|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Application of bioactive hydrogels combined with dental pulp stem cells for the repair of large gap peripheral nerve injuries显示文摘Due to the limitations in autogenous nerve grafting or Schwann cell transplantation,large gap peripheral nerve injuries require a bridging strategy supported by nerve conduit.Cell based therapies provide a novel treatment for peripheral nerve injuries.In this study,we first experimented an optimal scaffold material synthesis protocol,from where we selected the 10%GFD formula(10%GelMA hydrogel,recombinant human basic fibroblast growth factor and dental pulp stem cells(DPSCs))to fill a cellulose/soy protein isolate composite membrane(CSM)tube to construct a third generation of nerve regeneration conduit,CSM-GFD.Then this CSM-GFD conduit was applied to repair a 15-mm long defect of sciatic nerve in a rat model.After 12 week post implant surgery,at histologic level,we found CSM-GFD conduit could regenerate nerve tissue like neuron and Schwann like nerve cells and myelinated nerve fibers.At physical level,CSM-GFD achieved functional recovery assessed by a sciatic functional index study.In both levels,CSM-GFD performed like what gold standard,the nerve autograft,could do.Further,we unveiled that almost all newly formed nerve tissue at defect site was originated from the direct differentiation of exogeneous DPSCs in CSM-GFD.In conclusion,we claimed that this third-generation nerve regeneration conduit,CSM-GFD,could be a promising tissue engineering approach to replace the conventional nerve autograft to treat the large gap defect in peripheral nerve injuries. | Lihua Luo Yan He Ling Jin Yanni Zhang Fernando P.Guastaldi Abdullkhaleg A.Albashari Fengting Hu Xiaoyan Wang Lei Wang Jian Xiao Lingli Li Jianming Wang Akon Higuchi Qingsong Ye | 2021 | Bioactive Materials2021，6，3： | 11 |
| 2 | Integrated genomic analysis identifies deregulated JAK/STAT-MYC-biosynthesis axis in aggressive NK-cell leukemia显示文摘好攻击的 NK 房间白血病(ANKL ) 是在从亚洲的人之中是更流行的 NK 房间瘤的一种稀罕形式并且中央并且南美洲。病人们通常在天以内死到月,甚至在收到迅速的治疗学的管理以后。这里,我们由集成整个染色体执行了 ANKL 的第一全面研究, transcriptome 并且定序指向,象功能的试金一样的 cytokine 数组。在 JAK-STAT 小径的变化在 48% 被识别(14/29 ) ANKL 病人,当细胞外的 STAT3 激发器 IL10 被 56 褶层的一般水准提高时(P < 0.0001 ) 在检查的所有病人的血浆。另外的经常变异的基因包括的 TP53 (34%) , TET2 (28%) , CREBBP (21%) 和 MLL2 (21%) 。耐心的 NK 白血病房间在多重新陈代谢的小径显示出 STAT3 phosphorylation, MYC 表示和 transcriptional 活动的突出的激活。机能上地, STAT3 激活和 MYC 表示为 ANKL 房间的增长和幸存是批评的。发信号的 STAT 调整了发信号的 MYC 抄写节目,和两 STAT, MYC 抄写被要求维持核苷酸合成和 glycolysis 的激活。一起, JAK-STAT 小径在 ANKL 为 genomic 改变和 IL10 刺激代表一个主要目标。这最新发现的 JAK/STAT-MYC-biosynthesis 轴可以在对待白血病的这种子类型为新奇治疗学的策略的发展提供机会。 | Liang Huang Dan Liu Na Wang Shaoping Ling Yuting Tang Jun Wu Lingtong Hao Hui Luo Xuelian Hu Lingshuang Sheng Lijun Zhu Di Wang Yi Luo Zhen Shang Min Xiao Xia Mao Kuangguo Zhou Lihua Cao Lili Dong Xinchang Zheng Pinpin Sui Jianlin He Shanlan Mo Jin Yan Qilin Ao Lugui Qiu Hongsheng Zhou Qifa Liu Hongyu Zhang Jianyong Li Jie Jin Li Fu Weili Zhao Jieping Chen Xin Du Guoliang Qing Hudan Liu Xin Liu Gang Huang Ding Ma Jianfeng Zhou Qian-fei Wang | 2018 | Cell Research2018，28，2： | 5 |
| 3 | Responses of ammonia-oxidizing microorganisms to biochar and compost amendments of heavy metals-polluted soil显示文摘Heavy metal pollution affects soil ecological function.Biochar and compost can effectively remediate heavy metals and increase soil nutrients.The effects and mechanisms of biochar and compost amendments on soil nitrogen cycle function in heavy-metal contaminated soils are not fully understood.This study examined how biochar,compost,and their integrated use affected ammonia-oxidizing microorganisms in heavy metal polluted soil.Quantitative PCR was used to determine the abundance of ammonia-oxidizing archaea(AOA)and bacteria(AOB).Ammonia monooxygenase(AMO)activity was evaluated by the enzymelinked immunosorbent assay.Results showed that compost rather than biochar improved nitrogen conversion in soil.Biochar,compost,or their integrated application significantly reduced