation and altered expression pattern of p-Smad1/5/8.In sum,our findings suggest altering BMP4 signaling in BA1-specific cell lineage may lead to unique phenotypes in orofacial regions,further hinting that Pax2-Cre mice could be a new model for genetic manipulation of BA1-derived organogenesis in the orofacial region. | Jue Xu Meiling Chen Yanan Yan Qiaoxue Zhao Meiying Shao Zhen Huang | 2021 | International Journal of Oral Science2021，13，4： | 3 |
| 7 | The role of hypoxia-inducible factors in tumor angiogenesis and cell metabolism显示文摘Hypoxia-inducible factor(HIF)is a main heterodimeric transcription factor that regulates the cellular adaptive response to hypoxia by stimulating the transcription of a series of hypoxia-inducible genes.HIF is frequently upregulated in solid tumors,and the overexpression of HIF can promote tumor progression or aggressiveness by blood vessel architecture and altering cellular metabolism.In this review,we focused on the pivotal role of HIF in tumor angiogenesis and energy metabolism.Furthermore,we also emphasized the possibility of HIF pathway as a potential therapeutic target in cancer. | Xiu Lv Jincheng Li Chuhong Zhang Tian Hu Sai Li Sha He Hanxing Yan Yixi Tan Mingsheng Lei Meiling Wen Jianhong Zuo | 2017 | Genes & Diseases2017，4，1： | 3 |
| 8 | Polycyclic aromatic hydrocarbons (PAHs) in soils and vegetation near an e-waste recycling site in South China: Concentration, distribution, source, and risk assessment显示文摘 | Yan Wang Zhongjing Tian Haolin Zhu Zhineng Cheng Meiling Kang Chunling Luo Jun Li Gan Zhang | 2012 | Science of the Total Environment2012，，： | 1 |
| 9 | Angiogenesis is a link between atherosclerosis and tumorigenesis: Role of LOX-I显示文摘 | Jiang Junlin Yan Meiling Jawahar L | 2011 | Cardiovasc Drugs Ther2011，25，5： | 1 |
| 10 | Simultaneous direct analysis of benzimidazole fungicides and relevant metabolites in agricultural products based on multifunction dispersive solid-phase extraction and liquid chromatography–mass spectrometry显示文摘 | Bin Guo Zhiqiang Huang Meiling Wang Xiaoying Wang Ying Zhang Bo Chen Yongjun Li Hongfei Yan Shouzhuo Yao | 2010 | Journal of Chromatography A2010，，29： | 1 |
| 11 | A multicentre randomised trial comparing weekly paclitaxel<ce:hsp sp='0.25'/>+<ce:hsp sp='0.25'/>S-1 with weekly paclitaxel<ce:hsp sp='0.25'/>+<ce:hsp sp='0.25'/>5-fluorouracil for patients with advanced gastric cancer显示文摘 | Dingzhi Huang Yi Ba Jianping Xiong Nong Xu Zhao Yan Zhixiang Zhuang Zhuang Yu Huiping Wan Yang Zhang Ting Deng Rongsheng Zheng Zengqing Guo Chunhong Hu Meiling Wang Zhonghe Yu Yang Yao Jichang Meng | 2013 | European Journal of Cancer2013，，： | 1 |
| 12 | CRISPR/Cas9 screening identifies a kinetochore-microtubule dependent mechanism for Aurora-A inhibitor resistance in breast cancer显示文摘Background:Overexpression of Aurora-A(AURKA)is a feature of breast cancer and associates with adverse prognosis.The selective Aurora-A inhibitor alisertib(MLN8237)has recently demonstrated promising antitumor responses as a single agent in various cancer types but its phase III clinical trial was reported as a failure since MLN8237 did not show an apparent effect in prolonging the survival of patients.Thus,identification of potential targets that could enhance the activity of MLN8237 would provide a rationale for drug combination to achieve better therapeutic outcome.Methods:Here,we conducted a systematic synthetic lethality CRISPR/Cas9 screening of 507 kinases using MLN8237 in breast cancer cells and identified a number of targetable kinases that displayed synthetic lethality interactions with MLN8237.Then,we performed competitive growth assays,colony formation assays,cell viability assays,apoptosis assays,and xenograft murine model to evaluate the synergistic therapeutic effects of Haspin(GSG2)depletion or inhibition with MLN8237.For mechanistic studies,immunofluorescence was used to detect the state of microtubules and the localization of Aurora-B and mitotic centromere-associated kinesin(MCAK).Results:Among the hits,we observed that Haspin depletion or inhibition marginally inhibited breast cancer cell growth but could substantially enhance the killing effects of MLN8237.Mechanistic studies showed that co-treatment with Aurora-A and Haspin inhibitors abolished the recruitment of Aurora-B and mitotic centromere-associated kinesin(MCAK)to centromeres which were associated with excessive microtubule depolymerization,kinetochore-microtubule(KT-MT)attachment failure,and severe mitotic catastrophe.We further showed that the combination of MLN8237 and the Haspin inhibitor CHR-6494 synergistically reduced breast cancer cell viability and significantly inhibited both in vitro and in vivo tumor growth.Conclusions:These findings establish Haspin as a synthetic lethal target and demonstrate CHR-6494 as a potential combinational drug for promoting the therapeutic effects of MLN8237 on breast cancer. | Ailin Chen Shijun Wen Fang Liu Zijian Zhang Meiling Liu Yuanzhong Wu Bin He Min Yan Tiebang Kang Eric W-F Lam Zifeng Wang Quentin Liu | 2021 | Cancer Communications2021，41，2： | 1 |
