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| 1 | Pristine mesoporous carbon hollow spheres as safe adjuvants induce excellent Th2-biased immune response显示文摘放大有免疫力的反应到抗原的一个安全、有效的助手的发展为疫苗的交货是重要的。在这研究,我们开发了太古的 mesoporous 碳是的空范围为增加有免疫力的反应的高能力的疫苗的蛋白质 nanocarriers 和安全助手。单音分散入鞘空碳范围(IMHCS ) 有的 mesostructured 200 nm, 15 nm 的大毛孔尺寸,和为 SRCA 的 2.85 厘米 3 的高毛孔体积的一种平均粒子尺寸。以便证实这试金的特性,包括属于不同 Shigella 种类和 23 个 non-Shigella 细菌的 11 个种类的 34 个种类与纯文化被检测。由 SRCA 的 Shigella flexneri 的敏感用 agarose 胶化电气泳动被评估,它是 7.3 ?> 并且 CH 4( 大约 13 ? ktonnd FE-SEM 证明 grafting 是成功的。我们然后证明 grafted 膜用吸附对牛的浆液白朊而且葡聚糖显示出优秀低犯规的性质不仅吗?? | Manasi Jambhrunkar Meihua Yu Hongwei Zhang Prasanna Abbaraju Anand Kumar Meka Antonino Cavallaro Yao Lu Neena Mitter Chengzhong Yu | 2018 | Nano Research2018,11,1: | 4 |
| 2 | Systemic induction of an Arabidopsis plant defensin gene promoter by tobacco mosaic virus and jasmonic acid in transgenic tobacco 显示文摘 | Mitter Neena Kazan | 1998 | Plant Science1998,136,2: | 1 |
| 3 | Tissue culture tools for selenium hyperaccumulator Neptunia amplexicaulis for development in phytoextraction显示文摘Neptunia amplexicaulis is an herbaceous legume endemic to the Richmond area in central Queensland,Australia and is one of the strongest known Selenium hyperaccumulators on earth,showing significant potential to be utilised in Se phytoextraction applications.Here a protocol was established for in vitro micropropagation of Se hyperaccumula-tor N.amplexicaulis using nodal segments from in vitro-germinated seedlings.Shoot multiplication was achieved on Murashige and Skoog(MS)basal media supplemented with various concentrations of 6-Benzylaminopurine(BA)(1.0,2.0,3.0 mg L^(−1))alone or in combination with low levels of Naphthaleneacetic acid(NAA)(0.1,0.2,0.3 mg L^(−1)),with 2.0 mg L^(−1) BA+0.2 mg L^(−1) NAA found to be most effective.Elongated shoots were rooted in vitro using NAA,with highest root induction rate of 30%observed at 0.2 mg L^(−1) NAA.About 95%of the in vitro rooted shoots survived acclimatization.Clonally propagated plantlets were dosed with selenate/selenite solution and assessed for Se tissue concentrations using Inductively Coupled Plasma Atomic Emission Spectroscopy(ICP-AES)and found to retain their ability to hyperaccumulate.The protocol developed for this study has potential to be optimised for generating clonal plants of N.amplexicaulis for use in research and phytoextraction industry applications. | Billy O’Donohue Jayeni Hiti-Bandaralage Madeleine Gleeson Chris O’Brien Maggie-Anne Harvey Antony van der Ent Katherine Pinto Irish Neena Mitter Alice Hayward | 2022 | Natural Products and Bioprospecting2022,12,1: | 0 |
| 4 | Perspectives on plant virus diseases in a climate change scenario of elevated temperatures显示文摘Global food production is at risk from many abiotic and biotic stresses and can be affected by multiple stresses simultaneously.Virus diseases damage cultivated plants and decrease the marketable quality of produce.Importantly,the progression of virus diseases is strongly affected by changing climate conditions.Among climate-changing vari-ables,temperature increase is viewed as an important factor that affects virus epidemics,which may in turn require more efficient disease management.In this review,we discuss the effect of elevated temperature on virus epidem-ics at both macro-and micro-climatic levels.This includes the temperature effects on virus spread both within and between host plants.Furthermore,we focus on the involvement of molecular mechanisms associated with tempera-ture effects on plant defence to viruses in both susceptible and resistant plants.Considering various mechanisms proposed in different pathosystems,we also offer a view of the possible opportunities provided by RNA-based technologies for virus control at elevated temperatures.Recently,the potential of these technologies for topical field applications has been strengthened through a combination of genetically modified(GM)-free delivery nanoplat-forms.This approach represents a promising and important climate-resilient substitute to conventional strategies for managing plant virus diseases under global warming scenarios.In this context,we discuss the knowledge gaps in the research of temperature effects on plant-virus interactions and limitations of RNA-based emerging technologies,which should be addressed in future studies. | Wei‑An Tsai Christopher A.Brosnan Neena Mitter Ralf G.Dietzgen | 2022 | Stress Biology2022,2,1: | 0 |
| 5 | BioClay^(TM)prolongs RNA interference-mediated crop protection against Botrytis cinerea显示文摘One of the most promising tools for the control of fungal plant diseases is spray-induced gene silencing(SIGS).In SIGS,small interfering RNA(siRNA)or double-stranded RNA(dsRNA)targeting essential or virulence-related pathogen genes are exogenously applied to plants and postharvest products to trigger RNA interference(RNAi)of the targeted genes,inhibiting fungal growth and disease.However,SIGS is limited by the unstable nature of RNA under environmental conditions.The use of layered double hydroxide or clay particles as carriers to deliver biologically active dsRNA,a formulation termed BioClay^(TM),can enhance RNA durability on plants,prolonging its activity against pathogens.Here,we demonstrate that dsRNA delivered as BioClay can prolong protection against Botrytis cinerea,a major plant fungal pathogen,on tomato leaves and fruit and on mature chickpea plants.BioClay increased the protection window from 1 to 3 weeks on tomato leaves and from 5 to 10 days on tomato fruits,when compared with naked dsRNA.In flowering chickpea plants,BioClay provided prolonged protection for up to 4 weeks,covering the critical period of poding,whereas naked dsRNA provided limited protection.This research represents a major step forward for the adoption of SIGS as an eco-friendly alternative to traditional fungicides. | Jonatan Niño-Sánchez Prabhakaran T.Sambasivam Anne Sawyer Rachael Hamby Angela Chen Elizabeth Czislowski Peng Li Narelle Manzie Donald M.Gardiner Rebecca Ford Zhi Ping Xu Neena Mitter Hailing Jin | 2022 | Journal of Integrative Plant Biology2022,64,11: | 0 |