the effective Zn and Cd speciation.Adding compost obviously increased As and Cu effective speciation,bacterial 16 S rRNA abundance,and AMO activity.AOB,stimulated by compost addition,was significantly more abundant than AOA throughout remediation.Correlation analysis showed that AOB abundance positively correlated with NO_(3)^(-)-N(r=0.830,P<0.01),and that AMO activity had significant correlation with EC(r=-0.908,P<0.01)and water-soluble carbon(r=-0.868,P<0.01).Those seem to be the most vital factors affecting AOB community and their function in heavy metal-polluted soil remediated by biochar and compost. | Mingyue Li Jiachao Zhang Xiao Yang Yaoyu Zhou Lihua Zhang Yuan Yang Lin Luo Qingyun Yan | 2021 | Journal of Environmental Sciences2021，33，4： | 5 |
| 4 | Antiferromagnetic element Mn modified PtCo truncated octahedral nanoparticles with enhanced activity and durability for direct methanol fuel cells显示文摘Pt-based magnetic nano catalysts are one of the most suitable cand idates for electrocatalytic materials due to their high electrochemistry activity and retrievability.Unfortunately,the inferior durability prevents them from being scaled-up,limiting their commercial applications.Herein,an antiferromagnetic element Mn was introduced into PtCo nanostructured alloy to synthesize uniform Mn-PtCo truncated octahedral nanoparticles(TONPs)by one-pot method.Our results show that Mn can tune the blocking temperature of Mn-PtCo TONPs due to its an tiferromag netism.At low temperatures,Mn-PtCo TONPs are ferromag netic,and the coercivity in creases gradually with in creasi ng Mn contents.At room temperature,the Mn-PtCo TONPs display superparamag netic behavior,which is greatly helpful for in dustrial recycling.Mn doping can not only modify the electronic structure of PtCo TONPs but also enhanee electrocatalytic performance for methanol oxidation reaction.The maximum specific activity of Mn-PtCo-3 reaches 8.1 A`m^-2,3.6 times of commercial Pt/C(2.2 A·m^-2)and 1.4 times of PtCo TONPs(5.6 A`m^-2),respectively.The mass activity decreases by only 30%after 2,000 cycles,while it is 45%and 99%(nearly inactive)for PtCo TONPs and commercial Pt/C catalysts,respectively. | Qiqi Zhang Jialong Liu Tianyu Xia Jie Qi Haochang Lyu Baoyuan Luo Rongming Wang Yizhong Guo Lihua Wang and Shouguo Wang | 2019 | Nano Research2019，12，10： | 5 |
| 5 | Comprehensive strategy of conduit guidance combined with VEGF producing Schwann cells accelerates peripheral nerve repair显示文摘Peripheral nerve regeneration requires stepwise and well-organized establishment of microenvironment.Since local delivery of VEGF-A in peripheral nerve repair is expected to promote angiogenesis in the microenvironment and Schwann cells(SCs)play critical role in nerve repair,combination of VEGF and Schwann cells may lead to efficient peripheral nerve regeneration.VEGF-A overexpressing Schwann cells were established and loaded into the inner wall of hydroxyethyl cellulose/soy protein isolate/polyaniline sponge(HSPS)conduits.When HSPS is mechanically distorted,it still has high durability of strain strength,thus,can accommodate unexpected strain of nerve tissues in motion.A 10 mm nerve defect rat model was used to test the repair performance of the HSPS-SC(VEGF)conduits,meanwhile the HSPS,HSPS-SC,HSPS-VEGF conduits and autografts were worked as controls.The immunofluorescent co-staining of GFP/VEGF-A,Ki67 and MBP showed that the VEGF-A overexpressing Schwann cells could promote the proliferation,migration and differentiation of Schwann cells as the VEGF-A was secreted from the VEGF-A overexpressing Schwann cells.The nerve repair performance of the multifunctional and flexible conduits was examined though rat behavioristics,electrophysiology,nerve innervation to gastrocnemius muscle(GM),toluidine blue(TB)staining,transmission electron microscopy(TEM)and NF200/S100 double staining in the regenerated nerve.The results displayed that the effects on the repair of peripheral nerves in HSPS-SC(VEGF)group was the best among the conduits groups and closed to autografts.HSPS-SC(VEGF)group exhibited notably increased CD31+endothelial cells and activation of VEGFR2/ERK signaling pathway in the regenerated nerve tissues,which probably contributed to the improved nerve regeneration.Altogether,the comprehensive strategy including VEGF overexpressing Schwann cells-mediated and HSPS conduit-guided peripheral nerve repair provides a new avenue for nerve tissue engineering. | Ping Wu Zan Tong Lihua Luo Yanan Zhao Feixiang Chen Yinping Li Céline Huselstein Qifa Ye Qingsong Ye Yun Chen | 2021 | Bioactive Materials2021，6，10： | 4 |
