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| 1 | Interferon-α response in chronic hepatitis B-transfected HepG2.2.15 cells is partially restored by lamivudine treatment显示文摘AIM: To characterize the IFN-response and its modul- ation by the antiviral compound lamivudine in HBV- transfected HepG2.2.15 cells. METHODS: HepG2.2.15 and HepG2 cells were stimulated with various concentrations of IFN-a2a in the presence or absence of lamivudine. Then, total RNA was extracted and analysed by customised cDNA arrays and northern blot for interferon-inducible genes (ISGs). In addition, cellular proteins were extracted for EMSA and western blot. HBV replication was assessed by southern blot or ELISAs for HBsAg and HBeAg. RESULTS: Two genes (MxA, Cig5) with completely abolished and 4 genes (IFITM1, -2, -3, and 6-16) with partially reduced IFN-responses were identified in HepG2.2.15 cells. In 2 genes (IFITM1, 6-16), the response to IFN-a could be restored by treatment with lamivudine. This effect could not be explained by a direct modulation of the Jak/Stat signalling pathway since EMSA and western blot experiments revealed no suppression of Stat1 activation and ISGF3 formation after stimulation with IFN-a in HepG2.2.15 compared to HepG2 cells. CONCLUSION: These results are consistent with the assumption that chronic hepatitis B may specifically modulate the cellular response to IFN by a selective blockage of some ISGs. Antiviral treatment with lamivudine may partially restore ISG expression by reducing HBV gene expression and replication. | Shi-He Guan Mengji Lu Petra Grünewald Michael Roggendorf Guido Gerken Joerg F Schlaak | 2007 | World Journal of Gastroenterology2007，13，2： | 22 |
| 2 | Contribution of Toll-like receptors to the control of hepatitis B virus infection by initiating antiviral innate responses and promoting specific adaptive immune responses显示文摘适应免疫在肝炎 B 的控制起一个关键作用,这很好被接受病毒(HBV ) 感染。相反,天生的免疫的贡献仅仅在最近的年里收到了注意。像使用费的受体(TLR ) 察觉到联系病原体的分子模式并且激活抗病毒的机制,包括细胞内部的抗病毒的小径和抗病毒的受动器干扰素(IFN ) 和支持 inflammatory cytokines 的生产。从在 vitro 并且在 vivo 模型的试验性的结果证明了 TLR 调停细胞的发信号的小径和抗病毒的 cytokines 的生产的激活,导致 HBV 复制的抑制。然而, HBV 感染在主人房间和下游地表明小径的激活的封锁上与 TLR 表示的 downregulation 被联系。在主要 HBV 感染, TLR 可以减慢 HBV 感染,但是仅仅间接地作出贡献到病毒的清理。重要地, TLR 可以在 vivo 调制 HBV 特定的 T 房间和 B 房间回答,它为 HBV 感染的结束是必要的。因此, TLR 收缩筋正在答应候选人为长期的 HBV 感染的处理充当 immunomodulators。抗病毒的处理可以在长期的 HBV 感染恢复 TLR 表示和功能并且可以基于 TLR 激活增加治疗学的途径的功效。有抗病毒的治疗和 TLR 激活的联合治疗学的策略能便于 HBV 特定的有免疫力的回答的恢复并且从而,在长期地感染的 HBV 病人完成病毒的清理。 | Zhiyong Ma Ejuan Zhang Dongliang Yang Mengji Lu | 2015 | Cellular & Molecular Immunology2015，12，3： | 22 |
| 3 | Epidemiological Characteristics of Newly-Reported HIV Cases Among Youth Aged 15−24 Years-China, 2010−2019显示文摘Summary What is already known about this topic?An estimated 1,400 new cases of human immunodeficiency virus(HIV)occur every day among youths globally.However,HIV distribution among youth aged 15–24 years in China has not been researched extensively. | Hao Zhao Hui Liu Lu Wang Xuan Yang Shaorong Wang Mengjie Han Jian Li | 2020 | China CDC weekly2020，2，48： | 23 |
| 4 | Construction and Identification of a Human Liver Specific MicroRNA Eukaryotic Expression Vector显示文摘MiR-122 is one of the non-coding RNAs which showed its effects on the lipo-metablism,virus infection and HCC forming through regulation of liver gene expression.Its eukaryotic expression vector was constructed by using pSuper which was widely applied in the siRNA expression.The precursor of human miR-122 gene was amplified by polymerase chain reaction(PCR)from the human genomic DNA.The positive clones were screened by PCR and restriction enzyme digestion.The new expression vector of miR-122 was named pHsa-m122.PHsa-m122 and its controls were transfected to HepG2 cells.The miR-122 expression activity was evaluated by GFP122i sensor reporter plasmid through fluorescence detection and Western blot.It was shown that the fluorescence intensity of GFP122si and pHsa-m122 co-transfection group was weaker than that of the controls,so the functional activity of expressed miR-122 was detected.When HepG2 cells were co-transfected with HBV1.3 and pHsa-m122 plasmids, the results showed miR-122 may down-regulate the gene expression of HBV.The human liver specific microRNA eukaryotic expression vector of miR-122 was constructed successfully,which may facilitate further study of its function in the development of liver virus infection diseases and HCC. | Shanshan Chen Ming Ni Bing Yu Tingting Lv Mengji Lu Feili Gong | 2007 | Cellular & Molecular Immunology2007，4，6： | 18 |
