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| 1 | A Strigolactone Biosynthesis Gene Contributed to the Green Revolution in Rice显示文摘Plant architecture is a complex agronomic trait and a major factor of crop yield,which is affected by several important hormones.Strigolactones(SLs)are identified as a new class hormoneinhibiting branching in many plant species and have been shown to be involved in various developmental processes.Genetical and chemical modulation of the SL pathway is recognized as a promising approach to modify plant architecture.However,whether and how the genes involved in the SL pathway could be utilized in breeding still remain elusive.Here,we demonstrate that a partial loss-of-function allele of the SL biosynthesis gene,HIGH TILLERING AND DWARF 1/DWARF17(HTD1/D17),which encodes CAROTENOID CLEAVAGE DIOXYGENASE 7(CCD7),increases tiller number and improves grain yield in rice.We found that the HTD1 gene had been widely utilized and co-selected with Semidwarf 1(SD1),both contributing to the improvement of plant architecture in modern rice varieties since the Green Revolution in the 1960s.Understanding how phytohormone pathway genes regulate plant architecture and how they have been utilized and selected in breeding will lay the foundation for developing the rational approaches toward improving crop yield. | Yuexing Wang Lianguang Shang Hong Yu Longjun Zeng Jiang Hu Shen Ni Yuchun Rao Sanfeng Li Jinfang Chu Xiangbing Meng Lei Wang Ping Hu Jijun Yan Shujing Kang Minghao Qu Hai Lin Tao Wang Quan Wang Xingming Hu Hongqi Chen Bing Wang Zhenyu Gao Longbiao Guo Dali Zeng Xudong Zhu Guosheng Xiong Jiayang Li Qian Qian | 2020 | Molecular Plant2020，13，6： | 23 |
| 2 | Role of PERK/eIF2α/CHOP Endoplasmic Reticulum Stress Pathway in Oxidized Low-density Lipoprotein Mediated Induction of Endothelial Apoptosis显示文摘Objective PERK/eI F2α/CHOP is a major signaling pathway mediating endoplasmic reticulum(ER) stress related with atherosclerosis.Oxidized LDL(ox-LDL) also induces endothelial apoptosis and plays a vital role in the initiation and progression of atherosclerosis.The present study was conducted to explore the regulatory effect of ox-LDL on PERK/e IF2α/CHOP signaling pathway in vascular endothelial cells.Methods The effects of ox-LDL on PERK and p-e IF2α protein expression of primary human umbilical vein endothelial cells(HUVECs) were investigated by Western blot analysis.PERK gene silencing and selective eI F2α phosphatase inhibitor,salubrinal were used to inhibit the process of ox-LDL induced endothelial cell apoptosis,caspase-3 activity,and CHOP mR NA level.Results Ox-LDL treatment significantly increased the expression of PERK,PERK-mediated inactivation of e IF2α phosphorylation,and the expression of CHOP,as well as the caspase-3 activity and apoptosis.The effects of ox-LDL were markedly decreased by knocking down PERK with stable transduction of lentiviral sh RNA or by selective eI F2α phosphatase inhibitor,salubrinal.Conclusion This study provides the first evidence that ox-LDL induces apoptosis in vascular endothelial cells mediated largely via the PERK/eI F2α/CHOP ER-stress pathway.It adds new insights into the molecular mechanisms underlying the pathogenesis and progression of atherosclerosis. | TAO Yong Kang YU Pu Lin BAI Yong Ping YAN Sheng Tao ZHAO Shui Ping ZHANG Guo Qiang | 2016 | Biomedical and Environmental Sciences2016，29，12： | 21 |
| 3 | A Tentative Research on Complexity of Automatic Programming显示文摘In this paper, based on the following theoretical framework: Evolutionary Algorithms + Program Structures = Automatic Programming , some results on complexity of automatic programming for function modeling is given, which show that the complexity of automatic programming is an exponential function of the problem dimension N , the size of operator set |F| and the height of the program parse tree H . Following this results, the difficulties of automatic programming are discussed. Some function models discovered automatically from database by evolutionary modeling method are given, too. | Kang Li\|shan, Li Yan, Chen Yu\|ping Computation Center, State Key Laboratory of Software Engineering,Wuhan University,Wuhan 430072,China | 2001 | Wuhan University Journal of Natural Sciences2001，6，Z1： | 18 |
