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| 1 | Channel List Selection Based on Quality Prediction in WirelessHART Networks显示文摘WirelessHART is one of the most widely used technologies in industrial wireless networks.However,its performance is highly influenced by the quality of wireless channels.To improve the reliability of wireless communications,WirelessHART employs channel blacklisting and channel hopping mechanisms,which highlights the importance of channel assessment.Traditional methods generally resort to packet reception ratio(PRR)of the previous time slot to assess and allocate channels,but this is not accurate.In this paper,we propose a learning-based framework for predicting the PRR,and on the basis of the predicted PRR,we develop a heuristic channel selection algorithm to confirm the channel list,which takes into account the balance of channel diversity and route diversity.Simulation results demonstrate that our algorithm outperforms existing ones in terms of achieved reliability. | Gongpu Chen Rui Ma Mengdan Lei Xianghui Cao | 2018 | Journal of Communications and Information Networks2018，3，3： | 1 |
| 2 | cFLIPS regulates alternative NLRP3 inflammasome activation in human monocytes显示文摘The innate immune responses,including inflammasome activation,are paramount for host defense against pathogen infection.In contrast to canonical and noncanonical inflammasome activation,in this study,heat-killed gram-negative bacteria(HK bacteria)were identified as single-step stimulators of the NLRP3 inflammasome in human monocytes,and they caused a moderate amount of IL-1βto be released from cells.Time course experiments showed that this alternative inflammasome response was finished within a few hours.Further analysis showed that the intrinsically limited NLRP3 inflammasome activation response was due to the negative regulation of caspase-8 by the short isoform of cFLIP(cFLIPs),which was activated by NF-κB.In contrast,overexpressed cFLIPS,but not overexpressed cFLIPL,inhibited the activation of caspase-8 and the release of IL-1βin response to HK bacteria infection in human monocytes.Furthermore,we demonstrated that TAK1 activity mediated the expression of cFLIPs and was upstream and essential for the caspase-8 cleavage induced by HK bacteria in human monocytes.The functional specificity of cFLIPs and TAK1 revealed unique responses of human monocytes to a noninvasive pathogen,providing novel insights into an alternative regulatory pathway of NLRP3 inflammasome activation. | Yuhui Gao Shi Yu Mengdan Chen Xun Wang Lei Pan Bin Wei Guangxun Meng | 2023 | Cellular & Molecular Immunology2023，20，10： | 0 |
| 3 | Propofol terminates ventricular fibrillation storm caused by pulmonary embolism显示文摘To the editor:In March 2009,a 56-year-old woman was referred to our hospital for chest discomfort that had lasted for 12 hours.ECG revealed frequent ventricular premature beats and a complete right bundle branch block.Serum CK and cardiac troponin I was normal.Serum biochemistry and blood gas analysis were unremarkable. | Hong Jiang Xu Mengdan Kong Ailing Liu Qiang Chen Rong Dai Qiuyan Wang Lexin Sun Baogui | 2014 | Chinese Medical Journal2014，，21： | 0 |
| 4 | Identification of neutral genome integration sites with high expression and high integration efficiency in Fusarium venenatum TB01显示文摘CRISPR/Cas9-mediated homology-directed recombination is an efficient method to express target genes.Based on the above method,providing ideal neutral integration sites can ensure the reliable,stable,and high expression of target genes.In this study,we obtained a fluorescent transformant with neutral integration and high expression of the GFP expression cassette from the constructed GFP expression library and named strain FS.The integration site mapped at 4886 bp upstream of the gene FVRRES_00686 was identified in strain FS based on a Y-shaped adaptor-dependent extension,and the sequence containing 600 bp upstream and downstream of this site was selected as the candidate region for designing sgRNAs(Sites)for CRISPR/Cas9-mediated homology-directed recombination.PCR analysis showed that the integration efficiency of CRISPR/Cas9-mediated integration of target genes in designed sites reached 100%.Further expression stability and applicability analysis revealed that the integration of the target gene into the above designed sites can be stably inherited and expressed and has no negative effect on the growth of F.venenatum TB01.These results indicate the above designed neutral sites have the potential to accelerate the development of F.venenatum TB01 through overexpression of target genes in metabolic engineering. | Sheng Tong Kexin An Wuxi Chen Mengdan Chai Yuanxia Sun Qinhong Wang Demao Li | 2023 | Synthetic and Systems Biotechnology2023，8，1： | 0 |