| 5 | Recent advances in energy storage mechanism of aqueous zinc-ion batteries显示文摘Aqueous rechargeable zinc-ion batteries(ZIBs)have recently attracted increasing research interest due to their unparalleled safety,fantastic cost competitiveness and promising capacity advantages compared with the commercial lithium ion batteries.However,the disputed energy storage mechanism has been a confusing issue restraining the development of ZIBs.Although a lot of efforts have been dedicated to the exploration in battery chemistry,a comprehensive review that focuses on summarizing the energy storage mechanisms of ZIBs is needed.Herein,the energy storage mechanisms of aqueous rechargeable ZIBs are systematically reviewed in detail and summarized as four types,which are traditional Zn^(2+)insertion chemistry,dual ions co-insertion,chemical conversion reaction and coordination reaction of Zn^(2+)with organic cathodes.Furthermore,the promising exploration directions and rational prospects are also proposed in this review. | Duo Chen Mengjie Lu Dong Cai Hang Yang Wei Han | 2021 | Journal of Energy Chemistry2021，30，3： | 12 |
| 6 | New therapeutic vaccination strategies for the treatment of chronic hepatitis B显示文摘Chronic hepatitis B virus(CHB) is currently treated with either interferon-based or nucleot(s)idebased antiviral therapies.However,treatment with pegylated interferon alpha results in a durable antiviral response in only about 30%patients and is associated with side effects.Most patients receiving nucleot(s)ide analogue treatment do not establish long-term,durable control of Infection and have rebounding viremia after cessation of therapy.Thus,novel therapy strategies are necessary to achieve the induction of potent and durable antiviral immune responses of the patients which can maintain long-term control of viral replication.Therapeutic vaccination of HBV carriers is a promising strategy for the control of hepatitis B.Here the authors review new therapeutic vaccination strategies to treat chronic hepatitis B which may be introduced for patient treatment in the future. | Jia Liu Anna Kosinska Mengji Lu Michael Roggendorf | 2014 | Virologica Sinica2014，29，1： | 9 |
| 7 | The role of micro RNAs in hepatocyte metabolism and hepatitis B virus replication显示文摘Though efficient vaccines against hepatitis B virus(HBV) and antiviral therapies are available,chronic HBV infection is still a global health problem. The process of HBV infection and HBV life cycle are extensively studied in last decades, however, the mechanisms of HBV-induced alterations of host cell metabolisms and host factors involved in modulating of viral replication are not fully understood. Thus, it is an important issue to examine these specific HBV-host interactions for development of novel strategies for antiviral therapies. Recently, microRNAs(miRNAs), a class of post-transcriptional regulatory small RNA, seem to be the relevant fine tuning factors of various cellular activities and pathways, including cell growth, metabolism, and viral replication. In this review, we summarize the up to date knowledge concerning the virus-host interactions and emphasizing on the role of miRNAs in regulation of HBV replication and host cell metabolism. | Wanyu Deng Mengji Lu | 2016 | Virologica Sinica2016，31，6： | 7 |