| 13 | A mini-review on pharmacological effects of ginsenoside Rb3,a marked saponin from Panax genus显示文摘Ginsenoside Rb3(G-Rb3)is one of the primary active compounds isolated from Panax ginseng Meyer,which belongs to protopanaxadiol ginsenosides(PPD).Based on the structure-activity relationship(SAR)of ginsenosides,the pentose structure of G-Rb3 limited itself to possess more pharmacological activity to a certain extent.However,pharmacokinetics show that G-Rb3 is processed through deglycosylation in the intestinal tract and converted into more active rare saponins,such as Compound K,F2,etc.A series of studies focused on neuroprotection and the cardiovascular system demonstrating its therapeutic potentials,which was achieved by diminishing oxidative stress and apoptosis.Therefore,more systematic and in-depth studies are needed to complete the pharmaceutical value and to promote its clinical applications.This article highlights the multiple pharmacological effects and mechanisms of G-Rb3 and prospects for its development. | WEI LI YUEYANG DUAN XIAOTONG YAN XIANGXIANG LIU MEILING FAN ZI WANG | 2022 | BIOCELL2022，46，6： | 1 |
| 14 | Neurodegeneration-associated FUS is a novel regulator of circadian gene expression显示文摘Background:Circadian rhythms are oscillating physiological and behavioral changes governed by an internal molecular clock,and dysfunctions in circadian rhythms have been associated with ageing and various neurodegenerative diseases.However,the evidence directly connecting the neurodegeneration-associated proteins to circadian control at the molecular level remains sparse.Methods:Using meta-analysis,synchronized animals and cell lines,cells and tissues from FUS R521C knock-in rats,we examined the role of FUS in circadian gene expression regulation.Results:We found that FUS,an oscillating expressed nuclear protein implicated in the pathogenesis of amyotrophic lateral sclerosis(ALS)and frontotemporal dementia(FTD),exerted a novel feedback route to regulate circadian gene expression.Nr1d1-encoded core circadian protein REV-ERBαbound the Fus promoter and regulated the expression of Fus.Meanwhile,FUS was in the same complex as PER/CRY,and repressed the expression of E box-containing core circadian genes,such as Per2,by mediating the promoter occupancy of PSF-HDAC1.Remarkably,a common pathogenic mutant FUS(R521C)showed increased binding to PSF,and caused decreased expression of Per2.Conclusions:Therefore,we have demonstrated FUS as a modulator of circadian gene expression,and provided novel mechanistic insights into the mutual influence between circadian control and neurodegeneration-associated proteins. | Xin Jiang Tao Zhang Haifang Wang Tao Wang Meiling Qin Puhua Bao Ruiqi Wang Yuwei Liu Hung-Chun Chang Jun Yan Jin Xu | 2018 | Translational Neurodegeneration2018，7，1： | 1 |
| 15 | A Liquid Chromatographic Method for Simultaneous Determination of Amoxicillin Sodium and Sulbactam Sodium in a Combination Formulation显示文摘 | Wang Peng Qi Meiling Sun Yan | | 0，，03： | 1 |
| 16 | Burning magnesium in carbon dioxide for highly effective phosphate removal显示文摘Magnesium oxide was found to have high-phosphate-affinity as an effective component to enhance the phosphate removal ability of common adsorbent materials.However,the currently prepared MgO-based hybrid adsorbents by conventional methods still suffer from the limited low loading of MgO and inferior removal performances,much far away from practical application.In this study,an ingenious carbon coated MgO nanocomposite is designed by directly burning magnesium in CO_(2),a well-known textbook reaction.X-ray diffraction analysis,scanning electron microscope and aberration-corrected high-resolution transmission electron microscope demonstrate the sample is well prepared.Consequently,the high content of nanosized MgO combined with defect-rich carbon layer brings unprecedented phosphate removal capacity of 1135.0 mg/g,removal rate of 99% and benign compatibility with coexisting anions and solution pH.Furthermore,the removal mechanism is also investigated in detail by characterizing the sample before and after adsorption. | Ying Yao Lei Yu Meiling Wang Alvin Dai Yan Zhang Qiubo Guo Yulin Lin JianguoWen Feng Wu Xiulei Ji Jun Lu | 2021 | Carbon Energy2021，3，2： | 0 |