| 5 | Conductive hydrogels incorporating carbon nanoparticles:A review of synthesis,performance and applications显示文摘As one of the most rapidly expanding materials,hydrogels have gained increasing attention in a variety of fields due to their biocompatibility,degradability and hydrophilic properties,as well as their remarkable adhesion and stretchability to adapt to different surfaces.Hydrogels combined with carbon-based materials possess enhanced properties and new functionalities,in particular,conductive hydrogels have become a new area of research in the field of materials science.This review aims to provide a comprehensive overview and up-to-date examination of recent developments in the synthesis,properties and applications of conductive hydrogels incorporating several typical carbon nanoparticles such as carbon nanotubes,graphene,carbon dots and carbon nanofibers.We summarize key techniques and mechanisms for synthesizing various composite hydrogels with exceptional properties,and represented applications such as wearable sensors,temperature sensors,supercapacitors and human-computer interaction reported recently.The mechanical,electrical and sensing properties of carbon nanoparticles conductive hydrogels are thoroughly analyzed to disclose the role of carbon nanoparticles in these hydrogels and key factors in the microstructure.Finally,future development of conductive hydrogels based on carbon nanoparticles is discussed including the challenges and possible solutions in terms of microstructure optimization,mechanical and other properties,and promising applications in wearable electronics and multifunctional materials. | Shipeng Zhang Bo Zhao Ding Zhang Mengdan Yang Xinguang Huang Lei Han Kun Chen Xinjian Li Rui Pang Yuanyuan Shang Anyuan Cao | 2023 | Particuology2023，，12： | 0 |
| 6 | Chemokine Ligand 13 Expression is Abundant in the Tumor Microenvironment and Indicates Poor Prognosis of Kidney Clear Cell Carcinoma显示文摘The chemokine ligand 13-chemokine receptor 5(CXCL13-CXCR5)axis has been characterized as a critical tumor-promoting signaling pathway in the tumor microenvironment(TME)in multiple types of solid tumors.In this study,we analyzed the expression profile of CXCL13 in kidney clear cell carcinoma(KIRC)and its correlation with tumor-infiltrating immune cells(TIICs).A monoclonal antibody against CXCL13 with high affinity and purity was generated in our lab for western blot and immunohistochemistry(IHC).Bioinformatic analysis was performed based on bulk-seq data from the Cancer Genome Atlas(TCGA)-KIRC and single-cell RNA-seq data from scRNASeqDB and PanglaoDB.Results showed that high CXCL13 expression in TME was associated with shorter progression-free survival(PFS),disease-specific survival(DSS),and overall survival(OS).KIRC cell lines,as well as several other cancer cell lines,had negative CXCL13 expression.IHC staining from the Human Protein Atlas(HPA)and our tissue array indicated that CXCL13 might be mainly expressed by TIICs,but not KIRC tumor cells.CXCL13 expression was strongly and positively correlated withγδT cell abundance in TME.Besides,γδT cell infiltration was associated with poor survival of KIRC.Methylation 450k array data showed that CXCL13 promoter hypomethylation was common in TIICs.The methylation level of cg16361705 within the CXCL13 promoter might play an important role in modulating CXCL13 transcription.In conclusion,our study revealed that CXCL13 expression andγδT cell infiltration in TME is associated with unfavorable survival of KIRC.TIICs,most possiblyγδT cells,are the dominant source of CXCL13 in KIRC TME. | MENGDAN WU MENGYAO SUN QINHUAI LAI YIN LU YUYIN FU YUJIA PENG WEIRONG LAI LISHI ZENG SHENGYAN ZHAO YUYAN LI ZHIXIONG ZHANG XIAOFENG CHEN FAN QIAO YIWEN ZHANG SHIJIE ZHOU LANTU GOU JINLIANG YANG | 2021 | BIOCELL2021，45，3： | 0 |
| 7 | TAK1 Deficiency in Macrophages Increases Host Susceptibility to Leishmania Infection显示文摘Leishmania parasites mainly infect macrophages and may cause severe immunopathologies in their host,which are called leishman-iases.In the current work,we infected human and mouse macrophages in vitro with Leishmania major,an etiological agent of cu-taneous leishmaniasis,and found that inhibition or deletion of the transforming growth factorβ–activated kinase 1(TAK1)gene re-sulted in increased parasite loads.In vivo,following a challenge with L.major,mice with a macrophage-specific deletion of TAK1 showed increased clinical signs and higher parasite loads compared with wild-type controls.TAK1 deficiency in mouse macro-phages led to biased Th2 cell responses during the acute stage of infection,characterized by a decrease in interferon-γexpression,and increased expression of IL-4,IL-5 and IL-10.Finally,we found that,in the late stage of L.major infection,excessive Th2-related cytokines led to high arginase 1 expression in mouse tissues and a significant reduction of NO production both locally and system-ically,resulting in compromised control of Leishmania.These findings suggest that TAK1 plays a vital role in host resistance to Leish-mania infection. | Xiankai Cao Kossiwa C.Kokou Shi Yu Mengdan Chen Junling Niu HervéLecoeur Eric Prina Gerald F.Späth Guangxun Meng | 2023 | Infectious Microbes & Diseases2023，5，3： | 0 |
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