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| 1 | Schistosoma mansoni proteins attenuate gastrointestinal motility disturbances during experimental colitis in mice显示文摘AIM:To investigate the therapeutic effect of Schistosoma mansoni(S.mansoni) soluble worm proteins on gastrointestinal motility disturbances during experimental colitis in mice. METHODS:Colitis was induced by intrarectal injection of trinitrobenzene sulphate(TNBS) and 6 h later,mice were treated ip with S.mansoni proteins.Experiments were performed 5 d after TNBS injection.Inflammationwas quantified using validated inflammation parameters. Gastric emptying and geometric center were measured to assess in vivo gastrointestinal motility.Peristaltic activity of distal colonic segments was studied in vitro using a modified Trendelenburg set-up.Cytokine profiles of T-lymphocytes isolated from the colon were determined by real time reverse transcriptase-polymerase chain reaction. RESULTS:Intracolonic injection of TNBS caused severe colitis.Treatment with S.mansoni proteins significantly ameliorated colonic inflammation after 5 d.TNBS did not affect gastric emptying but significantly decreased the geometric center and impaired colonic peristaltic activity 5 d after the induction of colitis.Treatment with S.mansoni proteins ameliorated these in vivo and in vitro motility disturbances.In addition,TNBS injection caused a downregulation of effector T cell cytokines after 5 d,whereas a S.mansoni protein effect was no longer observed at this time point. CONCLUSION:Treatment with S.mansoni proteins attenuated intestinal inflammation and ameliorated motility disturbances during murine experimental colitis. | Nathalie E Ruyssers Benedicte Y De Winter Joris G De Man Natacha D Ruyssers Ann J Van Gils Alex Loukas Mark S Pearson Joel V Weinstock Paul A Pelckmans Tom G Moreels | 2010 | World Journal of Gastroenterology2010，16，6： | 11 |
| 2 | Gelatin degradation assay reveals MMP-9 inhibitors and function of O-glycosylated domain显示文摘AIM: To establish a novel, sensitive and high-throughput gelatinolytic assay to define new inhibitors and compare domain deletion mutants of gelatinase B/matrix metalloproteinase (MMP)-9. METHODS: Fluorogenic Dye-quenched (DQ)TM-gelatin was used as a substrate and biochemical parameters (substrate and enzyme concentrations, DMSO solvent concentrations) were optimized to establish a highthroughput assay system. Various small-sized libraries (ChemDiv, InterBioScreen and ChemBridge) of hetero-cyclic, drug-like substances were tested and compared with prototypic inhibitors. RESULTS: First, we designed a test system with gelatin as a natural substrate. Second, the assay was validated by selecting a novel pyrimidine-2,4,6-trione (barbitu- rate) inhibitor. Third, and in line with present structural data on collagenolysis, it was found that deletion of the O-glycosylated region significantly decreased gelatinolytic activity (kcat/kM ± 40% less than full-length MMP-9). CONCLUSION: The DQTM-gelatin assay is useful in high-throughput drug screening and exosite targeting. We demonstrate that flexibility between the catalytic and hemopexin domain is functionally critical for gelatinolysis. | Jennifer Vandooren Nathalie Geurts Erik Martens Philippe E Van den Steen Steven De Jonghe Piet Herdewijn Ghislain Opdenakker | 2011 | World Journal of Biological Chemistry2011，2，1： | 3 |
| 3 | Liver angiogenesis as a risk factor for hepatocellularcarcinoma development in hepatitis C virus cirrhotic patients显示文摘AIM: To evaluate the predictive value of hepatocyte proliferation and hepatic angiogenesis for the occurrence of Hepatocellular carcinoma (HCC) in hepatitis C virus (HCV) cirrhotic patients. METHODS: One hundred-five patients (69 males, 36 females; age range, 51-90 year; median 66 year) with biopsy proven HCV cirrhosis were prospectively monitored for HCC occurrence for a median time of 64 mo. Angiogenesis was assessed by using microvessel density (MVD), hepatocyte turnover by MIB1 and PCNA indexes at inclusion in liver biopsies. RESULTS: Forty six patients (43.8%) developed HCC after a median time of 55 (6-120) mo while 59 (56.2%) did not. Patients were divided into two groups according to the median value of each index. The difference between patients with low (median MVD = 3; range 0-20) and high (median MVD = 7; range 1-24) MVD was statistically significant (χ2 = 22.06; P < 0.0001) which was not the case for MIB1 or PCNA (MIB-1: χ2 = 1.41; P = 0.2351; PCNA: χ2 = 1.27; P = 0.2589). The median