|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Therapeutic effect of autologous dendritic cell vaccine on patients with chronic hepatitis B: A clinical study显示文摘AIM: To investigate the therapeutic effect of autologous HBsAg-loaded dendritic cells (DCs) on patients with chronic hepatitis B.METHODS: Monocytes were isolated from fresh peripheral blood of 19 chronic HBV-infected patients by Ficoll-Hypaque density gradient centrifugation and cultured by plastic-adherence methods. DCs were induced and proliferated in the culture medium with recombinant human granulocyte-macrophage-colony- stimulating factor (rhGM-CSF) and human interleukin-4 (rhIL-4). DCs pulsed with HBsAg for twelve hours were injected into patients subcutaneously twice at intervals of two weeks. Two patients received 100 mg oral lamivudine daily for 12 mo at the same time. HBV-DNA and viral markers in sera of patients were tested every two months.RESULTS: By the end of 2003, 11 of 19 (57.9%) patients had a clinical response to DC-treatment. HBeAg of 10(52.6%) patients became negative, and the copies of HBVDNA decreased 101.77±2.39 averagely (t = 3.13, P<0.01).Two cases co-treated with DCs and lamivudine had a complete clinical response. There were no significant differences in the efficient rate between the cases with ALT level lower than 2xULN and those with ALT level higher than 2xULN before treatment (χ2 = 0.0026).CONCLUSION: Autologous DC-vaccine induced in vitro can effectively suppress HBV replication, reduce the virus load in sera, eliminate HBeAg and promote HBeAg/antiHBe transformation. Not only the patients with high serum ALT levels but also those with normal ALT levels can respond to DC vaccine treatment, and the treatment combining DCs with lamivudine can eliminate viruses more effectively. | MinChen Yong-GuoLi Da-ZhiZhang Zhi-YiWang Wei-QunZeng Xiao-FengShi YuanGuo Shu-HuaGuo HongRen | 2005 | World Journal of Gastroenterology2005，11，12： | 40 |
| 2 | Endoscopic sphincterotomy in the treatment of cholangiopancreatic diseases显示文摘AIM: To investigate the therapeutic effect of endoscopicsphincterotomy (EST) in the treatment of choledocholithiasis and stenosing papillitis.METHODS: A total of 1 026 patients undergoing EST during July 1983 to May 2003 at the institute were retrospectively analyzed. Chronic pancreatitis was diagnosed in 63 (6.1%),cholecystolithiasis and choledocholithiasis in 549 (53.5%),stones in residual biliary duct in 249 (24.3%), stenosing papillitis in 228 (22.2%). In patients with simple stenosing papillitis, most incisions were within 0.5-1 cm in length. As for patients with chronic pancreatitis simultaneously, selective pancreatic sphincterotomy was performed, and incision was within 0.5-0.8 cm in length. For stones less than 1 cm, incision was from 1 to 1.5 cm, and for those larger than 1 cm, incision ranged from 1.5 to 3 cm. For stones more than 2 cm in diameter, detritus basket rather than simple incision was chosen. RESULTS: Of the 798 patients with choledocholithiasis, 764 (93.5%) had successful stone clearance, 215 (94.3%)out of 228 cases of stenosing papillitis were cured totally, while 63 had chronic pancreatitis developed from stenosing papillitis, 57 (90.1%) had sound remission of symptoms, though membranous stenosis emerged in 13 of 57 which was treated with balloon dilatation. After the operation, only 21 cases (2.1%) had complications such as severe pancreatitis and incision bleeding. None of the patients died.CONCLUSION: EST is an ideal surgical management with mini-invasion in the treatment of choledocholithiasis and stenosing papillitis. | Zhi-HuaLi MinChen Ji-KuiLiu JunDing Jia-HongDong | 2005 | World Journal of Gastroenterology2005，11，17： | 23 |