| 6 | LiPO_(2)F_(2) electrolyte additive for high-performance Li-rich cathode material显示文摘Li-rich layered oxide cathodes have received considerable attention because of the high operating potential and specific capacity. However, the structural instability and parasitic reactions at high potential cause severe degradation of the electrochemical performance. In our studies, the cycling stability of Li_(1.14)Ni_(0.133)Co_(0.133)Mn_(0.544)O_(2) cathode is improved with LiPO_(2)F_(2) electrolyte additive. After 500 cycles, the capacity retention is increased from 53.6% to 85% at 3 C by LiPO_(2)F_(2) modification. This performance is mainly attributed to the enhanced interfacial stability of the Li-rich cathode. Based on systematic characterization, LiPO_(2)F_(2) additive was found to promote a stable interface film on the cathode surface during the cycling and mitigates the interfacial side reactions. This study provides new insights for improving high-potential Li-rich layered oxide batteries. | Bing Jiang Jingru Li Bi Luo Qizhang Yan Hao Li Lehao Liu Lihua Chu Yingfeng Li Qiaobao Zhang Meicheng Li | 2021 | Journal of Energy Chemistry2021，30，9： | 3 |
| 7 | Nonlinear relationship between enteric-coated mycophenolate sodium dose and mycophenolic acid exposure in Han kidney transplantation recipients显示文摘The aim of the research was to investigate the pharmacokinetics(PK) of enteric-coated mycophenolate sodium(EC-MPS) by quantification of the active metabolite of mycophenolic acid(MPA)after multiple escalating oral doses in Han kidney transplant recipients. A total of 28 Han postoperative kidney transplant recipients were given a multiple-dose of 540, 720 or 900 mg of EC-MPS two times a day in combination with tacrolimus for 6 days. Blood specimens were collected at each time point from0 to 12 h after EC-MPS administration. MPA plasma concentrations were measured by UPLC–UV. The relationship between the EC-MPS dose and its PK parameters was assessed. In the range from 540 to900 mg, C_(max) and AUC_(0–12h) did not increase with dose escalation. The AUC_(0–12h), C_(max), C_0 and T_(max) for the 540 720 and 900 mg doses were not significantly different, respectively(P 40.05). AUC_0–12 h and C_(max) were increased less than proportionally with increasing EC-MPS dose levels. Inter-individual variability in AUC_(0–12h), C_(max) and C_0 were considerable. Nonlinear PK relationships were found from the doses of 540–900 mg of EC-MPS. | Jun Zhang Mengmeng Jia Lihua Zuo Na Li Yonggang Luo Zhi Sun Xiaojian Zhang Zhenfeng Zhu | 2017 | Acta Pharmaceutica Sinica B2017，7，3： | 3 |
| 8 | The Popularization and Application of Cold Storage Red Blood Cells or Whole Blood at -80 ℃ of the Rh(D) Negative Patients in Surgical Operation显示文摘Summary: The efficiency of cold storage red blood cells (CSRBC) or whole blood at -80 ℃ used in 27 Rh(D) negative patients during surgical operation was reported. The Rh(D) negative patients received the transfusion of CSRBC or whole blood stored at -80 ℃ for 180 to 360 days. The changes in the indexes, such as blood TB, DB, K +, Na +, BUN, Cr, urine protein (URPO), UOB, Hb, HCT, serum total protein, relative to hemolytic reaction and blood volume before and after transfusion were observed. The results showed that after transfusion of CSRBC or whole blood 27 cases were negative for urine protein and UOB, and the levels of BUN and Cr were normal (P>0.05). Blood TB, DB, Hb, and HCT were increased, while pH, blood K + and blood Na + was normal with the difference being not significant before and after operation (P>0.05). Plasma protein was decreased, but there was no significant difference before and after operation (P>0.05). It was suggested that CSRBC or whole blood at -80 ℃ could be safely infused to the Rh(D) negative patients without side effects during the surgical operation. | YU Zhongqing(余忠清) HU Lihua(胡丽华) Han Min(韩敏) RAO Shenzong(饶神宗) LUO Chengwei(罗成伟) | 2002 | Journal of Huazhong University of Science and Technology(Medical Sciences)2002，22，2： | 3 |