| 17 | SnoRNP is essential for thermospermine-mediated development in Arabidopsis thaliana显示文摘Polyamines have been discovered for hundreds of years and once considered as a class of phytohormones.Polyamines play critical roles in a range of developmental processes.However,the molecular mechanisms of polyamine signaling pathways remain poorly understood.Here,we measured the contents of main types of polyamines,and found that endogenous level of thermospermine(T-Spm)in Arabidopsis thaliana is comparable to those of classic phytohormones and is significantly lower than those of putrescine(Put),spermidine(Spd),and spermine(Spm).We further found a nodule-like structure around the junction area connecting the shoot and root of the T-Spm biosynthetic mutant acl5 and obtained more than 50 suppressors of acl5 nodule structure(san)through suppressor screening.An in-depth study of two san suppressors revealed that NAP57 and NOP56,core components of box H/ACA and C/D snoRNPs,were essential for T-Spm-mediated nodule-like structure formation and plant height.Furthermore,analyses of rRNA modifications showed that the overall levels of pseudouridylation and 2′-O-methylation were compromised in san1 and san2 respectively.Taken together,these results establish a strong genetic relationship between rRNA modification and T-Spm-mediated growth and development,which was previously undiscovered in all organisms. | Xilong Li Zongyun Yan Meiling Zhang Jiayin Wang Peiyong Xin Shujing Cheng Liquan Kou Xiaoting Zhang Songlin Wu Jinfang Chu Chengqi Yi Keqiong Ye Bing Wang Jiayang Li | 2023 | Science China(Life Sciences)2023，66，1： | 0 |
| 18 | An efficient method and system for simultaneously measuring the performance of vast scintillating materials显示文摘A method and system for automatically and simultaneously measuring the light output of multiple scintillators,or each scintillating unit of an array,were developed.Using a large area flat panel PSPMT H8500,the light output and energy resolution were obtained automatically by comparing with reference scintillators or array using an Energy Table,Look-up Table and energy spectrum data.The aim of developing an efficient performance evaluation of scintillators was achieved.Using the method,a scintillator performance testing system was set up and six LYSO crystals and a 3×3 LYSO array were measured.The results showed that the light output and energy resolution were accurately measured automatically.The deviation of repeat measurements for the same sample was not more than 2%,and the nonlinear deviation of the system was not more than 3%.The system is suitable for measuring the performance of crystals,especially where the mass measurement of crystals and arrays is required. | CHEN Yan ZHANG Zhiming LI Daowu SHUAI Lei HUANG Xianchao TANG Haohui LI Ting ZHUANG Kai WANG yingjie LIU Yantao ZHANG Yiwen CHAI Pei LIU Shuangquan ZHU Meiling WANG Baoyi WEI Long | 2012 | Nuclear Science and Techniques2012，23，3： | 0 |
| 19 | Viral receptor profiles of masked palm civet revealed by single-cell transcriptomics显示文摘Civets are small mammals belonging to the family Viverridae.The masked palm civets(Paguma larvata)served as an intermediate host in the bat-to-human transmission of severe acute respiratory syndrome coronavirus(SARS-Co V)in 2003(Guan et al.,2003).Because of their unique role in the SARS outbreak,civets were suspected as a potential intermediate host of SARS-Co V-2. | Peiwen Ding Haoyu Wang Jiacheng Zhu Fuyu An Jinqian Xu Xiangning Ding Lihua Luo Weiying Wu Qiuyu Qin Yanan Wei Wandong Zhao Zhiyuan Lv Haimeng Li Yixin Zhu Meiling Li Wensheng Zhang Yanan Zhang Zhihua Ou Huan Liu Yan Hua | 2022 | Journal of Genetics and Genomics2022，49，11： | 0 |
| 20 | From surface loading to precise confinement of polyoxometalates for electrochemical energy storage显示文摘Because of abundant redox activity,broad tunability,and specific atomic structure,polyoxometalates(POMs or POM)clusters have attracted burgeoning interests in electrochemical especially energy stor-age fields.Nevertheless,due to the high solubility and fully oxidized state,they often suffer from elec-trically insulation as well as chemical and electrochemical instability.Traditional noncovalent loading or covalent grafting of POMs on conductive substrates have been successfully performed to overcome this problem.However,severe shedding or agglomeration of POMs arising from weak interactions with substrates or excessive entrapment or weak destruction in conductive supports cause significantly re-duced availability and stability.To this end,precise confinement of POMs into conductive supports has been tried to improve their dispersibility and stability.Herein,recent progress of PoMs from surface loading to precise confinement in the electrochemistry energy storage field is reviewed.Firstly,we il-lustrate the typical non-confinement methods(viz.covalent and non-covalent)for supported POMs in energy storage applications.Secondly,different strategies for precise confinement of PoMs in organic and inorganic materials for related applications are also discussed.Finally,future research directions and opportunities for confined POMs,and derived ultrafine nanostructures are also proposed.This re-view seeks to point out future research directions of supported PoMs in the electrochemistry-related fields. | Chongji Wang Yanhui Song Wenhua Cong Yuanyuan Yan Meiling Wang Jiadong Zhou | 2023 | Chinese Chemical Letters2023，34，12： | 0 |
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