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| 1 | Genome editing with RNA-guided Cas9 nuclease in Zebrafish embryos显示文摘有类型 II 的最近的进展定期聚类 interspaced 短 palindromic 重复(CRISPR ) 系统诺言染色体编辑的一条改进途径。然而,在模型有机体的这个系统的适用性和效率例如 zebrafish,几乎没被学习。这里,我们报导那指导 RNA 的 Cas9 核酸酶高效地便于染色体以一种简单、柔韧的方式在哺乳动物的房间和 zebrafish 胚胎编辑。当特定的 Cas/gRNA 被用来在 vivo 在 zebrafish 胚胎指向任何一个 etsrp, gata4 或 gata5 时,超过 35% 地点特定的体的变化被发现。Cas9/gRNA 高效地在结果导致了 etsrp 或 gata5 的 biallelic 变换体的房间,在 etsrp 在 fau 异种胚胎或贲门 bifida 显型 > tm236a 异种胚胎。最后,我们成功地完成了 Cas9/gRNA 系统在 zebrafish 胚胎导致的 mloxP 顺序的地点特定的插入。这些结果证明 Cas9/gRNA 系统有为 zebrafish 和另外的模型有机体成为一个简单、柔韧、有效的反向的基因工具的潜力。和另外的染色体工程技术, Cas9 系统为在生物学,农业,环境研究和药的应用是有希望的。 | Nannan Chang Changhong Sun Lu Gao Dan Zhu Xiufei Xu Xiaojun Zhu Jing-Wei Xiong Jianzhong Jeff Xi | 2013 | Cell Research2013，23，4： | 155 |
| 2 | Hydrogen peroxide primes heart regeneration with a derepression mechanism显示文摘当成年的人的心很限制了再生潜力时,成年 zebrafish 心能充分在 20% 室的切除术以后再生。尽管以前的报告建议象 FGF 和 PDGF 那样的发展发信号小径在成年的心新生被再使用,内在的细胞内部的机制仍然保持大部分未知。这里,我们显示出那 H 2 O 2 充当新奇 epicardial 和心肌的信号在成年 zebrafish 为新生告知心。未经触动的心的实时成像揭示了高度局部性的 H 2 O 2(~ 30 μ M ) 在在切除术地点的心外膜和邻近的紧缩的心肌层的生产。减少的 H 2有 Duox 禁止者 diphenyleneiodonium (点每英寸)或 apocynin 的 O 2形成,或清除 H 2当时,由过氧化氢酶 overexpression 的 O 2显著地损害了心脏的新生外长的 H 2 O 2在心脏的新生上救了点每英寸的禁止的效果,显示那 H 2 O 2是在这个过程的一个必要、足够的信号。机械学地,提高了 H 2 O 2 使动摇氧化还原作用敏感的磷酸酶 Dusp6 并且因此增加了 Erk1/2 的 phosphorylation。当 dusp6 的转基因的 overexpression 损害了心脏的新生时, Dusp6 禁止者 BCI 完成了类似的支持 regenerative 效果。H 2 O 2 在招募起一个双作用通过二条相对独立的小径的有免疫力的房间和支持的心新生。我们结束那 H 2 潜在地从 Duox/Nox2 产生的 O 2 由解开通过包含 Dusp6 的 derepression 机制发信号的地图 kinase 在 zebrafish 支持心新生。 | Peidong Han Xiao-Hai Zhou Nannan Chang Cheng-Lu Xiao Shouyu Yah He Ren Xin-Zhuang Yang Mei-Ling Zhang Qing Wu Boyang Tang Ju-Peng Diao Xiaojun Zhu Chuanmao Zhang Chuan-Yun Li Heping Cheng Jing-Wei Xiong | 2014 | Cell Research2014，24，9： | 10 |
| 3 | Heritable/conditional genome editing in C. elegans using a CRISPR-Cas9 feeding system显示文摘 | Pengpeng Liu Lijiang Long Kai Xiong Bo Yu Nannan Chang Jing-Wei Xiong Zuoyan Zhu Dong Liu | 2014 | Cell Research2014，24，7： | 8 |
| 4 | Heritable gene-targeting with gRNA/Cas9 in rats显示文摘 | Xinli Hu Nannan Chang Xuelian Wang Fengyun Zhou Xiaohai Zhou Xiaojun Zhu Jing-Wei Xiong | 2013 | Cell Research2013，23，11： | 7 |
| 5 | Correlation between LRRK2 gene polymorphism sites S1647T and R1398H and Parkinson’s disease in a Chinese Han population显示文摘A recent multicenter study demonstrated that two variants of LRRK2, S1647T and R1398H, are associated with sporadic Parkinson’s disease. The present study analyzed LRRK2 gene polymorphisms of S1647T and R1398H, demonstrating that the LRRK2 gene polymorphism S1647T variant is a risk factor for Parkinson’s disease in a Chinese Han population. However, the R1398H variant did not influence the risk for Parkinson’s disease. In addition, there was no difference in clinical symptoms of Parkinson’s disease patients with various genotypes. Results showed that the LRRK2 S1647T variant was associated with an increased risk for developing early-onset Parkinson’s disease in a Chinese Han population. In addition, there was no correlation between LRRK2 S1647T, R1398H variants and G2385R, R1628P variants in Parkinson’s disease patients. | Xueli Chang Xueye Mao Zijuan Zhang Jinhong Zhang Yuan Yang Tao Li Nannan Li Jean-Marc Burgunder Rong Peng | 2011 | Neural Regeneration Research2011，6，5： | 4 |
| 6 | Port social and culture survey in the South West of England显示文摘 | Chang YenChiang Wang Nannan Sumser Karen | 2011 | Joumal of Coastal Research2011，27，2： | 1 |
| 7 | ICAM-5/Telencephalin是肠道病毒EV-D68入侵宿主细胞的功能受体显示文摘肠道病毒D68型(EV-D68)是小RNA病毒家族的成员之一。尽管EV-D68病毒此前曾被认为是罕见病毒,但近年来其导致的传染病在世界范围内频繁爆发。 | 魏伟 Haoran Guo Junliang Chang Yingzi Yu Guanchen Liu Nannan Zhang Stephen H.Willard Shu Zheng 于晓方 | 2017 | 科学新闻2017，19，4： | 0 |
| 8 | The presence of foci of Rickettsia conorii infection in China显示文摘China is not considered as an endemic area of Rickettsia conorii,so there is no routine clinical way to diagnose this infection.This study aims to determine whether 2 febrile patients who had a tick bite in East China were indeed infected with R.conorii.The citrate synthase gene(gltA)was amplified with universal rickettsial primers by real-time fluorescent PCR from the patients’blood samples.Nested PCR was used to amplify the outer membrane protein A gene(ompA)for positive specimens.PCR products were further identified and analyzed through nucleic acid sequencing.Positive amplification of the gltA and ompA genes was found in both patients.The nucleotide sequences(303 bp)of the ompA gene of the 2 patients had high homology(99%)with the R.conorii Indian tick typhus strain in GenBank.A more than 4-fold increase in IgG against R.conorii provided supportive evidence of SFG Rickettsia infection.And the rapid recovery after doxycycline treatment also supported a rickettsial cause for the disease.Physicians in East China should be aware of human infections with R.conorii.PCR-based diagnostic methods offer a rapid and precise way to diagnose rickettsiosis,improving patient identification and management. | Nannan Xu Hui Liu Chunmei Qu Sai Wen Wenlu Zou Caiyun Chang Gang Wang | 2023 | Infectious Medicine2023，2，4： | 0 |
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