MVD was higher in patients who developed HCC than in those who did not. HCC-free interval was significantly longer in patients with the MVD ≤ 4 (P = 0.0006). No relationship was found between MIB1 or PCNA and MVD (MIB-1 r2 = 0.00007116, P = 0.9281; PCNA: r2 =0.001950; P = 0.6692). MVD only was able to predict the occurrence of HCC in these patients. Among other known risk factors for HCC, only male sex was statistically associated with an increased risk. CONCLUSION: Liver angiogenesis has a role for in HCV- related liver carcinogenesis and for defining patients at higher risk. | Roberto Mazzanti Luca Messerini Camilla E Comin Lorenzo Fedeli Nathalie Gannè-Carrie Michel Beaugrand | 2007 | World Journal of Gastroenterology2007，13，37： | 3 |
| 4 | High cardiovascular risk in severely obese young children and adolescents显示文摘 | Nathalie M A van Emmerik Carry M Renders Marije van de Veer Stef van Buuren Olga H van der Baan-Slootweg Joana E Kist-van Holthe Remy A HiraSing | 2012 | Archives of Disease in Childhood2012，，9： | 2 |
| 5 | Melatonin inhibits endothelial nitric oxide production in vitro显示文摘 | Tamra E Werbowetski O Nathalie YR | 2006 | Cancer Res2006，66，3： | 1 |
| 6 | Effect of pKa on the kinetics of carbon dioxide absorption in aqueous alkanolamine solutions containing carbonic anhydrase at 298 K 显示文摘 | Nathalie J M C Penders-van E Sylvie F | 2015 | Chemical Engineering Journal2015，259，1： | 1 |
| 7 | Crohn disease-associated adherent-invasive E. coli bacteria target mouse and human Peyer’s patches via long polar fimbriae显示文摘 | Chassaing Benoit Rolhion Nathalie de Vallée Amélie Salim Sa’ad Y Prorok-Hamon Maelle Neut Christel Campbell Batty J S?derholm Johan D Hugot Jean-Pierre Colombel Jean-Frédéric Darfeuille-Michaud Arlette | 2011 | Journal of Clinical Investigation2011，，3： | 1 |
| 8 | Role for matrix metalloproteinase-2 in oxidized low-density lipoprotein-induced activation of the sphingomyelin/ceramide pathway and smooth muscle cell proliferation显示文摘 | Nathalie A Francoise MS Meyer E | 2004 | Circulation2004，110，3： | 1 |
| 9 | Cytokines: Important for implantation?显示文摘 | Gérard Chaouat Sylvie Dubanchet Nathalie Ledée | 2007 | Journal of Assisted Reproduction and Genetics2007，，11： | 1 |
| 10 | High level of burnout in intensivists: prevalence and associated factors 显示文摘 | NATHALIE E ELIE A KARINE B | 2007 | Am J Respir Crit Care Med2007，175，7： | 1 |
| 11 | Low molecular weight heparin loaded pH-sensitive microparticles 显示文摘 | YVETTE M NATHALIE U FATIMA E G | 2007 | Int J Pharm2007，335，12： | 1 |
| 12 | Modeling vertical and lateral seed bank movements during moule board ploughing显示文摘 | NATHALIE C JEAN R E BRUNO C | 2000 | European Journal of Agronomy2000，13，23： | 1 |
| 13 | Characterization of the chicken fatty acid synthase gene 5' part and promoter region显示文摘 | Nathalie L F Catherine E K Powell R S | 1996 | Eur J Biochem1996，240，： | 1 |
| 14 | Biofiltration of xylene emissions: bioreactor response to variations in the pollutant inlet concentration and gas flow rate 显示文摘 | Hicham E Nathalie B Zarook S | 2004 | Chem Eng J2004，100，13： | 1 |
| 15 | Analysis of the volatile profile and identification of odour-active compounds in Bayonne ham显示文摘 | Laetitia Th e ron Pascal Tournayre Nathalie Kondjoyan | 2010 | Meat Science2010，85，3： | 1 |
| 16 | AmpC cephalosporinase hyperproduction in Acinetobacter baumannii clinical strains 显示文摘 | Stephane C Nathalie C Eric E | 2003 | J Antimicrob Chemother2003，52，4： | 1 |
| 17 | AmpC cephalosporinase hyperproduction in Acinetobacter baumannii clinical strains显示文摘 | Stephane C Nathalie C Eric E | 2003 | J Antimicrob Chemother2003，52，4： | 1 |
| 18 | Heat shock protein-peptide complexes,reconstituded in vitro,elicit peptide-specific cytotoxic T lymphocyte response and tumor immunity显示文摘 | Nathalie E Blachere Z Zihai L | 1997 | J Exp Med1997，8，： | 1 |
| 19 | A polymorphism (80G-A) in the reduced date earner gene and its association with folate status and homocysteinemia显示文摘 | Abalo C Nathalie E F Genevieve P C | 2000 | Mol Gene M etab2000，1070，6： | 1 |
| 20 | Formalin Application in the Treatment of Chronic Radiation-Induced Hemorrhagic Proctitis—An Effective But Not Risk-Free Procedure: A Prospective Study of 33 Patients显示文摘 | Vincent Parades M.D. Isabelle Etienney M.D. Pierre Bauer M.D. Josée Bourguignon M.D. Nathalie Meary M.D. Benoit Mory M.D. Samy Sultan M.D. Milad Taouk M.D. Christian Thomas M.D. Patrick Atienza M.D | 2005 | Diseases of the Colon & Rectum2005，，8： | 1 |
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