| 4 | Asymptomatic patients of chronic obstructive pulmonary disease in China显示文摘背景长期的妨碍的肺的疾病(COPD ) 让可变自然历史和并非所有个人跟随一样的功课。这研究试图在 China.Methods 从基于人口的调查识别无征状的 COPD 病人的流行和特征多级式的簇采样策略从七不同省 / 城市在一张人口被使用。所有居民(超过 40 岁) 与一张标准化问询表被会见, spirometry.Post-bronchodilator 在 1 秒(FEV1 ) 强迫了吐气的体积 /forced 不到 70% 的重要能力(FVC ) 被定义为 COPD 的诊断标准。屏蔽的所有 COPD 病人根据长期的呼吸症状的存在或缺席被划分成征兆的组和无征状的组。Socio 人口统计,个人和暴露变量被收集,在从 25 627 采样与 COPD 被诊断的 1668 个病人之中的 analyzed.Results 使遭到,(35.3%) 589 是无征状的。年龄,性别,身体团索引(BMI ) ,农村、城市的分布,吸烟习惯和教育层次在二个组是类似的。64.7% 无征状的病人的一个总数没有 comorbidities。心血管的疾病和肺癌症比无征状的 group.Asymptomatic COPD 组多半是更少在厨房,呼吸疾病的家庭历史和周期性的童年咳嗽里与差的通风介绍的在征兆的 COPD 病人之中是更普通的。无征状的 COPD 病人有的显著地更高的 FEV1 (73.1% 对 61.0%) , FVC (91.9% 对 82.0%) ,和 FEV1/FVC (62.9%vs.58.7%) 的更高的比率(所有 P < 0.001 ) 比征兆的组。更无征状的病人是 underdiagnosed (91.9%vs.54.3% , P < 0.001 ) 比征兆的 patients.Conclusions,这大基于人口的调查在中国证实了无征状的 COPD 病人的高流行。屏蔽测试的呼吸量测定法的更多的使用可能对 COPD 的早察觉重要。 | LU Ming YAO Wan-zhen ZHONG Nan-shan ZHOU Yu-min WANG Chen CHEN Ping KANG Jian HUANG Shao-guang CHEN Bao-yuan WANG Chang-zheng NI Dian-tao WANG Xiao-ping WANG Da-li LIU Sheng-ming Lv Jia-chun SHEN Ning DING Yan-ling RAN Pi-xin | 2010 | Chinese Medical Journal2010，，12： | 17 |
| 5 | Apelin and vascular endothelial growth factor are associated with mobilization of endothelial progenitor cells after acute myocardial infarction显示文摘This study was designed to determine the levels of early endothelial progenitor cells(EPCs),apelin,vascu-lar endothelial growth factor(VEGF) and stromal cell-derived growth factor-1(SDF-1) after acute myocardial infarction(AMI),and to investigate the relationships between these cytokines and early EPCs.Early EPCs,de-fined as CD133+,KDR+,and CD34+ cells,were quantified by flow cytometry.The levels of early EPCs and those cytokines in AMI patients were significantly different from those with coronary artery disease or controls(P < 0.05).Plasma apelin levels were inversely correlated with Gensini score and early EPCs(both P < 0.01).Early EPCs,VEGF and SDF-1 showed different patterns of changes in AMI patients during the first 24 h.The trend in the change of early EPCs was proportionally correlated with that of VEGF(P < 0.05).AMI patients exhibited in-creased early EPCs with remarkably decreased apelin levels and enhanced VEGF levels. | Jiaxin Ye Ping Ni Lina Kang Biao Xu | 2012 | The Journal of Biomedical Research2012，26，6： | 16 |
| 6 | Effect of β-sodium aescinate on hypoxia-inducible factor-1α expression in rat brain cortex after cardiopulmonary resuscitation显示文摘BACKGROUND:This study was undertaken to investigate the expression of hypoxia-inducible factor-1a(HIF-1a) in rat cerebral cortex and the effects of p-sodium aescinate(SA) administration after return of spontaneous circulation(ROSC).METHODS:Sixty rats were divided into three groups:SA group,injected intraperitoneally with SA instantly after ROSC;control group,injected intraperitoneally with normal saline;and shamoperated group,without cardiac arrest or SA.The cardiac arrest model was established using asphyxiation and intravenous potassium chloride.Blood was sampled 1,6,12,and 24 hours after ROSC.Protein and mRNA levels of HIF-1a,VEGF and EPO were detected in the cerebral cortex by immunohistochemistry and real-time RT-PCR;serum levels of NSE and S100 P were determined by enzyme-linked immunosorbent assays.RESULTS:Serum S100β and NSE were significantly increased in the control group versus the sham-operated group 1,6,12 and 24 hours after ROSC(P<0.05).Protein and mRNA levels of HIF-1a,VEGF and EPO were significantly increased in the control rats(P<0.05).Serum NSE and S100 P were significantly decreased in the SA group versus the control group 1,6,12 and 24 hours after ROSC(P<0.05).Protein and mRNA levels of HIF-1a,VEGF and EPO were significantly increased in the SA group(P<0.05).CONCLUSIONS:The expression of HIF-1a is increased in rat cerebral cortex after ROSC,and SA up-regulates the expression of HIF-1α.The up-regulation of HIF-1α improves the resistance of the cortex to ischemia and hypoxia and contributes to neuroprotection,possibly because of up-regulation of EPO and VEGF expression. | Jian Kang Ping Gong Yan-bo Ren Dong-na Gao Qiong-lei Ding | 2013 | World Journal of Emergency Medicine2013，4，1： | 10 |