| 9 | Effect of acupuncture plus conventional treatment on brain activity in ischemic stroke patients: a regional homogeneity analysis显示文摘OBJECTIVE: To determine differences in cerebral activity evoked by acupuncture and conventional stroke treatment, and identify the treatment targets.METHODS: In total, 21 patients were randomly divided into two groups. Group A(11 patients) received both acupuncture and conventional treatment, while group B(10 patients) received conventional treatment only. Resting-state functional magnetic resonance imaging(f MRI) was performed on each participant before and after treatment. Regional homogeneity analysis was performed to investigate the potential mechanism of acupuncture treatment by comparing differences in cerebral activity between treatments.RESULTS: Group A showed higher Re Ho in the frontal lobe(BA6, BA46), supra-marginal gyrus(BA40),middle temporal gyrus(BA21), cerebellum, and insula. Group B showed higher Re Ho in the frontal lobe(BA6) and parietal lobe(BA3, BA7).CONCLUSION: Acupuncture and conventional treatment triggered relatively different clinical efficacy and brain responses. Acupuncture treatment more significantly improved the symptoms of stroke patients. More marked changes in sensory,emotional, and motor areas(including the frontal lobe, middle temporal gyrus, cerebellum, and insula) might reflect the specific acupuncture mechanism. | Wu Ping Zeng Fang Li Yongxin Li Ji Qiu Lihua Qin Wei Luo Lu Yin Canxin Xiong Yan Bai Yu Wang Dan Zhou Yumei Liang Fanrong | 2017 | Journal of Traditional Chinese Medicine2017，37，5： | 3 |
| 10 | A cabazitaxel liposome for increased solubility, enhanced antitumor effect and reduced systemic toxicity显示文摘The potential side effects of cabazitaxel(CBZ)in the field of cancer treatment have become a great limitation to its further clinical application.Liposomal delivery is a well-established approach to increase the therapeutic index of hydrophobic drugs.In this study,a PEGmodified liposome was developed for efficiently encapsulating CBZ,thus enhancing its specific tumor inhibition effect and reducing the systemic toxicity.It was found that the loading efficiency of CBZ into the liposome could be improved with the increase of lipophilic materials,as it could be over 80%under the weight ratio of 20:1(total lipid:CBZ).The diameter of CBZ loaded liposome(CBZ@Lipo)was^100 nm.And the liposome suspending in aqueous medium was stable at 4°C for at least one month,according to the change of its size distribution.The killing ability of CBZ@Lipo to cancer cells was significantly lower comparing to that of CBZ solution,which could be attributed to the slow release of CBZ from the liposomes.However,CBZ@Lipo could induce an obvious apoptosis of the cancer cells at low concentration.Furthermore,CBZ@Lipo exhibited an expressively enhanced tumor growth inhibition effect comparing to CBZ solution.More importantly,CBZ@Lipo showed an obviously higher biosafety proved by lower hemolysis probability,stable body weight of mice during the whole experiment and no obvious lesion in histology analysis.Our work provided a useful reference of the formulation of CBZ,which had potential for greater clinical application. | Xiaoyi Yin Lihua Luo Wei Li Jie Yang Chunqi Zhu Mengshi Jiang Bing qin Xiaoling Yuan Hang Yin Yichao Lu Yongzhong Du Dawei Chen Jian You | 2019 | Asian Journal of Pharmaceutical Sciences2019，14，6： | 3 |
