|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Essential sequence of the N-terminal cytoplasmic localization-related domain of huntingtin and its effect on huntingtin aggregates显示文摘Huntington's disease(HD) is caused by abnormal CAG repeat expansion in the 5′-end of the Huntingtin(HTT) gene.In addition to the canonical C-terminal full-length huntingtin(htt) nuclear export signal,a cytoplasmic localization-related domain(CLRD) in the N-terminus of htt has recently been reported.Here,we analyzed this domain by introducing deletion and substitution mutations in a truncated N-terminal htt protein and subsequently monitored htt expression,aggregation and subcellular localization by immunocytochemistry and Western blot analysis.We demonstrated that Htt4-17 was the essential sequence for htt cytoplasmic localization.We also found that the subcellular distribution of htt was altered when Htt1-17 was mutated to contain amino acids of different charges,suggesting a structural requirement of Htt1-17 for the cytoplasmic localization of htt. Deletion of the first three amino acids did not affect its association with mitochondria.We observed that defective cytoplasmic localization resulted in a reduction of total htt aggregates and increased nuclear aggregates,indicating that the subcellular distribution of the protein might influence the aggregation process.These studies provide new insight into the molecular mechanism of htt aggregation in HD. | YAN YaPing PENG DanTao TIAN Jun CHI JingWei TAN JieQiong YIN XinZhen PU JiaLi XIA Kun ZHANG BaoRong | 2011 | Science China(Life Sciences)2011，54，4： | 4 |
| 2 | Risk Regionalization of Drought for Flue-cured Tobacco Planting in Qujing City Based on GIS显示文摘The risk regionalization of drought for flue-cured tobacco planting in Qujing City was studied to provide reference for drawing on advantages and avoiding disadvantages in flue-cured tobacco planting,disaster reduction,and disaster relief services.According to the production practice of flue-cured tobacco and local climate analysis,the risk of disaster-causing factors,sensitivity of disaster-breeding environment,vulnerability of carriers,and disaster prevention and reduction capability were analyzed and evaluated quantitatively.Secondly,starting from the formation mechanism of drought,a risk assessment model of the meteorological disaster was established by GIS technology.Finally,the risk assessment and regionalization of drought in the critical periods for drought-stricken flue-cured tobacco (transplanting period,root extending stage and vigorous growth period) in Qujing City were carried out.The results showed that in Qujing City,the risk areas of drought for flue-cured tobacco planting were divided into five grades in the transplanting period and six grades in the root extending stage and vigorous growth period. | Pu Jicun Cheng Jiali Zhang Maosong Yuan Jiafeng Bai Bo | 2019 | Meteorological and Environmental Research2019，10，5： | 1 |
| 3 | Induced neural stem/precursor cells for fundamental studies and potential application in neurodegenerative diseases显示文摘Recent research has shown that defined sets of exogenous factors are sufficient to convert rodent and human somatic cells directly into induced neural stem cells or neural precursor cells(iNSCs/iNPCs).The process of transdifferentiation bypasses the step of a pluripotent state and reduces the risk of tumorigenesis and genetic instability while retaining the self-renewing capacity.This iNSC/iNPC technology has fueled much excitement in regenerative medicine,as these cells can be differentiated into target cells for replacement therapy for neurodegenerative diseases.Patients' somatic cell-derived iNSCs/iNPCs have also been proposed to serve as disease models with potential value in both fundamental studies and clinical applications.This review focuses on the mechanisms,techniques,and applications of iNSCs/iNPCs from a series of related studies,as well as further efforts in designing novel strategies using iNSC/iNPC technology and its potential applications in neurodegenerative diseases. | Ting Shen Jiali Pu Tingting Zheng Baorong Zhang | 2015 | Neuroscience Bulletin2015，31，5： | 1 |
| 4 | cFos-ANAB:A cFos-based Web Tool for Exploring Activated Neurons and Associated Behaviors显示文摘cFos is one of the most widely-studied genes in the field of neuroscience.Currently,there is no systematic database focusing on cFos in neuroscience.We developed a curated database-cFos-ANAB-a cFos-based web tool for exploring activated neurons and associated behaviors in rats and mice,comprising 398 brain nuclei and sub-nuclei,and five associated behaviors:pain,fear,feeding,aggression,and sexual behavior.Direct relationships among behaviors and nuclei(even cell types)under specific stimulating conditions were constructed based on cFos expression profiles extracted from original publications.Moreover,overlapping nuclei and sub-nuclei with potentially complex functions among different associated behaviors were emphasized,leading to results serving as important clues to the development of valid hypotheses for exploring as yet unknown circuits.Using the analysis function of cFos-ANAB,multi-layered pictures of networks and their relationships can quickly be explored depending on users’purposes.These features provide a useful tool and good reference for early exploration in neuroscience.The cFos-ANAB database is available at www.cfos-db.net. | Fan Wang Wenjie Sun Lei Chang Kefang Sun Leying Hou Linna Qian Chaoyin Jin Jiandong Chen Jiali Pu Panmeng Ye Shuang Qiu Jianhong Luo Shumin Duan Baorong Zhang Zhihua Gao Xiaojun Hu | 2021 | Neuroscience Bulletin2021，37，10： | 0 |
| 5 | Oscillation-specific nodal alterations in early to middle stages Parkinson’s disease显示文摘Background:Different oscillations of brain networks could carry different dimensions of brain integration.We aimed to investigate oscillation-specific nodal alterations in patients with Parkinson’s disease(PD)across early stage to middle stage by using graph theory-based analysis.Methods:Eighty-eight PD patients including 39 PD patients in the early stage(EPD)and 49 patients in the middle stage(MPD)and 36 controls were recruited in the present study.Graph theory-based network analyses from three oscillation frequencies(slow-5:0.01–0.027 Hz;slow-4:0.027–0.073 Hz;slow-3:0.073–0.198 Hz)were analyzed.Nodal metrics(e.g.nodal degree centrality,betweenness centrality and nodal efficiency)were calculated.Results:Our results showed that(1)a divergent effect of oscillation frequencies on nodal metrics,especially on nodal degree centrality and nodal efficiency,that the anteroventral neocortex and subcortex had high nodal metrics within low oscillation frequencies while the posterolateral neocortex had high values within the relative high oscillation frequency was observed,which visually showed that network was perturbed in PD;(2)PD patients in early stage relatively preserved nodal properties while MPD patients showed widespread abnormalities,which was consistently detected within all three oscillation frequencies;(3)the involvement of basal ganglia could be specifically observed within slow-5 oscillation frequency in MPD patients;(4)logistic regression and receiver operating characteristic curve analyses demonstrated that some of those oscillation-specific nodal alterations had the ability to well discriminate PD patients from controls or MPD from EPD patients at the individual level;(5)occipital disruption within high frequency(slow-3)made a significant influence on motor impairment which was dominated by akinesia and rigidity.Conclusions:Coupling various oscillations could provide potentially useful information for large-scale network and progressive oscillation-specific nodal alterations were observed in PD patients across early to middle stages. | Xiaojun Guan Tao Guo Qiaoling Zeng Jiaqiu Wang Cheng Zhou Chunlei Liu Hongjiang Wei Yuyao Zhang Min Xuan Quanquan Gu Xiaojun Xu Peiyu Huang Jiali Pu Baorong Zhang Min-Ming Zhang | 2019 | Translational Neurodegeneration2019，8，1： | 0 |
您所在的机构暂未开通此部分的功能，请联系管理员