| 7 | Effects of lateral ventricular transplantation of bone marrow-derived mesenchymal stem cells modified with brain-derived neurotrophic factor gene on cognition in a rat model of Alzheimer's disease显示文摘In the present study, transplantation of bone marrow-derived mesenchymal stem cells modified with brain-derived neurotrophic factor gene into the lateral ventricle of a rat model of Alzheimer's disease, resulted in significant attenuation of nerve cell damage in the hippocampal CA1 region. Furthermore, brain-derived neurotrophic factor and tyrosine kinase B mRNA and protein levels were significantly increased, and learning and memory were significantly improved. Results indicate that transplantation of bone marrow-derived mesenchymal stem cells modified with brain-derived neurotrophic factor gene can significantly improve cognitive function in a rat model of Alzheimer's disease, possibly by increasing the levels of brain-derived neurotrophic factor and tyrosine kinase B in the hippocampus. | Ping Zhang Gangyong Zhao Xianjiang Kang Likai Su | 2012 | Neural Regeneration Research2012，7，4： | 8 |
| 8 | Inflammatory pseudotumor of the liver: 13 cases of MRI findings显示文摘INTRODUCTIONInflammatory pseudotumor of the liver ,a rare benign lesion ,is often confused with the malignant tumors .Until 1998,less than 80 cases had been reported in the world [1,2].The accuracy of the pre-surgical diagnosis was low p[3,4] ,and very few materials on the diagnosis using dynamic MRI were reported [5-7] .We collected thirteen cases of inflammatory pseudotumor of the liver were collected and roved by pathology with MRI studies .Our purpose is to describe and analyze the MRI findings of this lesion and find out the valuable signs suggesting the diagnosis so as to avoid unnecessary operations. | Fu Hua Yan Kang Rong Zhou Ya Ping Jiang Wei Bin Shi Department of Radiology,Zhongshan Hospital,Medical Center of Fudan University (Former Shanghai Medical University),180 Fenglin Road,Shanghai 200032,ChinaDr.Fu Hua Yan graduated from Shanghai Medical University as a postgraduate in 1996,now an associated professor,a radiologist in chief specialized in the diagnosis of CT and MRI in hepatic diseases,having 22 papers published.She is the associated editor of the books Spiral CT and Body MRI. | 2001 | World Journal of Gastroenterology2001，7，3： | 8 |
| 9 | Causes of immune dysfunction in hyperbilirubinemia model rats显示文摘Objective:To explore the causes of immune dysfunction in neonatal rats with hyperbilirubinemia.Methods:A total of 60 newborn SD rats were equally randomized into normal saline(NS) group,LPS control group,bilirubin control group,low-dose group and high-dose group.After anesthesia,0.1 mL NS was given to the NS and LPS control group and different doses of bilirubin for the other groups;1 h later,the NS and bilirubin control group received the intraperitoneal injection of 0.05 mL NS and 1mg/kg LPS for the other groups.After 5 or 24 hours of model establishment,spleens were collected for detecting the expression levels of MyD88 and p-TAK1 protein and the spleen cells apoptosis by immunohistochemmistry and TUNEL method.After 24 hours of model establishment,scrum inflammatory factors levels and T cell subsets distribution were determined by ELISA and flow cytometry.Results:In contrast to low-dose bilirubin,high-dose bilirubin could induce spleen cells apoptosis in coordination with LPS.After 5 hours of model establishment,compared with NS group.MyD88 expression level in low-dose