|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Visualizing the microtubule-associated protein tau in the nucleus显示文摘Although tau is mainly known as an axonal microtubule-associated protein,many studies indicate that it is not restricted to this subcellular compartment.Assessing tau’s subcellular distribution,however,is not trivial as is evident from transgenic mouse studies.When human tau is over-expressed,it can be immunohistochemically localized to axons and the somatodendritic domain,modeling what is found in neurodegenerative diseases such as Alzheimer’s disease.Yet,in wild-type mice,despite its abundance,tau is difficult to visualize even in the axon.It is even more challenging to detect this protein in the nucleus,where tau has been proposed to protect DNA from damage.To establish a framework for future studies into tau’s nuclear functions,we compared several methods to visualize endogenous nuclear tau in cell lines and mouse brain.While depending on the fixation and permeabilization protocol,we were able to detect nuclear tau in SH-SY5Y human neuroblastoma cells,we failed to do so in N2a murine neuroblastoma cells.As a second method we used subcellular fractionation of mouse tissue and found that in the nucleus tau is mainly present in a hypophosphorylated form.When either full-length or truncated human tau was expressed,both accumulated in the cytoplasm,but were also found in the nuclear fraction.Because subcellular fractionation methods have their limitations,we finally isolated nuclei to probe for nuclear tau and found that the nuclei were free of cytoplasmic contamination.Together our analysis identifies several protocols for detecting tau in the nucleus where it is found in a less phosphorylated form. | LU Jing LI Ting HE RongQiao BARTLETT Perry F GTZ Jürgen | 2014 | Science China(Life Sciences)2014，57，4： | 11 |
| 2 | Contextual Factors as Moderators of the Effect of Employee Ethical Ideology on Ethical Decision-making 显示文摘 | Callanan G A Rotenberry P F Perri D F | 2010 | International Journal of Management2010，27，1： | 1 |
| 3 | Renal expression and localization of SLC9A3 sodium/hydrogen exchanger and its possible role in acid-base regulation in freshwater rainbow trout ( Oncorhynchus mykiss ) 显示文摘 | IVANIS G BRAUN M PERRY S F | 2008 | American Journal of Physiology: Regulatory Integrative and Comparative Physiology2008，295，3： | 1 |
| 4 | Ipilimumab显示文摘 | Cameron F Whiteside G Perry C | 2011 | Drugs2011，71，8： | 1 |
| 5 | Trophody- namic and advective influences on Georges Bank larval cod and haddock 显示文摘 | Werner F E Perry R I Lough R G | 1996 | Deep-Sea Research II1996，43，： | 1 |
| 6 | Apparent metabolizable energy values of fats for broiler chicks显示文摘 | Wiseman J D Cole J A Perry F G | 1986 | Br Poult Sci1986，27，： | 1 |
| 7 | Characterization of branchial transepithelial calcium fluxes in freshwater trout, Salmo gairdneri 显示文摘 | Perry S F Flik G | 1988 | Am J Physiol Regul Integr Comp Physiol1988，254，： | 1 |
| 8 | Relationship between follicle size at insemination and pregnancy success 显示文摘 | PERRY G A SMITH M F LUCY M C | 2005 | PNAS2005，102，14： | 1 |
| 9 | The effect of rapid thermal processing (RTP) on TiN coatings deposited by PVD and the steel-turning performance of coated cemen- ted carbide 显示文摘 | Anthoy J Perry J N Steve J Bull Dmitri I P Peter C R Trevor F P Daniel E G James T John J V Robert E D Vladinmir R Alexey B M | 1999 | Surface and Coatings Technology1999，120，121： | 1 |
| 10 | Ipilimumab in the treatment of metastatic melanoma: management of adverse events显示文摘 | Delia Vittoria Scarpati G Fusciello C Perri F | 2014 | Onco Targets Ther2014，7，： | 1 |
| 11 | A mathematical model for photopolymerization from a stationary laser light source 显示文摘 | MICHAEL F PERRY G YOUNG W | 2005 | Macromol Theor Simul2005，14，： | 1 |
| 12 | Dynamic arch in free-flowing granular material discharging from a model hopper显示文摘 | Perry M G Handley M F | 1967 | Transactions of the Institution of Chemical Engineers and the Chemical Engineer1967，45，9： | 1 |
| 13 | Sedimentary evolution of the Mesozoic continental redbeds using geochemical and mineralogical tools:The case of Upper Triassic to Lowermost Jurassic Monte di Gioiosa mudrocks(Sicily,southern Italy)显示文摘 | Perri F Critelli S Mongelli G | 2011 | International Journal of Earth Sciences2011，100，： | 1 |
| 14 | Helicob acter pylori infection and growth delay in older children 显示文摘 | Perri F Pastore M Leandro G | 1997 | Arch Dis Child1997，77，1： | 1 |
| 15 | Trophodynamic and advective influences on Georges Bank larval cod and haddock 显示文摘 | WERNER F E Perry R I Lough R G | 1996 | Deep Sea Research1996，，43： | 1 |
| 16 | Urinary free (uneon- jugated) metadrenalines in different hereditary forms of eate- eholamine-seereting phaeoehromoeytoma/paraganglioma显示文摘 | Davidson D F Bradshaw N Perry C G | 2012 | Ann Clin Bioehem2012，49，5： | 1 |
| 17 | Information-theory measure of uncertainty for rough sets and rough relational databases显示文摘 | Beaubouef T Perry F E Arona G | 1998 | Inform Sci1998，109，： | 1 |
| 18 | Mosaic HIV-1vaccines expand the breadth and depth of cellular immune responses in rhesus monkeys显示文摘 | Barouch D H O'Brien K L Simmons N L King S L Abbink P Maxfield L F Sun Y H La Porte A Riggs A M Lynch D M Clark S L Backus K Perry J R Seaman M S Carville A Mansfield K G Szinger J J Fischer W Muldoon M Korber B | 2010 | Nat Med2010，16，3： | 1 |
| 19 | A design method for PI- like fuzzy logic controllers for DC-DC converter显示文摘 | PERRY A G FENG G LIU Y F | 2007 | IEEE Transactions on Industrial Electronics2007，54，5： | 1 |
| 20 | New treatment approaches in renal cell carcinoma 显示文摘 | Facchini G Perri F Caraglia M | 2009 | Anticancer Drugs2009，20，10： | 1 |
您所在的机构暂未开通此部分的功能，请联系管理员