| 8 | A Case of Hepatitis B Reactivation due to the Hepatitis B Virus Escape Mutant in a Patient undergoing Chemotherapy显示文摘A 62-year-old man had chronic hepatitis B virus (HBV) infection and was diagnosed with liver cirrhosis.At the time of diagnosis the patient's virologic markers were positive for hepatitis B surface antigen (HBsAg),antibody to hepatitis B e antigen (anti-HBe) and antibody to hepatitis B core antigen (anti-HBc),while antibody to hepatitis B surface antigen (anti-HBs) and HBV DNA were negative.Later the patient received chemotherapy for malignancy.However,this was interrupted due to elevated liver enzymes.At the same time HBV DNA became positive.Lamivudine (LMV) therapy was administered immediately.However,the levels of serum aminotransferase and total bilirubin (TB) were still rising.Finally the patient died of fulminant hepatic failure.A sequence revealed HBV genotype C (HBsAg subtype adw) with immune escape mutations,F8L,S34L,F41S,G44V,F93C,V96G,L110I,C149Y and F161Y.The high morbidity and mortality of this complication is one of the major obstacles to completing the standard treatment for malignancy in HBV carriers.Therefore,the relative risk of antiviral prophylactic failure should be further assessed and the optimal strategy for antiviral prophylaxis in HBsAg-positive patients with oncologic and hematologic malignancies undergoing chemotherapy should be revised. | Chunchen Wu Hui Shi Yun Wang Mengji LU Yang Xu Xinwen Chen | 2012 | Virologica Sinica2012，27，6： | 7 |
| 9 | The IL-1R/TLR signaling pathway is essential for efficient CD8+ T-cell responses against hepatitis B virus in the hydrodynamic injection mouse model显示文摘The outcome of hepatitis B viral(HBV)infection is determined by the complex interactions between replicating HBV and the immune system.While the role of the adaptive immune system in the resolution of HBV infection has been studied extensively,the contribution of innate immune mechanisms remains to be defined.Here we examined the role of the interleukin-1 receptor/Toll-like receptor(IL-1R/TLR)signaling pathway in adaptive immune responses and viral clearance by exploring the HBV mouse model.Hydrodynamic injection with a replication-competent HBV genome was performed in wild-type mice(WT)and a panel of mouse strains lacking specific innate immunity component expression.We found higher levels of HBV protein production and replication in Tlr2^(−/−),Tlr23479^(−/−),3d/Tlr24^(−/−),Myd88/Trif^(−/−)and Irak4^(−/−)mice,which was associated with reduced HBV-specific CD8+T-cell responses in these mice.Importantly,HBV clearance was delayed for more than 2 weeks in 3d/Tlr24^(−/−),Myd88/Trif^(−/−)and Irak4^(−/−)mice compared to WT mice.HBV-specific CD8+T-cell responses were functionally impaired for producing the cytokines IFN-γ,TNF-αand IL-2 in TLR signaling-deficient mice compared to WT mice.In conclusion,the IL-1R/TLR signaling pathway might contribute to controlling HBV infection by augmenting HBV-specific CD8+T-cell responses. | Zhiyong Ma Jia Liu Weimin Wu Ejuan Zhang Xiaoyong Zhang Qian Li Gennadiy Zelinskyy Jan Buer Ulf Dittmer Carsten J Kirschning Mengji Lu | 2017 | Cellular & Molecular Immunology2017，14，12： | 6 |
| 10 | Hepatitis B virus is degraded by autophagosome-lysosome fusion mediated by Rab7 and related components显示文摘Dear Editor,With an estimated 240 million chronically infected people worldwide,hepatitis B virus(HBV)infection is a major public health problem(Schweitzer et al.,2015).Despite more than 30 years of in tense research,many aspects of the HBV life cycle still remain unknown. | Yong Lin Chunchen Wu Xueyu Wang Thekla Kemper Anthony Squire Matthias Gunzer Jiming Zhang Xinwen Chen Mengji Lu | 2019 | Protein & Cell2019，10，1： | 5 |