| 11 | Auxin response factor 6A regulates photosynthesis,sugar accumulation,and fruit development in tomato显示文摘Auxin response factors(ARFs)are involved in auxin-mediated transcriptional regulation in plants.In this study,we performed functional characterization of SlARF6A in tomato.SlARF6A is located in the nucleus and exhibits transcriptional activator activity.Overexpression of SlARF6A increased chlorophyll contents in the fruits and leaves of tomato plants,whereas downregulation of SlARF6A decreased chlorophyll contents compared with those of wild-type(WT)plants.Analysis of chloroplasts using transmission electron microscopy indicated increased sizes of chloroplasts in SlARF6A-overexpressing plants and decreased numbers of chloroplasts in SlARF6A-downregulated plants.Overexpression of SlARF6A increased the photosynthesis rate and accumulation of starch and soluble sugars,whereas knockdown of SlARF6A resulted in opposite phenotypes in tomato leaves and fruits.RNA-sequence analysis showed that regulation of SlARF6A expression altered the expression of genes involved in chlorophyll metabolism,photosynthesis and sugar metabolism.SlARF6A directly bound to the promoters of SlGLK1,CAB,and RbcS genes and positively regulated the expression of these genes.Overexpression of SlARF6A also inhibited fruit ripening and ethylene production,whereas downregulation of SlARF6A increased fruit ripening and ethylene production.SlARF6A directly bound to the SAMS1 promoter and negatively regulated SAMS1 expression.Taken together,these results expand our understanding of ARFs with regard to photosynthesis,sugar accumulation and fruit development and provide a potential target for genetic engineering to improve fruit nutrition in horticulture crops. | Yujin Yuan Xin Xu Zehao Gong Yuwei Tang Mengbo Wu Fang Yan Xiaolan Zhang Qian Zhang Fengqing Yang Xiaowei Hu Qichen Yang Yingqing Luo Lihua Mei Wenfa Zhang Cai-Zhong Jiang Wangjin Lu Zhengguo Li Wei Deng | 2019 | Horticulture Research2019，6，1： | 3 |
| 12 | Highly Selective Fluorescent Recognition towards Th4+ Based on Coumarin-derivatized Crescent Aromatic Oligoamide显示文摘intramolecularly 结合氢的新月状的芳香的 oligoamide 1 适用二香豆素残余被综合。从在金属离子 complexation 之上的紫外力、荧光灯的系列的结果证明用作形状坚持的脊梁的部分的二 fluorophores 在另外的 lanthanide 和 uranyl 离子上为 Th4+ 的识别向分子提供了高选择和敏感。A 48 褶层荧光灯在紧张的改进在增加 Th4+ 之上在 505 nm 被观察,当另外的金属阳离子没能导致如此的一个重要变化时。为 Th4+ 的视觉察觉被颜色变化完成。1 形成的建筑群和 Th4+ 的 stoichiometry 被发现是有稳定性常数的 1:1 (2. | Rongzhao Gao Jinchuan Hu Ke Zhang Youzhou He Peng Liu Shunzhong Luo Yanqiu Yang Liang Yang Wen Feng Lihua Yuan | 2013 | Chinese Journal of Chemistry2013，31，5： | 2 |
| 13 | Composition and origin of lipid biomarkers in the surface sediments from the southern Challenger Deep, Mariana Trench显示文摘The surface sediments collected from the southern Mariana Trench at water depths between ca.4900 m and 7068 m were studied using lipid biomarker analyses to reveal the origin and distribution of organic matters. For all samples, an unresolved complex mixture(UCM) was present in the hydrocarbon fractions, wherein resistant component tricyclic terpanes were detected but C_(27)-C _(29) regular steranes and hopanes indicative of a higher molecular weight range of petroleum were almost absent.This biomarker distribution patterns suggested that the UCM and tricyclic terpanes may be introduced by contamination of diesel fuels or shipping activities and oil seepage elsewhere. The well-developed faults and strike-slip faults in the Mariana subduction zone may serve as passages for the petroleum hydrocarbons. In addition, the relative high contents of even n-alkanes and low Carbon Preference Indices indicated that the n-alkanes were mainly derived from bacteria or algae. For GDGTs, the predominance of GDGT-0 and crenarchaeol, together with low GDGT-0/Crenarchaeol ratios(ranging from0.86 to 1.64), suggests that the GDGTs in samples from the southern Mariana Trench were mainly derived from planktic Thaumarchaeota. However, the high GDGT-0/crenarchaeol ratio(10.5) in sample BC07 suggests that the GDGTs probably