group elevated while p-TAK1 level in high-dose group reduced(P<0.05).In high-dose group,inflammotory factors levels and CD8^+T cells percentage were all higher than LPS control and NS group(P<0.05),while CD4^+ T cells percentage was lower than NS group(P<0.05).Conclusions:High-concentration plasma bilirubin in coordination with LPS could inhibit NF- κB signal pathways activation and aggravate inflammatory reaction,thus caused immunosuppression with inflammation cascade,which resulted in the immune dysfunction. | Xiao-Min Sun Ping Kang Ke Tao | 2015 | Asian Pacific Journal of Tropical Medicine2015，8，5： | 8 |
| 10 | Analysis and construction status of SG-Ⅱ 5PW laser facility显示文摘We present a recent progress of the SG-II 5 PW facility, which designed a multi-petawatt ultrashort pulse laser based on optical parametric chirped-pulse amplification(OPCPA). The prior two optical parametric amplifiers have been accomplished and chirped pulses with an energy of 49.7 J and a full-width-at-half-maximum(FWHM) spectrum bandwidth of 85 nm have been achieved. In the PW-scale optical parametric amplification(OPA), with the pump pulse that has an energy of 118 J from the second harmonic generation of the SG-II 7 th beam, the pump-to-signal conversion efficiency is up to 41.9%, which to the best of our knowledge is the highest among all of the reported values for OPCPA systems. The compressed pulse is higher than 37 J in 21 fs(1.76 PW), and the focal spot is ~10 μm after the closed-loop corrections by the adaptive optics. Limited by the repetition of the pump laser, the SG-II 5 PW facility operates one shot per hour. It has successfully been employed for high energy physics experiments. | Jianqiang Zhu Xinglong Xie Meizhi Sun Jun Kang Qingwei Yang Ailin Guo Haidong Zhu Ping Zhu Qi Gao Xiao Liang Ziruo Cui Shunhua Yang Cheng Zhang Zunqi Lin | 2018 | High Power Laser Science and Engineering2018，6，2： | 7 |
| 11 | Experimental Study on Preparation of Natural Gas Hydrate by Crystallization显示文摘In this paper, the saturated solution crystallization method is proposed to promote the formation of hydrate by means of the known similarities between the hydrate formation process and the crystallization process. In this method,adding the second phase crystals was used to replace the spontaneous formation of hydrate crystal nuclei to form hydrate.The effects of saturated Na_2SO_4, MgSO_4, NH_4HCO_3 and CuSO_4 solutions on the formation rates of natural gas hydrate and gas storage capacity were investigated. The results showed that the saturated solution had an influence on the hydrate formation process. Under the given experimental conditions, the saturated Na_2SO_4 solution showed a highest increase in the hydrate formation rate, and the average hydrate formation rate in its presence was 11.8 times higher than that obtained in the deionized water. Moreover, the largest formation rate of gas hydrates observed in the saturated Na_2SO_4 solution was 386 times bigger than that in the deionized water, and the gas storage capacity increased by 10 times. In addition, the average hydrate formation rate in the saturated Mg SO_4 solution was faster than that in water by 20 times. The largest formation rate of gas hydrates in the saturated MgSO_4 solution was 165 times faster than that obtained in the deionized water, and the gas storage capacity increased by 6.2 times. The saturated NH_4HCO_3 and saturated CuSO_4 solutions also influenced the formation process of hydrate. Therefore, the crystallization method of saturated solution can be used to achieve a highefficiency preparation of natural gas hydrates, which provides theoretical guidance for the storage of natural gas in the form of hydrate. | Ma Shihui Pan Zhen Li Ping Wu Yuguo Li Bingfan Kang Jinke Zhang Zhien | 2017 | China Petroleum Processing & Petrochemical Technology2017，19，1： | 7 |