| 3 | A rapid and efficient method to express target genes in mammalian cells by baculovirus显示文摘AIM: To investigate the modification of baculovirus vector and the feasibility of delivering exogenous genes into mammalian cells with the culture supernatant of Spodoptera frugiperta (Sf9) cells infected by recombinant baculoviruses. METHODS: Two recombinant baculoviruses (BacV-CMV-EGFPA, BacV-CMV-EGFPB) containing CMV-EGFP expression cassette were constructed. HepG2 cells were directly incubated with the culture supernatant of Sf9 cells infected by recombinant baculoviruses, and reporter gene transfer and expression efficiencies were analyzed by flow cytometry (FCM). The optimal transduction conditions were investigated by FCM assay in HepG2 cells. Gene-transfer and expression efficiencies in HepG2 or CV1 cells by baculovirus vectors were compared with lipofectAMINE, recombinant retrovirus and vaccinia virus expression systems. Twenty different mammalian cell lines were used to investigate the feasibility of delivering exogenous genes into different mammalian cells with the culture supernatant of infected Sf9 cells. RESULTS: CMV promoter could directly express reporter genes in Sf9 cells with a relatively low efficiency. Target cells incubated with the 1:1 diluted culture supernatant (moi=50) for 12 h at 37 ℃ could achieve the highest transduction and expression efficiencies with least impairment to cell viability. Under similar conditions the baculovirus vector could achieve the highest gene-transfer and expression efficiency than lipofectAMINE, recombinant retrovirus and vaccinia virus expression systems. Most mammalian cell lines could be transduced with recombinant baculovirus. In primate adherent culture cells the recombinant baculovirus could arrive the highest infection and expression efficiencies, but it was not very satisfactory in the cell lines from mice and suspended culture cells. CONCLUSION: Mammalian cells incubated with the culture supernatant of infected Sf9 cells could serve as a very convenient way for rapid and efficient expression of foreign genes in mammalian cells, but it might be more suitable for primate adherent culture cells. | TongCheng Chen-YuXu Ying-BinWang MinChen TingWu JunZhang Ning-ShaoXia | 2004 | World Journal of Gastroenterology2004，10，11： | 7 |
| 4 | Hydrophobicity of reactive site loop of SCCA1 affects its binding to hepatitis B virus显示文摘AIM: To investigate the role of SCCA2 and other SCCA1 molecules in the process of hepatitis B virus (HBV) binding to mammalian cells.METHODS: SCCA1 and SCCA2 were isolated from HepG2. Binding protein (BP) genes were obtained through PCR. Recombinant baculoviruses expressing SCCA1, SCCA2, BP, and different mutants were constructed and utilized to infect mammalian cells to investigate the binding ability of infected cells to HBV.RESULTS: A SCCA1 gene (A1) was isolated from HepG2, but it appeared to lack the binding ability of infected cells to HBV. Two mutants, A1-BP and BP-A1, were constructed by interchanging the carboxyl terminal of A1 and BP. Cells expressing A1-BP showed an increased virus bindingcapacity, but not BP-A1. Comparison of A1 sequence with the sequence of BP indicated the presence of only three amino acid changes in the carboxyl terminal, two of them were found in the reactive site loop (RSL) of SCCA1. Primary structure assay revealed that the hydrophobicity of BP and AJ515706 in this domain was strong, but A1 was relatively weak. Changing the aa349 of A1 from low hydrophobic glutamic acid to high hydrophobic valine enhanced HBV binding. In contrast, HBV binding was reduced by changing the aa349 of BP from valine to glutamic acid. CONCLUSION: The reslts suggest that the hydrophobicity of RSL of SCCA1 may play an important role in HBV binding to cells. | MinChen TongCheng Chen-YuXu TingWu Shan-HaiOu TaoZhang JunZhang Ning-ShaoXia | 2005 | World Journal of Gastroenterology2005，11，19： | 4 |
| 5 | Expression and immunoactivity of chimeric particulate antigens of receptor binding site-core antigen of hepatitis B virus显示文摘AIM: To improve the immunogenicity of receptor binding site of hepatitis B virus (HBV) on preS1 antigen using HBV core antigen as an immuno-carrier.METHODS: One to 6 tandem copies of HBV preS1 (21-47)fragment were inserted into HBcAg at the sites of aa 78 and 82, and expressed in E. coli. ELISA, Western blot and animal immunization were used to analyze the antigenicity and immmunogenicity of purified particulate antigens. The ability to capture HBV by antibodies elicited by chimeric partides was detected with immuno-capture PCR.RESULTS: Recombinant antigens CⅠ, CⅡ, CⅢ carrying 1-3 copies of HBV preS1 (21-47) individually could form viruslike particles (VLPs), similar to HBcAg in morphology. But recombinant antigens carrying 4-6 copies of HBV preS1 (21-47) were poorly expressed in E.coli. Chimeric antigens were lacking of immunoreactivity with anti-HBc monoclonal