were introduced by methanogens in a more anoxic environment. Furthermore, the n-alkanes C_(19)-C_(22) and the n-fatty acids C_(20:0)-C_(22:0) were depleted in^(13)C by 3‰ compared to n-alkanes C_(16)-C_(18) and the n-fatty acids C_(14:0)-C_(18:0), respectively, which was interpreted to result from the preferential reaction of fatty acid fragments with carbon 'lighter' terminal carboxyl groups during carbon chain elongation from the precursors to products. The abundance of total alkanes, carboxylic acids, alcohols and total lipids were generally increased along the down-going seaward plate, suggesting the lateral organic matter inputs play an important role in organic matter accumulation in hadal trenches. The extremely high contents of biomarkers in sample BC11 were most likely related to trench topography and current dynamics, since the lower steepness caused by graben texture and proximity to the trench axis may result in higher sedimentation rate. This paper, for the first time, showed the biomarker patterns in surface sediments of the Mariana Trench and shed light on biogeochemistry of the hardly reached trench environment. | Hongxiang Guan Linying Chen Min Luo Lihua Liu Shengyi Mao Huangmin Ge Mei Zhang Jiasong Fang Duofu Chen | 2019 | Geoscience Frontiers2019，10，1： | 2 |
| 14 | Protein adsorption on the poly(L-lactic acid) surface modified by chitosan and its derivatives显示文摘Surface modification of biomaterials has been adopted over the years to improve their biocompatibility. In this study, aiming to promote hydrophilicity and to introduce natural recognition sites onto poly(L-lactic acid) (PLLA) films, chitosan and its derivatives, carboxymethyl chitosan(CMC) and N-methylene phosphonic chitosan (NPC), were used to modify the surface of PLLA films by an entrapment method. Radiolabeled (125I) proteins were used to measure the amount of protein adsorbed to PLLA surfaces. Fibronectin (Fn) was used to study the protein adsorption on the modified PLLA surfaces, including isotherm adsorption and adsorption kinetics of single protein, competitive adsorption of binary proteins system and serum multi-proteins and the desorption behavior in serum solution. The results showed that in the isotherm adsorption, Fn had a larger adsorption capacity on the CS-modified surface at lower concentrations, but had a high adsorption capacity at CMC-modified surface at higher concentrations. In the study of absorption kinetics, Fn had a fastest adsorption equilibrium and a highest equilibrium adsorption capacity at the CS-modified surface, while it was opposite at the PCS-modified surface. When BSA and serum were added, it had the greatest effect on the adsorption of Fn on the PCS-modified surface. After 6 hours soaking in the desorption study, Fn had reached desorption equilibrium on all the modified surfaces, which had different effects on the desorption rate and the remaining percentage of Fn. | JIAO YanPeng ZHOU ChangRen LI LiHua DING Shan LU Lu LUO BingHong LI Hong | 2009 | Chinese Science Bulletin2009，54，18： | 2 |
| 15 | Phenotypic similarities in pigs with SOX10^(c.321dupC)and SOX10^(c.325A>T)mutations implied the correlation of SOX10 haploinsufficiency with Waardenburg syndrome显示文摘SOX10 is a causative gene of Waardenburg syndrome(WS)that is a rare genetic disorder characterized by hearing loss and pigment disturbance.More than 100 mutations of SOX10 have been found in patients with Type 2 WS(WS2),Type 4 WS(WS4),and more complex syndromes.However,no mutation hotspot has been detected in SOX10,and most cases are sporadic,making it difficult to establish a correlation between the high phenotypic and genetic variability.In this study,a duplication of the 321th cytosine(c.321dupC)was introduced into SOX10 in pigs,which induced premature termination of the translation of SOX10(p.K108QfsX45).The premature stop codon in Exon 3 triggered the degradation of mutant mRNA through nonsense-mediated mRNA decay.However,SOX10^(c.321dupC) induced a highly similar phenotype of WS2 with heterogeneous inner ear malformation compared with its adjacent missense mutation SOX10^(c.325A>T).In addition,a site-saturation mutation analysis of the SOX10 N-terminal nuclear localization signal(n-NLS),where these two mutations located,revealed the correlation between SOX10 haploinsufficiency and WS by an in vitro reporter assay.The analysis combining the in vitro assay with clinical cases may provide a clue to clinical diagnoses. | Tingting Lin Lihua Luo Weiwei Guo Wei Ren Chuanhong Liu Hong Wei Shiming Yang Yong Wang | 2020 | Journal of Genetics and Genomics2020，47，12： | 2 |
