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| 1 | Hepatitis B and C virus-induced hepatitis: Apoptosis, autophagy, and unfolded protein response显示文摘AIM: To investigate the co-incidence of apoptosis, autophagy, and unfolded protein response(UPR) in hepatitis B(HBV) and C(HCV) infected hepatocytes.METHODS: We performed immunofluorescence confocal microscopy on 10 liver biopsies from HBV and HCV patients and tissue microarrays of HBV positive liver samples. We used specific antibodies for LC3β, cleaved caspase-3, BIP(GRP78), and XBP1 to detect autophagy, apoptosis and UPR, respectively. AntiHCV NS3 and anti-HBs antibodies were also used to confirm infection. We performed triple blind counting of events to determine the co-incidence of autophagy(LC3β punctuate), apoptosis(cleaved caspase-3), and unfolded protein response(GRP78) with HBV and HCV infection in hepatocytes. All statistical analyses were performed using SPSS software for Windows(Version 16 SPSS Inc, Chicago, IL, United States). P-values < 0.05 were considered statistically significant. Statistical analyses were performed with Mann-Whitney test to compare incidence rates for autophagy, apoptosis, and UPR in HBV- and HCV-infected cells and adjacent noninfected cells.RESULTS: Our results showed that infection of hepatocytes with either HBV and HCV induces significant increase(P < 0.001) in apoptosis(cleavage of caspase-3), autophagy(LC3β punctate), and UPR(increase in GRP78 expression) in the HCV- and HBVinfected cells, as compared to non-infected cells of the same biopsy sections. Our tissue microarray immunohistochemical expression analysis of LC3β in HBV^(Neg) and HBV^(Pos) revealed that majority of HBVinfected hepatocytes display strong positive stainingfor LC3β. Interestingly, although XBP splicing in HBVinfected cells was significantly higher(P < 0.05), our analyses show a slight increase of XBP splicing was in HCV-infected cells(P > 0.05). Furthermore, our evaluation of patients with HBV and HCV infection based on stage and grade of the liver diseases revealed no correlation between these pathological findings and induction of apoptosis, autophagy, and UPR.CONCLUSION: The results of this study indicate that HCV and HBV infection activates apoptosis, autophagy and UPR, but slightly differently by each virus. Further studies are warranted to elucidate the interconnections between these pathways in relation to pathology of HCV and HBV in the liver tissue. | Behzad Yeganeh Adel Rezaei Moghadam Javad Alizadeh Emilia Wiechec Seyed Moayed Alavian Mohammad Hashemi Bita Geramizadeh Afshin Samali Kamran Bagheri Lankarani Martin Post Payam Peymani Kevin M Coombs Saeid Ghavami | 2015 | World Journal of Gastroenterology2015，21，47： | 13 |
| 2 | Effect of porosity on response behavior of carbon black-PMMA conductive composite sensors toward organic vapors显示文摘 | Ehsan D Seyed R G Payam M A | 2009 | Olfaetion and electronic nose:proceedings of the 13th International Symposium on Olfaction and Electronic Nose2009，1137，： | 1 |
| 3 | Using hidden Markov models for paper currency recognition original显示文摘 | Hassanpour H Payam M F | 2009 | Expert Systemswith Applications2009，36，10： | 1 |
| 4 | Combination of dispersive liquid-liquid microextraction with flame atomic absorption spectrometry using microsample introduction for determination of lead in water samples显示文摘 | NASERI M T PAYAM H HOSSEINI M R | 2008 | Anal Chim Acta2008，610，1： | 1 |
| 5 | Tooth movements in foxhounds after one or two alve- olar corticotomies显示文摘 | Payam A Sanjideh R Emile Rossouw Phillip M Campbell eta 1 | 2010 | European Journal of Orthodontics2010，32，1： | 1 |
| 6 | A Global Review ofEnergy Consumption, CO2 Emissions and Policy in the Residential Sector (With an Overview of the Top Ten CO2 Emitting Countries) 显示文摘 | Payam N Fatemeh J Mohammad M T | 2015 | Renewable and Sustainable Energy Reviews2015，43，： | 1 |
| 7 | Value-adding to cellulosic ethanol lignin polymers显示文摘 | WILLIAM O S D PAYAM M CHRISTOPHER M F | 2011 | Queensland University and Technology2011，33，2： | 1 |
| 8 | Seismic risk assessment of four-Span bridges in Montreal designed using the Canadian bridge design code显示文摘 | Payam T Denis M | 2014 | ASCE Journal of Bridge Engineering2014，19，8： | 1 |
| 9 | Design of a smart MEMS accelerometer using nonlinear control principles显示文摘 | Hassani F A Payam A F Fathipour M | 2010 | Smart Structures and Systems2010，6，1： | 1 |
| 10 | Using Hidden Markov Models for Paper Currency Recognition Original显示文摘 | HASSANPOUR H PAYAM M F | | 0，，6： | 1 |
| 11 | Chitosan-phospholipid blend for sustained and localized delivery of docetaxel to the peritoneal cavity显示文摘 | Payam Z Raquel D S Micheline P M | 2009 | Int J Pharm2009，377，12： | 1 |
| 12 | Effect of steel fiber on the mechanical properties of oil palm shell lightweight concrete显示文摘 | PAYAM S HlLMI M MOHD Z J | 2011 | Materials and Design2011，32，7： | 1 |
| 13 | Value-adding to cellulosic ethanol:Lignin polymers显示文摘 | William O S Payam M Christopher M F | | 0，，02： | 1 |
| 14 | A rapid and sensitive method for simultaneous determination of insulin and A21-desamido insulin by high-performance liquid chromatography 显示文摘 | PAYAM M ABDOLHOSSEIN R N HOSNIEH T | 2003 | J Pharm BiornedAnal2003，33，1： | 1 |
| 15 | Using Hidden Markov Models for Paper Currency Recognition Original显示文摘 | Hassanpour H Payam M F | 2009 | Expert Systems with Applications2009，36，10： | 1 |
| 16 | Me- chanical properties of structural lightweight ag- gregate concrete containing low volume steel fiber显示文摘 | MAHMOUD H PAYAM S HILMI B M | 2014 | Journal for science and engineering2014，39，： | 1 |
| 17 | Chemical and Thermal Characterization of Cement Mortar Containing Ground Palm Oil Fuel Ash as a Partial Cement Replacement显示文摘This study investigates the influence of using ground palm oil fuel ash(G-POFA) from 10%-30% as cement replacement(by weight) on the cement mortar's pH under various curing conditions. These findings were supplemented by thermal gravimetric analysis(TGA). Moreover, the resistance of G-POFA blended cement mortars to water absorption and sorptivity was determined. Further, the k-value test was carried out to explain the pozzolanic and filler behavior of G-POFA and to support the results obtained from TGA. It was found that there was no significant impact of several curing conditions on the pH of mortars. The mortar with 10% G-POFA in replacement of cement(G-POFA-10) exhibited the best resistance against water absorption and sorptivity. | SUMRA Yousuf PAYAM Shafigh IFTIKHAR Ahmed Channa M Rizwan TANVEER Ahmed Khan BELAL Alsubari MUSTABSHIRHA Gul | 2023 | Journal of Wuhan University of Technology(Materials Science)2023，38，3： | 0 |
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