| 11 | Patients with SARS-CoV-2 and HBV coinfection are at risk of greater liver injury显示文摘To date,it remains unclear if severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)co-infection exacerbates liver injury in patients with chronic hepatitis B virus(HBV)infection.In this study,we present a retrospective study of 133 hospitalized confirmed mild coronavirus disease 2019(COVID-19)cases,including 116 patients with COVID-19 with negative serum hepatitis B antigen and 17 HBV inactive carriers with COVID-19.We found that there were no significant differences for the discharge rate or duration of hospitalization be-tween the two groups.However,inactive HBV carriers with SARS-CoV-2 co-infection are at a higher risk of abnormal liver function tests.The enhanced liver injury induced by SARS-CoV-2 and HBV co-infection was identified as the hepatocyte type rather than the cholangiocyte type.Moreover,the inflammatory response,including abnormal lactate dehydrogenase,D-dimer and interleukin-6 production,may contribute to this injury following SARS-CoV-2 co-infection.Collectively,SARS-CoV-2 and HBV co-infection exacerbates liver function of the pa-tients with COVID-19. | Yong Lin Jun Yuan Quanxin Long Jieli Hu Haijun Deng Zhenyu Zhao Juan Chen Mengji Lu Ailong Huang | 2021 | Genes & Diseases2021，8，4： | 5 |
| 12 | Discrimination of False Negative Results in RT-PCR Detection of SARS-CoV-2 RNAs in Clinical Specimens by Using an Internal Reference显示文摘Reverse transcription-polymerase chain reaction(RT-PCR)is an essential method for specific diagnosis of SARS-CoV-2 infection.Unfortunately,false negative test results are often reported.In this study,we attempted to determine the principal causes leading to false negative results of RT-PCR detection of SARS-CoV-2 RNAs in respiratory tract specimens.Multiple sputum and throat swab specimens from 161 confirmed COVID-19 patients were tested with a commercialfluorescent RT-PCR kit targeting the ORF1 ab and N regions of SARS-CoV-2 genome.The RNA level of a cellular housekeeping gene ribonuclease P/MRP subunit p30(RPP30)in these specimens was also assessed by RT-PCR.Data for a total of 1052 samples were retrospectively re-analyzed and a strong association between positive results in SARS-CoV-2 RNA tests and high level of RPP30 RNA in respiratory tract specimens was revealed.By using the ROC-AUC analysis,we identified Ct cutoff values for RPP30 RT-PCR which predicted false negative results for SARS-CoV-2 RT-PCR with high sensitivity(95.03%–95.26%)and specificity(83.72%–98.55%)for respective combination of specimen type and ampli-fication reaction.Using these Ct cutoff values,false negative results could be reliably identified.Therefore,the presence of cellular materials,likely infected host cells,are essential for correct SARS-CoV-2 RNA detection by RT-PCR in patient specimens.RPP30 could serve as an indicator for cellular content,or a surrogate indicator for specimen quality.In addition,our results demonstrated that false negativity accounted for a vast majority of contradicting results in SARS-CoV-2 RNA test by RT-PCR. | Yafei Zhang Changtai Wang Mingfeng Han Jun Ye Yong Gao Zhongping Liu Tengfei He Tuantuan Li Mengyuan Xu Luping Zhou Guizhou Zou Mengji Lu Zhenhua Zhang | 2020 | Virologica Sinica2020，35，6： | 5 |
| 13 | Inducible Rubicon facilitates viral replication by antagonizing interferon production显示文摘The RUN domain Beclin-1-interacting cysteine-rich-containing(Rubicon)protein is involved in the maturation step of autophagy and the endocytic pathway as a Beclin-1-binding partner,but little is known regarding the role of Rubicon during viral infection.Here,we performed functional studies of the identified target in interferon(IFN)signaling pathways associated with Rubicon to elucidate the mechanisms of viral resistance to IFN.The Rubicon protein levels were elevated in peripheral blood mononuclear cells,sera and liver tissues from patients with hepatitis B virus(HBV)infection relative to those in healthy individuals.Assays of the overexpression and knockdown of Rubicon showed that Rubicon significantly promoted HBV replication.In addition,Rubicon knockdown resulted in the inhibition of enterovirus 71,influenza A virus and vesicular stomatitis virus.The expression o0f Rubicon led to the suppression of virus-induced type-I interferon(IFN-αand IFN-β)and type-III interferon(IFN-λ1).Translocation of activated