antibodies (McAbs), but still reserved good immunoreactivity with anti-HBe McAbs. CⅠ, CⅡ, CⅢ could strongly react with anti-preS1 McAb, suggesting that preS1 (21-47) fragment was well exposed on the surface of chimeric VLPs. Three chimeric VLP antigens (CⅠ, CⅡ and CⅢ) could stimulate mice to produce high-level antibody responses, and their immunogenicity was stronger than non-particulate antigen 21-47*6, containing 6 copies of preS1 (21-47). Mouse antibodies to CⅠ, CⅡ and CⅢ were able to capture HBV virions in immuno-capture PCR assay in vitro.CONCLUSION: Chimeric particulate antigens of receptor binding site-core antigen of HBV can elicit strong antibody responses to preS1. They have a potential to be developed into prophylactic or therapeutic vaccines against HBV infection. | Hai-JieYang MinChen TongCheng Shui-ZhenHe Shao-WeiLi Bao-QuanGuan Zi-HengZhu YingGu JunZhang Ning-ShaoXia | 2005 | World Journal of Gastroenterology2005，11，4： | 3 |
| 6 | Coal gasification for advanced power generation显示文摘 | Minchener A J | 2005 | Fuel2005，84，10： | 1 |
| 7 | Coal gasification for advanced powergeneration显示文摘 | MINCHENER A J | 2005 | Fuel2005，84，17： | 1 |
| 8 | The resistive switching characteristics in TaON films for nonvolatile memory applications 显示文摘 | Chen Minchen Chang Tingchang Chiu Yichieh | 2013 | Thin Solid Films2013，528，： | 1 |
| 9 | Coal Gasification for Advanced Power Generation显示文摘 | Minchene A J | 2005 | Fuel2005，84，17： | 1 |
| 10 | Coal gasification for advanced power generation显示文摘 | MINCHENER A J | 2005 | Fuel2005，84，17： | 1 |
| 11 | Coal gasification for advanced power generation显示文摘 | Minchener A J | 2005 | Fuel2005，84，17： | 1 |
| 12 | Subdomain Model for Predicting Armature Reaction Field of Dual-Stator Consequent-Pole PM Machines Accounting for Tooth-Tips显示文摘This paper presents an exact analytical subdomain model of dual-stator consequent-pole permanent-magnet(DSCPPM)machines accounting for tooth-tips,which can accurately predict the armature reaction field distribution in DSCPPM machines.In the proposed subdomain model,the field domain is composed of four types of sub-regions,viz.magnets,outer/inner air gaps,slots and slot openings.The analytical expressions of vector potential in each sub-region are determined by boundary and interface conditions.In comparison to the analytically predicted results,the corresponding flux density field distributions computed by finite element(FE)method are analyzed,which confirms the excellent accuracy of the developed subdomain model. | Minchen Zhu Lijian Wu Youtong Fang Thierry Lubin | 2019 | CES Transactions on Electrical Machines and Systems2019，3，2： | 1 |
| 13 | Enhanced oral bioavailability of silybin by a supersaturatable self-emulsifying drug delivery system (S-SEDDS)显示文摘 | Yinghui Wei Xiaoli Ye Xiaoguang Shang Xuan Peng Qiang Bao Minchen Liu Manman Guo Fanzhu Li | 2011 | Colloids and Surfaces A: Physicochemical and Engineering Aspects2011，，： | 1 |
| 14 | Coal gasification for advanced power generation显示文摘 | MINCHENER A J | 2005 | Fuel2005，84，10： | 1 |
| 15 | Guidelines for developing effective es-timation of distribution algorithms in solving single machine sched-uling problems显示文摘 | Shih - Hsinchen Min - Chihchen Pei - Channchang QingFuzhang Yuh - Minchen | 2010 | Expert Systems with Applications2010，37，9： | 1 |
| 16 | Coal Gasification for Advanced Power Generation 显示文摘 | Minchener A J | 2005 | Fuel2005，84，10： | 1 |
| 17 | Role of bile reflux and Helicobacter pylori infection on inflammation of gastric remnant after distal gastrectomy显示文摘 | Xiao BoLI HongLU Hui MinCHEN Xiao YuCHEN Zhi ZhengGE | 2008 | Journal of Digestive Diseases2008，，4： | 1 |
| 18 | Hybrid coal-fired power plants with CO 2 capture: A technical and economic evaluation based on computational simulations显示文摘 | Y. Huang M. Wang P. Stephenson S. Rezvani D. McIlveen-Wright A. Minchener N. Hewitt A. Dave A. Fleche | 2010 | Fuel2010，，： | 1 |
| 19 | Nitrogen‐Doped Porous Carbon Nanofiber Webs as Anodes for Lithium Ion Batteries with a Superhigh Capacity and Rate Capability显示文摘 | LongQie Wei‐MinChen Zhao‐HuiWang Qing‐GuoShao XiangLi Li‐XiaYuan Xian‐LuoHu Wu‐XingZhang Yun‐HuiHuang | 2012 | Adv Mater2012，，15： | 1 |
| 20 | Coal gasification for advanced power generation 显示文摘 | Minchener A J | 2005 | Fuel2005，84，10： | 1 |
您所在的机构暂未开通此部分的功能，请联系管理员