| 16 | Neuroprotective effect of the traditional Chinese herbal formula Tongxinluo: a PET imaging study in rats显示文摘Tongxinluo has been widely used in China for the treatment of acute stroke and for neuroprotection. However, there are few positron emission tomography(PET) studies on the neuroprotective effect of Tongxinluo on cerebral ischemia/reperfusion in small animals. In the present study, Tongxinluo superfine powder suspension or its vehicle was administered intragastrically to rats for 5 successive days before middle cerebral artery occlusion. 18F-fluorodeoxyglucose(FDG) small animal PET imaging showed that at 1 and 2 weeks after cerebral ischemia/reperfusion, glucose metabolism in the ischemic area was greater in rats that had received Tongxinluo than in those that had received the vehicle. Nissl staining showed that 2 weeks after cerebral ischemia/reperfusion, there was less neuronal loss in the prefrontal cortex in Tongxinluo-treated rats than in controls. In addition, Tongxinluo-treated animals showed better neurologic function and lower cerebral infarct volume than rats that received the vehicle. These findings suggest that Tongxinluo exhibits neuroprotective effects in cerebral ischemia/reperfusion injury and demonstrates that 18F-FDG small animal PET imaging is a useful tool with which to study the molecular pharmacology of traditional Chinese medicine. | Xiao Cheng Haoxuan Luo Lihua Zhou Lixin Wang Jingbo Sun Yan Huang Enli Luo Yefeng Cai | 2014 | Neural Regeneration Research2014，9，13： | 2 |
| 17 | Nitrogen content diagnosis of apple trees canopies using hyperspectral reflectance combined with PLS variable extraction and extreme learning machine显示文摘Nitrogen(N)is an important mineral element in apple production.Rapid estimation of apple tree N status is helpful for achieving precise N management.The objective of this work was to explore partial least squares(PLS)regression in dimensional reduction of spectral data and build the diagnostic model.The spectral reflectance data were collected from Fuji apple trees with 4 levels of N fertilizer treatment in the Loess Plateau in 2018 and 2019 using an ASD portable spectroradiometer,and leaf total N content was obtained at the same time.The raw spectra were pretreated using Savitzky-Golay(SG)smoothing and a combination of SG and first-order derivative(SG_FD)or second-order derivative(SG_SD).The samples were divided into a calibration dataset and a prediction dataset using SPXY.Based on 4 factors of PLS regression,including latent variables(LVs),X-loading,variable importance in projection(VIP)and regression coefficients(RC),the 6 methods(LVs,X-loading,VIP_01,VIP_02,RC_01 and RC_02)were derived and used for variable extraction,based on which PLS model and ELM model were established.The results indicated that the spectral data processed by SG_FD had the highest signal-to-noise ratio and was selected for subsequent analysis.The amounts of variables extracted by LVs,X-loading,VIP_01,VIP_02,RC_01 and RC_02 were 6,11,18,305,26 and 88,respectively.The method of extracting variables with an RC threshold based on the minimum RMSEP(RC_02)could effectively avoid the omission of effective information.The RC_02 method was recommended for related research which required accurate wavelength information as a variable.The variable extraction method based on LVs generated an ELM model with a simple structure.The prediction results showed that the ELM model outperformed the PLS model.The PLS(LVs)_ELM model was the best;R2P,RMSEP and RPD were 0.837,2.393 and 2.220,respectively. | Shaomin Chen Lihui Ma Tiantian Hu Lihua Luo Qiong He Shaowu Zhang | 2021 | International Journal of Agricultural and Biological Engineering2021，14，3： | 2 |