IRF3 and IRF7 from the cytoplasm to the nucleus was involved in this process,and the NF-κB essential modulator(NEMO),a key factor in the IFN pathway,was the target with which Rubicon interacted.Our results reveal a previously unrecognized function of Rubicon as a virus-induced protein that binds to NEMO,leading to the inhibition of type-I interferon production.Rubicon thus functions as an important negative regulator of the innate immune response,enhances viral replication and may play a role in viral immune evasion. | Yushun Wan Wei Cao Tao Han Sheng Ren Jian Feng TieLong Chen Jun Wang Ruth Broering Mengji Lu Ying Zhu | 2017 | Cellular & Molecular Immunology2017，14，7： | 4 |
| 14 | Complementation of Wild-Type and Drug-Resistant Hepatitis B Virus Genomes to Maintain Viral Replication and Rescue Virion Production under Nucleos(t)ide Analogs显示文摘As the open reading frames of hepatitis B virus(HBV)genomes are overlapping,resistance mutations(MTs)in HBV polymerase may result in stop codon MTs in hepatitis B surface proteins,which are usually detected as a mixed population with wild-type(WT)HBV.The question was raised how the coexistence of nucleos(t)ide analogs(NAs)resistance MTs and WT sequences affects HBV replication.In the present study,HBV genomes with frequently detected reverse transcriptase(RT)/surface truncation MTs,rtA181T/sW172^*,rtV191I/sW182^*and rtM204I/sW196^*,were phenotypically characterized alone or together with their WT counterparts in different ratios by transient transfection in the absence or presence of Nas.In the absence of Nas,RT/surface truncation MTs impaired the expression and secretion of HBV surface proteins,and had a dose-dependent negative effect on WT HBV virion secretion.However,in the presence of Nas,coexistence of MTs with WT maintained viral replication,and the presence of WT was able to rescue the production of MT HBV virions.Our findings reveal that complementation of WT and MT HBV genomes is highly effective under drug treatment. | Chunchen Wu Baolin Li Xiaoyong Zhang Kaitao Zhao Yingshan Chen Yifei Yuan Yan Liu Rongjuan Chen Dongping Xu Xinwen Chen Mengji Lu | 2019 | Virologica Sinica2019，34，4： | 4 |
| 15 | Intrinsic defects in biomass-derived carbons facilitate electroreduction of CO2显示文摘Developing efficient carbon-based metal-free electrocatalysts can bridge the gap between laboratory studies and practical applications of CO2 reduction.However,along with the ambiguous understanding of the active sites in carbon-based electrocatalysts,carbon-based electrocatalysts with high selectivity and satisfactory stability for electroreduction of CO2 remain rare.Here,using the nitrogen rich silk cocoon as a precursor,carbon-based electrocatalysts with intrinsic defects can be prepared for efficient and long-term electroreduction of CO2 by a simple two-step carbonization.The obtained electrocatalyst can catalyze CO2 reduction to CO with a Faradaic efficiency of^89%and maintain good selectivity for about 10 days.Particularly,our experimental studies suggest that in-plane defects are the main active sites on which the rate-determining step for CO2 reduction should be the direct electron transfer to CO2 but not the proton-coupled electron transfer.Further theoretical calculations consistently demonstrate that the intrinsic defects in carbon matrix,particularly the pentagon-containing defects,act as main active sites to accelerate the direct electron transfer for CO2 reduction.In addition,our synthetic approach can convert egg white into efficient catalysts for CO2 electroreduction.These findings,providing new insights into the biomass-derived catalysts,should pave the way for fabricating efficient and stable carbon-based electrocatalysts with catalytically active defects by using naturally abundant precursors. | Mengjie Chen Shuai Wang Haiyan Zhang Ping Zhang Ziqi Tian Min Lu Xiaoji Xie Ling Huang Wei Huang | 2020 | Nano Research2020，13，3： | 4 |