| 12 | Synthesis of band gap-tunable alkali metal modified graphitic carbon nitride with outstanding photocatalytic H_2O_2 production ability via molten salt method显示文摘Band gap-tunable alkali metal modified graphitic carbon nitride was prepared by a molten salt method.X-ray diffraction, N_2 isothermal sorption, ultraviolet-visible spectroscopy, scanning electron microscope,X-ray photoelectron spectroscopy and photoluminescence were used to characterize the obtained catalysts. The photocatalytic H_2 O_2 production ability of as-prepared catalyst was investigated. The results indicate that K^+ and Na^+ are doped into g-C_3 N_4 lattice simultaneously by the molten salt method. Alkali metal modification not only promotes the specific surface area, visible light absorption and separation of electron-hole pairs, but tunes the conduction band and valence band edge positions of as-prepared catalysts by controlling the weight ratio of eutectic salts to melamine. The tunable band edge positions result in the photocatalytic H_2 O_2 production from 'single channel pathway' to 'two channel pathway',leading to the promoted H_2 O_2 production ability. | Xiaoyu Qu Shaozheng Hu Jin Bai Ping Li Guang Lu Xiaoxue Kang | 2018 | Journal of Materials Science & Technology2018，34，10： | 7 |
| 13 | Regional gray matter abnormality in hepatic myelopathy patients after transjugular intrahepatic portosystemic shunt: a voxel-based morphometry study显示文摘Hepatic myelopathy is a complication seen in patients with chronic liver failure with physiologic or iatrogenic portosystemic shunting. The main symptom is progressive lower limb dyskinesia. The role of the brain motor control center in hepatic myelopathy is unknown. This study aimed to investigate the gray matter changes in patients with hepatic myelopathy secondary to transjugular intrahepatic portosystemic shunt and to examine their clinical relevance. This was a cross-sectional study. Twenty-three liver failure patients with hepatic myelopathy(hepatic myelopathy group), 23 liver failure patients without hepatic myelopathy(non-hepatic myelopathy group) after transjugular intrahepatic portosystemic shunt, and 23 demographically matched healthy volunteers were enrolled from March 2014 to November 2016 at Xijing Hospital, Air Force Military Medical University(Fourth Military Medical University), China. High-resolution magnetization-prepared rapid gradient-echo brain imaging was acquired. Group differences in regional gray matter were assessed using voxel-based morphometry analysis. The relationship between aberrant gray matter and motor characteristics was investigated. Results demonstrated that compared with the non-hepatic myelopathy group, gray matter volume abnormalities were asymmetric, with decreased volume in the left insula(P = 0.003), left thalamus(P = 0.029), left superior frontal gyrus(P = 0.006), and right middle cingulate cortex(P = 0.021), and increased volume in the right caudate nucleus(P = 0.017), corrected with open-source software. The volume of the right caudate nucleus in the hepatic myelopathy group negatively correlated with the lower limb clinical rating of the Fugl-Meyer Assessment(r = –0.53, P = 0.01). Compared with healthy controls, patients with and without hepatic myelopathy exhibited overall increased gray matter volume in both thalami, and decreased gray matter volume in both putamen, as well as in the globus pallidus, cerebellum, and vermis. The gray matter abnormalities we found predominantly involved motor-related regions, and may be associated with motor dysfunction. An enlarged right caudate nucleus might help to predict weak lower limb motor performance in patients with preclinical hepatic myelopathy after transjugular intrahepatic portosystemic shunt. This study was approved by the Ethics Committee of Xijing Hospital, Air Force Military Medical University(Fourth Military Medical University), China(approval No. 20140227-6) on February 27, 2014. | Kang Liu Gang Chen Shu-Yao Ren Yuan-Qiang Zhu Tian-Lei Yu Ping Tian Chen Li Yi-Bin Xi Zheng-Yu Wang Jian-Jun Ye Guo-Hong Han Hong Yin | 2019 | Neural Regeneration Research2019，14，5： | 6 |