| 18 | In situ atomic-scale observation of dislocation behaviors in twin-structured Pt nanocrystals显示文摘The deformation mechanisms of twin-structured metallic materials have attracted great interest.Though previous theoretical predictions have suggested that the repulsive force of the twin boundary(TB)can significantly affect the deformation of twinstructured metals,it remains unclear whether this prediction applies to experimental conditions.In this paper,the atomic-scaled deformation process of twin-structured Pt nanocrystals was in situ observed using our home-made device in a high-resolution transmission electron microscope.We have shown that the plastic deformation of the twin-structured Pt nanocrystals was governed by full dislocation generation as well as Lomer dislocation(LD)lock formation and destruction.After LD locks were destructed,these full dislocations tended to move towards the surface of the nanocrystals.The findings revealed that due to the ultra-high repulsive force of TB on dislocation,there was no dislocation-TB reaction during the deformation.These findings can enrich our understanding of the dislocation behaviors of twin-structured nanocrystals. | GUO YiZhong SUN Tao FU LiBo HAO LongHu HUANG Ming WEI RuJian LUO JunFeng HE Xin WANG XingQuan XIONG XiaoDong WANG LiHua HAN XiaoDong | 2021 | Science China(Technological Sciences)2021，64，3： | 2 |
| 19 | H5N6禽流感病毒的基因起源、进化和流行显示文摘2014~2016年间对我国16个省份活禽市场的监测,共鉴定了3174份流感阳性样品,分离并测序了1135份禽流感病毒,分属于31种亚型。 | yuhai bi quanjiao chen qianli wang jianjun chen tao jin gary wong chuansong quan jun liu jun wu renfu yin lihua zhao mingxin li zhuang ding rongrong zou wen xu hong li huijun wang kegong tian guanghua fu yu huang alexander shestopalov shoujun li bing xu hongjie yu tingrong luo lin lu xun xu yang luo 刘映霞 史卫峰 刘翟 高福 | 2017 | 科学新闻2017，0，4： | 2 |
| 20 | Actin-binding Rho activating protein is expressed in the central nervous system of normal adult rats显示文摘Previous studies show that actin-binding Rho activating protein (Abra) is expressed in cardiomyocytes and vascular smooth muscle cells.In this study,we investigated the expression profile of Abra in the central nervous system of normal adult rats by confocal immunofluorescence.Results showed that Abra immunostaining was located in neuronal nuclei,cytoplasm and processes in the central nervous system,with the strongest staining in the nuclei;in the cerebral cortex,Abra positive neuronal bodies and processes were distributed in six cortical layers including molecular layer,external granular layer,external pyramidal layer,internal granular layer,internal pyramidal layer and polymorphic layer;in the hippocampus,the cell bodies of Abra positive neurons were distributed evenly in pyramidal layer and granular layer,with positive processes in molecular layer and orien layer;in the cerebellar cortex,Abra staining showed the positive neuronal cell bodies in Purkinje cell layer and granular layer and positive processes in molecular layer;in the spinal cord,Abra-immunopositive products covered the whole gray matter and white matter;co-localization studies showed that Abra was co-stained with F-actin in neuronal cytoplasm and processes,but weakly in the nuclei.In addition,in the hippocampus,Abra was co-stained with F-actin only in neuronal processes,but not in the cell body.This study for the first time presents a comprehensive overview of Abra expression in the central nervous system,providing insights for further investigating the role of Abra in the mature central nervous system. | Lihua Liu Mingying Luo Baolin Yang Xiaoqiong Wu Wu Zhu Yinglu Guan Weijun Cai Kerstin Troidl Wolfgang Schaper Jutta Schaper | 2012 | Neural Regeneration Research2012，7，13： | 2 |
您所在的机构暂未开通此部分的功能，请联系管理员