| 16 | Preclinical development of TLR ligands as drugs for the treatment of chronic viral infections显示文摘 | Xiaoyong Zhang Anke Kraft Ruth Broering Joerg F Schlaak Ulf Dittmer Mengji Lu | 2012 | Expert Opinion on Drug Discovery2012，，7： | 3 |
| 17 | Biological porous carbon encapsulated polyethylene glycol-based phase change composites for integrated electromagnetic interference shielding and thermal management capabilities显示文摘The development of functional composites with excellent thermal management capabilities and electro-magnetic interference(EMI)shielding has become extremely urgent for keeping up with the continuous improvement of the operating speed and efficiency for electronic equipment.In this study,the biolog-ical wood-derived porous carbon(WPC)was determined as the supporting material to encapsulating polyethylene glycol(PEG),and a series of WPC/PEG/Fe_(3)O_(4) phase change composites(PCCs)with excel-lent shape stability,EMI shielding and thermal management capabilities were prepared via a simple vac-uum impregnation method.The Fe_(3)O_(4) magnetic particles modified PCCs have greatly improved the EMI shielding effectiveness(SE).The EMI SE of WP-4(7.5 wt.% Fe_(3)O_(4) in PEG)can be up to 55.08 dB between 8.2−12.4 GHz,however,the WP-0 without Fe_(3)O_(4) addition is only 40.08 dB.Meanwhile,the absorption ratio of electromagnetic waves(EMW)has also increased from 75.02%(WP-0)to 85.56%(WP-4),which effectively prevents secondary pollution.In addition,after wrapping a thin layer of polydimethylsiloxane resin(PDMS),the obtained WP-4 can maintain a high heat storage capacity(109.52 J/g)and good wa-ter stability.In short,the prepared WPC/PEG/Fe_(3)O_(4) PCCs have great potential application value in the thermal management and electromagnetic shielding requirements for electronic devices. | Shuang Liu Mengjie Sheng Hao Wu Xuetao Shi Xiang Lu Jinping Qu | 2022 | Journal of Materials Science & Technology2022，，18： | 3 |
| 18 | CRISPR/Cas9-based tools for targeted genome editing and replication control of HBV显示文摘Hepatitis B virus(HBV) infection remains a major global health problem because current therapies rarely eliminate HBV infections to achieve a complete cure. A different treatment paradigm to effectively clear HBV infection and eradicate latent viral reservoirs is urgently required. In recent years, the development of a new RNA-guided gene-editing tool, the CRISPR/Cas9(clustered regularly interspaced short palindromic repeats/CRISPR-associated nuclease 9) system, has greatly facilitated site-specific mutagenesis and represents a very promising potential therapeutic tool for diseases, including for eradication of invasive pathogens such as HBV. Here, we review recent advances in the use of CRISPR/Cas9, which is designed to target HBV specific DNA sequences to inhibit HBV replication and to induce viral genome mutation, in cell lines or animal models. Advantages, limitations and possible solutions, and proposed directions for future research are discussed to highlight the opportunities and challenges of CRISPR/Cas9 as a new, potentially curative therapy for chronic hepatitis B infection. | Cheng Peng Mengji Lu Dongliang Yang | 2015 | Virologica Sinica2015，30，5： | 2 |
| 19 | Role of Toll-like receptor 2 in the immune response against hepadnaviral infection显示文摘 | Xiaoyong Zhang Zhiyong Ma Hongyan Liu Jia Liu Zhongji Meng Ruth Broering Dongliang Yang Joerg F. Schlaak Michael Roggendorf Mengji Lu | 2012 | Journal of Hepatology2012，，3： | 2 |
| 20 | A Case of Hepatitis B Reactivation in an Anti-HBs Positive,Anti-HBc Positive non-Hodgkin’s Lymphoma Patient显示文摘Dear Editor,We report a case of HBV reactivation in an anti-HBs positive, anti-HBc positive non-Hodgkin’s lymphoma patient. Hepatitis B virus (HBV) reactivation is a well-recognized complication of patients undergoing chemotherapy or immunosuppressive therapy for lymphomas. The presence of antibodies to the | Chunchen Wu Hui Shi Mengji Lu Yang Xu Xinwen Chen | 2013 | Virologica Sinica2013，28，1： | 2 |
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