| 14 | Living near a Major Road in Beijing: Association with Lower Lung Function, Airway Acidification, and Chronic Cough显示文摘 | Zhan-Wei Hu Yan-Ni Zhao Yuan Cheng Cui-Yan Guo Xi Wang Nan Li Jun-Qing Liu Hui Kang Guo-Guang Xia Ping Hu Ping-Ji Zhang Jing Ma Ying Liu Cheng Zhang Li Su Guang-Fa Wang | 2016 | Chinese Medical Journal2016，，18： | 6 |
| 15 | Clinicopathological analysis of metaplastic meningioma: report of 15 cases in Huashan Hospital显示文摘Objective: Metaplastic meningioma is a rare subtype of benign meningiomas, classified as WHO grade I with well prognosis. Here we presented our experiences on 15 cases of metaplastic meningioma, to investigate the clinicopathological features, therapies and prognosis of these cases. Methods: 15 patients underwent surgical treatment for intracranial metaplastic meningioma between 2001 and 2010 at Neurosurgery Department of Huashan Hospital, Shanghai, China. The clinical data, radiological manifestation, treatment strategy, pathological findings and prognosis of all patients were analyzed retrospectively. Results: Among the 15 cases (10 males and 5 females), the age ranged from 22 to 74 years old (the mean age was 50.67-year old). The clinical manifestations include headache, dizziness, seizure attack, vision decrease, and weakness of bilateral lower limbs. All the patients received surgical treatment, combined with radiotherapy in some cases. In the follow-up period, recurrence occurred in 2 cases, of which 1 patient died of other system complications. Conclusions: Metaplastic meningiomas are characterized by focal or widespread mesenchymal differentiation with formation of bone, cartilage, fat, and xanthomatous tissue elements. Surgical removal is the optimal therapy, and the overall prognosis is well. But recurrence may occur in some cases, thus radiotherapy is necessary for such kind of patients. | Hailiang Tang Huaping Sun Hong Chen Ye Gong Ying Mao Qing Xie Liqian Xie Mingzhe Zheng Daijun Wang Hongda Zhu Xiaoming Che Ping Zhong Kang Zheng Shiqi Li Weimin Bao Jianhong Zhu Xuanchun Wang Xiaoyuan Feng Xiancheng Chen Liangfu Zhou | 2013 | Chinese Journal of Cancer Research2013，25，1： | 6 |
| 16 | A new flavonoid from Selaginella tamariscina显示文摘6-(2-Hydroxy-5-acetylphenyl )-apigenin (1 ) ,有一个苯基取代者的新 flavonoid,首先从 Selaginella tamariscina 被孤立。它的结构象 ESI-HR-MS 分光镜的分析一样根据 1D 和 2D NMR 被阐明。 | Jian Feng Liu Kang Ping Xu De Jian Jiang Fu Shuang Li Jian Shen Ying Jun Zhou Ping Sheng Xu Bin Tan Gui Shan Tan | 2009 | Chinese Chemical Letters2009，20，5： | 5 |
| 17 | Characterization of integrons and novel cassette arrays in bacteria from clinical isloates in China,2000-2014显示文摘Rapid dissemination of antibiotic resistance genes among bacterial isolates is an increasing problem in China.Integron,a conserved DNA sequence,which is carried on episomal genetic structures,plays a very important role in development of antibiotic resistance.This systematic analysis was based on MEDLINE and EMBASE databases.We summarized the distribution and proportion of different types of gene cassette arrays of integrons(including class 1,2,3 and atypical class 1 integron) from clinical bacteria isolates in China.Fifty-six literatures were included in this study.Most of the strains were Gram-negative bacteria(94.1%,7,364/7,822) while only 5.9% strains were Grampositive bacteria.Class 1 integrons were detected in 54.2%(3956/7295) Gram-negative strains.aadA2 was the most popular gene cassette array detected from 60 Gram-positive bacteria while dfrA17-aadA5 were detected in 426 Gramnegative bacteria.This study identified 12 novel gene cassette arrays which have not been previously found in any species.All the novel gene cassette arrays were detected from Gram-negative bacteria.A regional characteristic of distribution of integrons was presented in this study.The results highlight a need for continuous surveillance of integrons and provide a guide for future research on integron-mediated bacteria resistance. | Wenying Xia Ting Xu Tingting Qin Pengpeng Liu Yun Liu Haiquan Kang Bing Gu Ping Ma | 2016 | The Journal of Biomedical Research2016，30，4： | 5 |
| 18 | Genome wide abnormal DNA methylome of human blastocyst in assisted reproductive technology显示文摘Proper reprogramming of parental DNA methylomes is essential for mammalian embryonic development.However,it is unknown whether abnormal methylome reprogramming occurs and is associated with the failure of embryonic development.Here we analyzed the DNA methylomes of 57 blastocysts and 29 trophectoderm samples with different morphological grades during assisted reproductive technology(ART) practices.Our data reveal that the global methylation levels of high-quality blastocysts are similar(0.30 ± 0.02,mean ± SD).while the methylation levels of low-quality blastocysts are divergent and away from those of high-quality blastocysts.The proportion of blastocysts with a methylation level falling within the range of 0.30 ± 0.02 in different grades correlates with the live birth rate for that grade.Moreover,abnormal methylated regions are associated with the failure of embryonic development.Furthermore,we can use the methylation data of cells biopsied from trophectoderm to predict the blastocyst methylation level as well as to detect the aneuploidy of the blastocysts.Our data indicate that global abnormal methylome reprogramming often occurs in human embryos,and suggest that DNA methylome is a potential biomarker in blastocyst selection in ART. | Guoqiang Li Yang Yu Yong Fan Congru Li Xiaocui Xu Jialei Duan Rong Li Xiangjin Kang Xin Ma Xuepeng Chen Yuwen Ke Jie Yan Ying Lian Ping Liu Yue Zhao Hongcui Zhao Yaoyong Chen Xiaofang Sun Jianqiao Liu Jie Qiao Jiang Liu | 2017 | Journal of Genetics and Genomics2017，44，10： | 5 |
| 19 | Elevated Platelet Activating Factor Level in Ischemia-Related Arrhythmia and Its Electrophysiological Effect on Myocardium显示文摘Objective The mechanism through which platelet activating factor (PAF) induces cardiac electrical activity and arrhythmia is not well understood and previous studies have suggested a potential involvement of ion channels in its action. The present study was aimed to clarify the role of PAF in fatal arrhythmias following acute myocardia infarction (AMI) and the underlying mechanism. Methods (1) Blood PAF levels were measured among 72 AMI patients at the time of diagnosis with AMI and 48 h later, and their electrocardiogram (ECG) was recorded continuously. (2) Ischemia simulation and surface electrocardiogram were conducted in 20 pigs and their PAF levels were measured. (3) PAF perfusion and standard microelectrode recording were performed on guinea pig papillarymuscles. Results In both humans and pigs, elevated PAF levels were detected in AMI and simulated ischemia, respectively, and even higher PAF levels were found when fatal arrhythmias occurred. In guinea pig myocardium, PAF induced a shortening of action potential duration at 90% level of repolarization (APD 90 )under non-ischemic conditions and a more pronounced shortening under early simulated ischemic conditions. Conclusion AMI and ischemia are associated with increased PAF levels in humans and pigs, which are further raised when fatal arrhythmia follows. The effects of PAF on the myocardium may be mediated by multiple ion channels. | TAO Yong Kang ZHAO Shui Ping YU Pu Lin SHI Jing GU Cheng Dong SUN Hong Tao ZHANG Guo Qiang | 2013 | Biomedical and Environmental Sciences2013，26，5： | 5 |
| 20 | Study on a New Ultraviolet Sterilizer to the Surface Disinfection of the Ultrasound Probe显示文摘我们学习了消毒效果一新紫外(紫外) 超声探查上的 sterilizer 和它的利用出现。搬运人量的杀菌测试,模仿的即席试用,和有机物质影响测试被用来执行试验性的观察。人工地感染的探针为比较用 sterilizer 或一盏杀菌的灯被消毒。细菌的全部的数字和类型坚定、识别。我们的结果证明 sterilizer 在医院里在三个不同消毒方法之中有最好的消毒效果。sterilizer 没有超声探查仪器的著名损坏在一个医院背景被使用 2 年了。它有快消毒,高消毒效果,和与超声仪器的好相容性的优点,值得是在医药机构的支持的应用。 | CHEN Gui Qiu CHEN Yu Hao YI Liang YIN Jin GAO Qiong SONG Jiang Nan LI Shi Kang CHEN Pei Hou GUO Gui Ping | 2018 | Biomedical and Environmental Sciences2018，31，2： | 5 |
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